Atlantic frugivory: a plant–frugivore interaction data set for the Atlantic Forest

Non peer reviewe

Karyotype And Nucleolus Organizer Regions Of Ophisternon Aenigmaticum (teleostei: Synbranchiformes: Synbranchidae) From Venezuela (in English)

The first cytogenetic analysis by conventional Giemsa staining, silver nitrate impregnation of nucleolus organizer regions (Ag-NOR), and fluorescence in situ hybridization (FISH) carried out on Ophisternon aenigmaticum from Margarita Island, Venezuela, is reported. Two cytotypes were found: cytotype C1, 2n=46, composed of 6 medium size metacentrics, 2 subtelocentrics and 38 acrocentrics; and cytotype C2, 2n=45, composed of one large metacentric plus 5 medium size metacentrics, 1 subtelocentric and 38 acrocentric chromosomes. In cytotype C1, major ribosomal DNA (rDNA) cistrons are located in the terminal position of the short arms of the first metacentric pair as determined by Ag-NORs and FISH with the 18S rDNA probe. In cytotype C2, silver staining revealed only one positive signal in a single chromosome of the pair 1 whereas FISH with the 18S rDNA probe hybridized in two different chromosomes: One coincident with the Ag-NOR (pair 1) and other in one acrocentric chromosome, suggesting the occurrence of a peri-centric inversion. In both cytotypes, FISH with 5S rDNA probe produced hybridization signals in subtelomeric position of the short arm of the chromosome pair No. 3. Chromosome evolution in Synbranchidae is discussed.363229233Favorito-Amorim, S.A., (1998) Relações Filogenéticas da Orden Synbranchiformes e Revisão Sistemática da Familia Synbranchidae, p. 217. , Thesis. Instituto de Biociências, Universidade de São Paulo, BrazilFavorito, S.E., Zanata, A.M., Assumpção, M.I., A new Synbranchus (Teleostei: Synbranchiformes: Synbranchidae) from Ilha de Marajó, Pará, Brazil, with notes on its reproductive biology and larval development (2005) Neotrop. Ichthyol., 3, pp. 319-328Foresti, F., Oliveira, C., Tien, O.S., Cytogenetic studies of the genus Synbranchus (Pisces, Synbranchiformes, Synbranchidae) (1992) Naturalia, 17, pp. 129-138Foresti, F., Oliveira, C., Foresti De Almeida-Toledo, L., A method for chromosome preparations from large fish specimens using in vitro short-term treatment with colchicine (1993) Experientia, 49 (9), pp. 810-813Froese, R., Pauly, D., Fishbase, 2009. , www.fishbase.org, version (Cons. 10/2009)Howell, W.M., Visualization of ribosomal gene activity: silver proteins associated with rRNA transcribed from oocyte chromosomes (1977) Chromosoma, 62 (4), pp. 361-367. , DOI 10.1007/BF00327034Howell, W.M., Black, D.A., Location of the nucleolus organizer regions on the sex chromosomes of the banded killifish, Fundulus diaphanous (1979) Copeia, 3, pp. 544-546Howell, W.M., Black, D.A., Controlled silver staining of nucleolus organizer regions with a protective colloidal developer: A 1-step method (1980) Experientia, 3, pp. 1014-1015Kullander, S.O., Family synbranchidae (Swamp-eels) (2003) Check List of the Freshwater Fishes of South America, pp. 594-595. , Reis RE, Kullander SO, Ferraris CJ (Eds.), Edipucrs. Porto Alegre, BrazilLevan, A., Fredga, A., Sandburg, A., Nomenclature for centromeric position on chromosomes (1964) Hereditas, 52, pp. 201-220Long, E.O., David, I.D., Repeated genes in eukaryotes (1980) Annu. Rev. Biochem., 49, pp. 727-764Melillo, I.F.M., Foresti, F., Oliveira, C., Additional cytogenetic studies on local populations of Synbranchus marmoratus (Pisces, Synbranchiformes, Synbranchidae) (1996) Naturalia, 21, pp. 201-208Messias, L.H.V., Ferreira, D.C., Wasko, A.P., Oliveira, C., Foresti, F., Martins, C., 5S rDNA organization in the fish Synbrachus marmoratus (Synbrachidae, Synbranchiformes) (2003) Hereditas, 139 (3), pp. 228-231. , DOI 10.1111/j.1601-5223.2003.01759.xNelson, J.S., (2006) Fishes of the World, p. 601. , Wiley. New York, USAPendas, A.M., Moran, P., Freije, J.P., Garcia-Vazquez, E., Chromosomal mapping and nucleotide sequence of two tandem repeats of Atlantic salmon 5S rDNA (1994) Cytogenetics and Cell Genetics, 67 (1), pp. 31-36Pinkel, D., Straume, T., Gray, J.W., Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization (1986) Proceedings of the National Academy of Sciences of the United States of America, 83 (9), pp. 2934-2938Porto-Foresti, F., Oliveira, C., Gomes, E.A., Tabata, Y.A., Rigolino, M.G., Foresti, F., A lethal effect associated with polymorphism of the NOR-bearing chromosomes in rainbow trout (Oncorhynchus mykiss) (2004) Genetics and Molecular Biology, 27 (1), pp. 51-54Sambrook, J., Russell, D.W., (2001) Molecular Cloning: A Laboratory Manual, p. 2344. , Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, USASanchez, S., Fenocchio, A.S., Karyotypic analysis in three populations of the South-American eel like fish Synbranchus marmotatus (1996) Caryologia, 49 (1), pp. 65-71Torres, R.A., Roper, J.J., Foresti, F., Oliveira, C., Surprising genomic diversity in the Neotropical Synbranchus marmoratus (Teleostei, Synbranchidae): How many species? (2005) Neotrop. Ichthyol., 3, pp. 277-28