12 research outputs found
Beef heart mitochondria for the Rotenone monitoring.
A new procedure for the selective monitoring of the Rotenone is proposed. Since the Rotenone inhibits the first site of the mitochondrial respiratory chain in all living organisms, the proposed method is based on measurements of inhibition of the respiratory rate of beef heart mitochondria
A comparison between the responses of neutral red and acridine orange: Acridine Orange should be preferential and alternative to neutral red as a dye for the monitoring of contaminants by means of biological sensors
The acridine orange (AO) and neutral red (NR) dyes, commonly used as probes to measure the internal pH in acidic vesicles, are compared in this article. The comparison between the two dyes (arising from calculations taking into account their analytical constants) illustrated that the use of AO is preferential to that of NR because the AO response is sensitive over the whole pH range between 4.0 and 7.4, whereas the NR response is effective only between pHs 4.0 and 6.0. In addition, it became evident from the mitochondrial respiration response that NR, unlike AO, is a protonophore. When taken into consideration, these two properties suggest that AO is more suitable than NR as an indicator of toxicity measurements in water samples because the environmental toxic compounds induce pH changes in the acidic vesicles of biological structures that are used as environmental biosensors
Bidirectional fluxes of spermine across the mitochondrial membrane.
The polyamine spermine is transported into the
mitochondrial matrix by an electrophoretic mechanism
having as driving force the negative electrical membrane
potential (DW). The presence of phosphate increases
spermine uptake by reducingDpH and enhancingDW. The
transport system is a specific uniporter constituted by a
protein channel exhibiting two asymmetric energy barriers
with the spermine binding site located in the energy well
between the two barriers. Although spermine transport is
electrophoretic in origin, its accumulation does not follow
the Nernst equation for the presence of an efflux pathway.
Spermine efflux may be induced by different agents, such as
FCCP, antimycin A and mersalyl, able to completely or
partially reduce theDWvalue and, consequently, suppress
or weaken the force necessary to maintain spermine in the
matrix. However this efflux may also take place in normal
conditions when the electrophoretic accumulation of the
polycationic polyamine induces a sufficient drop inDWable
to trigger the efflux pathway. The release of the polyamine
is most probably electroneutral in origin and can take place
in exchange with protons or in symport with phosphate
anion. The activity of both the uptake and efflux pathways
induces a continuous cycling of spermine across the mitochondrial membrane, the rate of which may be prominent in imposing the concentrations of spermine in the inner and
outer compartment. Thus, this event has a significant role on
mitochondrial permeability transition modulation and consequently on the triggering of intrinsic apoptosis
A new procedure for the monitoring of cationic detergents in solution
The paper describes a new procedure for the selective monitoring of cationic surfactants in solution. The procedure is based on the fact that cationic surfactants are accumulated inside mitochondria by a potential-driven mechanism. Once inside, the surfactant induces the release of the dye Safranine, previously accumulated inside mitochondria. Therefore the monitoring consists of a direct spectrophotometric measure of the rate of release of safranine in the resuspending medium containing the cationic surfactant
Mechanism and Pathophysiological Role of Polyamine Transport in Mammalian Mitochondria. Answer to Debated Questions
Mitochondria are known to be the main players in important mitochondrial bioenergetic functions such as ATP synthesis, thermoregulatory energy dissipation, Ca2+ transport, generation of reactive oxygen species and mediation of intrinsic apoptosis. Naturally occurring polyamines, due to their high pka are almost completely protonated at physiological pH and behave as polycations in their interactions with mitochondrial membranes. Thanks to these interactions, polyamines are transported electrophoretically into the mitochondrial matrix, where they exhibit a number of effects of significant importance for the above-mentioned mitochondrial functions, particularly inner membrane permeability transition (MPT). This event is closely correlated with the intrinsic occurrence of apoptosis, so that the effect of polyamine interactions with mitochondria has important implications in the pathophysiological consequences of inducing apoptosis, i.e., protection against cancer and neurodegenerative diseases. This review also provides some answers to the old debated problems regarding the possible interactions of polyamines with mitochondrial DNA, overcoming of the Born charging energy by spermine, the \u394\u3a8 threshold value for polyamine transport, and protection of MPT by spermine in in vivo conditions. In conclusion, the old question: \u201cWhat do polyamines do?\u201d is partially solved
Milestones and recent discoveries on cell death mediated by mitochondria and their interactions with biologically active amines
Mitochondria represent cell âpowerhouses,â being involved in energy transduction from the electrochemical gradient to ATP synthesis. The morphology of their cell types may change, according to various metabolic processes or osmotic pressure. A new morphology of the inner membrane and mitochondrial cristae, significantly different from the previous one, has been proposed for the inner membrane and mitochondrial cristae, based on the technique of electron tomography. Mitochondrial Ca2+ transport (the transporter has been isolated) generates reactive oxygen species and induces the mitochondrial permeability transition of both inner and outer mitochondrial
membranes, leading to induction of necrosis and apoptosis. In the mitochondria of several cell types (liver, kidney, and heart), mitochondrial oxidative stress is an essential step in the induction of cell death, although not in brain, in which the phenomenon is caused by a different mechanism
Spermine cycling in mitochondria is mediated by adenine nucleotide translocase activity: mechanism and pathophysiological implications
Spermine, besides to be transported in mitochondria by an energy dependent electrophoretic mechanism, can be also released by two different mechanisms. The first one is induced in deenergizing conditions by FCCP or antimycin A and it is mediated by an electroneutral exchange spermine protons. The second one takes place in energizing conditions during the activity of the adenine nucleotide translocase and is mediated by an electroneutral symport mechanism involving the efflux in co-transport of spermine and phosphate and the exchange of exogenous ADP with endogenous ATP. The triggering of this mechanism permits an alternating cycling of spermine across the mitochondrial membrane, that is spermine is transported or released by energized mitochondria in the absence or presence of ATP synthesis, respectively. The physiological implications of this cycling of spermine are related to the induction or prevention of mitochondrial permeability transition and, consequently, on apoptosis or its prevention