43 research outputs found
Specific oligomerization of the 5-HT1A receptor in the plasma membrane
In the present study we analyze the oligomerization of the 5-HT1A receptor within living cells at the sub-cellular level. Using a 2-excitation Förster Resonance Energy Transfer (FRET) method combined with spectral microscopy we are able to estimate the efficiency of energy transfer based on donor quenching as well as acceptor sensitization between CFP-and YFP-tagged 5-HT1A receptors at the plasma membrane. Through the analysis of the level of apparent FRET efficiency over the various relative amounts of donor and acceptor, as well as over a range of total surface expressions of the receptor, we verify the specific interaction of these receptors. Furthermore we study the role of acylation in this interaction through measurements of a palmitoylation-deficient 5-HT1A receptor mutant. Palmitoylation increases the tendency of a receptor to localize in lipid rich microdomains of the plasma membrane. This increases the effective surface density of the receptor and provides for a higher level of stochastic interaction
The Great Markarian 421 Flare of 2010 February: Multiwavelength Variability and Correlation Studies
We report on variability and correlation studies using multiwavelength observations of the blazar Mrk 421 during the month of 2010 February, when an extraordinary flare reaching a level of âŒ27 Crab Units above 1 TeV was measured in very high energy (VHE) Îł-rays with the Very Energetic Radiation Imaging Telescope Array System (VERITAS) observatory. This is the highest flux state for Mrk 421 ever observed in VHE Îł-rays. Data are analyzed from a coordinated campaign across multiple instruments, including VHE Îł-ray (VERITAS, Major Atmospheric Gamma-ray Imaging Cherenkov), high-energy Îł-ray (Fermi-LAT), X-ray (Swift, Rossi X-ray Timing Experiment, MAXI), optical (including the GASP-WEBT collaboration and polarization data), and radio (Metsahovi, Owens Valley Radio Observatory, University of Michigan Radio Astronomy Observatory). Light curves are produced spanning multiple days before and after the peak of the VHE flare, including over several flare "decline" epochs. The main flare statistics allow 2 minute time bins to be constructed in both the VHE and optical bands enabling a cross-correlation analysis that shows evidence for an optical lag of âŒ25-55 minutes, the first time-lagged correlation between these bands reported on such short timescales. Limits on the Doppler factor (ÎŽ âȘ 33) and the size of the emission region (ÎŽ-1RBâČ 3.8 Ă 1013cm) are obtained from the fast variability observed by VERITAS during the main flare. Analysis of 10 minute binned VHE and X-ray data over the decline epochs shows an extraordinary range of behavior in the flux-flux relationship, from linear to quadratic to lack of correlation to anticorrelation. Taken together, these detailed observations of an unprecedented flare seen in Mrk 421 are difficult to explain with the classic single-zone synchrotron self-Compton model.</p
Geologia forense. Le indagini sui terreni e sui loro componenti organici ed inorganici
In ambito investigativo giudiziario si fa oggigiorno sempre piĂč uso delle tracce di terreno presenti sulla scena di un crimine per ottenere importanti informazioni sia sulla dinamica di un reato che sul suo presunto autore. Il geologo forense, specialista in questa disciplina, puĂČ di conseguenza fornire delle indicazioni utili alle indagini, che in alcune circostanze possono rivelarsi risolutive
Geologia forense. La falsificazione dei materiali geologici preziosi
Le gemme e i metalli preziosi non attirano lâuomo solo per la loro bellezza; lâalto valore economico, le piccole dimensioni e la relativa non tracciabilitĂ hanno fatto si che tali materiali siano anche estremamente allettanti per la criminalitĂ organizzata
Application of differential scanning calorimetry-chemometric coupled procedure to the evaluation of thermo-oxidation on extra virgin olive oil
In this work, the opportunity of adopting a differential
scanning calorimetry (DSC)-principal component
analysis (PCA) coupled procedure to measure the degree of
thermal stress for extra virgin olive oil (EVOO) was presented.
Oil was subjected to thermal stress under convectional
or microwave heating treatments at different heating
times up to 1,440 and 15 min, respectively, and p-anisidine
values (PAV) were obtained on all samples to measure their
oxidative degradation. The entire DSC profiles obtained on
the oil upon cooling in the range from 30 °C to â80 °C and
subsequent re-heating to 30 °C at different times and under
cooking procedures have been subjected to PCA data analysis.
PCA discriminated samples by means of profile
changes in DSC transition both upon cooling and heating
not only according to treatment times (which accounted for
the degree of thermo-oxidation) but also considering
different heating process. The proposed procedure may be
useful to measure oil thermal stress and to select appropriate
heating condition to be applied for EVOO both in industrial
and/or in food-catering sectors
A novel chemometric strategy for the estimation of extra virgin olive oil adulteration with edible oils
Abstract
A useful procedure for the qualitative and quantitative determination of
vegetable oils (canola, hazelnut, pomace and high linoleic/oleic sunflower)
as adulterants in commercial samples of extra virgin olive oil, has been
developed. Partial least squares (PLS) was employed for the analysis of
Fourier transform infrared spectroscopy (FTIR) spectral data of the blend
oil samples. Calibration models were constructed for extra virgin olive oil
purity, with wavelength selection in the infrared region, according to their
predictive ability, with first derivative and mean centering used as data
pretreatment. PLS models were internally validated by the leave-one-out
procedure. The method developed was very suitable for the determination
of modeled adulterants but it may also reveal an adulteration even if it does
not derive from the adulterants employed in this study
Application of partial least square regression to differential scanning calorimetry data for fatty acid quantitation in olive oil
A chemometric approach based on partial least (PLS) square methodology was applied to unfolded differential scanning calorimetry data obtained by 63 samples of different vegetable oils (58 extra virgin olive oils, one olive and one pomace olive oil, three seed oils) to evaluate fatty acid composition (palmitic, stearic, oleic and linoleic acids, saturated (SFA), mono (MUFA) and polysaturated (PUFA) percentages, oleic/linoleic and unsaturated/saturated ratios).
All calibration models exhibited satisfactory figures of merit. Palmitic and oleic acids, as well as SEA showed very good correlation coefficients and low root mean square error values in both calibration and validation sets. Satisfactory results were also obtained for MUFA, PUFA, stearic and linoleic acids, O/L ratio in terms of percentage recoveries and relative standard deviations. No systematic and bias errors were detected in the prediction of validation samples.
This novel approach could provide statistically similar results to those given by traditional official procedures, with the advantages of a very rapid and environmentally friendly methodology