Hsc70 is a novel interactor of NF-kappaB p65 in living hippocampal neurons

Klenke C, Widera D, Engelen T, et al. Hsc70 is a novel interactor of NF-kappaB p65 in living hippocampal neurons. PLoS ONE. 2013;8(6): e65280.Signaling via NF-κB in neurons depends on complex formation with interactors such as dynein/dynactin motor complex and can be triggered by synaptic activation. However, so far a detailed interaction map for the neuronal NF-κB is missing. In this study we used mass spectrometry to identify novel interactors of NF-κB p65 within the brain. Hsc70 was identified as a novel neuronal interactor of NF-κB p65. In HEK293 cells, a direct physical interaction was shown by co-immunoprecipitation and verified via in situ proximity ligation in healthy rat neurons. Pharmacological blockade of Hsc70 by deoxyspergualin (DSG) strongly decreased nuclear translocation of NF-κB p65 and transcriptional activity shown by reporter gene assays in neurons after stimulation with glutamate. In addition, knock down of Hsc70 via siRNA significantly reduced neuronal NF-κB activity. Taken together these data provide evidence for Hsc70 as a novel neuronal interactor of NF-κB p65

Deep Spatio-Temporal Graph Network with Self-Optimization for Air Quality Prediction

The environment and development are major issues of general concern. After much suffering from the harm of environmental pollution, human beings began to pay attention to environmental protection and started to carry out pollutant prediction research. A large number of air pollutant predictions have tried to predict pollutants by revealing their evolution patterns, emphasizing the fitting analysis of time series but ignoring the spatial transmission effect of adjacent areas, leading to low prediction accuracy. To solve this problem, we propose a time series prediction network with the self-optimization ability of a spatio-temporal graph neural network (BGGRU) to mine the changing pattern of the time series and the spatial propagation effect. The proposed network includes spatial and temporal modules. The spatial module uses a graph sampling and aggregation network (GraphSAGE) in order to extract the spatial information of the data. The temporal module uses a Bayesian graph gated recurrent unit (BGraphGRU), which applies a graph network to the gated recurrent unit (GRU) so as to fit the data’s temporal information. In addition, this study used Bayesian optimization to solve the problem of the model’s inaccuracy caused by inappropriate hyperparameters of the model. The high accuracy of the proposed method was verified by the actual PM2.5 data of Beijing, China, which provided an effective method for predicting the PM2.5 concentration

Variational Bayesian Network with Information Interpretability Filtering for Air Quality Forecasting

Air quality plays a vital role in people’s health, and air quality forecasting can assist in decision making for government planning and sustainable development. In contrast, it is challenging to multi-step forecast accurately due to its complex and nonlinear caused by both temporal and spatial dimensions. Deep models, with their ability to model strong nonlinearities, have become the primary methods for air quality forecasting. However, because of the lack of mechanism-based analysis, uninterpretability forecasting makes decisions risky, especially when the government makes decisions. This paper proposes an interpretable variational Bayesian deep learning model with information self-screening for PM2.5 forecasting. Firstly, based on factors related to PM2.5 concentration, e.g., temperature, humidity, wind speed, spatial distribution, etc., an interpretable multivariate data screening structure for PM2.5 forecasting was established to catch as much helpful information as possible. Secondly, the self-screening layer was implanted in the deep learning network to optimize the selection of input variables. Further, following implantation of the screening layer, a variational Bayesian gated recurrent unit (GRU) network was constructed to overcome the complex distribution of PM2.5 and achieve accurate multi-step forecasting. The high accuracy of the proposed method is verified by PM2.5 data in Beijing, China, which provides an effective way, with multiple factors for PM2.5 forecasting determined using deep learning technology

Variational Bayesian Network with Information Interpretability Filtering for Air Quality Forecasting

Air quality plays a vital role in people’s health, and air quality forecasting can assist in decision making for government planning and sustainable development. In contrast, it is challenging to multi-step forecast accurately due to its complex and nonlinear caused by both temporal and spatial dimensions. Deep models, with their ability to model strong nonlinearities, have become the primary methods for air quality forecasting. However, because of the lack of mechanism-based analysis, uninterpretability forecasting makes decisions risky, especially when the government makes decisions. This paper proposes an interpretable variational Bayesian deep learning model with information self-screening for PM2.5 forecasting. Firstly, based on factors related to PM2.5 concentration, e.g., temperature, humidity, wind speed, spatial distribution, etc., an interpretable multivariate data screening structure for PM2.5 forecasting was established to catch as much helpful information as possible. Secondly, the self-screening layer was implanted in the deep learning network to optimize the selection of input variables. Further, following implantation of the screening layer, a variational Bayesian gated recurrent unit (GRU) network was constructed to overcome the complex distribution of PM2.5 and achieve accurate multi-step forecasting. The high accuracy of the proposed method is verified by PM2.5 data in Beijing, China, which provides an effective way, with multiple factors for PM2.5 forecasting determined using deep learning technology

Prediction Model of Wastewater Pollutant Indicators Based on Combined Normalized Codec

Effective prediction of wastewater treatment is beneficial for precise control of wastewater treatment processes. The nonlinearity of pollutant indicators such as chemical oxygen demand (COD) and total phosphorus (TP) makes the model difficult to fit and has low prediction accuracy. The classical deep learning methods have been shown to perform nonlinear modeling. However, there are enormous numerical differences between multi-dimensional data in the prediction problem of wastewater treatment, such as COD above 3000 mg/L and TP around 30 mg/L. It will make current normalization methods challenging to handle effectively, leading to the training failing to converge and the gradient disappearing or exploding. This paper proposes a multi-factor prediction model based on deep learning. The model consists of a combined normalization layer and a codec. The combined normalization layer combines the advantages of three normalization calculation methods: z-score, Interval, and Max, which can realize the adaptive processing of multi-factor data, fully retain the characteristics of the data, and finally cooperate with the codec to learn the data characteristics and output the prediction results. Experiments show that the proposed model can overcome data differences and complex nonlinearity in predicting industrial wastewater pollutant indicators and achieve better prediction accuracy than classical models