Restitution analysis of alternans and its relationship to arrhythmogenicity in hypokalaemic Langendorff-perfused murine hearts

Alternans and arrhythmogenicity were studied in hypokalaemic (3.0 mM K+) Langendorff-perfused murine hearts paced at high rates. Epicardial and endocardial monophasic action potentials were recorded and durations quantified at 90% repolarization. Alternans and arrhythmia occurred in hypokalaemic, but not normokalaemic (5.2 mM K+) hearts (P < 0.01): this was prevented by treatment with lidocaine (10 μM, P < 0.01). Fourier analysis then confirmed transition from monomorphic to polymorphic waveforms for the first time in the murine heart. Alternans and arrhythmia were associated with increases in the slopes of restitution curves, obtained for the first time in the murine heart, while the anti-arrhythmic effect of lidocaine was associated with decreased slopes. Thus, hypokalaemia significantly increased (P < 0.05) maximal gradients (from 0.55 ± 0.14 to 2.35 ± 0.67 in the epicardium and from 0.67 ± 0.13 to 1.87 ± 0.28 in the endocardium) and critical diastolic intervals (DIs) at which gradients equalled unity (from −2.14 ± 0.52 ms to 50.93 ± 14.45 ms in the epicardium and from 8.14 ± 1.49 ms to 44.64 ± 5 ms in the endocardium). While treatment of normokalaemic hearts with lidocaine had no significant effect (P > 0.05) on either maximal gradients (0.78 ± 0.27 in the epicardium and 0.83 ± 0.45 in the endocardium) or critical DIs (6.06 ± 2.10 ms and 7.04 ± 3.82 ms in the endocardium), treatment of hypokalaemic hearts with lidocaine reduced (P < 0.05) both these parameters (1.05 ± 0.30 in the epicardium and 0.89 ± 0.36 in the endocardium and 30.38 ± 8.88 ms in the epicardium and 31.65 ± 4.78 ms in the endocardium, respectively). We thus demonstrate that alternans contributes a dynamic component to arrhythmic substrate during hypokalaemia, that restitution may furnish an underlying mechanism and that these phenomena are abolished by lidocaine, both recapitulating and clarifying clinical findings

Presynaptic External Calcium Signaling Involves the Calcium-Sensing Receptor in Neocortical Nerve Terminals

Nerve terminal invasion by an axonal spike activates voltage-gated channels, triggering calcium entry, vesicle fusion, and release of neurotransmitter. Ion channels activated at the terminal shape the presynaptic spike and so regulate the magnitude and duration of calcium entry. Consequently characterization of the functional properties of ion channels at nerve terminals is crucial to understand the regulation of transmitter release. Direct recordings from small neocortical nerve terminals have revealed that external [Ca(2+)] ([Ca(2+)](o)) indirectly regulates a non-selective cation channel (NSCC) in neocortical nerve terminals via an unknown [Ca(2+)](o) sensor. Here, we identify the first component in a presynaptic calcium signaling pathway.By combining genetic and pharmacological approaches with direct patch-clamp recordings from small acutely isolated neocortical nerve terminals we identify the extracellular calcium sensor. Our results show that the calcium-sensing receptor (CaSR), a previously identified G-protein coupled receptor that is the mainstay in serum calcium homeostasis, is the extracellular calcium sensor in these acutely dissociated nerve terminals. The NSCC currents from reduced function mutant CaSR mice were less sensitive to changes in [Ca(2+)](o) than wild-type. Calindol, an allosteric CaSR agonist, reduced NSCC currents in direct terminal recordings in a dose-dependent and reversible manner. In contrast, glutamate and GABA did not affect the NSCC currents.Our experiments identify CaSR as the first component in the [Ca(2+)](o) sensor-NSCC signaling pathway in neocortical terminals. Decreases in [Ca(2+)](o) will depress synaptic transmission because of the exquisite sensitivity of transmitter release to [Ca(2+)](o) following its entry via voltage-activated Ca(2+) channels. CaSR may detects such falls in [Ca(2+)](o) and increase action potential duration by increasing NSCC activity, thereby attenuating the impact of decreases in [Ca(2+)](o) on release probability. CaSR is positioned to detect the dynamic changes of [Ca(2+)](o) and provide presynaptic feedback that will alter brain excitability

Developments in the remote sensing of soil erosion in the perspective of sub-Saharan Africa. Implications on future food security and biodiversity

The remote sensing of soil erosion has gained substantial consideration, with considerable scientific research work having been conducted in the past, due to technological improvements that have resulted in the release of robust, cheap and high resolution datasets with a global foot-print. This paper reviews developments in the application of remote sensing technologies in sub-Saharan Africa with a explicit emphasis on soil erosion monitoring. Soil loss due to soil erosion by water has been identified by African geomorphologists, environmentalists and governments, as the primary threat to agriculture, biodiversity and food security across the continent. The article offers a detailed review of the progress in the remote sensing as it summarises research work that have been conducted, using various remote sensing sensors and platforms and further evaluates the significance of variations in sensor resolutions and data availability for sub-Saharan Africa. Explicit application examples are used to highlight and outline this progress. Although some progress has been made, this review has revealed the necessity for further remote sensing work to provide time-series soil erosion modelling and its implications on future food security and biodiversity in the face of changing climate and food insecurity. Overall, this review have shown the immediate need for a drastical move towards the use of new generation sensors with a plausible spatial, temporal characteristics and more importantly a global foot-print

Effects of rock fragments incorporated in the soil matrix on concentrated flow hydraulics and erosion

Rock fragments can act as a controlling factor for erosional rates and patterns in the landscape. Thus, the objective of this study is to better understand the role that rock fragments incorporated into the soil matrix play in concentrated flow hydraulics and erosion. Laboratory flume experiments were conducted with soil material that was mixed with rock fragments. Rock fragment content ranged from 0 to 40 per cent by volume. Other treatments were slope (7 and 14%) and flow discharge (5(.)7 and 11(.)41 min(-1)). An increase in rock fragment content resulted in lower sediment yield, and broader width of How. Rock fragment cover at the soil surface, i.e. surface armour, increased with time in experiments with rock fragments. Flow energy was largely dissipated by rock fragment cover. For more turbulent flow conditions, when roughness elements were submerged in the flow, hydraulic roughness was similar for different rock fragment contents. In experiments with few or no rock fragments a narrow rill incised. Flow energy was dissipated by headcuts. Total sediment yield was much larger than for experiments with rock fragments in the soil. Adding just a small number of rock fragments in the soil matrix resulted in a significant reduction of sediment yield. Copyright (c) 2007 John Wiley & Sons, Ltd.status: publishe