Genetic Diversity and Phylogeny of Antagonistic Bacteria against Phytophthora nicotianae Isolated from Tobacco Rhizosphere

The genetic diversity of antagonistic bacteria from the tobacco rhizosphere was examined by BOXAIR-PCR, 16S-RFLP, 16S rRNA sequence homology and phylogenetic analysis methods. These studies revealed that 4.01% of the 6652 tested had some inhibitory activity against Phytophthora nicotianae. BOXAIR-PCR analysis revealed 35 distinct amplimers aligning at a 91% similarity level, reflecting a high degree of genotypic diversity among the antagonistic bacteria. A total of 25 16S-RFLP patterns were identified representing over 33 species from 17 different genera. Our results also found a significant amount of bacterial diversity among the antagonistic bacteria compared to other published reports. For the first time; Delftia tsuruhatensis, Stenotrophomonas maltophilia, Advenella incenata, Bacillus altitudinis, Kocuria palustris, Bacillus licheniformis, Agrobacterium tumefaciens and Myroides odoratimimus are reported to display antagonistic activity towards Phytophthora nicotianae. Furthermore, the majority (75%) of the isolates assayed for antagonistic activity were Gram-positives compared to only 25% that were Gram-negative bacteria

Introduction: thinking about Caribbean media worlds

This special issue brings together cultural studies of media with current themes in Caribbean studies and anthropology. The papers were part of an interdisciplinary conference panel focused upon Caribbean Media Worlds. At the outset, we wanted to demonstrate that there are several specific reasons why the Caribbean makes a particularly interesting case study for examining the cultural practices, relationships, micro-political encounters and identities that surround the distribution and use of media technologies. The collection here examines media in interaction with the world of which it is part — in this case, that world is imagined as `the Caribbean'. The main goal of this introduction is to contextualize the studies by presenting key ideas within Caribbean research as a backdrop against which the conceptual and analytic frameworks which emerge in the contributors' articles can be better understood

Megalopolis 50 Years On: The Transformation of a City Region

This article examines Megalopolis 50 years after Gottmann's seminal study of the most urbanized region of the US Eastern Seaboard. His study provides an invaluable datum point, and we use it as a benchmark for reexamining the socio-spatial transformations of a city region. After redefining Megalopolis and showing major aggregate trends since 1950, we analyze 39 selected variables for place level census data for 2,353 places to perform a principal components analysis (PCA). Our analysis shows that Megalopolis remains a significant center for the nation's population and economic activity. A half century of urban restructuring demonstrates that the forces of urban decentralization have made the region a more fully suburbanized agglomeration. We reveal a complex socioeconomic pattern of a vast urban area structured by class, education, housing tenure, housing age, and race and ethnicity. The cluster analysis reveals five distinct clusters of urban places identified by our PCA: 'affluent places', 'places of poverty', 'Black middle class places', 'immigrant gateway places' and 'middle America places'. Copyright (c) 2007 The Authors. Journal Compilation (c) 2007 Joint Editors and Blackwell Publishing Ltd.

Especiarias como antioxidantes naturais: aplicações em alimentos e implicação na saúde Spices as natural antioxidants: their application in food and implication for health

O crescente interesse na substituição de antioxidantes sintéticos por naturais em alimentos tem fomentado a pesquisa sobre fontes vegetais, caracterização de matérias-primas e identificação de novos compostos antioxidantes. As reações de oxidação não são uma preocupação exclusiva das indústrias alimentícias, seu estudo é também amplamente necessário para evitar implicações indesejáveis na saúde humana. O objetivo desta revisão, depois de apresentar aspectos gerais sobre a oxidação, é concentrar-se nos benefícios da utilização das especiarias como antioxidantes naturais, em especial as da família Labiatae, amplamente utilizadas na culinária brasileira.<br>The growing interest in replacing synthetic antioxidants by natural antioxidants in food has stimulated research on vegetable sources, raw material characterization and identification of new antioxidant compounds. Oxidation reactions are not an exclusive preoccupation of the food industry; studies regarding this issue are widely necessary in order to prevent undesired implications for human health. The aim of this review, after presenting overall aspects about oxidation, is to concentrate on the benefits of using spices as natural antioxidants, especially those from the Labiatae family, which are widely used in the Brazilian cuisine