preoperative c reactive protein and thrombocyte count as potential markers for longterm survival in ovarian cancer

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Episodi di mortalit\ue0 in storioni (Acipenser spp.) d\u2019allevamento

During 2004-spring 2008, 21 bacterial outbreaks in farmed Siberian sturgeon (Acipenser baeri), 3 in Russian sturgeon (A. gueldenstaedti) and 2 in hydrid sturgeons with different mortality, were reported in Northern Italy. The bacterial infections isolated were caused by Aeromonas hydrophila (12 cases), Pseudomonas fluorescens (7 cases), A. sobria (5 cases), Plesiomonas shigelloides (1 case) and Photobacterium damselae subsp. damselae (1 case). In all cases, the characteristic symptoms were the presence of hemorrhagic enteritis in the terminal tract of the intestine

Mycobacterium marinum in Italian farmed striped bass: anatomopathological and histological features

Striped bass (hybrid of Morone ssp.) is a fish raised in hot water; in Italy is not very common because its meat is not so appreciate. In USA and Israel M. marinum, M. shottii, M. pseudoshottii were the mycobacterial species more frequently isolated from Morone spp. The aim of the present work is to describe the first M. marinum infection in a striped bass farm of Northern Italy. In summer 2007 some gills of striped bass with yellow-brown nodules were sent to Istituto Zooprofilattico and the histopathological examination showed several granulomas with a large number of acid-fast bacilli. After this finding, some fishes from the same farm were randomly selected, sacrificed and necropsied in order to identify the mycobacterial species. The anatomopathological exams revealed lesions in various organs of all animals: ulcerative cutaneous lesions, yellow mucus with red nodules on the gills, splenomegaly and military granulomatous lesions in liver, kidney and spleen. At necropsy, liver, spleen, kidney, gills and muscle were collected and a portion fixed in formalin for the histological and immunohistochemical examination while another portion, not fixed, utilized for parasitological, virological and bacteriological investigations. Microscopically granulomas presented a central necrotic area surrounded by inflammatory cells and enclosed by a thin capsule. All granulomas were associated with a great number of acid-fast bacilli resulting positive to immunohistochemistry with a polyclonal antibody against M. bovis. From all samples, with the exception of muscle, it was possible to isolate photochromogenic colonies and the purified isolates were characterised by phenotypical and biochemical identification. All the isolates were identified as M. marinum. It is important to emphasize that, although granulomatous lesions were diffused in various organs, no fishes died for the infection and that the low death rate allowed the increase of human risk by manipulation and consumption of contaminated fishes

Infezioni batteriche in storioni (Acipenser spp.) d'allevamento: casistica nel triennio 2005-2007

Viene presentata un'ampia casistica raccolta dal 2005 al 2007 in relazioni alle principali infezioni batteriche rilevate in storioni allevati in Italia, esprimendo considerazioni sui fattori di rischio di carattere ambientale e gestionale che possono condizionare l'emergenza di malattie batteriche in allevamento

Axillary surgery after neoadjuvant therapy in initially node-positive breast cancer: international EUBREAST survey

Background There is no consensus on axillary management after neoadjuvant therapy (NAT) in patients with clinically node-positive (cN+) breast cancer. To investigate current clinical practice, an international survey was conducted among breast surgeons and radiation oncologists. The aim of the first part of the survey was to provide a snapshot of international discrepancies regarding axillary surgery in this context. Methods The European Breast Cancer Research Association of Surgical Trialists (EUBREAST) developed a web-based survey containing 39 questions describing clinical scenarios in the setting of axillary management in patients with cN1 disease converting to ycN0 after NAT. The survey was then distributed to breast surgeons and radiation oncologists via 14 breast cancer societies between April and October 2021. Results Responses from 349 physicians in 45 countries were recorded. The most common post-NAT axillary surgery in patients with cN1 disease converting to ycN0 was targeted axillary dissection (54.2 per cent), followed by sentinel lymph node biopsy (SLNB) alone (20.9 per cent), level 1-2 axillary lymph node dissection (ALND) (18.4 per cent), level 1-3 ALND (4 per cent), and targeted lymph node biopsy (2.5 per cent). For SLNB alone, dual tracers were most commonly used (62.3 per cent). Management varied widely in patients with ambiguous axillary status before initiation of treatment or a residual metastatic burden in the axilla after NAT. In patients with ycN+ tumours, ALND was the preferred surgical approach for 66.8 per cent of respondents. Conclusion These results highlight the wide heterogeneity in surgical approaches to the axilla after NAT. To standardize the guidelines, further data from clinical research are urgently needed, which underlines the importance of the ongoing AXSANA (EUBREAST-3) study.Several discrepancies in the surgical approach to the axilla in patients with cN+ disease converting to ycN0 exist. The most common axillary surgical approaches in patients with cN1 tumours converting to ycN0 are targeted axillary dissection (TAD) and sentinel lymph node biopsy. In targeted lymph node biopsy/TAD, there was a wide heterogeneity with regard to localization techniques

Axillary surgery after neoadjuvant therapy in initially node-positive breast cancer: international EUBREAST survey

Background There is no consensus on axillary management after neoadjuvant therapy (NAT) in patients with clinically node-positive (cN+) breast cancer. To investigate current clinical practice, an international survey was conducted among breast surgeons and radiation oncologists. The aim of the first part of the survey was to provide a snapshot of international discrepancies regarding axillary surgery in this context. Methods The European Breast Cancer Research Association of Surgical Trialists (EUBREAST) developed a web-based survey containing 39 questions describing clinical scenarios in the setting of axillary management in patients with cN1 disease converting to ycN0 after NAT. The survey was then distributed to breast surgeons and radiation oncologists via 14 breast cancer societies between April and October 2021. Results Responses from 349 physicians in 45 countries were recorded. The most common post-NAT axillary surgery in patients with cN1 disease converting to ycN0 was targeted axillary dissection (54.2 per cent), followed by sentinel lymph node biopsy (SLNB) alone (20.9 per cent), level 1-2 axillary lymph node dissection (ALND) (18.4 per cent), level 1-3 ALND (4 per cent), and targeted lymph node biopsy (2.5 per cent). For SLNB alone, dual tracers were most commonly used (62.3 per cent). Management varied widely in patients with ambiguous axillary status before initiation of treatment or a residual metastatic burden in the axilla after NAT. In patients with ycN+ tumours, ALND was the preferred surgical approach for 66.8 per cent of respondents. Conclusion These results highlight the wide heterogeneity in surgical approaches to the axilla after NAT. To standardize the guidelines, further data from clinical research are urgently needed, which underlines the importance of the ongoing AXSANA (EUBREAST-3) study.Several discrepancies in the surgical approach to the axilla in patients with cN+ disease converting to ycN0 exist. The most common axillary surgical approaches in patients with cN1 tumours converting to ycN0 are targeted axillary dissection (TAD) and sentinel lymph node biopsy. In targeted lymph node biopsy/TAD, there was a wide heterogeneity with regard to localization techniques

Axillary surgery after neoadjuvant therapy in initially node-positive breast cancer: international EUBREAST survey

Background There is no consensus on axillary management after neoadjuvant therapy (NAT) in patients with clinically node-positive (cN+) breast cancer. To investigate current clinical practice, an international survey was conducted among breast surgeons and radiation oncologists. The aim of the first part of the survey was to provide a snapshot of international discrepancies regarding axillary surgery in this context. Methods The European Breast Cancer Research Association of Surgical Trialists (EUBREAST) developed a web-based survey containing 39 questions describing clinical scenarios in the setting of axillary management in patients with cN1 disease converting to ycN0 after NAT. The survey was then distributed to breast surgeons and radiation oncologists via 14 breast cancer societies between April and October 2021. Results Responses from 349 physicians in 45 countries were recorded. The most common post-NAT axillary surgery in patients with cN1 disease converting to ycN0 was targeted axillary dissection (54.2 per cent), followed by sentinel lymph node biopsy (SLNB) alone (20.9 per cent), level 1-2 axillary lymph node dissection (ALND) (18.4 per cent), level 1-3 ALND (4 per cent), and targeted lymph node biopsy (2.5 per cent). For SLNB alone, dual tracers were most commonly used (62.3 per cent). Management varied widely in patients with ambiguous axillary status before initiation of treatment or a residual metastatic burden in the axilla after NAT. In patients with ycN+ tumours, ALND was the preferred surgical approach for 66.8 per cent of respondents. Conclusion These results highlight the wide heterogeneity in surgical approaches to the axilla after NAT. To standardize the guidelines, further data from clinical research are urgently needed, which underlines the importance of the ongoing AXSANA (EUBREAST-3) study.Several discrepancies in the surgical approach to the axilla in patients with cN+ disease converting to ycN0 exist. The most common axillary surgical approaches in patients with cN1 tumours converting to ycN0 are targeted axillary dissection (TAD) and sentinel lymph node biopsy. In targeted lymph node biopsy/TAD, there was a wide heterogeneity with regard to localization techniques

The Coup-TFII orphan nuclear receptor is an activator of the γ-globin gene

The human fetal γ-globin gene is repressed in the adult stage through complex regulatory mechanisms involving transcription factors and epigenetic modifiers. Reversing γ-globin repression, or maintaining its expression by manipulating regulatory mechanisms, has become a major clinical goal in the treatment of β-hemoglobinopathies. Here, we identify the orphan nuclear receptor Coup-TFII (NR2F2/ARP-1) as an embryonic/fetal stage activator of γ-globin expression. We show that Coup-TFII is expressed in early erythropoiesis of yolk sac origin, together with embryonic/fetal globins. When overexpressed in adult cells (including peripheral blood cells from human healthy donors and β039 thalassemic patients) Coup-TFII activates the embryonic/fetal globins genes, overcoming the repression imposed by the adult erythroid environment. Conversely, the knock-out of Coup-TFII increases the β/γ+β globin ratio. Molecular analysis indicates that Coup-TFII binds in vivo to the β-locus and contributes to its conformation. Overall, our data identify Coup-TFII as a specific activator of the γ-globin gene

Organ preconditioning: the past, current status, and related lung studies

Preconditioning (PC) has emerged as a powerful method for experimentally and clinically attenuating various types of organ injuries. In this paper related clinical and basic research issues on organ preconditioning issues were systemically reviewed. Since lung injuries, including ischemia-reperfusion and others, play important roles in many clinical results, including thromboembolism, trauma, thermal injury, hypovolemic and endotoxin shock, reimplantation response after organ transplantation, and many respiratory diseases in critical care. It is of interest to uncover methods, including the PCs, to protect the lung from the above injuries. However, related studies on pulmonary PC are relatively rare and still being developed, so we will review previous literature on experimental and clinical studies on pulmonary PC in the following paragraphs