A 15.7-minAM CVn binary discovered in K2

We present the discovery of SDSS J135154.46−064309.0, a short-period variable observed using 30-mincadence photometry in K2 Campaign 6. Follow-up spectroscopy and high-speed photometry support a classification as a new member of the rare class of ultracompact accreting binaries known as AM CVn stars. The spectroscopic orbital period of 15.65 ± 0.12 min makes this system the fourth-shortest-period AM CVn known, and the second system of this type to be discovered by the Kepler spacecraft. The K2 data show photometric periods at 15.7306 ± 0.0003 min, 16.1121 ± 0.0004 min, and 664.82 ± 0.06 min, which we identify as the orbital period, superhump period, and disc precession period, respectively. From the superhump and orbital periods we estimate the binary mass ratio q = M2/M1= 0.111 ± 0.005, though this method of mass ratio determination may not be well calibrated for helium-dominated binaries. This system is likely to be a bright foreground source of gravitational waves in the frequency range detectable by Laser Interferometer Space Antenna, and may be of use as a calibration source if future studies are able to constrain the masses of its stellar components

System parameters of three short-period cataclysmic variable stars

Using photometric ULTRACAM observations of three new short period cataclysmic variables, we model the primary eclipse lightcurves to extract the orbital separation, masses, and radii of their component stars. We find donor masses of 0.060 +/- 0.008 solar masses, 0.042 +/- 0.001 solar masses, and 0.042 +/- 0.004 solar masses, two being very low-mass sub-stellar donors, and one within 2 sigma of the hydrogen burning limit. All three of the new systems lie close to the modified, "optimal" model evolutionary sequence of Knigge et al. (2011). We briefly re-evaluate the long-standing discrepancy between observed donor mass and radius data, and theoretical CV evolutionary tracks. By looking at the difference in the observed period at each mass and the period predicted by the Knigge et al. (2011) evolutionary sequence, we qualitatively examine the form of excess angular momentum loss that is missing from the models below the period gap. We show indications that the excess angular momentum loss missing from CV models grows in importance relative to gravitational losses as the period decreases. Detailed CV evolutionary models are necessary to draw more quantitative conclusions in the future

Characterising eclipsing white dwarf M dwarf binaries from multi-band eclipse photometry

With the prevalence of wide-field, time-domain photometric sky surveys, the number of eclipsing white dwarf systems being discovered is increasing dramatically. An efficient method to follow these up will be key to determining any population trends and finding any particularly interesting examples. We demonstrate that multi-band eclipse photometry of binaries containing a white dwarf and an M dwarf can be used to determine the masses and temperatures of the white dwarfs to better than 5 per cent. For the M dwarfs we measure their parameters to a precision of better than 6 per cent with the uncertainty dominated by the intrinsic scatter of the M dwarf mass-radius relationship. This precision is better than what can typically be achieved with low-resolution spectroscopy. The nature of this method means that it will be applicable to LSST data in the future, enabling direct characterisation without follow-up spectroscopy. Additionally, we characterise three new post-common-envelope binaries from their eclipse photometry, finding two systems containing hot helium-core white dwarfs with low-mass companions (one near the brown dwarf transition regime) and a possible detached cataclysmic variable at the lower edge of the period gap

Spectroscopic and photometric periods of six ultracompact accreting binaries

Ultracompact accreting binary systems each consist of a stellar remnant accreting helium-enriched material from a compact donor star. Such binaries include two related sub-classes, AM CVn-type binaries and helium cataclysmic variables, in both of which the central star is a white dwarf. We present a spectroscopic and photometric study of six accreting binaries with orbital periods in the range of 40--70 min, including phase-resolved VLT spectroscopy and high-speed ULTRACAM photometry. Four of these are AM CVn systems and two are helium cataclysmic variables. For four of these binaries we are able to identify orbital periods (of which three are spectroscopic). SDSS J1505+0659 has an orbital period of 67.8 min, significantly longer than previously believed, and longer than any other known AM CVn binary. We identify a WISE infrared excess in SDSS J1505+0659 that we believe to be the first direct detection of an AM CVn donor star in a non-direct impacting binary. The mass ratio of SDSS J1505+0659 is consistent with a white dwarf donor. CRTS J1028-0819 has an orbital period of 52.1 min, the shortest period of any helium cataclysmic variable. MOA 2010-BLG-087 is co-aligned with a K-class star that dominates its spectrum. ASASSN-14ei and ASASSN-14mv both show a remarkable number of echo outbursts following superoutbursts (13 and 10 echo outbursts respectively). ASASSN-14ei shows an increased outburst rate over the years following its superoutburst, perhaps resulting from an increased accretion rate

Putting the Customer Back in the Center of SOA with Service Design and User-Centered Design

This article introduces a methodology used for designing the online presence of a Swiss SME providing Cloud Services. The Web application used for the purchasing and administration, backed by a Service-Oriented Architec-ture (SOA), has been designed to be customer-centric using a combination of different techniques borrowed from the fields of ethnomethodology, service de-sign and user-centered design. The tools employed include service blueprint de-sign and affinity diagram analysis followed by prototyping and subsequent usa-bility evaluation. This collaborative methodology explained with the help of the applied research project use case is seen to yield excellent results in terms of customer-orientation

Rapid decline in estimated glomerular filtration rate in sickle cell anemia: Results of a multicenter pooled analysis

Chronic kidney disease (CKD), typically defined as kidney damage or decreased kidney function for 3 or more months, is common in sickle cell disease (SCD). Increasing evidence suggests that the glomerulopathy of SCD is progressive. CKD is associated with increased mortality in SCD. Based on single center studies, we previously reported on the high prevalence of rapid decline in kidney function, defined as estimated glomerular filtration rate (eGFR) loss >3.0 mL/min/1.73 m2per year, in SCD. In the present study, we further examine rapid eGFR decline in sickle cell anemia, using a pooled analysis of patients to better characterize factors associated with such decline and its association with mortality

Long-term photometric monitoring and spectroscopy of the white dwarf pulsar AR Scorpii

AR Scorpii (AR Sco) is the only radio-pulsing white dwarf known to date. It shows a broad-band spectrum extending from radio to X-rays whose luminosity cannot be explained by thermal emission from the system components alone, and is instead explained through synchrotron emission powered by the spin-down of the white dwarf. We analysed NTT/ULTRACAM, TNT/ULTRASPEC, and GTC/HiPERCAM high-speed photometric data for AR Sco spanning almost seven years and obtained a precise estimate of the spin frequency derivative, now confirmed with 50-σ significance. Using archival photometry, we show that the spin-down rate of P/P˙=5.6×106 years has remained constant since 2005. As well as employing the method of pulse-arrival time fitting used for previous estimates, we also found a consistent value via traditional Fourier analysis for the first time. In addition, we obtained optical time-resolved spectra with WHT/ISIS and VLT/X-shooter. We performed modulated Doppler tomography for the first time for the system, finding evidence of emission modulated on the orbital period. We have also estimated the projected rotational velocity of the M-dwarf as a function of orbital period and found that it must be close to Roche lobe filling. Our findings provide further constraints for modelling this unique system

“Wax On, Wax Off”: In Vivo Imaging of Plant Physiology and Disease with Fourier Transform Infrared Reflectance Microspectroscopy

Analysis of the epicuticular wax layer on the surface of plant leaves can provide a unique window into plant physiology and responses to environmental stimuli. Well-established analytical methodologies can quantify epicuticular wax composition, yet few methods are capable of imaging wax distribution in situ or in vivo. Here, the first report of Fourier transform infrared (FTIR) reflectance spectroscopic imaging as a non-destructive, in situ, method to investigate variation in epicuticular wax distribution at 25 µm spatial resolution is presented. The authors demonstrate in vivo imaging of alterations in epicuticular waxes during leaf development and in situ imaging during plant disease or exposure to environmental stressors. It is envisaged that this new analytical capability will enable in vivo studies of plants to provide insights into how the physiology of plants and crops respond to environmental stresses such as disease, soil contamination, drought, soil acidity, and climate change

Longitudinal study of glomerular hyperfiltration in adults with sickle cell anemia: a multicenter pooled analysis

Glomerular hyperfiltration is common in young sickle cell anemia patients and precedes development of overt kidney disease. In this multicenter pooled cohort, we characterized hyperfiltration and its decline to normal range in adult patients. Glomerular filtration rate (GFR) was estimated using the creatinine-based 2009 CKD-EPI (Chronic Kidney Disease Epidemiology Collaboration) equation omitting race adjustment and the 2021 CKD-EPI equation. Using CKD-EPI–2009, 506 patients had baseline estimated GFR (eGFR) $90 mL/min per 1.73 m2, median age of 24 (interquartile range [IQR], 19-34) years and 5.17 years of follow-up. The prevalence of hyperfiltration (eGFR$140 and $130 mL/min per 1.73 m2 for men and women, respectively) was 38.3%. Using CKD-EPI–2009, baseline hyperfiltration was less likely with older age (odds ratio [OR], 0.78; 95% confidence interval [CI], 0.73-0.83; P, .0001), male sex (OR, 0.32; 95% CI, 0.18-0.58; P 5 .0002), and higher weight (OR, 0.96; 95% CI, 0.94-0.99; P 5 .001). Using CKD-EPI–2021, hyperfiltration was similarly less likely with older age (OR, 0.75; 95% CI, 0.70-0.81; P, .0001), male sex (OR, 0.24; 95% CI, 0.13-0.44; P, .0001), and higher weight (OR, 0.97; 95% CI, 0.95-0.99; P 5 .004). In patients with baseline hyperfiltration, eGFR declined to normal values at a median age of 26.2 years. Using CKD-EPI–2009, this decline was associated with male sex (HR, 2.20; 95% CI, 1.26-3.87; P 5 .006), systolic blood pressure (hazard ratio [HR], 1.02; 95% CI, 1.01-1.04; P 5 .01), and hydroxyurea use (HR, 1.74; 95% CI, 1.002-3.03; P 5 .05). Using CKD-EPI–2021, decline of eGFR to normal was only associated with male sex (HR, 3.39; 95% CI, 2.01-5.69; P, .0001). Decline to normal eGFR range from hyperfiltration occurs earlier in males, those on hydroxyurea, and with higher systolic blood pressure

Sample preparation with sucrose cryoprotection dramatically alters Zn distribution in the rodent hippocampus, as revealed by elemental mapping

Transition metal ions (Fe, Mn, Cu, Zn) are essential for healthy brain function, but altered concentration, distribution, or chemical form of the metal ions has been implicated in numerous brain pathologies. Currently, it is not possible to image the cellular or sub-cellular distribution of metal ions in vivo and therefore, studying brain-metal homeostasis largely relies on ex vivo in situ elemental mapping. Sample preparation methods that accurately preserve the in vivo elemental distribution are essential if one wishes to translate the knowledge of elemental distributions measured ex vivo toward increased understanding of chemical and physiological pathways of brain disease. The choice of sample preparation is particularly important for metal ions that exist in a labile or mobile form, for which the in vivo distribution could be easily distorted by inappropriate sample preparation. One of the most widely studied brain structures, the hippocampus, contains a large pool of labile and mobile Zn. Herein, we describe how sucrose cryoprotection, the gold standard method of preparing tissues for immuno-histochemistry or immuno-fluorescence, which is also often used as a sample preparation method for elemental mapping studies, drastically alters hippocampal Zn distribution. Based on the results of this study, in combination with a comparison against the strong body of published literature that has used either rapid plunge freezing of brain tissue, or sucrose cryo-protection, we strongly urge investigators in the future to cease using sucrose cryoprotection as a method of sample preparation for elemental mapping, especially if Zn is an analyte of interest