33 research outputs found
The role of myocardial membrane proteins and myocardial oedema in postoperative myocardial dysfunction
The vast majority of children undergoing surgical repair of cardiac lesions do spectacularly well. However a significant proportion, ~ 25%, struggle to progress in the early postoperative period and require additional pharmacological and occasionally mechanical circulatory support. All children typically have some degree of postoperative myocardial dysfunction, with the severe spectrum termed the low cardiac output state (LCOS). LCOS is clinically defined as the requirement for new or escalated inotrope therapy, a widened arteriovenous oxygen difference, cardiac arrest or the need for reinstitution of mechanical circulatory support. LCOS is largely responsible for the morbidity and mortality involved in paediatric cardiac surgery. Despite the predictability of LCOS in the initial postoperative hours, the underlying pathophysiology remains unclear. The period of decline in cardiac function that typifies LCOS is temporally associated with the development of oedema in the tissues of the body, including the heart. This relationship between oedema and dysfunction has increasingly become blurred, with a tendency to elevate the temporal association to a causal link. We sought to explore the causes and contributions to myocardial dysfunction in this setting, including the roles of oedema and ischaemia within the heart. In focusing on oedema and ischaemia we also examined the effects of these insults on relevant myocardial membrane proteins, including those that permit rapid water transport – aquaporins (AQPs), and those involved in membrane mechanics – dystrophin, and membrane repair – dysferlin. Experimental settings which enabled the in vitro dissection of these insults and proteins of interest were combined with a clinically accurate in vivo model. This thesis describes a series of thematically linked experiments that examined LCOS, myocardial oedema and the role of various membrane proteins. We performed isolated cardiomyocyte studies, isolated heart studies as well as a clinically relevant large animal (lamb) cardiopulmonary bypass (CPB) model. Across these models we also explored the role of therapeutically protecting myocardial membranes with Poloxamer 188 (P188) and assessed any influence on myocardial function, oedema and membrane proteins. vi The results from these three models suggest that the clinically accepted dogma of a causative link between myocardial oedema and dysfunction overstates the contribution of myocardial oedema to LCOS. We found that ischaemia/reperfusion was of primary importance in causing myocardial dysfunction. Myocardial oedema without ischaemia had a mild and reversible contribution to myocardial dysfunction, but this was minor in comparison to the gross dysfunction attributable to ischaemia. Isolated cardiomyocytes, with induced oedema, functioned well. Whilst ischaemic cardiomyocytes, with less swelling still had severe contractile dysfunction. Isolated hearts, perfused with an oedema inducing crystalloid perfusate developed myocardial oedema and had minimal reversible systolic and diastolic dysfunction. Isolated hearts which experienced global ischaemia had comparable degrees of myocardial oedema, and significantly greater degrees of myocardial dysfunction that increased in severity with increasing duration of ischaemia. In the lamb CPB model, only those lambs which underwent aortic cross clamping and had a period of ischaemia had poor myocardial function. These lambs also had swollen hearts, raised myocardial AQP1 mRNA and reduced membrane dysferlin protein expression. Membrane dystrophin protein expression was not altered, somewhat unexpectedly with CPB with or without ischaemia. Lambs placed on CPB without ischaemia had good myocardial function, minimal oedema and unchanged membrane protein expression during the survival period. In a blinded lamb CPB trial of P188 there were improved haemodynamics and indicies of myocardial function associated with its use. This was also associated with preservation of dysferlin expression and reduced membrane injury. In parallel isolated heart trials of this therapy, there was a reduction in myocardial oedema associated with its use in non-ischaemic experiments. There was also a suggestion of improved diastolic function in ischaemic experiments, but no change in myocardial water content. In conclusion, we have highlighted the primacy of ischaemia/reperfusion over oedema in contributing to LCOS. We have refuted the accepted dogma that myocardial oedema causes significant dysfunction in itself, with important oedema likely to result from ischaemia. We have shown that AQP1 may be involved in the pathogenesis of the capillary leak syndrome. Finally we have hinted at a role for prophylactic P188 in the vii setting of LCOS, possibly highlighting the role of membrane repair in recovery after surgery. Isolated heart trials of P188 further support a non-rheological mechanism of action and also lend support to the causal separation of myocardial oedema and dysfunction. The integral membrane protein dysferlin, rather than dystrophin, is relevant in the setting of LCOS in the current era
How Uncertain Are Solar Neutrino Predictions?
Solar neutrino fluxes and sound speeds are calculated using a systematic
reevaluation of nuclear fusion rates. The largest uncertainties are identified
and their effects on the solar neutrino fluxes are estimated.Comment: LaTeX file, 12 pages including three postscript figures. To be
published in Phys. Lett. B. One reference correcte
BBN and the Primordial Abundances
The relic abundances of the light elements synthesized during the first few
minutes of the evolution of the Universe provide unique probes of cosmology and
the building blocks for stellar and galactic chemical evolution, while also
enabling constraints on the baryon (nucleon) density and on models of particle
physics beyond the standard model. Recent WMAP analyses of the CBR temperature
fluctuation spectrum, combined with other, relevant, observational data, has
yielded very tight constraints on the baryon density, permitting a detailed,
quantitative confrontation of the predictions of Big Bang Nucleosynthesis with
the post-BBN abundances inferred from observational data. The current status of
this comparison is presented, with an emphasis on the challenges to astronomy,
astrophysics, particle physics, and cosmology it identifies.Comment: To appear in the Proceedings of the ESO/Arcetri Workshop on "Chemical
Abundances and Mixing in Stars in the Milky Way and its Satellites", eds., L.
Pasquini and S. Randich (Springer-Verlag Series, "ESO Astrophysics Symposia"
Solar Neutrino Constraints on the BBN Production of Li
Using the recent WMAP determination of the baryon-to-photon ratio, 10^{10}
\eta = 6.14 to within a few percent, big bang nucleosynthesis (BBN)
calculations can make relatively accurate predictions of the abundances of the
light element isotopes which can be tested against observational abundance
determinations. At this value of \eta, the Li7 abundance is predicted to be
significantly higher than that observed in low metallicity halo dwarf stars.
Among the possible resolutions to this discrepancy are 1) Li7 depletion in the
atmosphere of stars; 2) systematic errors originating from the choice of
stellar parameters - most notably the surface temperature; and 3) systematic
errors in the nuclear cross sections used in the nucleosynthesis calculations.
Here, we explore the last possibility, and focus on possible systematic errors
in the He3(\alpha,\gamma)Be7 reaction, which is the only important Li7
production channel in BBN. The absolute value of the cross section for this key
reaction is known relatively poorly both experimentally and theoretically. The
agreement between the standard solar model and solar neutrino data thus
provides additional constraints on variations in the cross section (S_{34}).
Using the standard solar model of Bahcall, and recent solar neutrino data, we
can exclude systematic S_{34} variations of the magnitude needed to resolve the
BBN Li7 problem at > 95% CL. Additional laboratory data on
He3(\alpha,\gamma)Be7 will sharpen our understanding of both BBN and solar
neutrinos, particularly if care is taken in determining the absolute cross
section and its uncertainties. Nevertheless, it already seems that this
``nuclear fix'' to the Li7 BBN problem is unlikely; other possible solutions
are briefly discussed.Comment: 21 pages, 3 ps figure
Nucleosynthesis Constraints on a Massive Gravitino in Neutralino Dark Matter Scenarios
The decays of massive gravitinos into neutralino dark matter particles and
Standard Model secondaries during or after Big-Bang nucleosynthesis (BBN) may
alter the primordial light-element abundances. We present here details of a new
suite of codes for evaluating such effects, including a new treatment based on
PYTHIA of the evolution of showers induced by hadronic decays of massive,
unstable particles such as a gravitino. We also develop an analytical treatment
of non-thermal hadron propagation in the early universe, and use this to derive
analytical estimates for light-element production and in turn on decaying
particle lifetimes and abundances. We then consider specifically the case of an
unstable massive gravitino within the constrained minimal supersymmetric
extension of the Standard Model (CMSSM). We present upper limits on its
possible primordial abundance before decay for different possible gravitino
masses, with CMSSM parameters along strips where the lightest neutralino
provides all the astrophysical cold dark matter density. We do not find any
CMSSM solution to the cosmological Li7 problem for small m_{3/2}. Discounting
this, for m_{1/2} ~ 500 GeV and tan beta = 10 the other light-element
abundances impose an upper limit m_{3/2} n_{3/2}/n_\gamma < 3 \times 10^{-12}
GeV to < 2 \times 10^{-13} GeV for m_{3/2} = 250 GeV to 1 TeV, which is similar
in both the coannihilation and focus-point strips and somewhat weaker for tan
beta = 50, particularly for larger m_{1/2}. The constraints also weaken in
general for larger m_{3/2}, and for m_{3/2} > 3 TeV we find a narrow range of
m_{3/2} n_{3/2}/n_\gamma, at values which increase with m_{3/2}, where the Li7
abundance is marginally compatible with the other light-element abundances.Comment: 74 pages, 40 Figure
Inhomogeneous Big Bang Nucleosynthesis and Mutual Ion Diffusion
We present a study of inhomogeneous big bang nucleosynthesis with emphasis on
transport phenomena. We combine a hydrodynamic treatment to a nuclear reaction
network and compute the light element abundances for a range of inhomogeneity
parameters. We find that shortly after annihilation of electron-positron pairs,
Thomson scattering on background photons prevents the diffusion of the
remaining electrons. Protons and multiply charged ions then tend to diffuse
into opposite directions so that no net charge is carried. Ions with Z>1 get
enriched in the overdense regions, while protons diffuse out into regions of
lower density. This leads to a second burst of nucleosynthesis in the overdense
regions at T<20 keV, leading to enhanched destruction of deuterium and lithium.
We find a region in the parameter space at 2.1E-10<eta<5.2E-10 where
constraints
7Li/H<10^{-9.7} and D/H<10^{-4.4} are satisfied simultaneously.Comment: 9 pages, minor changes to match the PRD versio
SuperWIMP Dark Matter Signals from the Early Universe
Cold dark matter may be made of superweakly-interacting massive particles,
superWIMPs, that naturally inherit the desired relic density from late decays
of metastable WIMPs. Well-motivated examples are weak-scale gravitinos in
supergravity and Kaluza-Klein gravitons from extra dimensions. These particles
are impossible to detect in all dark matter experiments. We find, however, that
superWIMP dark matter may be discovered through cosmological signatures from
the early universe. In particular, superWIMP dark matter has observable
consequences for Big Bang nucleosynthesis and the cosmic microwave background
(CMB), and may explain the observed underabundance of 7Li without upsetting the
concordance between deuterium and CMB baryometers. We discuss implications for
future probes of CMB black body distortions and collider searches for new
particles. In the course of this study, we also present a model-independent
analysis of entropy production from late-decaying particles in light of WMAP
data.Comment: 19 pages, 5 figures, typos correcte
Pre-main-sequence Lithium Depletion
In this review I briefly discuss the theory of pre-main-sequence (PMS) Li
depletion in low-mass (0.075<M<1.2 Msun) stars and highlight those uncertain
parameters which lead to substantial differences in model predictions. I then
summarise observations of PMS stars in very young open clusters, clusters that
have just reached the ZAMS and briefly highlight recent developments in the
observation of Li in very low-mass PMS stars.Comment: 8 pages, invited review at "Chemical abundances and mixing in stars
in the Milky Way and its satellites", eds. L. Pasquini, S. Randich. ESO
Astrophysics Symposium (Springer-Verlag
Primordial nucleosynthesis with a varying fine structure constant: An improved estimate
We compute primordial light-element abundances for cases with fine structure
constant alpha different from the present value, including many sources of
alpha dependence neglected in previous calculations. Specifically, we consider
contributions arising from Coulomb barrier penetration, photon coupling to
nuclear currents, and the electromagnetic components of nuclear masses. We find
the primordial abundances to depend more weakly on alpha than previously
estimated, by up to a factor of 2 in the case of ^7Li. We discuss the
constraints on variations in alpha from the individual abundance measurements
and the uncertainties affecting these constraints. While the present best
measurements of primordial D/H, ^4He/H, and ^7Li/H may be reconciled pairwise
by adjusting alpha and the universal baryon density, no value of alpha allows
all three to be accommodated simultaneously without consideration of systematic
error. The combination of measured abundances with observations of acoustic
peaks in the cosmic microwave background favors no change in alpha within the
uncertainties.Comment: Phys. Rev. D accepted version; minor changes in response to refere
Physics of rotation in stellar models
In these lecture notes, we present the equations presently used in stellar
interior models in order to compute the effects of axial rotation. We discuss
the hypotheses made. We suggest that the effects of rotation might play a key
role at low metallicity.Comment: 32 pages, 7 figures, lectures, CNRS school, will be published by
Springe