Effect of environmental temperature during the of brooding period on growing period of pullets viscera and tibia

ArticlePoultry production in subtropical and tropical regions faces many problems, one of which is the high air temperature causing thermal stress, particularly dangerous in high-producing birds. Thus, the negative effects caused by heat stress (HS) must be managed. The objective of this study was to evaluate the effects of four different levels of HS in viscera and tibia of pullets. A total of 648 chicks (Lohmann LSL Lite) were used in this study in two different phases. The pre-experimental phase (PEP) was from day 1 through 6 weeks of age. The birds were reared with three different environmental temperatures: thermal comfort, hot and cold. The experimental phase (EP) was conducted from the 7th to the 17th week. Pullets from each thermal environment of the PEP were submitted to: 20 °C, 25 °C, 30 °C, 35 °C. At the end of the 17th week of age 120 pullets were euthanatized and the organs, heart, liver, spleen and gizzard were weighed, as also their tibias. Effects of PEP, and its interaction with EP, were not significant (P < 0.05) for viscera and tibia weight. However, a significant increase (P < 0.05) in heart weight with the decrease of the environmental temperature was observed, being the pullets subject to 20ºC and 25 °C with the heaviest weights. For the liver, pullets subject to the 35 °C had the lowest weight and were different (P < 0.05) from the other three treatments. For gizzard, the difference (P < 0.05) was between the treatments 20ºC and 35 °C. These results indicate that brooding temperatures tested during the first 6 weeks of life did not affect the viscera and bone weight during the growing phase

Partial characterization and anticoagulant activity of a heterofucan from the brown seaweed Padina gymnospora

The brown algae Padina gymnospora contain different fucans. Powdered algae were submitted to proteolysis with the proteolytic enzyme maxataze. The first extract of the algae was constituted of polysaccharides contaminated with lipids, phenols, etc. Fractionation of the fucans with increasing concentrations of acetone produced fractions with different proportions of fucose, xylose, uronic acid, galactose, and sulfate. One of the fractions, precipitated with 50% acetone (v/v), contained an 18-kDa heterofucan (PF1), which was further purified by gel-permeation chromatography on Sephadex G-75 using 0.2 M acetic acid as eluent and characterized by agarose gel electrophoresis in 0.05 M 1,3 diaminopropane/acetate buffer at pH 9.0, methylation and nuclear magnetic resonance spectroscopy. Structural analysis indicates that this fucan has a central core consisting mainly of 3-ß-D-glucuronic acid 1-> or 4-ß-D-glucuronic acid 1 ->, substituted at C-2 with alpha-L-fucose or ß-D-xylose. Sulfate groups were only detected at C-3 of 4-alpha-L-fucose 1-> units. The anticoagulant activity of the PF1 (only 2.5-fold lesser than low molecular weight heparin) estimated by activated partial thromboplastin time was completely abolished upon desulfation by solvolysis in dimethyl sulfoxide, indicating that 3-O-sulfation at C-3 of 4-alpha-L-fucose 1-> units is responsible for the anticoagulant activity of the polymer.Universidade Federal do Rio Grande do Norte Departamento de Bioquímica Laboratório de GlicobiologiaUniversidade Federal do Rio Grande do Norte Departamento de Bioquímica Laboratório de Biotecnologia de Polímeros NaturaisUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de BioquímicaUNIFESP, EPM, Depto. de BioquímicaSciEL