Sleeping sites of woolly mouse opossum Micoureus demerarae (Thomas) (Didelphimorphia, Didelphidae) in the Atlantic Forest of south-eastern Brazil

Micoureus demerarae (Thomas, 1905) is a medium-sized marsupial, around 130 g, with a nocturnal habit and insectivorous-omnivorous diet. From August 2001 to July 2002, seven individuals, three males and four females, were monitored with radio-telemetry in Reserva Biológica União, state of Rio de Janeiro, Brazil, aiming to investigate and describe the sleeping sites used by this marsupial. Fifty eight sleeping sites were located, most of which (70,7%) in palm trees Astrocaryum aculeatissimum (Schott) Burret, and the remaining in other tree species (29,3%), a significant difference (chi2 test; p < 0.005). The preference for this palm tree was not different between sexes (chi2 test; p = 0.920). It was possible to locate the exact place where the animal was hiding in 31 sleeping sites (53.4% of total) in palm trees the animals were always in the junction point of petioles and tree trunks, at an average height of 4.66 ± 1.36 m, while in the remaining tree species, seven individuals were in liana tangles and two in tree holes, at an average height of 10.67 ± 2.75 m. This height difference was significant (Mann Whitney test; p < 0.001). Results indicate that palm trees are important resources for M. demerarae. The observed preference for A. aculeatissimum is probably due to higher protection against predators made by the numerous spines of this palm tree species.<br>Micoureus demerarae (Thomas, 1905) é um marsupial de tamanho médio, cerca de 130 g, de hábito noturno e arborícola e dieta insetívora-onívora. No período de agosto de 2001 a julho de 2002, sete indivíduos, três machos e quatro fêmeas, foram acompanhados, através de rádio-telemetria, na Reserva Biológica União, Rio de Janeiro, com o objetivo de investigar e descrever os abrigos utilizados por essa espécie de marsupial. Foram localizados 58 abrigos, a maioria dos quais (70,7%) em palmeiras Iri Astrocaryum aculeatissimum (Schott) Burret e o restante em outras espécies de árvores (29,3%), uma diferença significativa (teste chi2; p < 0,005). Esta preferência por palmeiras não foi significativamente diferente entre os sexos (teste chi2; p = 0,920). Em 31 abrigos (53,4% do total) o local exato onde o animal se encontrava pode ser localizado: nas palmeiras os animais sempre estavam alojados no local de inserção dos pecíolos junto ao tronco e a uma altura média de 4,66 ± 1,36 m, enquanto nas demais espécies de árvores, sete animais estavam em emaranhados de cipós e dois em ocos, a uma altura média de 10,67 ± 2,75 m. Esta diferença de altura entre abrigos em iris e não iris foi significativa (teste Mann Whitney; p < 0,001). Os resultados indicam que as palmeiras iris são um importante recurso para M. demerarae. A preferência por A. aculeatissimum se deve provavelmente à maior proteção contra predadores exercida pelos numerosos espinhos presentes nesta espécie

Pesquisa de aglutininas anti Brucella canis em soros humanos na cidade de São Paulo, Brasil<a href="#back"></a> Research on agglutinins for Brucella canis in human sera in the city of S. Paulo, Brazil

De 330 soros humanos examinados pela prova de soroaglutinação lenta em tubos, 4(1,21%) apresentaram aglutininas anti Brucella canis em diluição 1:100 (1 reagente com título 100, 2 reagentes com título 200 e 1 reagente com título 400).<br>Of the 330 human sera tested by tube agglutination test, 4 (1.21%) were positive for Brucella canis antibodies with tilers 1:100 or higher (1 reagent with titer of 1:100, 2 reagents with titer of 1:200, and 1 reagent with tiler of 1:400)

Intravenous tolerization with type II collagen induces interleukin-4-and interleukin-10-producing CD4+ T cells

Intravenous (i.v.) administration of type II collagen (CII) is an effective way to induce tolerance and suppress disease in the collagen-induced arthritis (CIA) model. In this study, we demonstrated that a single i.v. dose of CII (as low as 0·1 mg/mouse) completely prevented the development of CIA. This suppression was accompanied by decreases in levels of antibody specific for the immunogen, bovine CII and autoantigen, mouse CII. Splenocytes obtained from CII-tolerized mice and stimulated with CII in vitro produced predominantly the T helper 2 (Th2)-type cytokines interleukin-4 (IL-4) and interleukin-10 (IL-10). In contrast, cells obtained from mice immunized with CII produced predominantly interferon-γ (IFN-γ). Two-colour flow cytometric analysis of cytokine expression and T-cell phenotype demonstrated that CD4+ cells and not CD8+ or γδ+ cells were the predominant regulatory cells producing IL-4 and IL-10. Transgenic mice bearing a T-cell receptor (TCR) specific for CII had a greater increase in the number of IL-4-secreting CD4+ cells, as well as a marked increase of IL-4 in culture supernatants. This cytokine was produced by transgene-bearing T cells. Elucidation of mechanisms for the induction of tolerance in mature T cells is an important line of study in autoimmune models because of the potential application for treating organ-specific autoimmune disease