Action and Function of ASB Proteins in Compartment Size Regulation

One of the most important and defining processes during development is the pattern formation of the various compartments in embryos. In an effort to discover the participants involved in regulating compartment size, we identified, in Danio rerio (zebrafish) embryos, the ankyrin repeat and SOCS box-containing protein 11 (d-asb11) gene. We first showed that d-Asb11 is a key mediator of Delta-Notch Signaling, acting at the level of DeltaA ubiquitylation, important in fine-tuning the lateral inhibition gradients between DeltaA and Notch. We, then, isolated a zebrafish having a germline deletion of the d-Asb11 cullin box subdomain and showed that this deletion resulted in loss of d-Asb11 activity. As a consequence, the animals were defective for Notch signaling and proper cell fate specification within the neurogenic regions of zebrafish embryos. We also provided evidence that d-Asb11 is important in maintaining myogenic proliferation in the stem cell compartment of zebrafish embryos and muscle regenerative responses in adult animals. This finding is supported by the highly specific d-Asb11 expression found in proliferating satellite cells in zebrafish muscle. In addition, we have applied immunoaffinity chromatograpy followed by tandem mass spectrometry to identify human ASB11 interacting proteins. The data confirmed the role of ASB11 as a subs

SEMEAR Project: Planting Seeds for a Better and More Virtuous World

This capstone presents the SEMEAR project. The SEMEAR project is the main initiative of the Brazilian Coaching Society Foundation. The project aims to stimulate and support flourishing in Brazil through positive coaching programs in three major areas: social, educational, and corporative. A set of interventions designed to enhance well-being, facilitate healthy relationships, promote flow and engagement, stimulate social contribution, as well as other aspects that can help people flourish, will be proposed. The project will explore how to use coaching, positive psychology and philanthropy to develop virtues, promote human flourishing and generate people’s well-being in families, schools, companies and communities through change agents such as coaches, parents, educators and leaders. Therefore, the project plans to engage and prepare 1,000,000 positive coaches who will directly or indirectly impact 50,000,000 people by 2050, contributing to the flourishing of 25% of the Brazilian population

Vascular Parkinsonism: Analysis Of Seven Cases

INTRODUCTION: Neuroimaging studies of elderly individuals reveal alterations in the white matter that are incompatible with the patient's parkinsonism, mistakenly classified as vascular parkinsonism (VP). METHOD: This study was conducted on a population composed of 20 patients with Parkinson's disease (PD) whose neuroimaging exams revealed vascular alterations in the white matter and seven patients with VP in order to compare diagnostic criteria. RESULTS: Age at disease onset of patients with PD was 55±12 years and patients with VP it was 62±13 years. Twelve patients with PD and five patients with VP presented arterial hypertension; three patients with VP and two patients with PD presented gait impairment; all patients with VP presented rigidity and bradykinesia, six of them presented resting tremor; 19 patients with PD presented tremor and 19 of them presented rigidity, while 17 presented bradykinesia. When the symptoms and evolution of both diseases were compared, the vascular alterations in the white matter were considered unspecific. CONCLUSION: Since clinical symptoms are unspecific, a differential diagnosis requires neuroimaging, good response to levodopa and clinical evolution. © 2006 Associação Arquivos de Neuro-Psiquiatria.6403:00:00Critchley, M., Arteriosclerotic parkinsonism (1929) Brain, 52, pp. 23-83Winikates, J., Jankovic, J., Clinical correlates of vascular parkinsonism (1999) Arch Neurol, 56, pp. 98-102Demirkiran, M., Bozdemir, H., Sarica, Y., Vascular parkinsonism: A distinct, heterogeneous clinical entity (2001) Acta Neurol Scand, 104, pp. 63-67Thanvi, B., Lo, N., Robinson, T., Vascular parkinsonism: An important cause of parkinsonism in older people (2005) Age Ageing, 34, pp. 114-119Zijlmans, J.C.M., Thijssen, H.O.M., Vogels, O.J.M., MRI in patients with suspected vascular parkinsonism (1995) Neurology, 45, pp. 2183-2188Bennet, D.A., Wilson, R.S., Gilley, D.W., Fox, J.H., Clinical diagnosis of Binswanger disease (1990) J Neurol Neurosurg Psychiatry, 53, pp. 961-965Hurtig, H.I., Vascular parkinsonism (1993) Parkinsonian Syndromes, pp. 81-83. , Stern MB, Koller WC (eds). New York: Marcel DekkerBaldereschi, M., Di Carlo, A., Rocca, W.A., Parkinson's disease and parkinsonism in a longitudinal study: Two-fold higher incidence in men (2000) Neurology, 55, pp. 1358-1363Foltynie, T., Barker, R., Brayne, C., Vascular parkinsonism: A review of the precision and frequency of diagnosis (2002) Neuroepidemiology, 21, pp. 1-7Benito-Leon, J., Bernejo-Pareja, F., Moralez-Gonzalez, J.M., Incidence of Parkinson's disease and parkinsonism in three elderly populations of central Spain (2004) Neurology, 62, pp. 734-741Bower, J.H., Dickson, D.W., Taylor, L., Maraganore, D.M., Rocca, W.A., Clinical correlates of the pathology underlyng parkinsonism: A population perpective (2002) Mov Disord, 17, pp. 910-916Zijlmans, J.C.M., Daniel, Se., Hughes, A.J., Révész, T., Lees, A.J., Clinicopathological investigation of vascular parkinsonism, including clinical criteria for diagnosis (2004) Mov Disord, 19, pp. 630-640Fazekas, F., Niederkorn, K., Schmidt, R., White matter signal abnormalities in normal individuals: Correlation with carotid ultrasound, cerebral blood flow measurements, and cerebrovascular disease risk factors (1988) Stroke, 19, pp. 1285-1288Larsen, J.P., Dupont, E., Tandberg, E., Clinical diagnosis of Parkinson's disease: Proposal of diagnostic subgroups classified at different levels of confidence (1994) Acta Neurol Scand, 89, pp. 242-251Elbaz, A., Bower, J.H., Maraganore, D.M., Risk tables for parkinsonism and Parkinson's disease (2002) J Clin Epidemiol, 55, pp. 25-31(1994) Diagnostic and Statistical Manual of Mental Disorders. Fourth Edition, , American Psychiatric Association Washington, DC: American Psychiatric AssociationSibon, I., Fenelon, G., Quinn, N.P., Tison, F., Vascular parkinsonism (2004) J Neurol, 251, pp. 513-524Zijlmans, J.C.M., Katzenschlager, R., Daniel, S.E., Lees, A.J.L., The L-dopa response in vascular parkinsonism (2004) J Neurol Neurosurg Psychiatry, 75, pp. 545-547Piccini, P., Pavese, N., Canapichi, R., White matter hiperintensities in Parkinson's disease (1995) Arch Neurol, 52, pp. 191-194Murrow, R.W., Schweiger, G.D., Kepes, J.J., Koller, W.C., Parkinsonism due to basal lacunar state: A clinicopathological correlation (1990) Neurology, 40, pp. 897-90

Quality Of Life In Patients With Parkinson's Disease And Their Caregivers' Stress Levels

Introduction. The quality of life in patients with Parkinson's disease (PD) is related to the disease level, which also indicates the caregiver's quality of life. Methods. This study comprised 43 patients with PD (31 male) and their respective caregivers (35 female) in order to evaluate if these disease symptoms may change their quality of life. Results. There was a statistically significant correlation between quality of life and the disease level and caregiver stress. The higher the score of disease level, the worse the patients and caregivers' quality of life. Conclusion. The disease control improves the patients' quality of life and, consequently, the caregivers' quality of life.162113117Quagliato, L.B., Viana, M.A., Quagliato, E.M.A.B., Simis, S., Alterações do olfato na doença de Parkinson (2007) Arq Neuropsiq, 65 (3 A), pp. 647-652Anderson, R., Bury, M., (1988) Living with chronic illness: The experience of patients and their families, pp. 88-116. , London: Allen & UnwinJacoby, A., Backer, G.A., Smith, D.F., Dewey, M.E., Chadwick, D.W., Measuring the impact of epilepsy: The development of a novel scale (1993) Epi Res, 16, pp. 83-88Price, B., Illness cares: The chronic illness experience (1996) J Adv Nurs, 24, pp. 275-279Lima, S.S.P., Quagliato, E.M.A.B., Cagliari, L.C., Souza, E.A.P., (1997) Linguagem e isolamento social no Mal de Parkinson, 1 (2), pp. 5-13. , Rev Soc Bras FonoaudiolGray, A., McNamara, I., Aziz, T., Gregory, R., Bain, P., Wilson, J., Quality of life outcomes following surgical treatment of Parkinson's disease (2002) Mov Disord, 17 (1), pp. 68-75Silva EG, Viana MA, Quagliato EMAB. Pain in Parkinson's disease: analysis of 50 cases in a clinic of movement disorders. Arq Neuropsiquiatr 2008;66(1):26-9Souza, E.A.P., Guerreiro, M.M., (1996) Qualidade de vida, pp. 191-199. , Guerreiro CAM, Guerreiro MM. Epilepsia. São Paulo: LemosSouza, E.A.P., Questionário de qualidade de vida na epilepsia: Resultados preliminares (2001) Arq Neuropsiquiatr, 59 (3 A), pp. 540-544Martínez-Martín, P., An introduction to the concept of "Quality of Life in Parkinson's disease (1998) J Neurol, 245 (S1), pp. S2-S6De Boer, A.G.E.M., Sprangers, M.A.G., Speelman, H.D., De Haes, H.C.J.M., Predictors of Health Care Use in patients with Parkinson's disease: A longitudinal study (1999) Mov Disord, 14 (5), pp. 772-779Salgado, P.C.B., Souza, E.A.P., Qualidade de vida em epilepsia e percepção de controle de crises (2001) Arq Neuropsiquiatr, 59 (3 A), pp. 537-540Cardoso, F., Principais perguntas sobre parkinsonismo e doença de Parkinson. Beija-flor-São Paulo (2000), 47, pp. 6-8Schrag, A., Jahanshahi, M., Quinn, N., How does Parkinson's disease affect quality of life? A comparison with quality of life in the general population (2000) Mov Disord, 15 (6), pp. 1112-1118Hughes, A.J., Daniel, S.E., Kilford, L., Lees, A.J., Accuracy of clinical diagnosis of idiopathic Parkinson's disease: A clinicopathological study of 100 cases (1992) J Neurol Neurosurg Psychiatr, 55 (3), pp. 181-184Fahn S, Elton RL, and members of the UPDRS Development Committee. Unified Parkinson's disease rating scale. In: Fahn S, Marsden CD, Goldstein M, Calne DB (eds). Recent Development in Parkinson's disease. New Jersey: Florham Park, 1987,153-63Jenkinson, C., Fitzpatrick, R., Peto, V., Greenhall, R., Hyman, N., The Parkinson's Disease Questionnaire (PDQ-39): Development and validation of Parkinson's disease summary index score (1997) Age Ageing, 26 (5), pp. 353-357McRae C, Sherry P, Roper K. Stress in family functioning among caregiver of persons in Parkinson's disease. Parkinson Rel Dis 1999;5:69-75Zhang, Z., Roman, G.C., Worldwide occurrence of Parkinson's disease: An updated review (1993) Neuroepidemiol, 12, pp. 195-208Lee, K.S., Merriman, A., Owen, A., Chew, B., Tan, T.C., The medical, social and functional profile of Parkinson's disease patients (1994) Singap Med J, 35 (3), pp. 265-268Happe, S., Berger, K., The association between caregiver burden and sleep disturbances in partners of patients with Parkinson's disease (2002) Age and Aging, 31, pp. 349-354Silva, E.G., Viana, M.A., Quagliato, E.M.A.B., Diagnóstico de síndromes parkinsonianas em uma clínica brasileira de distúrbios do movimento. (2005) Rev Neurocienc, 13 (4), pp. 173-177Hankin, B.L., Abramson, L.Y., Development of gender differences in depression: An elaborate cognitive vulnerability-transactional stress theory (2001) Psychol Bull, 127, pp. 773-796Pasetti, C., Ferrario, S.R., Fornara, R., Picco, D., Foglia, C., Galli, J., Caregiving and Parkinson's disease (2003) Neurol Sci, 24, pp. 203-204De Boer, A.G.E.M., Wijker, W., Speelman, J.D., De Haes, J.C.J.M., Quality of life in patients with Parkinson's disease: Development of a questionnaire (1996) J Neurol Neurosur Psychiatr, 61, pp. 70-74Hobson, P., Holden, A., Meara, J., Measuring the impact of Parkinson's disease with Parkinson's disease Quality of Life Questionnaire (1999) Age and Aging, 28, pp. 341-346Mc Rae, C., O'Brien, C., Treed, C., Quality of life among persons receiving neural implants surgery for Parkinson's disease (1996) Mov Disord, 11, pp. 605-60

Novos Isolados De Trichoderma Antagônicos A Sclerotinia Sclerotiorum

Forty-nine isolates of Trichoderma from the Brazilian Midwest were evaluated for their antagonistic activity in vitro against Sclerotinia sclerotiorum (causal agent of white mold), which were then identified based on their nuclear ribosomal ITS sequences. Paired culture tests showed that all isolates exhibited some antagonism, with a maximum of 77% mycelial inhibition and complete inhibition of sclerotia production. Two isolates were found to be the most promising biocontrol agents, considering both antagonistic parameters (CEN1253 - T. koningiopsis and CEN1265 - T. brevicompactum). Five different species were identified: T. harzianum (23), T. spirale (9), T. koningiopsis (8), T. brevicompactum (7) and T. asperellum (2). These isolates are stored in the Embrapa Fungi Collection for Biological Control and the information obtained in the experiments will be incorporated into the database of biological assets within the genetic resources information system (Allele) and be made available for further studies. © 2016, Universidade Estadual de Campinas UNICAMP. All rights reserved.16

Nanosieves Fabricated By Interference Lithography And Electroforming

Self-sustaining Nickel membranes with periodic and regular distribution of pores, in the scale of hundred of nanometers, were produced by interference lithography and electroplating. The process consists in the recording of submicrometric 2D periodic photoresist columns, on a metal-coated glass substrate, using the double exposure of an interference fringe pattern. As the photoresist is a good electrical isolator, when the sample is immersed in a Ni electroplating bath, the array of photoresist columns impedes the Nickel deposition in the patterned areas. A nickel film is then growth among the photoresist columns with a thickness up to 80 % of the height of the columns. In order to release the submicrometric membrane from the substrate, a thick hexagonal Nickel sustaining structure is electroformed, using conventional photolithography. The dimensions of the sustaining structure can be adapted in order to fulfill the pressure requirements of the filtration system. The good uniformity of the pore sizes as well as the smooth of the surface make such devices very interesting for separation of particles by size in filtration systems.6037Sard Ghayeni, S.B., Beatson, P.J., Fane, A.J., Schneider, R.P., Bacterial passage through microfiltration membranes in wastewater applications (1999) J. Membr. Sci., 153, pp. 71-82(2002) Life Science CatalogueCalvo, J.I., Hernandez, A., Pradanos, P., Martinez, L., Bowen, W.R., Pore size distribution in microporous membranes, II bulk characterization of track-etched filters by air porometry and mercury prosimetry (1995) Journal of Colloid and Interface Science, 176, pp. 467-478Han, K., Xu, W., Ruiz, A., Ruchhoeft, P., Chellam, S., Fabrication and characterization of polymeric microfiltration membranes using aperture array lithography (2005) J. Membr. Sci., 249, pp. 193-206Kuiper, S., Van Wolferen, H., Van Rijn, C., Nijdam, W., Krijnem, G., Elwenspoek, M., Fabrication of microsieves with sub-micron pore size by laser interference lithography (2001) Journal, of Micromehcanics and Microengineering, 11, pp. 33-37Van Rijn, C.J.M., Veldhuis, G.J., Kuiper, S., Nanosieves with microsystem tecnology for microfiltration applications (1998) Nanotechnology, 9, pp. 343-345Kuiper, S., Boer, M.D., Van Rijn, C., Nijdarh, W., Krijnen, G., Elwenspoek, M., Wet and dry etching techniques for the release of sub-micrometre perforated membranes (2000) Journal of Micromechanics and Microengineering, 10, pp. 171-174Gutierrez-Rivera, L.E., De Carvalho, E.J., Suva, M.A., Cescato, L., Metallic submicrometric sieves fabricated by interferometric litography and electroforming (2005) Journal of Micromechanics and Microengineering, 15, pp. 1932-1937Griffiths, S.K., Nilson, R.H., Hruby, J.M., (1996) Modeling Electrodeposition for LIGA Microdevice Fabrication, , http://www.ca.sandia.gov/liga/process_archives.html, Sandia National Laboratories, Livermore, CaliforniaFrejlich, J., Cescato, L., Mendes, G.F., Analysis of an active stabilization system for an holographic setup (1998) Appl. Opt., 27, pp. 1967-1976Zaidi, H.S., Brueck, S.R.J., Multiple-exposure interferometric lithography (1993) J. Vac. Sci. Technol. B, 11, pp. 658-666Mello, B.A., Costa, I.F., Lima, C.R.A., Cescato, L., Developed profile of holographically exposed photoresist gratings (1995) Applied Optics, 34, pp. 597-603Mack, C.A., Development of positive photoresists (1987) J. Electrochem. Soc., 134, pp. 148-152Spiro, P., (1971) Electroforming: A Comprehensive Survey of Theory, Practice and Commercial Applications2th Edition, , Robert Draper LTDKuiper, S., Van Rijn, C.J., Nijdam, W., Elwenspoek, M.C., Development and applications of very high flux microfiltration membranes (1998) Journal of Membrane Science, 150, pp. 1-8www.mediacy.co

Pro-oxidant Activity And Genotoxicity Of The Astronium Fraxinifolium Using Wing Smart And Allium Cepa Test

Astronium fraxinifolium is an arboreal species found throughout the Brazilian Cerrado region and used in folk medicine as antimicrobial, anti-hemorrhagic and healing. Pro-oxidant activity of extracts of fraxinifolium rough Relative Electrophoresis Mobility (REM) of Bovine Serum Albumin (BSA) protein in presence of the extract and Cu2+ and also the genotoxic potential through Somatic Mutation and Recombination Test (SMART) and Allium cepa tests has been investigated during this study. In the REM, the extracts acting exclusively on BSA resulted in a band formation with higher molecular weight than BSA, probably due to oxidative action of the extracts. In presence of Cu2+and extracts occurred protein fragmentation due to Cu2+ oxidative action potentiated by the extracts. In SMART test the frequency of mutant spots increases with the increased concentration of extract A. fraxinifolium (50 mg mL-1= 1.40 and 100 mg mL-1 = 2.66). The HB cross shows a decrease in the total of mutant spots frequency for the different treatments (50 mg mL-1 =6.60 and 100 mg mL-1 = 3.25). Evaluation of A cutest demonstrated the following results (concentration extract = chromosomal abnormalities) 1 mg mL-1 = 85,10 mg L-1 = 61 50 mg mL-1 = 53,100 mg mL-1 =33 and MMS 10 mg L-1 = 50. Genotoxic and cytotoxic actions can be explained by the actions of tannins present in its composition. Butthere may be other substances that also act for such results. The genotoxicity in medicinal plants contributes to therapeutic safety warning that although the use of medicinal plants is an inexpensive and non-aggressive method it can cause harmful effects if used incorrectly. © 2016 Rafael Rozolen Teixeria Zafred et al.10427628

Uso Do 1-mcp E Atmosfera Modificada Na Pós-colheita De Atemoia 'gefner'

The scarcity of technologies related to the post-harvest physiology of this species is one of the barriers to commercialization of atemoya over long distances, aggravated by perishability presented by them. Thus, the objective of this study was to prolong the shelf life of atemoya fruits using 1-methylcyclopropene (1-MCP) and modified atmosphere by low-density polyethylene (LDP), associated with refrigeration. The experiment was conducted in a completely randomized design in a split-plot, with plots in the four doses of 1-MCP (0, 200, 400 and 600 ?L L-1), and in the 5 subplots evaluation periods after harvest in the range of 5 days, with four replicate. Atemoyas were harvested at physiological maturity then treated with doses of 1-MCP for 8 h at room temperature. Soon after, the fruits were placed in polystyrene trays. Some remained unpacked and others were packed with a plastic film of LDP 16 μm. The use of modified atmosphere and 1-MCP alone or combined, were effective in delaying the ripening of fruits, allowing the preservation of its chemistry quality. In the fruits treated with 1-MCP was no delay in the accumulation of soluble solids and starch degradation.112677

Leonardites rich in humic and fulvic acids had little effect on tissue elemental composition and dry matter yield in pot-grown olive cuttings

The use of humic substances in agriculture has increased in recent years, and leonardite has been an important raw material in the manufacture of commercial products rich in humic and fulvic acids. Leonardite-based products have been used to improve soil properties and to help plants cope with abiotic and biotic stresses. In this study, the effects of two commercial leonardites and an organic compost, in addition to a control treatment, were assessed for pot-grown olive plants over a period of fourteen months on soil properties, tissue elemental composition and dry matter yield (DMY). Three organic amendments were applied at single and double rates of that set by the manufacturer. The study was arranged in two experiments: one containing the seven treatments mentioned above and the other containing the same treatments supplemented with mineral nitrogen (N), phosphorus (P) and potassium (K) fertilization. Overall, organic compost increased soil organic carbon by ~8% over the control. In the experiment without NPK supplementation, N concentrations in shoots and P in roots were the highest for the compost application (leaf N 12% and root P 32% higher than in the control), while in the experiment with NPK supplementation, no significant differences were observed between treatments. Total DMY was ~10% higher in the set of treatments with NPK in comparison to treatments without NPK. Leonardites did not affect significantly any measured variables in comparison to the control. In this study, a good management of the majority of environmental variables affecting plant growth may have reduced the possibility of obtaining a positive effect on plant nutritional status and growth from the use of commercial leonardites. The leonardites seemed to have caused a slight effect on biological N immobilization. This is not necessarily an advantage or a drawback; it is rather a feature that must be understood to help farmers make better use of these products.This research was funded by the Foundation for Science and Technology (FCT, Portugal) and FEDER under Programme PT2020 for financial support to CIMO (UIDB/00690/2020). The research was integrated in the activities of the operational group “Novas práticas em olivais de sequeiro: estratégias de mitigação e adaptação às alterações climáticas”, funded by PT2020 and EAFRD (European Agricultural Fund for Rural Development).info:eu-repo/semantics/publishedVersio