Conceptual understanding in genetics

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A genomic scan for selection reveals candidates for genes involved in the evolution of cultivated sunflower (Helianthus annuus)

Genomic scans for selection are a useful tool for identifying genes underlying phenotypic transitions. In this article, we describe the results of a genome scan designed to identify candidates for genes targeted by selection during the evolution of cultivated sunflower. This work involved screening 492 loci derived from ESTs on a large panel of wild, primitive (i.e., landrace), and improved sunflower (Helianthus annuus) lines. This sampling strategy allowed us to identify candidates for selectively important genes and investigate the likely timing of selection. Thirty-six genes showed evidence of selection during either domestication or improvement based on multiple criteria, and a sequence-based test of selection on a subset of these loci confirmed this result. In view of what is known about the structure of linkage disequilibrium across the sunflower genome, these genes are themselves likely to have been targeted by selection, rather than being merely linked to the actual targets. While the selection candidates showed a broad range of putative functions, they were enriched for genes involved in amino acid synthesis and protein catabolism. Given that a similar pattern has been detected in maize (Zea mays), this finding suggests that selection on amino acid composition may be a general feature of the evolution of crop plants. In terms of genomic locations, the selection candidates were significantly clustered near quantitative trait loci (QTL) that contribute to phenotypic differences between wild and cultivated sunflower, and specific instances of QTL colocalization provide some clues asto the roles that these genes may have played during sunflower evolution

Microleakage of four different restorative glass ionomer formulations in Class V cavities: Er:YAG laser versus conventional preparation

Objective: To investigate microleakage in class V cavities following restoration with conventional glass-ionomer cements (CGICs) or resin-modified glass-ionomer cements (RMGICs), following Er:YAG laser or conventional preparation. Background Data: The sealing ability of GICs in Er:YAG-lased cavities has not been studied extensively. Methods: Three hundred and twenty class V cavities were assigned to four groups: those in groups A and B were prepared using an Er: YAG laser, and those in groups C and D were conventionally prepared. In groups B and D the surface was additionally conditioned with cavity conditioner. Each group was subdivided according to the GIC used: groups 1 (Fuji II), 2 (Fuji IX), 3 (Fuji II LC) and 4 ( Fuji VIII). After thermocycling, the specimens were immersed in a 2% methylene blue solution, sectioned oro-facially, and analyzed for leakage. The effect of the conditioner was analyzed using a scanning electron microscope (SEM). Results: Significant differences between occlusal and gingival margins were found in all groups (p < 0.05) except B4, D3, and D4. Comparison of preparation methods (groups A-D) revealed significant differences at the occlusal margin in groups 1 and 3, but in all groups at the gingival margin (p < 0.05). Laser preparation without conditioning allowed more leakage (p < 0.05). Comparison of filling materials (groups 1-4) revealed significant differences in groups B and C at the occlusal margin, and in all groups at the gingival margin (p < 0.05). In these groups, laser-prepared cavities (with or without conditioning) restored with Fuji II LC and Fuji VIII showed the least leakage at both margins. Conclusion: RMGICs allowed less microleakage than CGICs. Complete marginal sealing was not achieved and conditioning is recommended