Ising model with spins S=1/2 and 1 on directed and undirected Erd\"os-R\'enyi random graphs

Using Monte Carlo simulations we study the Ising model with spin S=1/2 and 1 on {\it directed} and {\it undirected} Erd\"os-R\'enyi (ER) random graphs, with $z$ neighbors for each spin. In the case with spin S=1/2, the {\it undirected} and {\it directed} ER graphs present a spontaneous magnetization in the universality class of mean field theory, where in both {\it directed} and {\it undirected} ER graphs the model presents a spontaneous magnetization at $p = z/N$ ($z=2, 3, ...,N$), but no spontaneous magnetization at $p = 1/N$ which is the percolation threshold. For both {\it directed} and {\it undirected} ER graphs with spin S=1 we find a first-order phase transition for z=4 and 9 neighbors.Comment: 11 pages, 8 figure

Immunophenotypic characterization of normal blood CD56+lo versus CD56+hi NK‐cell subsets and its impact on the understanding of their tissue distribution and functional properties

Blood Cells Mol Dis. 2001 Jul-Aug;27(4):731-43. Immunophenotypic characterization of normal blood CD56+lo versus CD56+hi NK-cell subsets and its impact on the understanding of their tissue distribution and functional properties. Lima M, Teixeira MA, Queirós ML, Leite M, Santos AH, Justiça B, Orfão A. Service of Clinical Hematology, Unit of Cytometry, Hospital Geral de Santo António, Porto, Portugal. [email protected] Abstract In the present study we have compared the immunophenotypic characteristics of the CD56+lo and CD56+hi NK-cell subsets in a group of normal healthy adults. Our results show that CD56+hi NK-cells display greater light-scatter properties than CD56+lo NK-cells at the same time they have higher levels of CD25 and CD122 IL-2 chains, together with a higher reactivity for HLA-DR and CD45RO and lower levels of CD45RA, supporting that, as opposed to the majority of the CD56+lo population, CD56+hi NK-cells might correspond to a subset of activated circulating NK-lymphocytes. Higher expression of the CD2 and CD7 costimulatory molecules found for the CD56+hi NK-cells would support their greater ability to respond to various stimuli. In addition, CD56+hi NK-cells expressed higher levels of several adhesion molecules such as CD2, CD11c, CD44, CD56, and CD62L compared to CD56+lo NK-cells, supporting a particular ability of these cells to migrate from blood to tissues and/or a potential advantage to form conjugates with target cells. Interestingly, CD56+lo and CD56+hi NK-cells showed a different pattern of expression of killer receptors that might determine different activation requirements for each of these NK-cell subsets. For instance, absence or low levels of CD16 expression might explain the lower antibody-dependent cytotoxicity activity of CD56+hi NK-cells. On the other hand, the virtual absence of expression of the CD158a and NKB1 immunoglobulin-like and the greater reactivity for the CD94 lectin-like killer receptors on CD56+hi in comparison to CD56+lo NK-cells might determine different MHC-class I specificities for both NK-cell subsets, a possibility that deserves further studies to be confirmed. PMID: 11778657 [PubMed - indexed for MEDLINE

Higher spin fields and the problem of cosmological constant

The cosmological evolution of free massless vector or tensor (but not gauge) fields minimally coupled to gravity is analyzed. It is shown that there are some unstable solutions for these fields in De Sitter background. The back reaction of the energy-momentum tensor of such solutions to the original cosmological constant exactly cancels the latter and the expansion regime changes from the exponential to the power law one. In contrast to the adjustment mechanism realized by a scalar field the gravitational coupling constant in this model is time-independent and the resulting cosmology may resemble the realistic one.Comment: 15 pages, Latex twic

Cryopreservation of collared peccary (Pecari tajacu) semen using different freezing curves, straw sizes, and thawing rates

AbstractThe objective of this study was to verify the effect of different freezing curves, straw sizes, and thawing rates on the cryopreservation of collared peccary semen. Twelve ejaculates were obtained from captive adult males by electroejaculation, and evaluated for sperm motility, kinetic rating, viability, morphology, and functional membrane integrity. The ejaculates were diluted in a coconut water extender (ACP-116c) with egg yolk and glycerol, packaged into 0.25mL or 0.50mL plastic straws and cryopreserved in liquid nitrogen following a slow (−10°C/min) or a fast (−40°C/min) freezing curve. After one week, samples were thawed at 37°C/1min or 70°C/8s and evaluated as reported for fresh semen, and also for kinematic parameters (computerized analysis). A significant decrease in sperm motility and kinetic rating was observed after glycerol addition at 5°C and also after thawing for all the treatments (P<0.05). Regarding post-thaw semen variables, no differences were verified between freezing curves when the same straw size and thawing rate were taken as reference (P>0.05). In general, values for sperm characteristics found after thawing at 37°C were better preserved than at 70°C (P<0.05), both in the use of 0.25mL or 0.50mL straws, which were similar for semen packaging (P>0.05). The evaluation of the kinematic parameters of sperm motility confirmed these results at values varying from 20% to 30% motile sperm for the samples thawed at 37°C, and values fewer than 12% motile sperm for samples thawed at 70°C (P<0.05). In conclusion, we recommend the use of a fast freezing curve that reduces the time spent on the cryopreservation of collared peccary semen, which could be packaged both in 0.25mL or 0.50mL straws, but the thawing should be conducted at 37°C/1min