Immunomodulatory properties of mesenchymal stromal cells can vary in genetically modified rats

Mesenchymal Stromal Cells (MSC) have been shown to exhibit immuno-modulatory and regenerative properties at sites of inflammation. In solid organ transplantation (SOT), administration of MSCs might lead to an alleviation of ischemia-reperfusion injury and a reduction of rejection episodes. Previous reports have suggested ‘MSC-preconditioning’ of macrophages to be partly responsible for the beneficial effects. Whether this results from direct cell-cell interactions (e.g., MSC trans-differentiation at sites of damage), or from paracrine mechanisms, remains unclear. Immunosuppressive capacities of MSCs from donors of different age and from genetically modified donor animals, often used for in-vivo experiments, have so far not been investigated. We conducted an in vitro study to compare paracrine effects of supernatants from MSCs extracted from young and old wild-type Wystar-Kyoto rats (WKY-wt), as well as young and old WKY donor rats positive for the expression of green fluorescent protein (WKY-GFP), on bone marrow derived macrophages (BMDM). Expression levels of Mannose receptor 1 (Mrc-1), Tumor necrosis factor α (TNFα), inducible NO synthase (iNos) and Interleukin-10 (IL-10) in BMDMs after treatment with different MSC supernatants were compared by performance of quantitative PCR. We observed different expression patterns of inflammatory markers within BMDMs, depending on age and genotype of origin for MSC supernatants. This must be taken into consideration for preclinical and clinical studies, for which MSCs will be used to treat transplant patients, aiming to mitigate inflammatory and allo-responses

Effects of Er and Zr Additions on the As-Cast Microstructure and on the Solution-Heat-Treatment Response of Innovative Al-Si-Mg-Based Alloys

The microstructure of Al-Si-Mg alloys strongly depends on their chemical composition and the heat treatment they undergo during production. The influence of solution heat treatment (SHT) and the addition of Er and Zr on the microstructure of gravity-cast A356 (Al-7Si-0.4Mg) were examined. The reference as-cast microstructure is characterized by the grain size and morphology of eutectic Si, as well as the morphology, area fraction, and chemical composition of the intermetallic compounds. The morphology of eutectic Si is unstable during SHT; the evolution mechanisms can be described using thermodynamic and kinetic models and have been validated using optical and scanning electron microscope (SEM) micrographs. The effect of high-temperature exposure during SHT, on the other hand, plays a minor role on the quantity and morphology of the intermetallic compounds, as demonstrated by optical and SEM micrographs

An improved rapid sampling microdialysis system for human and porcine organ monitoring in a hospital setting

Online organ monitoring could provide clinicians with critical information regarding organ health prior to transplantation and could aid clinical decision-making. This paper presents the methodology of online microdialysis for real-time monitoring of human organs ex vivo. We describe how rapid sampling microdialysis can be incorporated with organ perfusion machines to create a robust organ monitoring system and demonstrate its use in monitoring human and porcine kidneys as well as human and porcine pancreases. In this paper we also show the potential usefulness of this methodology for evaluating novel interventions in a research setting. The analysis system can be configured either to analyse two analytes in one organ, allowing for ratiometric analysis, or alternatively to monitor one analyte in two organs simultaneously, allowing direct comparison. It was found to be reliable over long monitoring periods in real clinical use. The results clearly show that the analysis system is sensitive to differences between organs and therefore has huge potential as an ex vivo organ monitoring tool

Characterization of localized corrosion of heat treated Er- and Zr-containing A356 alloys in 3.5 wt% NaCl aqueous solution

The influences of Er and Zr additions on the corrosion behavior of A356 (Al-7Si-0.4Mg) alloy are investigated. Electrochemical tests (potentiodynamic polarization and electrochemical impedance spectroscopy), gravimetric analyses, and microstructural investigations demonstrate that 0.3 wt% Er nominal addition is beneficial for the corrosion resistance of A356 alloy, due to a modified eutectic morphology and a low volume fraction of intermetallic compounds. The further addition of about 0.5 wt% Zr, on the other hand, partially reduces the corrosion resistance of Er-containing A356 alloy, possibly due to a higher amount of intermetallic compounds which promote corrosion localization

Unified Access to Heterogeneous Audiovisual Archives

In this paper, an integrated information system is presented that offers enhanced search and retrieval capabilities to users of heterogeneous digital audiovisual (a/v) archives. This innovative system exploits the advances in handlings a/v content and related metadata, as introduced by MPEG-4 and worked out by MPEG-7, to offer advanced services characterized by the tri-fold semantic phrasing of the request (query), unified handling and personalized response. The proposed system is targeting the intelligent extraction of semantic information from a/v and text related data taking into account the nature of the queries that users my issue, and the context determined by user profiles. It also provides a personalization process of the response in order to provide end_users with desired information. From a technical point of view, the FAETHON system plays the role of an intermediate access server residing between the end users and multiple heterogeneous audiovisual archives organized according to the new MPEG standards