Muscle trematode infection of alien cyprinids in the basin of the Middle Ob (Sibiria, Russia)

The book represents proceedings of Sixth International Symposium “Invasion of Alien Species in Holarctic. Borok -VI” (11 Oct. – 15 Oct. 2021, Borok – Uglich, Russia). The wide spectrum of problems related to appearance and spread of invasive plants and animals is discussed. The book may be interested for specialists in many fields, such as limnologists, hydrobiologists, ecologists, botanists, zoologists, geographers, managers of dealing with nature preservation and fisheries

The role of non-commercial cyprinids in maintenance and spread of the opisthorchiasis focus in the middle Ob River basin (Tomsk region, Russia)

The study assessed the role of non-commercial cyprinid species in maintaining the opisthorchiasis focus in the middle Ob River basin, Tomsk region, Russia. The source of O. felineus infection for humans and carnivores is fish of the family Cyprinidae. This is the most numerous family, 14 species live in the middle Ob River basin, which includes 6 commercial species and 8 non-commercial species. This study aimed to investigate the current situation on infestation of non-commercial cyprinids with O. felineus metacercariae and their role in maintaining and spreading the natural focus of opisthorchiasis in the middle Ob River basin. We investigated 4 non-commercial species (tench, sunbleak, common bleak, gudgeon), which are highly abundant in water bodies. Tench, common bleak and gudgeon are objects of amateur fishing. These species are traditionally included in the diet of the local population. Opisthorchis felineus metacercariae were recorded in muscles of all the examined fish species. The identification of metacercariae was confirmed by morphological methods and PCR diagnostics. Tench and sunbleak are the main sources of opisthorchiasis infection in the floodplain lakes of the Ob River basin (the prevalence of tench infection is 89.3% and mean intensity of infection is 11.2 metacercariae per fish, the prevalence of sunbleak infection is 50.9% and the intensity of infection is 4.25 metacercariae per fish). The prevalence of infection in the introduced common bleak from the rivers of the middle Ob River basin is rapidly increasing from 2.4 (2016-2018) to 37.5% (2020-2021), and mean intensity of infection increased from 1 to 4.15. The epizootic state of water bodies in the middle Ob River basin remains unfavorable in relation to opisthorchiasis. Tench, common bleak and sunbleak, along with ide and dace, are the main source of infection for humans and animals, which is evidenced by high infection with Opisthorchis felineus metacercariae in these numerous fish species. They pose the greatest danger of infection of people and animals with opisthorchiasis. These species should be included in the campaign to avoid raw and poorly cooked fish in the diet. In addition, such species as roach, bream and sunbleak also pose the danger of infection with opisthorchiasis, but to a lesser extent

Measurements of production and inelastic cross sections for p+C, p+Be, and p+Al at 60 GeV/c and p+C and p+Be at 120 GeV/c

This paper presents measurements of production cross sections and inelastic cross sections for the following reactions: 60 GeV=c protons with C, Be, Al targets and 120 GeV=c protons with C and Be targets. The analysis is performed using the NA61/SHINE spectrometer at the CERN Super Proton Synchrotron. First measurements are obtained using protons at 120 GeV=c, while the results for protons at 60 GeV=c are compared with previously published measurements. These interaction cross section measurements are critical inputs for neutrino flux prediction in current and future accelerator-based long-baseline neutrino experiments.Authors:A. Aduszkiewicz,15 E. V. Andronov,21 T. Antićić,3 V. Babkin,19 M. Baszczyk,13 S. Bhosale,10 A. Blondel,23 M. Bogomilov,2 A. Brandin,20 A. Bravar,23 W. Bryliński,17 J. Brzychczyk,12 M. Buryakov,19 O. Busygina,18 A. Bzdak,13 H. Cherif,6 M. Ćirković,22 M. Csanad,7 J. Cybowska,17 T. Czopowicz,17 A. Damyanova,23 N. Davis,10 M. Deliyergiyev,9 M. Deveaux,6 A. Dmitriev,19 W. Dominik,15 P. Dorosz,13 J. Dumarchez,4 R. Engel,5 G. A. Feofilov,21 L. Fields,24 Z. Fodor,7,16 A. Garibov,1 M. Gaździcki,6,9 O. Golosov,20 M. Golubeva,18 K. Grebieszkow,17 F. Guber,18 A. Haesler,23 S. N. Igolkin,21 S. Ilieva,2 A. Ivashkin,18 S. R. Johnson,26 K. Kadija,3 E. Kaptur,14 N. Kargin,20 E. Kashirin,20 M. Kiełbowicz,10 V. A. Kireyeu,19 V. Klochkov,6 V. I. Kolesnikov,19 D. Kolev,2 A. Korzenev,23 V. N. Kovalenko,21 K. Kowalik,11 S. Kowalski,14 M. Koziel,6 A. Krasnoperov,19 W. Kucewicz,13 M. Kuich,15 A. Kurepin,18 D. Larsen,12 A. László,7 T. V. Lazareva,21 M. Lewicki,16 K. Łojek,12 B. Łysakowski,14 V. V. Lyubushkin,19 M. Maćkowiak-Pawłowska,17 Z. Majka,12 B. Maksiak,11 A. I. Malakhov,19 A. Marchionni,24 A. Marcinek,10 A. D. Marino,26 K. Marton,7 H.-J. Mathes,5 T. Matulewicz,15 V. Matveev,19 G. L. Melkumov,19 A. O. Merzlaya,12 B. Messerly,27 Ł. Mik,13 G. B. Mills,25 S. Morozov,18,20 S. Mrówczyński,9 Y. Nagai ,26 M. Naskręt,16 V. Ozvenchuk,10 V. Paolone,27 M. Pavin,4,3 O. Petukhov,18 R. Płaneta,12 P. Podlaski,15 B. A. Popov,19,4 B. Porfy,7 M. Posiadała-Zezula,15 D. S. Prokhorova,21 D. Pszczel,11 S. Puławski,14 J. Puzović,22 M. Ravonel,23 R. Renfordt,6 E. Richter-Wąs,12 D. Röhrich,8 E. Rondio,11 M. Roth,5 B. T. Rumberger,26 M. Rumyantsev,19 A. Rustamov,1,6 M. Rybczynski,9 A. Rybicki,10 A. Sadovsky,18 K. Schmidt,14 I. Selyuzhenkov,20 A. Yu. Seryakov,21 P. Seyboth,9 M. Słodkowski,17 A. Snoch,6 P. Staszel,12 G. Stefanek,9 J. Stepaniak,11 M. Strikhanov,20 H. Ströbele,6 T. Šuša,3 A. Taranenko,20 A. Tefelska,17 D. Tefelski,17 V. Tereshchenko,19 A. Toia,6 R. Tsenov,2 L. Turko,16 R. Ulrich,5 M. Unger,5 F. F. Valiev,21 D. Veberič,5 V. V. Vechernin,21 A. Wickremasinghe,27 Z.Włodarczyk,9 A.Wojtaszek-Szwarc,9 K. Wójcik,14 O.Wyszyński,12 L. Zambelli,4 E. D. Zimmerman,26 and R. Zwaska24 (NA61/SHINE Collaboration) 1National Nuclear Research Center, Baku, Azerbaijan 2Faculty of Physics, University of Sofia, Sofia, Bulgaria 3Rud¯er Bošković Institute, Zagreb, Croatia 4LPNHE, University of Paris VI and VII, Paris, France 5Karlsruhe Institute of Technology, Karlsruhe, Germany 6University of Frankfurt, Frankfurt, Germany 7Wigner Research Centre for Physics of the Hungarian Academy of Sciences, Budapest, Hungary 8University of Bergen, Bergen, Norway 9Jan Kochanowski University in Kielce, Poland 10Institute of Nuclear Physics, Polish Academy of Sciences, Cracow, Poland 11National Centre for Nuclear Research, Warsaw, Poland 12Jagiellonian University, Cracow, Poland 13AGH—University of Science and Technology, Cracow, Poland 14University of Silesia, Katowice, Poland 15University of Warsaw, Warsaw, Poland 16University of Wrocław, Wrocław, Poland 17Warsaw University of Technology, Warsaw, Poland 18Institute for Nuclear Research, Moscow, Russia 19Joint Institute for Nuclear Research, Dubna, Russia 20National Research Nuclear University (Moscow Engineering Physics Institute), Moscow, Russia 21St. Petersburg State University, St. Petersburg, Russia 22University of Belgrade, Belgrade, Serbia 23University of Geneva, Geneva, Switzerland 24Fermilab, Batavia, Illinois, USA 25Los Alamos National Laboratory, Los Alamos, New Mexico, USA 26University of Colorado, Boulder, Colorado, USA 27University of Pittsburgh, Pittsburgh, Pennsylvania, US

Effect of scanning strategy on mechanical properties of Ti-6Al-4V alloy manufactured by laser direct energy deposition

In this work, we investigated the effect of DED thermal history on the obtained structural and mechanical properties of Ti-6Al-4V using a thermocouple. As a result of the study, the specific DED process parameters allow the same mechanical characteristics for as-built titanium alloy and the alloy after heat treatmen

Non-universal equilibrium crystal shape results from sticky steps

The anisotropic surface free energy, Andreev surface free energy, and equilibrium crystal shape (ECS) z=z(x,y) are calculated numerically using a transfer matrix approach with the density matrix renormalization group (DMRG) method. The adopted surface model is a restricted solid-on-solid (RSOS) model with "sticky" steps, i.e., steps with a point-contact type attraction between them (p-RSOS model). By analyzing the results, we obtain a first-order shape transition on the ECS profile around the (111) facet; and on the curved surface near the (001) facet edge, we obtain shape exponents having values different from those of the universal Gruber-Mullins-Pokrovsky-Talapov (GMPT) class. In order to elucidate the origin of the non-universal shape exponents, we calculate the slope dependence of the mean step height of "step droplets" (bound states of steps) using the Monte Carlo method, where p=(dz/dx, dz/dy)$, and represents the thermal averag |p| dependence of , we derive a |p|-expanded expression for the non-universal surface free energy f_{eff}(p), which contains quadratic terms with respect to |p|. The first-order shape transition and the non-universal shape exponents obtained by the DMRG calculations are reproduced thermodynamically from the non-universal surface free energy f_{eff}(p).Comment: 31 pages, 21 figure

ANALYSIS OF DOMAIN SPECIFICITY OF THE PROTECTIVE CHIMERIC ANTIBODY ch14D5a AGAINST GLYCOPROTEIN E OF TICK-BORNE ENCEPHALITIS VIRUS

A drug for the prevention and therapy of tick-borne encephalitis virus is being developed on the basis of the protective chimeric antibody ch14D5a. At the same time, the epitope recognized by this antibody on the surface of glycoprotein E has not been localized yet. The aim of this work was to identify the domain of glycoprotein E, to which the protective antibody ch14D5a binds. As a result, four recombinant variants of glycoprotein E were generated using the bacterial expression system: (1) the rE protein containing the domains D1, D2, and D3 of glycoprotein E; (2) the rED1+2 protein containing domains D1 and D2; (3) the rED3_301 protein, which is domain D3 of glycoprotein E, and (4) the rED3_294 protein comprising domain D3 and a hinge region connecting domains D1 and D3. The rED3_294 and rED3_301 proteins were obtained in soluble monomeric form. The rE and rED1+2 proteins were extracted from the inclusion bodies of Escherichia coli. Using Western blot analysis and surface plasmon resonance analysis, it was demonstrated that the protective chimeric antibody ch14D5a and its Fab fragment bound specifically to domain D3 of glycoprotein E. Since the antibodies recognizing epitopes on the surface of domain D3 do not tend to cause antibody-dependent enhancement of the infection as compared to antibodies directed to domains D1 and D2, the data obtained confirm the promise of using the antibody ch14D5a in the development of a therapeutic preparation against the tick-borne encephalitis virus

Изотопный состав кислорода и водорода повторно-жильных льдов Центрального Ямала

New data on the distribution of the isotopes δ18О and δD of ice wedges in Central Yamal are presented. In the summer of 2019, the massive wedge ice was studied in the thermal circus of the third marine terrace. On the surface of the terrace, polygonal-vein relief is common with a polygon size of about 10–20 m. The largest ice wedge № 1 in the upper part was 1.5 m wide, and its visible depth in the outcrop amounted to 2.3 m. Perpendicular to this wedge, another one № 2 was opened with a width of 60 cm, and 20 m from them the wedge № 3 was opened along the strike. At the level of the head of the wedge № 1, radiocarbon AMS dating was performed based on a sample of host deposits, which showed that the ice in the upper part was about 13 thousand years old (the ice age was of 13.6 cal. BP). According to the isotopic characteristics (average values of δ18О = −24.8, and δD = −187.6 ‰), the ice corresponds to the Yamal ice-wedge ice, which was formed during the MIS‑2 isotopic stage (the end of the Late Pleistocene). The isotopic composition of oxygen indicates temperatures of the cold period of the vein formation from −22.8 to −26.8 °C (on average 7 °C colder than the present-day) and the January temperatures from −34.2 to −40.2 °C (on average 10 °C colder than the present-day). For some ice samples (11 out of 43), high values of deuterium excesses (dexc = 12÷17 ‰) were observed, which is unusual for this type of ice. A few examples of high dexc values of ice wedges are mentioned in the literature for sections of Seyakha yedoma, Cape Sabler and Bolshoi Lyakhovsky Island, and note that for the ice dated to the Late Pleistocene. The deuterium excess values may reflect the unstable climatic conditions of the Late Pleistocene associated with changes in the vapor source and the trajectories of the air masses over the continent.Приведены новые данные о распределении δ18О и δD в повторно-жильном льду Центрального Ямала. По своим изотопным характеристикам (средние значения δ18О = −24,8, а δD = −187,6 ‰) лёд соответствует жильным льдам Ямала, которые формировались в изотопную стадию МИС‑2 (конец позднего плейстоцена). Для некоторых образцов льда отмечены высокие значения дейтериевого эксцесса (dexc = 12÷17 ‰), что нехарактерно для этого типа льдов. Немногочисленные свидетельства высоких значений dexc в позднеплейстоценовом жильном льду описаны в литературе для раз‑ резов р. Сеяха, мыса Саблера и о. Большой Ляховский