Searching for dominant high-level features for music information retrieval

Music Information Retrieval systems are often based on the analysis of a large number of low-level audio features. When dealing with problems of musical genre description and visualization, however, it would be desirable to work with a very limited number of highly informative and discriminant macro-descriptors. In this paper we focus on a specific class of training-based descriptors, which are obtained as the loglikelihood of a Gaussian Mixture Model trained with short musical excerpts that selectively exhibit a certain semantic homogeneity. As these descriptors are critically dependent on the training sets, we approach the problem of how to automatically generate suitable training sets and optimize the associated macro-features in terms of discriminant power and informative impact. We then show the application of a set of three identified macro-features to genre visualization, tracking and classification

Motion estimation and signaling techniques for 2D+t scalable video coding

We describe a fully scalable wavelet-based 2D+t (in-band) video coding architecture. We propose new coding tools specifically designed for this framework aimed at two goals: reduce the computational complexity at the encoder without sacrificing compression; improve the coding efficiency, especially at low bitrates. To this end, we focus our attention on motion estimation and motion vector encoding. We propose a fast motion estimation algorithm that works in the wavelet domain and exploits the geometrical properties of the wavelet subbands. We show that the computational complexity grows linearly with the size of the search window, yet approaching the performance of a full search strategy. We extend the proposed motion estimation algorithm to work with blocks of variable sizes, in order to better capture local motion characteristics, thus improving in terms of rate-distortion behavior. Given this motion field representation, we propose a motion vector coding algorithm that allows to adaptively scale the motion bit budget according to the target bitrate, improving the coding efficiency at low bitrates. Finally, we show how to optimally scale the motion field when the sequence is decoded at reduced spatial resolution. Experimental results illustrate the advantages of each individual coding tool presented in this paper. Based on these simulations, we define the best configuration of coding parameters and we compare the proposed codec with MC-EZBC, a widely used reference codec implementing the t+2D framework

Functional and Structural Biological Methods for Palytoxin Detection

Palytoxin (PLTX) and its analogues are marine polyethers identified in Palythoa and Zoanthus corals, Ostreopsis dinoflagellates, and Trichodesmium cyanobacteria. Humans can be exposed to these toxins by different routes with a series of adverse effects but the most severe risk is associated with poisonings by the consumption of edible marine organisms accumulating these toxins, as occurs in (sub)-tropical areas. In temperate areas, adverse effects ascribed to PLTXs have been recorded after inhalation of marine aerosols and/or cutaneous contact with seawater during Ostreopsis blooms, as well as during cleaning procedures of Palythoa-containing home aquaria. Besides instrumental analytical methods, in the last years a series of alternative or complementary methods based on biological/biochemical tools have been developed for the rapid and specific PLTX detection required for risk assessment. These methods are usually sensitive, cost- and time-effective, and do not require highly specialized operators. Among them, structural immunoassays and functional cellbased assays are reviewed. The availability of specific anti-PLTX antibodies allowed the development of different sensitive structural assays, suitable for its detection also in complex matrices, such as mussels. In addition, knowing the mechanism of PLTX action, a series of functional identification methods has been developed. Despite some of them being limited by matrix effects and specificity issues, biological methods for PLTX detection represent a feasible tool, suitable for rapid screening

Ultrastructural and spectrophotometric study on the effects of putative triggers on aortic valve interstitial cells in in vitro models simulating metastatic calcification.

Metastatic calcification of cardiac valves is a common complication in patients affected by chronic renal failure. In this study, primary bovine aortic valve interstitial cells (AVICs) were subjected to pro-calcific treatments consisting in cell stimulation with (i) elevated inorganic phosphate (Pi = 3mM), in order to simulate hyperphosphatemic conditions; (ii) bacterial endotoxin lipopolysaccharide (LPS), simulating direct effects by microbial agents; and (iii) conditioned media (CM) derived from cultures of either LPS-stimulated heterogenic macrophages (commercial murine RAW264.7 cells) or LPS-stimulated fresh allogeneic monocytes/macrophages (bCM), simulating consequent inflammatory responses, alone or combined. Compared to control cultures, spectrophotometric assays revealed shared treatment-dependent higher values of both calcium amounts and alkaline phosphatase activity for cultures involving the presence of elevated Pi. Ultrastructurally, shared peculiar pro-calcific degeneration patterns were exhibited by AVICs from the same cultures irrespectively of the applied treatment. Disappearance of all cytomembranes and concurrent formation of material showing positivity to Cuprolinic Blue and co-localizing with silver precipitation were followed by the outcropping of such a material, which transformed in layers outlining the dead cells. Subsequent budding of these layers resulted in the formation of bubbling bodies and concentrically laminated calcospherulae mirroring those in actual soft tissue calcification. In conclusion, the in vitro models employed appear to be reliable tools for simulating metastatic calcification and indicate that hyperphosphatemic-like conditions could trigger valve calcification per se, with LPS and allogeneic macrophage-derived secretory products acting as possible calcific enhancers via inflammatory responses

Experimental evaluation of a localization algorithm for multiple acoustic sources in reverberating environments

Publication in the conference proceedings of EUSIPCO, Florence, Italy, 200

Survival-related autophagic activity versus procalcific death in cultured aortic valve interstitial cells treated with critical normophosphatemic-like phosphate concentrations

Valve dystrophic calcification is a common disorder affecting normophosphatemic subjects. Here, cultured aortic valve interstitial cells (AVICs) were treated 3 to 28 days with phosphate (Pi) concentrations spanning the normal range in humans (0.8, 1.3, and 2.0 mM) alone or supplemented with proinflammatory stimuli to assess possible priming of dystrophic-like calcification. Compared with controls, spectrophotometric analyses revealed marked increases in calcium amounts and alkaline phosphatase activity for 2.0-mM-Pi-containing cultures, with enhancing by proinflammatory mediators. Ultrastructurally, AVICs treated with low/middle Pi concentrations showed an enormous endoplasmic reticulum (ER) enclosing organelle debris, so apparently executing a survival-related atypical macroautophagocytosis, consistently with ultracytochemical demonstration of ER-associated acid phosphatase activity and decreases in autophagosomes and immunodetectable MAP1LC3. In contrast, AVICs cultured at 2.0-mM Pi underwent mineralization due to intracellular release and peripheral layering of phospholipid-rich material acting as hydroxyapatite nucleator, as revealed by Cuprolinic Blue and von Kossa ultracytochemical reactions. Lack of immunoblotted caspase-3 cleaved form indicated apoptosis absence for all cultures. In conclusion, fates of cultured AVICs were crucially driven by Pi concentration, suggesting that serum Pi levels just below the upper limit of normophosphatemia in humans may represent a critical watershed between macroautophagy-associated cell restoring and procalcific cell death