Avian response to early tidal salt marsh restoration at former commercial salt evaporation ponds in San Francisco Bay, California, USA

Restoration of former commercial salt evaporation ponds in the San Francisco Bay estuary is intended to reverse a severe decline (\u3e79%) in tidal salt marshes. San Francisco Bay is a critical migratory stopover site and wintering area for shorebirds and waterfowl, and salt ponds are important high tide roosting and foraging areas. Conservation of past bird abundance is a stated goal of area restoration projects, and early adaptive management will be critical for achieving this objective. However, initial avian response at sites restored to tidal flow may not be indicative of long-term results. For example, winter shorebirds at a 529 ha pond breached in 2002 showed a marked increase in shorebird abundance following breaching. Shorebirds comprised 1% of area totals during 1999-2002 and increased to 46% during 2003-2008. These changes accompanied increased tidal range and sedimentation, but minimal vegetation establishment. Conversely, a fully vegetated, restored 216 ha pond in the same system consistently supported less than 2% of all waterbirds in the region. Early restoration may temporarily increase habitat, but managed ponds will be needed for long-term waterbird abundance within a restored pond-marsh system

Large Bi-2212 single crystal growth by the floating-zone technique

Effects of the growth velocity on the crystal growth behavior of Bi_2Sr_2Ca_1Cu_2O_x (Bi-2212) have been studied by floating zone technique. The results show that a necessary condition for obtaining large single crystals along the c-axis is that the solid-liquid interface of a growing rod maintains a stable planar growth front. The planar liquid-solid growth interface tends to break down into a cellular interface, while the growth velocity is higher than 0.25 mm/h. Single crystals of up to 50x7.2x7 mm3 along the a-, b- and caxes have been cut in a 7.2 mm diameter rod with optimum growth conditions. Tconset is 91 K measured by magnetic properties measurement system (MPMS) for as-grown crystals. Optical polarization microscope and neutron diffraction show that the quality of the single crystals is good.Comment: 5 pages, 4 figure

Waterbird monitoring and habitat association modeling to inform tidal marsh restoration in an urbanized estuary

The San Francisco Bay (SFB), like many other urbanized estuaries, is a critical wintering and stop-over area for migratory waterbirds. More than a million wintering waterbirds annually rely on a mosaic of natural and managed habitats in SFB, including former salt ponds. The South Bay Salt Pond Restoration Project plans restore 50 to 90% of a 6,100 hectare former salt production pond complex to tidal marsh, while maintaining the rest as foraging and roosting areas for migratory birds. Since 2002, we have evaluated migratory waterbird use of pre- and post-restoration salt ponds in south SFB. Our approach has been to use monthly surveys, applied studies and modeling to evaluate avian response to this changing habitat. Trend analyses indicate that overall waterbird numbers have nearly doubled across the complex from 2003-2014. Dabbling ducks increased significantly during initial phases of the project, while diving ducks, small and medium shorebird abundances declined initially, but rebounded in recent years. We modeled relationships between abundances of multiple species and habitat characteristics at both landscape and micro-scales. Modeling results suggest that the importance of different habitat characteristics varies among waterbird guilds. For example, foraging diving duck abundances were higher in deep, un-breached ponds, located close to the edge of SFB, while foraging small shorebirds predominated in shallow, breached ponds containing islands. Our research highlights important habitat characteristics both within and across wetland areas, and provides a unique opportunity to guide future management and restoration decisions for multiple species across large landscapes in SFB and other urbanized estuaries

A Remote Sensing Approach to Assess the Historical Invasion of \u3cem\u3ePhragmites australis\u3c/em\u3e in a Brackish Coastal Marsh

Introduction: Coastal estuarine wetlands provide important habitats for a variety of endemic flora and fauna but are particularly vulnerable to biological invasions. Regular monitoring of changes in these vulnerable wetlands has become increasingly important for effective management, especially considering threats from climate change effects and human disturbance. Historical analyzes of plant invasions may guide targeted management strategies to eradicate harmful species. Estimating the distribution of invasive species has never been more accessible with the improved availability of high-resolution data and innovations in remote sensing, estimating the distribution of invasive species has never been more accessible. Methods: We assessed the spread of non-native Phragmites australis subsp. australis in Suisun Marsh on the upper San Francisco Estuary, one of the largest brackish coastal wetlands in North America. Suisun Marsh consists of managed and tidal wetlands, and efforts have been made to control invasive P. australis on the managed wetlands to support habitat values for wildlife. We used remote-sensing analyzes of publicly available, biennial color-infrared images taken by the National Agriculture Imagery Program (NAIP) to map the expansion of invasive P. australis across two decades. We generated random forest classifications of representative images to map the distribution of P. australis, then calculated a variety of metrics describing the rate and spatial extent of the P. australis spread. Additionally, we ran generalized linear models to examine factors related to the growth of P. australis. Results: Our classifications yielded accuracies of over 90% and showed a 234% (1,084 ha) increase in P. australis between 2003 and 2018. The expansion rate of P. australis patches averaged 1.32 m/year (±0.53 SD) which is higher than most reported in the literature. We found that P. australis expansion in managed areas within levees was significantly correlated with invasion in tidal areas outside the levees on the same parcel and also related to its spread on adjacent parcels. Discussion: Our findings suggest that despite individual landowner management efforts, P. australis has continued to expand substantially throughout Suisun Marsh. Future efforts to treat invasive P. australis may require emphasizing adaptive, collaborative management rather than individual management strategies to ensure the invasive species is eradicated on a large scale to preserve the valued ecosystem functions

Nobeyama Cygnus-X Survey: Physical Properties of C18^{18}O clumps in DR-6(W), DR-9 and DR-13S regions

Cygnus-X is considered a region of interest for high-energy astrophysics, since the Cygnus OB2 association has been confirmed as a PeVatron in the Cygnus cocoon. In this research note, we present new high-resolution (16'') $^{12,13}$CO(J=1$\rightarrow$0) and C$^{18}$O (J=1$\rightarrow$0) observations obtained with the Nobeyama 45-m radiotelescope, to complement the Nobeyama Cygnus-X Survey. We discovered 19 new C$^{18}$O clumps associated with the star-forming regions DR-6W, DR-9, and DR13S. We present the physical parameters of these clumps, which are consistent with the neighboring covered regions. We confirm the clumpy nature of these regions and of a filament located between DR6 and DR6W. These results strongly suggest that star formation occurs in these regions with clumps of sizes $\sim$10$^{-1}$ pc, masses $\sim$10$^2$ M$_\odot$, and H$_2$ densities of $\sim$10$^4$ cm$^{-3}$.Comment: 8 pages, 3 Figures, 1 Table. https://pos.sissa.it/444/631/pd

Endothelin receptor B antagonists decrease glioma cell viability independently of their cognate receptor

Background: Endothelin receptor antagonists inhibit the progression of many cancers, but research into their influence on glioma has been limited. Methods: We treated glioma cell lines, LN-229 and SW1088, and melanoma cell lines, A375 and WM35, with two endothelin receptor type B (ETRB)-specific antagonists, A-192621 and BQ788, and quantified viable cells by the capacity of their intracellular esterases to convert non-fluorescent calcein AM into green-fluorescent calcein. We assessed cell proliferation by labeling cells with carboxyfluorescein diacetate succinimidyl ester and quantifying the fluorescence by FACS analysis. We also examined the cell cycle status using BrdU/propidium iodide double staining and FACS analysis. We evaluated changes in gene expression by microarray analysis following treatment with A-192621 in glioma cells. We examined the role of ETRB by reducing its expression level using small interfering RNA (siRNA). Results: We report that two ETRB-specific antagonists, A-192621 and BQ788, reduce the number of viable cells in two glioma cell lines in a dose- and time-dependent manner. We describe similar results for two melanoma cell lines. The more potent of the two antagonists, A-192621, decreases the mean number of cell divisions at least in part by inducing a G2/M arrest and apoptosis. Microarray analysis of the effects of A-192621 treatment reveals up-regulation of several DNA damage-inducible genes. These results were confirmed by real-time RT-PCR. Importantly, reducing expression of ETRB with siRNAs does not abrogate the effects of either A-192621 or BQ788 in glioma or melanoma cells. Furthermore, BQ123, an endothelin receptor type A (ETRA)-specific antagonist, has no effect on cell viability in any of these cell lines, indicating that the ETRB-independent effects on cell viability exhibited by A-192621 and BQ788 are not a result of ETRA inhibition. Conclusion: While ETRB antagonists reduce the viability of glioma cells in vitro, it appears unlikely that this effect is mediated by ETRB inhibition or cross-reaction with ETRA. Instead, we present evidence that A-192621 affects glioma and melanoma viability by activating stress/DNA damage response pathways, which leads to cell cycle arrest and apoptosis. This is the first evidence linking ETRB antagonist treatment to enhanced expression of DNA damage-inducible genes

Do Bar-Headed Geese Train for High Altitude Flights?

This is the author accepted manuscript. The final version is available from OUP via the DOI in this recordSYNOPSIS: Exercise at high altitude is extremely challenging, largely due to hypobaric hypoxia (low oxygen levels brought about by low air pressure). In humans, the maximal rate of oxygen consumption decreases with increasing altitude, supporting progressively poorer performance. Bar-headed geese (Anser indicus) are renowned high altitude migrants and, although they appear to minimize altitude during migration where possible, they must fly over the Tibetan Plateau (mean altitude 4800 m) for much of their annual migration. This requires considerable cardiovascular effort, but no study has assessed the extent to which bar-headed geese may train prior to migration for long distances, or for high altitudes. Using implanted loggers that recorded heart rate, acceleration, pressure, and temperature, we found no evidence of training for migration in bar-headed geese. Geese showed no significant change in summed activity per day or maximal activity per day. There was also no significant change in maximum heart rate per day or minimum resting heart rate, which may be evidence of an increase in cardiac stroke volume if all other variables were to remain the same. We discuss the strategies used by bar-headed geese in the context of training undertaken by human mountaineers when preparing for high altitude, noting the differences between their respective cardiovascular physiology.This work was supported by the UK Biotechnology and Biological Sciences Research Council [BBSRC; BB/FO15615/1 to C.M.B. and P.J.B.]. Authors were supported by a Natural Sciences and Engineering Research Council of Canada (NSERC) award [W.K.M.], and the FAO through the Animal Health Service EMPRES surveillance program