Penapisan Aktivitas Superoksida Dismutase Pada Berbagai Tanaman

Free radicals can be damaged on cell integration, because free radicals very reactive to cell component either structur component or functional component. To scavenge and inhibit chain reaction of free radicals were required exogen antioxidant. Natural antioxidant from plant was more interested because its more safe than synthetic antioxidant. Plant in the world such as spices, vegetables, fruits, medicine plants much contain antioxidant. Antioxidant activity to dismutate superoxide radical (O2-) from various plant was expected capable to help know potential natural antioxidant resources. This research was found out that salam leaf (Eugenia polyanta Wight), sappan wood (Caesalpinia sappan L.), seed and fruit guava have (Psidium guajava L.) superoxide dismutase (SOD) activity 100%

CHEMICAL CONSTITUENTS FROM THE STEM BARK OF Alangium kurzi, Craib

Three new chemical constituents have been isolated from the stem bark of Alangium kurzii Craib. The structures were respectively determined to be 4-cyclohexene-1,2,3-triol 1, 3-β-stigmast-5-en-3-ol (β-sitosterol 2), and 3-O-β-D-glucopyranosyl-stigmast-5-ene (daucosterol 3). These compounds are preliminary isolated from this plant. Keyword: Alangium kurzii, Alangiaceae, 4-cyclohexene-1,2,3-triol, β-sitosterol, daucosterol

Identification and Functional Testing of Novel Interacting Protein Partners for the Stress Sensors Wsc1p and Mid2p of \u3cem\u3eSaccharomyces cerevisiae\u3c/em\u3e

Wsc1p and Mid2p are transmembrane signaling proteins of cell wall stress in the budding yeast Saccharomyces cerevisiae. When an environmental stress compromises cell wall integrity, they activate a cell response through the Cell Wall Integrity (CWI) pathway. Studies have shown that the cytoplasmic domain of Wsc1p initiates the CWI signaling cascade by interacting with Rom2p, a Rho1-GDP-GTP exchange factor. Binding of Rom2p to the cytoplasmic tail of Wsc1p requires dephosphorylation of specific serine residues but the mechanism by which the sensor is dephosphorylated and how it subsequently interacts with Rom2p remains unclear. We hypothesize that Wsc1p and Mid2p must be physically associated with interacting proteins other than Rom2p that facilitate its interaction and regulate the activation of CWI pathway. To address this, a cDNA plasmid library of yeast proteins was expressed in bait strains bearing membrane yeast two-hybrid (MYTH) reporter modules of Wsc1p and Mid2p, and their interacting preys were recovered and sequenced. 14 previously unreported interactors were confirmed for Wsc1p and 29 for Mid2p. The interactors’ functionality were assessed by cell growth assays and CWI pathway activation by western blot analysis of Slt2p/Mpk1p phosphorylation in null mutants of each interactor under defined stress conditions. The susceptibility of these strains to different stresses were tested against antifungal agents and chemicals. This study reports important novel protein interactions of Wsc1p and Mid2p that are associated with the cellular response to oxidative stress induced by Hydrogen Peroxide and cell wall stress induced by Caspofungin

Toward the Discovery of Biological Functions Associated with the Mechanosensor Mtl1p of \u3ci\u3eSaccharomyces cerevisiae\u3c/i\u3e via Integrative Multi-OMICs Analysis

Functional analysis of the Mtl1 protein in Saccharomyces cerevisiae has revealed that this transmembrane sensor endows yeast cells with resistance to oxidative stress through a signaling mechanism called the cell wall integrity pathway (CWI). We observed upregulation of multiple heat shock proteins (HSPs), proteins associated with the formation of stress granules, and the phosphatase subunit of trehalose 6-phosphate synthase which suggests that mtl1Δ strains undergo intrinsic activation of a non-lethal heat stress response. Furthermore, quantitative global proteomic analysis conducted on TMT-labeled proteins combined with metabolome analysis revealed that mtl1Δ strains exhibit decreased levels of metabolites of carboxylic acid metabolism, decreased expression of anabolic enzymes and increased expression of catabolic enzymes involved in the metabolism of amino acids, with enhanced expression of mitochondrial respirasome proteins. These observations support the idea that Mtl1 protein controls the suppression of a non-lethal heat stress response under normal conditions while it plays an important role in metabolic regulatory mechanisms linked to TORC1 signaling that are required to maintain cellular homeostasis and optimal mitochondrial function

BIODEGRADATION TEST ON THE LUBRICATING OIL IN THE FRESH AND SEA WATER OF THE BACTERIA FROM SOIL CONTAMINATED LUBRICATING OIL

Telah dilakukan penelitian degradasi minyak pelumas bekas oleh bakteri asal tanah yang terkontaminasi minyak pelumas bekas dalam media air tawar dan air laut. Penelitian ini bertujuan untuk memperoleh isolat bakteri yang mempunyai kemampuan mendegradasi tinggi terhadap minyak pelumas bekas dalam media air tawar dan air laut. Bakteri hasil isolasi diidentifikasi dan diseleksi terhadap minyak pelumas bekas. Hasil seleksi diaklimatisasi untuk mendapatkan bakteri yang akan diuji pada media pelarut air laut. Hasil seleksi dan aklimatisasi memperlihatkan Arthobacter sp., Bacillus sp., dan Pseudomonas sp. mempunyai degradasi yang tinggi. Uji biodegradasi dilakukan dalam media Bushnell-Haas Gram Mineral dengan pelarut air tawar dan air laut dengan bakteri uji berupa kultur tunggal dan campuran. Hasil identifikasi ditemukan 10 isolat bakteri. Degradasi terbesar pada air laut adalah kultur tunggal Bacillus sp. (52.74%), sedangkan pada air tawar adalah pada kultur campuran Arthrobacter sp., Bacillus sp., dan Pseudomonas sp (37.3%)