48 research outputs found
Evaluation of the immunity factors (THC, TPP, PO, SOD, POD) of shrimp fed with the yeast Saccharomyces cerevisiae compared to shrimp fed without yeast
Effects of S. cerevisiae on immune parameters and the WSSV resistance of the L. vannamei after 10 days of S. cerevisiae feeding were evaluated in this study. The experimental diet has the commercial shrimp composition, but 2 g of S. cerevisiae substituted 2 g of fish meal. Six hundred of subadult shrimp (10±1.02 g) were collected and divided to four groups; the 1st group (T1) and the 2nd group (T2) fed experimental diet for 10 days and T2 after 10 days injected with WSSV, whereas the 3ed group (T3) and 4th group (T4) fed with commercial diet for 10 days as control negative and control positive and T4 after 10 days injected with WSSV and all group maintained for 25 days. The immune parameters such as total hemocyte count (THC), total plasma protein (TPP), phenoloxidase activity (PO), superoxide dismutase (SOD) activity, peroxidase (POD) activity were measured. Shrimp immune parameter in T1 and T2 was significantly increased comparing to T3 and T4, but in T2 the immune response decreased in contrast to T1. The immune parameter in T4 also significantly decreased in contrast to T3. Survival rate in T1 and T3 was higher and significantly different (P<0.05) with T2 and T4, whereas, T2 showed significantly different (P<0.05) survival rate with T4 at the end of experiment. In conclusion these results suggest that the increased survival rate and resistance of shrimp after S. cerevisiae consumption occurs through immune modifications, such as increases in THC, TPP, SOD, SOP and PO activity, against WSSV
Understanding Change in Romantic Relationship Expectations of International Female Students from Turkey
In the light of grounded theory, the authors explored change in romantic relationship expectations of international students. Twelve female graduate students from Turkey were interviewed and several themes were identified explaining the presence and absence of change in participants’ attitudes toward romantic relationships. The findings are discussed in relation to acculturation and direction for future research is presented
Standardization of insurance operations and risk assessment and edition of manual for recognition of management and natural disaster risks in shrimp farms, and feasibility study of insuring shrimp hatcheries
According to achieve sustainable production in shrimp farming industry, it is necessary to provide the relief of producers through insurance. Aquaculture faces various environmental conditions such as unpredictable climate changes, epidemic diseases, harsh water factors, that may affect the production cycle, and impact on producer's life. In this research, the effects of various factors on production of cultured shrimp have been studied. The project conducted through completion of questionaries by random selected of small scale farms, and all large farms which engaged in production in that years. In order to increase the accuracy of responses, special questionnaires provided to fill in by expertise experts. All data explained the share of management and natural factors on production process
Identification and characterization of pathogenic viruses in aquatic animal using advanced techniques in order to develop rapid diagnostic kit (shrimp viral disease kit as well as TSV, HPV and MBV )
Shrimp production increasing rapidly in the world and in2013 the production reaches 4.2 MT. In Iran the shrimp production is under development and estimated in 1393, 20 thousand tons produced. In this regards the important subject is health and disease in shrimp farm. The white spot syndrome virus for second time appears in chabahar and damage many farms. Because the aquaculture activity expand in the world in national, regional and international scale, many emerge disease are endanger. In this regard the viral disease is very important and not only decrease the production but also has a side effect in business and national economy. For control and prevention the viral disease, the accurate methods such as PCR kit were developed. In this project the PCR methods with sensitivity, specificity and efficacy was designed and used for detection viral disease. Many viruses have several serotypes and in different area maybe new serotype induce the disease. For this reason, the specific kit will be design. Three viruses consist of MBV, TSV and IHHNV are very pathogenic in shrimp farm and need the specific PCR kit for detection them. In this project the MBV virus was identified and designs a new primer with Oligo software and the primer amplified a part of DNA with 185 bp in the gel. The specificity and sensitivity of primer were checked by IQ2000 Kit and the primer used for detection unknown samples
TAMANHO DE PARCELA E NÚMERO DE REPETIÇÕES PARA MAMONEIRA EM DIFERENTES ESPAÇAMENTOS ENTRE PLANTAS
In vitro Osteogenic impulse effect of Dexamethasone on periodontal ligament stem cells
Periodontium is a complex organ composed of mineralized epithelial and connective tissue. Dexamethasone could stimulate proliferation of osteoblast and fibroblasts. This study aimed to assess the osteogenic effect of dexamethasone on periodental ligament (PDL) stem cells. PDL stem cells were collected from periodontal ligament tissue of root of extracted premolar of young and healthy people. The stem cells were cultured in alpha-MEM Medium in three groups, one group with basic medium contains (alpha-MEM and FBS 10 and 50 mmol of beta gelisrophosphat and L ascorbic acid mu g/ml), the second group: basic medium with dexamethasone and the third one: basic medium without any osteogenic stimulant. Mineralization of cellular layer was analyzed with Alizarin red stain method. Osteogenic analysis was done by Alkaline phosphates and calcium test. These analysis indicated that the amount of intra-cellular calcium and alkaline phosphates in the Dexamethasone group was far more than the control and basic group (P<0.05). The results of Alizarin red stain indicated more mineralization of cultured cells in Dexamethasone group (P<0.05). The study results showed that Dexamethasone has significant osteogenic effect on PDL stem cells and further studies are recommended to evaluate its effect on treatment of bone disorders
Protective Effects of Lycopene and Ellagic Acid on Gonadal Tissue, Maternal Newborn Rats Induced by Cadmiumchloride
Background & aim: Cadmium is a toxin which reduces the ability of the reproduction in humans .Different antioxidants damaging effects of toxins are eliminated .The purpose of this study was to investigate the protective effects of lycopene and Ellagic acid induced by cadmium chloride on the gonadal tissue of newborn rats during pregnancy.
Methods: In the present experimental study, 30 adult female Wistar rats (180-200 gr) were prepared and maintained in standard conditions. The female rats were used for mating with the male. After observation of vaginal plaque, pregnant rats were randomly divided into 5 groups of 6 rats. Group I (normal): They were given normal saline in 13 days during pregnancy. Group II (Control): Cadmium chloride (1.5 mg / kg/ IP) was injected and normal saline was given to them in 13 days of during pregnancy. Group III: Cadmium chloride (1.5 mg / kg/ IP) was injected and ellagic acid (10 mg/kg/orally) in 13 days were injected during pregnancy. Group IV: Cadmium chloride (1.5 mg / kg/ IP) was injected and copene acid (20 mg/kg/orally) was injected in 13 days of during pregnancy. Group V: Cadmium chloride (1.5 mg / kg/ IP) was injected and ellagic acid (10 mg/kg/orally) and lycopene acid (20 mg/kg/orally) were injected in 13 days during pregnancy. After postpartum, Neonatal rats were anesthetized with ether. Animals were dissected, then the testes and Ovaries were removed and transferred to 10% formalin solution. After tissue processing, tissue sections were prepared and H&E stained. Data were analyzed by SPSS software and ANOVA test.
Results: Average number of Sertoli cells ,spermatogonia ,Leydig, and the number of seminiferous tube in control group were compared to other groups that were treated with lycopene - ellagic acid and ellagic acid had been reduced-proves to be significant(P <0.05). Average diameter of seminiferous tube in control group compared to other groups that are treated with lycopene - ellagic acid and ellagic acid had been reduced-proves but is not significant. Mean primordial follicle diameter and number of primordial of ovary tissue in control group compared to other groups that were treated with lycopene - ellagic acid and ellagic acid had been increased-proved to be significant(P <0.05).
Conclusion: The results showed that the combined treatment of ellagic acid and lycopene-ellagic acid may have reduced the toxic effects of cadmium chloride and may have improved the process of replicating the sex cells