Investigation of the shell stars omicron and theta Per, and of the eclipsing binary beta Lyr

All three stars showed rather complicated spectra, which require a very detailed spectroscopic analysis. The far UV spectrum of Beta Lyrae is clearly peculiar, with a multitude of emission lines not observed on any other star so far scanned with Copernicus. This made this star at once the most interesting and also, in a sense, easier to study. The other two stars display a spectrum rich in absorption lines, some of them being fairly broad (as expected for photospheric lines of rapidly rotating objects), some sharp. The later were clearly non-photospheric lines. An attempt was made to distinguish the circumstellar from the interstellar components

Evolution of close binary systems: Observational aspects

Detached close binary systems define the main sequence band satisfactorily, but very little is known about the masses of giants and supergiants. High dispersion international ultraviolet explorer satellite observations promise an improvement, since blue companions are now frequently found to late type supergiants. Mu Sagittaril and in particular Xi Aurigae are discussed in more detail. The barium star abundance anomaly appears to be due to mass transfer in interacting systems. The symbiotic stars are another type of binary systems containing late type giants; several possible models for the hotter star and for the type of interaction are discussed. The W Serpentis stars appear to be Algols in the rapid phase of mass transfer, but a possible link relating them to the symbiotics is also indicated. Evidence of hot circumstellar plasmas has now been found in several ordinary Algols; there may exist a smooth transition between very quiescent Algols and the W Serpentis stars. Beta Lyrae is discussed in the light of new spectrophotometric results

The Roche problem: some analytics

Some exact analytical formulas are derived for the potential and mass ratio as a function of Lagrangian points position, in the classical Roche model of the close binary stars.Comment: aastex, 4 pp. ApJ accepte

Analysis of the symbiotic star AG Pegasi

High and low dispersion IUE data are analyzed in conjunction with coincident ground based spectrophotometric scans and supplementary infrared photometry of the symbiotic object AG Pegasi. The IUE observations yield an improved value of E(B-V) = 0.12. The two stellar components are easily recognized in the spectra. The cool component may be an M1.7 III star and the hot component appears to have T (sub eff) of approximately 30000 K. The emission lines observed in the ultraviolet indicate two or three distince emitting regions. Nebular component ultraviolet intercombination lines suggest an electron density of several times 10 billion/cu cm

Synthetic NLTE accretion disc spectra for the dwarf nova SS Cyg during an outburst cycle

Dwarf nova outbursts result from enhanced mass transport through the accretion disc of a cataclysmic variable system. We assess the question of whether these outbursts are caused by an enhanced mass transfer from the late-type main sequence star onto the white dwarf (so-called mass transfer instability model, MTI) or by a thermal instability in the accretion disc (disc instability model, DIM). We compute non-LTE models and spectra of accretion discs in quiescence and outburst and construct spectral time sequences for discs over a complete outburst cycle. We then compare our spectra to published optical spectroscopy of the dwarf nova SS Cygni. In particular, we investigate the hydrogen and helium line profiles that are turning from emission into absorption during the rise to outburst. The evolution of the hydrogen and helium line profiles during the rise to outburst and decline clearly favour the disc-instability model. Our spectral model sequences allow us to distinguish inside-out and outside-in moving heating waves in the disc of SS Cygni, which can be related to symmetric and asymmetric outburst light curves, respectively.Comment: 8 pages, 8 figures; accepted to A&

The "K-Correction" for Irradiated Emission Lines in LMXBs: Evidence for a Massive Neutron Star in X1822-371 (V691 CrA)

We study the K-correction for the case of emission lines formed in the X-ray illuminated atmosphere of a Roche lobe filling star. We compute the K-correction as function of the mass ratio 'q' and the disc flaring angle 'alpha' using a compact binary code where the companion's Roche lobe is divided into 10^5 resolution elements. We also study the effect of the inclination angle in the results. We apply our model to the case of the neutron star low-mass X-ray binary X1822-371 (V691 CrA), where a K-emission velocity K_em=300 +-8 km/s has been measured by Casares et al. (2003). Our numerical results, combined with previous determination of system parameters, yields 1.61Msun < M_NS < 2.32Msun and 0.44Msun < M_2 < 0.56Msun for the two binary components(i. e. 0.24 < q < 0.27), which provide a compelling evidence for a massive neutron star in this system. We also discuss the implications of these masses into the evolutionary history of the binary.Comment: 6 pages, 5 figures. Accepted for publication in Ap

Equipotential Surfaces and Lagrangian points in Non-synchronous, Eccentric Binary and Planetary Systems

We investigate the existence and properties of equipotential surfaces and Lagrangian points in non-synchronous, eccentric binary star and planetary systems under the assumption of quasi-static equilibrium. We adopt a binary potential that accounts for non-synchronous rotation and eccentric orbits, and calculate the positions of the Lagrangian points as functions of the mass ratio, the degree of asynchronism, the orbital eccentricity, and the position of the stars or planets in their relative orbit. We find that the geometry of the equipotential surfaces may facilitate non-conservative mass transfer in non-synchronous, eccentric binary star and planetary systems, especially if the component stars or planets are rotating super-synchronously at the periastron of their relative orbit. We also calculate the volume-equivalent radius of the Roche lobe as a function of the four parameters mentioned above. Contrary to common practice, we find that replacing the radius of a circular orbit in the fitting formula of Eggleton (1983) with the instantaneous distance between the components of eccentric binary or planetary systems does not always lead to a good approximation to the volume-equivalent radius of the Roche-lobe. We therefore provide generalized analytic fitting formulae for the volume-equivalent Roche lobe radius appropriate for non-synchronous, eccentric binary star and planetary systems. These formulae are accurate to better than 1% throughout the relevant 2-dimensional parameter space that covers a dynamic range of 16 and 6 orders of magnitude in the two dimensions.Comment: 12 pages, 10 figures, 2 Tables, Accepted by the Astrophysical Journa

Identification and characterisation of G-quadruplex DNA-forming sequences in the Pseudomonas aeruginosa genome

A number of Gram-negative bacteria such as Pseudomonas aeruginosa are becoming resistant to front-line antibiotics. Consequently, there is a pressing need to find alternative bio-molecular targets for the development of new drugs. Since non-canonical DNA structures such as guanine-quadruplexes (G4s) have been implicated in regulating transcription, we were interested in determining whether there are putative quadruplex-forming sequences (PQS) in the genome of Pseudomonas aeruginosa. Using bioinformatic tools, we screened 36 genes potentially relevant to drug resistance for the presence of PQS and 10 of these were selected for biophysical characterisation (i.e. circular dichroism and thermal difference UV/Vis spectroscopy). These studies showed that three of these G-rich sequences (linked to murE, ftsB and mexC genes) form stable guanine-quadruplexes which were studied by NMR spectroscopy; detailed analysis of one of the sequences (mexC) confirmed that it adopts a two-quartet antiparallel quadruplex structure in the presence of K+ ions. We also show by FRET melting assays that small molecules can stabilise these three new G4 DNA structures under physiological conditions. These initial results could be of future interest in the development of new antibiotics with alternative bio-molecular targets which in turn would help tackle antimicrobial resistance

Modifiable Risk Factors for Common Ragweed (Ambrosia artemisiifolia) Allergy and Disease in Children: A Case-Control Study

Ragweed allergy is a major public health concern. Within Europe, ragweed is an introduced species and research has indicated that the amounts of ragweed pollen are likely to increase over Europe due to climate change, with corresponding increases in ragweed allergy. To address this threat, improving our understanding of predisposing factors for allergic sensitisation to ragweed and disease is necessary, specifically focusing upon factors that are potentially modifiable (i.e., environmental). In this study, a total of 4013 children aged 2–13 years were recruited across Croatia to undergo skin prick tests to determine sensitisation to ragweed and other aeroallergens. A parental questionnaire collected home environment, lifestyle, family and personal medical history, and socioeconomic information. Environmental variables were obtained using Geographical Information Systems and data from nearby pollen, weather, and air pollution stations. Logistic regression was performed (clustered on school) focusing on risk factors for allergic sensitisation and disease. Ragweed sensitisation was strongly associated with ragweed pollen at levels over 5000 grains m–3 year−1 and, above these levels, the risk of sensitisation was 12–16 times greater than in low pollen areas with about 400 grains m–3 year−1. Genetic factors were strongly associated with sensitisation but nearly all potentially modifiable factors were insignificant. This included measures of local land use and proximity to potential sources of ragweed pollen. Rural residence was protective (odds ratio (OR) 0.73, 95% confidence interval (CI) 0.55–0.98), but the factors underlying this association were unclear. Being sensitised to ragweed doubled (OR 2.17, 95% CI 1.59–2.96) the risk of rhinoconjunctivitis. No other potentially modifiable risk factors were associated with rhinoconjunctivitis. Ragweed sensitisation was strongly associated with ragweed pollen, and sensitisation was significantly associated with rhinoconjunctivitis. Apart from ragweed pollen levels, few other potentially modifiable factors were significantly associated with ragweed sensitisation. Hence, strategies to lower the risk of sensitisation should focus upon ragweed control

Fullerenol nanoparticles as a new delivery system for doxorubicin

Doxorubicin is a very potent chemotherapeutic drug, however its side effects limit its clinical use. The aim of this research was to investigate the properties of a fullerenol/doxorubicin nanocomposite, its potentially cytotoxic and genotoxic effects on malignant cell lines, as well as its toxicity towards zebra fish embryos. Chromatographic, NMR and mass spectral analysis of the nanocomposite imply that interactions between doxorubicin and fullerenol are non-covalent bonds. The stability of the nanocomposite was confirmed by the use of atomic force microscopy, dynamic light scattering and transmission electron microscopy. The nanocomposite, compared to the free doxorubicin at equivalent concentrations, significantly decreased the viability of MCF-7 and MDA-MB-231 cells. The flow cytometry results indicated that doxorubicin-loaded fullerenol could remarkably increase the uptake of doxorubicin suggesting that fullerenol might be a promising intracellular targeting carrier for the efficient delivery of antitumor drugs into tumor cells. The nanocomposite also affected cell cycle distribution. A genotoxicity test showed that the nanocomposite at all examined concentrations on MCF-7 and at lower concentrations on MDA-MB-231 cells caused DNA damage. Consequently, cell proliferation was notably reduced when compared with controls. Results of the zebrafish embryotoxicity assay showed a decreased overall toxicity, particularly cardiotoxicity and increased safety of the nanocomposite in comparison to doxorubicin alone, as manifested by a higher survival of embryos and less pericardial edema