49 research outputs found
Bisphenol A and 17β-Estradiol Promote Arrhythmia in the Female Heart via Alteration of Calcium Handling
There is wide-spread human exposure to bisphenol A (BPA), a ubiquitous estrogenic endocrine disruptor that has been implicated as having potentially harmful effects on human heart health. Higher urine BPA concentrations have been shown to be associated with cardiovascular diseases in humans. However, neither the nature nor the mechanism(s) of BPA action on the heart are understood. leak suppressed estrogen-induced triggered activities. The rapid response of female myocytes to estrogens was abolished in an estrogen receptor (ER) β knockout mouse model. leak. Our study provides the first experimental evidence suggesting that exposure to estrogenic endocrine disrupting chemicals and the unique sensitivity of female hearts to estrogens may play a role in arrhythmogenesis in the female heart
AMP-Activated Protein Kinase-Regulated Activation of the PGC-1Îą Promoter in Skeletal Muscle Cells
The mechanisms by which PGC-1Îą gene expression is controlled in skeletal muscle remains largely undefined. Thus, we sought to investigate the transcriptional regulation of PGC-1Îą using AICAR, an activator of AMPK, that is known to increase PGC-1Îą expression. A 2.2 kb fragment of the human PGC-1Îą promoter was cloned and sequence analysis revealed that this TATA-less sequence houses putative consensus sites including a GC-box, a CRE, several IRSs, a SRE, binding sites for GATA, MEF2, p 53, NF-ÎşB, and EBox binding proteins. AMPK activation for 24 hours increased PGC-1Îą promoter activity with concomitant increases in mRNA expression. The effect of AICAR on transcriptional activation was mediated by an overlapping GATA/EBox binding site at â495 within the PGC-1Îą promoter based on gel shift analyses that revealed increases in GATA/EBox DNA binding. Mutation of the EBox within the GATA/EBox binding site in the promoter reduced basal promoter activity and completely abolished the AICAR effect. Supershift analyses identified USF-1 as a DNA binding transcription factor potentially involved in regulating PGC-1Îą promoter activity, which was confirmed in vivo by ChIP. Overexpression of either GATA-4 or USF-1 alone increased the p851 PGC-1Îą promoter activity by 1.7- and 2.0-fold respectively, while co-expression of GATA-4 and USF-1 led to an additive increase in PGC-1Îą promoter activity. The USF-1-mediated increase in PGC-1Îą promoter activation led to similar increases at the mRNA level. Our data identify a novel AMPK-mediated regulatory pathway that regulates PGC-1Îą gene expression. This could represent a potential therapeutic target to control PGC-1Îą expression in skeletal muscle
Quantificação de fatores de crescimento na pele de equinos tratada com plasma rico em plaquetas
O plasma rico em plaquetas (PRP) ĂŠ um produto derivado da centrifugação do sangue total, sendo rico em fatores bioativos, como os de crescimento. Apesar da ampla utilização em processos cicatriciais, hĂĄ controvĂŠrsia sobre a eficĂĄcia da terapia na cicatrização cutânea. O objetivo desse estudo foi quantificar e comparar a concentração dos fatores TGF-β1 e PDGF-BB no PRP, plasma sanguĂneo e pele, durante diferentes fases do processo de cicatrização da pele tratada ou nĂŁo com PRP. Foram utilizados sete equinos machos castrados, mestiços, hĂgidos, com idade entre 16 e 17 (16,14Âą0,63) anos. TrĂŞs lesĂľes em formato quadrangular (6,25cm²) foram produzidas cirurgicamente nas regiĂľes glĂşteas direita e esquerda de todos os animais. Doze horas apĂłs indução das feridas, 0,5mL do PRP foi administrado em cada uma das quatro extremidades das feridas de uma das regiĂľes glĂşteas (Grupo tratado = GT), escolhida aleatoriamente. A regiĂŁo contralateral foi utilizada como controle (GC). As feridas foram submetidas Ă limpeza diĂĄria com ĂĄgua Milli Q, e amostras foram obtidas mediante biĂłpsias realizadas com Punch de 6mm. Foram obtidas seis biĂłpsias de pele, sendo a primeira realizada logo apĂłs a produção da ferida (T0), e as demais com 1 (T1) 2 (T2) 7 (T3) e 14 (T4) dias apĂłs a indução da lesĂŁo. A sexta biĂłpsia (T5) foi obtida apĂłs completo fechamento da pele, que ocorreu aproximadamente aos 37 dias (36,85Âą7,45, GC; 38,85Âą6,46, GT). TambĂŠm foram obtidas amostras de sangue com EDTA em todos os tempos mencionados. A quantificação dos fatores de crescimento TGF-β1 e PDGF-BB na pele, PRP e plasma sanguĂneo foi realizada pela tĂŠcnica ELISA. Os dados foram analisados estatisticamente pelo teste t, correlação de Pearson e regressĂŁo, utilizando nĂvel de significância de 5%. NĂŁo houve diferença entre os grupos, nos valores dos dois fatores de crescimento mensurados na pele, nos diferentes tempos. TambĂŠm nĂŁo houve correlação entre a quantidade dos fatores de crescimento presentes na pele e no plasma. Por outro lado, correlação positiva foi observada entre PRP e pele no grupo tratado, para os fatores de crescimento TGF-β1 (r=0,31) e PDGF-BB (r=0,38), bem como entre ambos os fatores de crescimento presentes no PRP (r=0,81). Considerando as concentraçþes dos fatores de crescimento no T0, os maiores valores cutâneos (p<0,05) do TGF-β1, em ambos os grupos, ocorreram nos tempos T3 e T5. Valores mais elevados (p<0,05) do PDGF-BB ocorreram no T4 (GT) e T5 (GC). No plasma nĂŁo houve alteração nas concentraçþes desses fatores em relação ao T0, o que sugere que o PRP nĂŁo acarreta efeito sistĂŞmico, quando os procedimentos adotados na presente pesquisa sĂŁo utilizados. A administração local de PRP no volume estudado, 12 h apĂłs indução cirĂşrgica de ferida cutânea na regiĂŁo glĂştea de equinos nĂŁo ocasiona maiores concentraçþes dos fatores de crescimento TGF-β1 e PDGF-BB no plasma sanguĂneo e pele, durante o processo de cicatrização
Targeting the renin-angiotensin-aldosterone system in fibrosis
Fibrosis is characterized by excessive deposition of extracellular matrix components such as collagen in tis-sues or organs. Fibrosis can develop in the heart, kidneys, liver, skin or any other body organ in response to injury or maladaptive reparative processes, reducing overall function and leading eventually to organ failure. A variety of cellular and molecular signaling mechanisms are involved in the pathogenesis of fibrosis. The renin-angiotensin-aldosterone system (RAAS) interacts with the potent Transforming Growth Factor beta (TGF beta) pro-fibrotic pathway to mediate fibrosis in many cell and tissue types. RAAS consists of both classical and alternative pathways, which act to potentiate or antagonize fibrotic signaling mechanisms, respectively. This review provides an overview of recent literature describing the roles of RAAS in the pathogenesis of fibrosis, particularly in the liver, heart, kidney and skin, and with a focus on RAAS interactions with TGF beta sig-naling. Targeting RAAS to combat fibrosis represents a promising therapeutic approach, particularly given the lack of strategies for treating fibrosis as its own entity, thus animal and clinical studies to examine the impact of natural and synthetic substances to alter RAAS signaling as a means to treat fibrosis are reviewed as well. (c) 2020 Elsevier B.V. All rights reserved.12 month embargo; published online 16 May 2020This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at [email protected]
Functional crosstalk of PGC-1 coactivators and inflammation in skeletal muscle pathophysiology
Skeletal muscle is an organ involved in whole body movement and energy metabolism with the ability to dynamically adapt to different states of (dis-)use. At a molecular level, the peroxisome proliferator-activated receptor Îł coactivators 1 (PGC-1s) are important mediators of oxidative metabolism in skeletal muscle and in other organs. Musculoskeletal disorders as well as obesity and its sequelae are associated with PGC-1 dysregulation in muscle with a concomitant local or systemic inflammatory reaction. In this review, we outline the function of PGC-1 coactivators in physiological and pathological conditions as well as the complex interplay of metabolic dysregulation and inflammation in obesity with special focus on skeletal muscle. We further put forward the hypothesis that, in this tissue, oxidative metabolism and inflammatory processes mutually antagonize each other. The nuclear factor ÎşB (NF-ÎşB) pathway thereby plays a key role in linking metabolic and inflammatory programs in muscle cells. We conclude this review with a perspective about the consequences of such a negative crosstalk on the immune system and the possibilities this opens for clinical applications