Utjecaj parenja na lomljenje oraha (Juglans regia L.)

The influence of hot steam vaporization of the walnut (Juglans regia L.) cultivar \u27Franquette\u27 on the required force for deformation (breaking) and the energy required to fracture the shell with the centrifugal breaking machine was studied under the laboratory conditions. The maximum force (248.04 N) was used for breaking nuts, which were vaporized for 5 minutes and 171.35 N for 15 minutes vaporized samples of nuts. The shortest deformation (1.3 mm) was measured for breaking 0 minute vaporized nuts, and the maximum deformation (4.15 mm) for 15 minutes vaporization samples. The largest percent of the whole kernels (74.62 %), was measured on 15 minutes vaporized nuts and the lowest on 0 minutes vaporized sample (28.47 %).U pokusu usporedbe morfoloških i fizikalnih svojstava ploda oraha (Juglans regia L.) sorte \u27Franquette\u27 proučavan je utjecaj parenja vručom parom na potrebnu silu za deformaciju (lomljenje) ploda te potrebnu energiju za lom ljuske ploda pomoću centrifugalnog stroja za lomljenja. Maksimalna sila (248,04 N) za lomljenje oraha izmjerena je kod 5 minuta parenih uzoraka, a najmanja kod 15 minuta parenja (171,35 N). Najmanja deformacija (1,3 mm) izmjerena je kod lomljenja suhih oraha, a najveća kod 15 minuta parenih (4,15 mm). Najveći dio celih jezgri (74,62 %), dobiven je kod lomljenja 15 minuta parenih oraha a najmanji kod 0 minuta parenja (28,47 %.)

Codi-strat - an interdisciplinary network geared towards sustainable management of chronic and infective diseases

A collaborative effort of clinicians, infectologists, molecular biologists, pharmacologists, veterinarians, bioinformaticians, management and education specialists is united in order to develop novel strategies of detecting early stages of chronic and infective diseases, their prevention and therapy. CODI-STRAT integrates 15 centers conducting leading–edge research of chronic inflammatory/infective diseases from seven European (five Mediterranean) countries and the USA, with specific aims to: i) establish long-standing partner center cross-disciplinary collaborations for clinical studies and research, ii) provide young investigators with broad and content-driven training and employability and iii) promote scientists up-skilled in genomics, transcriptomics, tissue expression, human serological and genetic studies, bioinformatics, chip technology, cell cultures and animal models, all directed toward clinical translation and chronic/infective disease management. This manuscript outlines the goals, partner roles and development of CODI-STRAT and its programme.peer-reviewe

Antibodies against carbonic anhydrase in patients with aplastic anemia

BACKGROUND/AIMS: Antibodies against carbonic anhydrase (CA) have been detected in patients with an aplastic anemia (AA)-like syndrome after autologous stem cell transplantation. METHODS: We analyzed sera of 53 bona fide AA patients before and after treatment with anti-thymocyte globulin (ATG) or bone marrow transplantation for the presence of anti-CA antibodies. RESULTS: Anti-CA antibodies were detected in 20 patients (38%) and were associated with older age at diagnosis of AA. Antibody-positive patients showed poor response to ATG treatment (complete response 14%) and inferior long-term survival (36% at 10 years), when compared to antibody-negative patients (complete response and 10-year survival both 64%). Two thirds of patients with antibodies at diagnosis of AA became antibody negative after treatment with ATG. Clearance of the antibody did not appear to be associated with hematological improvement. CONCLUSION: Antibodies against CA are detected frequently at diagnosis of AA, and their presence identifies a subset of patients with poor response to immunosuppressive treatment

Overlap of epitopes recognized by anti-carbonic anhydrase I IgG in patients with malignancy-related aplastic anemia-like syndrome and in patients with aplastic anemia

High titers of anti-carbonic anhydrase I (anti-CA I) autoantibodies were detected in the sera of patients with malignancies who developed an aplastic anemia-like (AA-like) syndrome after a high-dose therapy (HDT) and autologous stem cell transplantation (ASCT). It was found, that the presence of these anti-CA I autoantibodies is associated with spontaneous tumor regression. The main immunodominant epitopes of carbonic anhydrase isoform I (CA I) have previously been identified using epitope extraction technique in combination with mass spectrometric detection and bioinformatic verification. Similarly, the sera of patients with bona fide aplastic anemia (AA) who poorly responded to immunosuppressive treatment with anti-thymocyte globulin (ATG) demonstrated high titers of anti-CA I antibodies. In order to reveal differences between these antibodies, we applied the same methodology of epitope mapping procedure. Surprisingly, the anti-CA I antibodies from the both groups of patients compatibly recognized the same four candidate CA I epitopes--DGLAV, NVGHS, SLKPI, SSEQL. This finding may indicate common pathophysiological mechanisms in these two syndromes. However, at this moment it remains unresolved if anti-CA I antibodies are implicated in marrow or tumor suppression or are just an epi-phenomenon

High Avidity Anti-β2-Glycoprotein i Antibodies Activate Human Coronary Artery Endothelial Cells and Trigger Peripheral Blood Mononuclear Cell Migration

Anti-β2-glycoprotein I antibodies (aβ2GPI) represent a potential pathogenic candidate for coronary artery diseases. High avidity aβ2GPI (HAv aβ2GPI) are known to be associated with thrombotic and obstetric manifestations in patients with antiphospholipid syndrome, who are also susceptible to the development of premature atherosclerosis. However, there is little information about how human coronary artery endothelial cells (HCAEC) are affected by HAv aβ2GPI. The purpose of our study was to evaluate the pathophysiological effects of HAv aβ2GPI on HCAEC and determine their influence on cytokine expression and migration of peripheral blood mononuclear cells. Following the two hit hypothesis, we co-stimulated HAv aβ2GPI-treated HCAEC in the presence and absence of the acute phase protein serum amyloid A (SAA). HAv aβ2GPI induced in vitro HCAEC dysfunction, through the ERK1/2 signaling pathway, promoted the expression of chemokines (MCP-1, GROα and IL-8) and IL-6, which led to the attraction and migration of peripheral blood mononuclear cells. These effects were potentiated and intensified in conditions with SAA, indicating that HAv aβ2GPI, in the presence of physiological concentrations of acute-phase proteins represent pathogenic autoantibodies, which could lead to the development of premature atherosclerosis and/or thrombosis development

A significance of additional chromosomal aberrations and other variables on post transplantation outcome of patients with CML

Chronic myeloic leukemia (CML) is a malignant disease of hematopoietic stem cell characterized by the bcr/abl gene rearrangement. Allogeneic transplantation of stem cells (SCT) is a routinely used treatment method of patients with this diagnosis and remains the only curative mode of treatment. From January 1990 to December 2002, 78 patients with CML underwent allogeneic transplantation and were examined at the Department of Genetics in the National Cancer Institute in Bratislava. Using conventional cytogenetic and FISH 6 patients (7.7%) showed additional chromosomal changes before SCT. These patients had statistically worse post transplantation prognosis compared to the patients without additional changes before SCT (mean survival in month+/-standard error (58.08 (+/-6.70) vs. 5.17 (+/-0.98), p-value=0.001), patient mortality (67% vs. 31%)). In addition five other variables were evaluated for transplant outcome, namely, patient's age at the time of transplantation, sibling or non-sibling donor, higher than 1st chronic phase CML, time from diagnosis to transplantation and sex of donor and recipient. Only the comparison of HLA-identical sibling transplantation to unrelated donor transplantation was statistically significant (mean survival in month- 56.6 (+/-7.2) vs. 13 (+/-0.0), patient mortality 31% vs. 67%)

Development and validation of the solution-focused inventory

Solution-focused coaching and solution-focused therapy are strengths-based approaches which emphasize people\u27s resources and resilience and how these can be used in the pursuit of purposeful, positive change. The Solution-focused Inventory (SFI) is a 12-item scale with three subscales: Problem Disengagement, Goal Orientation and Resource Activation. Three studies in this article provide support for the validity of the SFI as a measure of solution-focused thinking. The SFI negatively correlated with psychopathology and positively correlated with measures of well-being, resilience and perspective taking. Test-retest reliability over 16 weeks was 0.84. Cronbach\u27s alpha for the 12-item scale was 0.84. It also demonstrates sensitivity to purposeful change in that participation in a leadership development coaching intervention was associated with significantly increased scores on the SFI, whilst scores for the control group did not change