Cozymase. A study of purification methods

Cozymase is one of the essential components of the complex enzyme mixture which effects alcoholic fermentation in the absence of living cells. The separation of the mixture into zymase and cozymase was first accomplished by Harden and Young [1] by means of ultrafiltration through a gelatin-impregnated Chamberland filter candle. The residue and filtrate as thus prepared possessed, separately, no fermentative action, but when mixed were found to produce a rapid fermentation. The active constituent of the residue was named zymase, while that constituent of the filtrate responsible for the reactivation of the residue was named cozymase. We studied the purification produced in our material by a variety of reagents. In the investigation we have repeated much of the work done by von Euler and Myrbäk [2], and several differences have been found, which appear difficult to explain solely upon the basis of the lower initial purity of our material. As certain of the experiments show distinct promise, we hope to be able to extend the work upon a material of considerably higher original purity, such as was employed by von Euler and Myrbäk

Bose-Einstein condensation in complex networks

The evolution of many complex systems, including the world wide web, business and citation networks is encoded in the dynamic web describing the interactions between the system's constituents. Despite their irreversible and non-equilibrium nature these networks follow Bose statistics and can undergo Bose-Einstein condensation. Addressing the dynamical properties of these non-equilibrium systems within the framework of equilibrium quantum gases predicts that the 'first-mover-advantage', 'fit-get-rich' and 'winner-takes-all' phenomena observed in competitive systems are thermodynamically distinct phases of the underlying evolving networks

Impurity transport in a mixed-collisionality stellarator plasma

A potential threat to the performance of magnetically confined fusion plasmas is the problem of impurity accumulation, which causes the concentration of highly charged impurity ions to rise uncontrollably in the center of the plasma and spoil the energy confinement by excessive radiation. It has long been thought that the collisional transport of impurities in stellarators always leads to such accumulation (if the electric field points inwards, which is usually the case), whereas tokamaks, being axisymmetric, can benefit from "temperature screening", i.e., an outward flux of impurities driven by the temperature gradient. Here it is shown, using analytical techniques supported by results from a new numerical code, that such screening can arise in stellarator plasmas too, and indeed does so in one of the most relevant operating regimes, where the impurities are highly collisional whilst the bulk plasma is in any of the low-collisionality regimes.Comment: 11 pages, 3 figure

Impurity transport and bulk ion flow in a mixed collisionality stellarator plasma

The accumulation of impurities in the core of magnetically confined plasmas, resulting from standard collisional transport mechanisms, is a known threat to their performance as fusion energy sources. Whilst the axisymmetric tokamak systems have been shown to benefit from the effect of temperature screening, that is an outward flux of impurities driven by the temperature gradient, impurity accumulation in stellarators was thought to be inevitable, driven robustly by the inward pointing electric field characteristic of hot fusion plasmas. We have shown in Helander et. al. (2017b) that such screening can in principle also appear in stellarators, in the experimentally relevant mixed collisionality regime, where a highly collisional impurity species is present in a low collisionality bulk plasma. Details of the analytic calculation are presented here, along with the effect of the impurity on the bulk ion flow, which will ultimately affect the bulk contribution to the bootstrap current

Recent Developments in Osteogenesis Imperfecta

Osteogenesis imperfecta (OI) is an uncommon genetic bone disease associated with brittle bones and fractures in children and adults. Although OI is most commonly associated with mutations of the genes for type I collagen, many other genes (some associated with type I collagen processing) have now been identified. The genetics of OI and advances in our understanding of the biomechanical properties of OI bone are reviewed in this article. Treatment includes physiotherapy, fall prevention, and sometimes orthopedic procedures. In this brief review, we will also discuss current understanding of pharmacologic therapies for treatment of OI

A type system for components

In modern distributed systems, dynamic reconfiguration, i.e., changing at runtime the communication pattern of a program, is chal- lenging. Generally, it is difficult to guarantee that such modifications will not disrupt ongoing computations. In a previous paper, a solution to this problem was proposed by extending the object-oriented language ABS with a component model allowing the programmer to: i) perform up- dates on objects by means of communication ports and their rebinding; and ii) precisely specify when such updates can safely occur in an object by means of critical sections. However, improper rebind operations could still occur and lead to runtime errors. The present paper introduces a type system for this component model that extends the ABS type system with the notion of ports and a precise analysis that statically enforces that no object will attempt illegal rebinding

Topology of evolving networks: local events and universality

Networks grow and evolve by local events, such as the addition of new nodes and links, or rewiring of links from one node to another. We show that depending on the frequency of these processes two topologically different networks can emerge, the connectivity distribution following either a generalized power-law or an exponential. We propose a continuum theory that predicts these two regimes as well as the scaling function and the exponents, in good agreement with the numerical results. Finally, we use the obtained predictions to fit the connectivity distribution of the network describing the professional links between movie actors.Comment: 13 pages, 3 figure

Constraining blazar distances with combined Fermi and TeV data: an empirical approach

We discuss a method to constrain the distance of blazars with unknown redshift using combined observations in the GeV and TeV regimes. We assume that the VHE spectrum corrected for the absorption through the interaction with the Extragalactic Background Light can not be harder than the spectrum in the Fermi/LAT band. Starting from the observed VHE spectral data we derive the EBL-corrected spectra as a function of the redshift z and fit them with power laws to be compared with power law fits to the LAT data. We apply the method to all TeV blazars detected by LAT with known distance and derive an empirical law describing the relation between the upper limits and the true redshifts that can be used to estimate the distance of unknown redshift blazars. Using different EBL models leads to systematic changes in the derived upper limits. Finally, we use this relation to infer the distance of the unknown redshift blazar PKS 1424+240.Comment: 5 pages, 4 figures, accepted for publication in MNRAS, minor revisio

Giant strongly connected component of directed networks

We describe how to calculate the sizes of all giant connected components of a directed graph, including the {\em strongly} connected one. Just to the class of directed networks, in particular, belongs the World Wide Web. The results are obtained for graphs with statistically uncorrelated vertices and an arbitrary joint in,out-degree distribution $P(k_i,k_o)$. We show that if $P(k_i,k_o)$ does not factorize, the relative size of the giant strongly connected component deviates from the product of the relative sizes of the giant in- and out-components. The calculations of the relative sizes of all the giant components are demonstrated using the simplest examples. We explain that the giant strongly connected component may be less resilient to random damage than the giant weakly connected one.Comment: 4 pages revtex, 4 figure