Influência do tempo de condução de agroflorestas na atividade microbiológica do solo.

A atividade biológica é um ótimo indicador de qualidade do solo, pois é um atributo sensível ás características bióticas e abióticas do sistema. A fim de quantificar esses atributos, foram coletadas amostras de solo nas profundidades de 0 a 2,5 cm, 2,5 a 5 cm, 5 a 10 cm, 10 a 15 cm, 15 a 30 cm, 30 a 45 cm e 45 a 60 cm para obtenção do carbono da biomassa microbiana (BMS-C), respiração basal do solo (RBS) e quociente metabólico (qCO2) em áreas de regeneração natural de 10 anos e agroflorestas de 5 e 10 anos. As áreas de regeneração natural apresentaram teores mais elevados de BMS-C e maior RBS, porém maiores qCO2 foram encontrados nas agroflorestas de 10 anos devido ao manejo utilizado nas agroflorestas.Resumo expandido

A prospective investigation of interleukin-8 levels in pediatric acute respiratory failure and acute respiratory distress syndrome

© 2019 The Author(s). Background: The association of plasma interleukin-8 (IL-8), or IL-8 genetic variants, with pediatric acute respiratory distress syndrome (PARDS) in children with acute respiratory failure at risk for PARDS has not been examined. The purpose of this study was to examine the association of early and sequential measurement of plasma IL-8 and/or its genetic variants with development of PARDS and other clinical outcomes in mechanically ventilated children with acute respiratory failure. Methods: This was a prospective cohort study of children 2 weeks to 17 years of age with acute airways and/or parenchymal lung disease done in 22 pediatric intensive care units participating in the multi-center clinical trial, Randomized Evaluation of Sedation Titration for Respiratory Failure (RESTORE). Plasma IL-8 levels were measured within 24 h of consent and 24 and 48 h later. DNA was purified from whole blood, and IL-8 single nucleotide polymorphisms, rs4073, rs2227306, and rs2227307, were genotyped. Results: Five hundred forty-nine patients were enrolled; 480 had blood sampling. Plasma IL-8 levels ranged widely from 4 to 7373 pg/mL. Highest IL-8 levels were observed on the day of intubation with subsequent tapering. Levels of IL-8 varied significantly across primary diagnoses with the highest levels occurring in patients with sepsis and the lowest levels in those with asthma. Plasma IL-8 was strongly correlated with oxygenation defect and severity of illness. IL-8 was consistently higher in PARDS patients compared to those without PARDS; levels were 4-12 fold higher in non-survivors compared to survivors. On multivariable analysis, IL-8 was independently associated with death, duration of mechanical ventilation, and PICU length of stay on all days measured, but was not associated with PARDS development. There was no association between the IL-8 single nucleotide polymorphisms, rs4073, rs2227306, and rs2227307, and PARDS development or plasma IL-8 level. Conclusions: When measured sequentially, plasma IL-8 was robustly associated with multiple, relevant clinical outcomes including mortality, but was not associated with PARDS development. The wide range of plasma IL-8 levels exhibited in this cohort suggests that further study into the heterogeneity of this patient population and its impact on individual responses to PARDS treatment is warranted

Summary of Methodology Used in Enterotoxigenic Escherichia coli (ETEC) Challenge Experiments in Weanling Pigs and Quantitative Assessment of Observed Variability

Post-weaning diarrhea in pigs can be caused by the F4 or F18 strains of enterotoxigenic Escherichia coli (ETEC). To evaluate interventions for ETEC, experimental infection via a challenge model is critical. To our knowledge, there is a lack of explanation for the variability in responses observed across ETEC challenge studies. Our objective was to quantitatively summarize the responses and variability among recent ETEC challenge studies and develop a tool for sample size calculation. The most widely evaluated response criteria across ETEC challenge studies are growth performance, fecal consistency and bacterial shedding, intestinal morphology, and immune responses. Factors that contribute to the variability seen across studies include the type of ETEC studied, dose and timing of inoculation, and the number of replications. Generally, a reduction in average daily gain (ADG) and average daily feed intake (ADFI) are seen following an ETEC challenge, as well as a rapid increase in diarrhea. Fecal bacterial shedding is a common indicator of ETEC infection, but the responses seen across the literature are not consistent due to differences in bacterial enumeration procedures. Emphasis should also be placed on the piglet’s immune response to ETEC, which is commonly assessed by quantifying levels of immunoglobulins and pro-inflammatory cytokines. Again, there is variability in these responses across published work. Small intestinal morphology is drastically altered following infection with ETEC and appears to be a less variable response criterion to evaluate. While there is a large degree of variability across ETEC challenge experiments, we have provided a quantitative summary of these studies, and a Microsoft Excel-based tool was created to help calculate sample sizes for future studies

Understanding clinical and biological heterogeneity to advance precision medicine in paediatric acute respiratory distress syndrome

Paediatric acute respiratory distress syndrome (PARDS) is a heterogeneous clinical syndrome that is associated with high rates of mortality and long-term morbidity. Factors that distinguish PARDS from adult acute respiratory distress syndrome (ARDS) include changes in developmental stage and lung maturation with age, precipitating factors, and comorbidities. No specific treatment is available for PARDS and management is largely supportive, but methods to identify patients who would benefit from specific ventilation strategies or ancillary treatments, such as prone positioning, are needed. Understanding of the clinical and biological heterogeneity of PARDS, and of differences in clinical features and clinical course, pathobiology, response to treatment, and outcomes between PARDS and adult ARDS, will be key to the development of novel preventive and therapeutic strategies and a precision medicine approach to care. Studies in which clinical, biomarker, and transcriptomic data, as well as informatics, are used to unpack the biological and phenotypic heterogeneity of PARDS, and implementation of methods to better identify patients with PARDS, including methods to rapidly identify subphenotypes and endotypes at the point of care, will drive progress on the path to precision medicine.</p

Impacts of a Post-Transport/Pre-Processing Rest Period on the Growth Performance and Serum Metabolites of Cattle Entering a Feedlot

Objective: The objective of this study was to evaluate the impact of a post-transport rest period on receiving calf growth performance and blood serum metabolites as indicators of immune function. Study Description: Eighty heifers were purchased from a sale barn and transported 6 hours to the Kansas State University Beef Cattle Research Center where they were processed at one of four times: immediately upon arrival or after a 6-, 24-, or 48-hour rest period. Cattle were then fed for 35 days with growth performance data collected weekly. Blood samples were also collected and analyzed for serum infectious bovine rhinotracheitis (IBR) titer and biochemical parameters. Results: Processing time did not impact (P \u3e 0.05) heifer average daily gain. Overall, dry matter intake (DMI) decreased linearly (P = 0.027) as the rest time increased. The number of days for heifers to reach a targeted DMI of 2.5% body weight was linearly increased (P = 0.023) as time of rest increased. Serum IBR titer for heifers processed at either 0 or 6 hours upon arrival was higher (P \u3c 0.01) on day 35 compared to day 0. This response was expected, as these cattle were vaccinated immediately or shortly after arrival. Interestingly, no difference in IBR titer was observed (P \u3e 0.05) between day 0 and day 35 for heifers processed at either 24 or 48 hours upon arrival, indicating potential seroconversion of IBR antibodies before vaccination. The Bottom Line: These results indicate that rest time after arrival and prior to processing may not affect calf growth performance, but there is evidence that a 6-hour rest period could maximize DMI upon arrival to a feedlot. Additional research with greater replication and more industry-standard experimental conditions should be conducted to further evaluate these parameters

Early Use of Adjunctive Therapies for Pediatric Acute Respiratory Distress Syndrome:A PARDIE Study

Rationale: Few data exist to guide early adjunctive therapy use in pediatric acute respiratory distress syndrome (PARDS). Objectives: To describe contemporary use of adjunctive therapies for early PARDS as a framework for future investigations. Methods: This was a preplanned substudy of a prospective, international, cross-sectional observational study of children with PARDS from 100 centers over 10 study weeks. Measurements and Main Results: We investigated six adjunctive therapies for PARDS: continuous neuromuscular blockade, corticosteroids, inhaled nitric oxide (iNO), prone positioning, high-frequency oscillatory ventilation (HFOV), and extracorporeal membrane oxygenation. Almost half (45%) of children with PARDS received at least one therapy. Variability was noted in the median starting oxygenation index of each therapy; corticosteroids started at the lowest oxygenation index (13.0; interquartile range, 7.6–22.0) and HFOV at the highest (25.7; interquartile range, 16.7–37.3). Continuous neuromuscular blockade was the most common, used in 31%, followed by iNO (13%), corticosteroids (10%), prone positioning (10%), HFOV (9%), and extracorporeal membrane oxygenation (3%). Steroids, iNO, and HFOV were associated with comorbidities. Prone positioning and HFOV were more common in middle-income countries and less frequently used in North America. The use of multiple ancillary therapies increased over the first 3 days of PARDS, but there was not an easily identifiable pattern of combination or order of use. Conclusions: The contemporary description of prevalence, combinations of therapies, and oxygenation threshold for which the therapies are applied is important for design of future studies. Region of the world, income, and comorbidities influence adjunctive therapy use and are important variables to include in PARDS investigations

Serial killing and the postmodern self

© 2006 by SAGE PublicationsThe self has been a consistently central theme in philosophy and the social sciences and, in the last decades of the 20th century, the fragmentation of the modern self has engendered extensive academic commentary. In order to contribute to current discussions about self, it is perhaps most effective to map the transformation of a single representation of the self in contemporary culture. As a cultural ‘flashpoint’, the serial killer could provide an apposite analytical focus. Drawing critically on Mark Seltzer's work on serial killers this article interprets serial killing as a form of commodified transgression. In contrast to the modern self, established through state-institutionalized routines, serial killers establish their identities through ecstatic intercourse. These acts of bodily and ethical transgression are facilitated by the use of commodities. In this way, the serial killer represents a self which is consistent with the colonization of interpersonal relations by multinational capital. The serial killer signifies the appearance of a postmodern self