5,632 research outputs found

    Positive regulation of meiotic DNA double-strand break formation by activation of the DNA damage checkpoint kinase Mec1(ATR)

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    During meiosis, formation and repair of programmed DNA double-strand breaks (DSBs) create genetic exchange between homologous chromosomes-a process that is critical for reductional meiotic chromosome segregation and the production of genetically diverse sexually reproducing populations. Meiotic DSB formation is a complex process, requiring numerous proteins, of which Spo11 is the evolutionarily conserved catalytic subunit. Precisely how Spo11 and its accessory proteins function or are regulated is unclear. Here, we use Saccharomyces cerevisiae to reveal that meiotic DSB formation is modulated by the Mec1(ATR) branch of the DNA damage signalling cascade, promoting DSB formation when Spo11-mediated catalysis is compromised. Activation of the positive feedback pathway correlates with the formation of single-stranded DNA (ssDNA) recombination intermediates and activation of the downstream kinase, Mek1. We show that the requirement for checkpoint activation can be rescued by prolonging meiotic prophase by deleting the NDT80 transcription factor, and that even transient prophase arrest caused by Ndt80 depletion is sufficient to restore meiotic spore viability in checkpoint mutants. Our observations are unexpected given recent reports that the complementary kinase pathway Tel1(ATM) acts to inhibit DSB formation. We propose that such antagonistic regulation of DSB formation by Mec1 and Tel1 creates a regulatory mechanism, where the absolute frequency of DSBs is maintained at a level optimal for genetic exchange and efficient chromosome segregation

    Improvement and further development of SSM/I overland parameter algorithms using the WetNet workstation

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    Since the launch of the DMSP Special Sensor Microwave/Imager (SSM/I), several algorithms have been developed to retrieve overland parameters. These include the present operational algorithms resulting from the Navy calibration/validation effort such as land surface type (Neale et al. 1990), land surface temperature (McFarland et al. 1990), surface moisture (McFarland and Neale, 1991) and snow parameters (McFarland and Neale, 1991). In addition, other work has been done including the classification of snow cover and precipitation using the SSM/I (Grody, 1991). Due to the empirical nature of most of the above mentioned algorithms, further research is warranted and improvements can probably be obtained through a combination of radiative transfer modelling to study the physical processes governing the microwave emissions at the SSM/I frequencies, and the incorporation of additional ground truth data and special cases into the regression data sets. We have proposed specifically to improve the retrieval of surface moisture and snow parameters using the WetNet SSM/I data sets along with ground truth information namely climatic variables from the NOAA cooperative network of weather stations as well as imagery from other satellite sensors such as the AVHRR and Thematic Mapper. In the case of surface moisture retrievals the characterization of vegetation density is of primary concern. The higher spatial resolution satellite imagery collected at concurrent periods will be used to characterize vegetation types and amounts which, along with radiative transfer modelling should lead to more physically based retrievals. Snow parameter retrieval algorithm improvement will initially concentrate on the classification of snowpacks (dry snow, wet snow, refrozen snow) and later on specific products such as snow water equivalent. Significant accomplishments in the past year are presented

    Comparison of eleven vegetation indices for estimating plant height of alfalfa and grass

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    Estimating soil heat flux for alfalfa and clipped tall fescue grass

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    Evaluation of a hybrid remote sensing evapotranspiration model for variable rate irrigation management

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    Accurate generation of spatial irrigation prescriptions is essential for implementation and evaluation of variable rate irrigation (VRI) technology. A hybrid remote sensing evapotranspiration (ET) model was evaluated for use in developing irrigation prescriptions for a VRI center pivot. The model is a combination of a two-source energy balance model and a reflectance based crop coefficient water balance model. Spatial ET and soil water depletion were modeled for a 10 km2 area consisting of rainfed and irrigated maize fields in eastern Nebraska for 2013. Multispectral images from Landsat 8 Operational Land Imager and Thermal Infrared Sensor were used as model input. Modeled net radiation and soil heat fluxes compared well with measurements from eddy covariance systems located within three fields in the study area. Modeled sensible heat flux did not compare well. Latent heat flux compared well for the only mid-summer image, but poorly for the one spring and two fall images. The water balance ET compared well with the two-source energy balance ET for irrigated maize, but not for dryland maize. Image frequency is thought to be a contributing factor in the poor performance of the water balance. In 2015 the hybrid model will be used to generate irrigation prescription maps for a VRI system located in the study area based on modeled soil moisture depletion. Future research will focus on model parameterization and utilize aerial imagery and satellite imagery from other sensors for improved image frequency. Note: this is a revision of the original paper correcting erroneous data where one of the flux sites was mistakenly analyzed as soybeans, when it was actually maize. Mean biased error signs have also been corrected

    Events, processes, and the time of a killing

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    The paper proposes a novel solution to the problem of the time of a killing (ToK), which persistently besets theories of act-individuation. The solution proposed claims to expose a crucial wrong-headed assumption in the debate, according to which ToK is essentially a problem of locating some event that corresponds to the killing. The alternative proposal put forward here turns on recognizing a separate category of dynamic occurents, viz. processes. The paper does not aim to mount a comprehensive defense of process ontology, relying instead on extant defenses. The primary aim is rather to put process ontology to work in diagnosing the current state of play over ToK, and indeed in solving it

    Guidelines for validating Bowen ratio data

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    For a variety of reasons, the measurement of latent heat flux using the Bowen ratio method can sometimes result in erroneous data. This study provides guidelines for detecting erroneous Bowen ratio data and illustrates the application of these guidelines by comparing Bowen ratio and lysimeter data collected over grass and alfalfa in southern Idaho. Errors in net radiation were detected by comparing measured with theoretical values. However, it was found that good theoretical procedures to validate soil heat flux data are lacking. Only empirical equations mainly used for remote sensing applications to obtain estimates close to noontime are available. Extremely inaccurate latent heat fluxes were easily filtered out by rejecting data when the calculated Bowen ratio (Ξ²) values were close to -1. A simplified procedure was proposed to reject fluxes with the wrong sign, and three different equations were used successfully to detect the occurrence of condensation inside the type of measurement system used in the study. Guidelines to assure adequate fetch are provided. Fetch did not affect the measured fluxes in this study, which may have been due to the similarity in surface properties between the crops under study and those in the surrounding fields

    Evaluation of a Hybrid Reflectance-Based Crop Coefficient and Energy Balance Evapotranspiration Model for Irrigation Management

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    Accurate generation of spatial soil water maps is useful for many types of irrigation management. A hybrid remote sensing evapotranspiration (ET) model combining reflectance-based basal crop coefficients (Kcbrf) and a two-source energy balance (TSEB) model was modified and validated for use in real-time irrigation management. We modeled spatial ET for maize and soybean fields in eastern Nebraska for the 2011-2013 growing seasons. We used Landsat 5, 7, and 8 imagery as remote sensing inputs. In the TSEB, we used the Priestly-Taylor (PT) approximation for canopy latent heat flux, as in the original model formulations. We also used the Penman-Monteith (PM) approximation for comparison. We compared energy balance fluxes and computed ET with measurements from three eddy covariance systems within the study area. Net radiation was underestimated by the model when data from a local weather station were used as input, with mean bias error (MBE) of -33.8 to -40.9 W m-2. The measured incident solar radiation appeared to be biased low. The net radiation model performed more satisfactorily when data from the eddy covariance flux towers were input into the model, with MBE of 5.3 to 11.2 W m-2. We removed bias in the daily energy balance ET using a dimensionless multiplier that ranged from 0.89 to 0.99. The bias-corrected TSEB ET, using weather data from a local weather station and with local ground data in thermal infrared imagery corrections, had MBE = 0.09 mm d-1 (RMSE = 1.49 mm d-1) for PM and MBE = 0.04 mm d-1 (RMSE = 1.18 mm d-1) for PT. The hybrid model used statistical interpolation to combine the two ET estimates. We computed weighting factors for statistical interpolation to be 0.37 to 0.50 for the PM method and 0.56 to 0.64 for the PT method. Provisions were added to the model, including a real-time crop coefficient methodology, which allowed seasonal crop coefficients to be computed with relatively few remote sensing images. This methodology performed well when compared to basal crop coefficients computed using a full season of input imagery. Water balance ET compared favorably with the eddy covariance data after incorporating the TSEB ET. For a validation dataset, the magnitude of MBE decreased from -0.86 mm d-1 (RMSE = 1.37 mm d-1) for the Kcbrfalone to -0.45 mm d-1 (RMSE = 0.98 mm d-1) and -0.39 mm d-1 (RMSE = 0.95 mm d-1) with incorporation of the TSEB ET using the PM and PT methods, respectively. However, the magnitudes of MBE and RMSE were increased for a running average of daily computations in the full May-October periods. The hybrid model did not necessarily result in improved model performance. However, the water balance model is adaptable for real-time irrigation scheduling and may be combined with forecasted reference ET, although the low temporal frequency of satellite imagery is expected to be a challenge in real-time irrigation management
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