Kentucky Municipal Wastewater Treatment Plant Sludge Survey

A survey of solids removal from municipal wastewater treatment plants in Kentucky was conducted by the Department of Civil Engineering at the University of Kentucky. The survey was made using both a mailed form and a followed up phone communication through a period from June, 1993 to April, 1994. The results of that survey were compiled using a spreadsheet program and are available on diskette from the authors. The results of the survey are also presented in the eight Tables herein

Closed Universes With Black Holes But No Event Horizons As a Solution to the Black Hole Information Problem

We show it is possible for the information paradox in black hole evaporation to be resolved classically. Using standard junction conditions, we attach the general closed spherically symmetric dust metric to a spacetime satisfying all standard energy conditions but with a single point future c-boundary. The resulting Omega Point spacetime, which has NO event horizons, nevertheless has black hole type trapped surfaces and hence black holes. But since there are no event horizons, information eventually escapes from the black holes. We show that a scalar quintessence field with an appropriate exponential potential near the final singularity would give rise to an Omega Point final singularity.Comment: 27 pages in LaTex2e, no figure

Kentucky Municipal wastewater Sludge: Generation, Management and Pathogen Reduction

This document was prepared as part of an investigation into sludge quantities and pathogen reduction. It has been written as an introduction and reference for operators, municipal officials, engineers and regulators as they assess their sludge management options

Municipal Wastewater Sludges: Solids Generation and Pathogen Reduction

The qualities of sludge generated and the variations in potential processing technologies for pathogen reduction pose a challenge to those evaluating sludge management. Some of the key factors that should be considered when evaluating sludge management options include: Land requirements Equipment requirements Availability of Required additives Desired product end use The extent to which these factors influence the implementation of a particular processing technology will vary, but in all cases, they will influence the cost and application of any of the technologies

Quantitative Microbiology of the Scalp in Non-Dandruff, Dandruff, and Seborrheic Dermatitis

The composition of the scalp microflora was assessed quantitatively in normal individuals and in patients with dandruff and seborrheic dermatitis, disorders characterized by increasing scalin. Three organisms were constantly found: (1) Pityrosporum, (2) aerobic cocci, and (3) Corynebacterium acnes. Pitrosporum (mainly Pityrosporum ovale) made up 46% of the total microflora in normals, 74% in dandruff, and 83% in seborrheic dermatitis. The geometric mean number of organisms per cm2 in non-dandruff subjects was 5.04 × 105; 9.22 × 105 in dandruff subjects; and 6.45 × 105 in those with seborrheic dermatitis. The cocci were dominantly Baird-Parkertype SII and no quantitative or qualitative change occurred in the scaling disorders. C. acnes comprised 26% of the flora on the normal scalp, 6% in dandruff, and only 1% in seborrheic dermatitis. These results differ significantly from previous reports which describe a much more complex microflora and suggest an etiologic role for microorganisms in dandruff

Variability of walking in able-bodied adults across different time intervals

This study aimed to quantify the variability of walking in young adults across measurement sessions held at varied time intervals. Inconsistent marker placement, a major source of variation in gait measurement, was minimized in this study in order to quantify how much variation is attributable to participants naturally altering their walking. Three-dimensional gait data were captured from five young adults on four sessions on each of four days. After Day 1, marker locations were identified with permanent pen to minimise variance due to inconsistent marker placement. A multi-level linear regression model was used to estimate intertrial and inter-session variance for two hour, within-day, across-a-day and across-a-week intervals. Inter-trial variation was relatively constant within sessions and ranged from a standard deviation (SD) of 0.7 degrees to 2.5 degrees. Inter-session variation differed across gait variables and time intervals, with a maximum variation of 2.4 degrees (hip rotation, across a week). Young adults varied their kinematic walking patterns (SD = 1-2 degrees) over intervals of 2 hours to 1 week. In reliability studies, variations of this magnitude may simply reflect natural or ‘intrinsic’ human variation rather than marker placement error

Autocrine Production of IGF‐I Increases Stem Cell‐Mediated Neuroprotection

Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder resulting in motor neuron (MN) loss. There are currently no effective therapies; however, cellular therapies using neural progenitor cells protect MNs and attenuate disease progression in G93A‐SOD1 ALS rats. Recently, we completed a phase I clinical trial examining intraspinal human spinal stem cell (HSSC) transplantation in ALS patients which demonstrated our approach was safe and feasible, supporting the phase II trial currently in progress. In parallel, efforts focused on understanding the mechanisms underlying the preclinical benefit of HSSCs in vitro and in animal models of ALS led us to investigate how insulin‐like growth factor‐I (IGF‐I) production contributes to cellular therapy neuroprotection. IGF‐I is a potent growth factor with proven efficacy in preclinical ALS studies, and we contend that autocrine IGF‐I production may enhance the salutary effects of HSSCs. By comparing the biological properties of HSSCs to HSSCs expressing sixfold higher levels of IGF‐I, we demonstrate that IGF‐I production augments the production of glial‐derived neurotrophic factor and accelerates neurite outgrowth without adversely affecting HSSC proliferation or terminal differentiation. Furthermore, we demonstrate that increased IGF‐I induces more potent MN protection from excitotoxicity via both indirect and direct mechanisms, as demonstrated using hanging inserts with primary MNs or by culturing with organotypic spinal cord slices, respectively. These findings support our theory that combining autocrine growth factor production with HSSC transplantation may offer a novel means to achieve additive neuroprotection in ALS. Stem Cells 2015;33:1480–1489Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/111155/1/stem1933.pd

Sorption phenomena in subsurface systems: Concepts, models and effects on contaminant fate and transport

The behavior, transport and ultimate fate of contaminants in subsurface environments may be affected significantly by their participation in sorption reactions and related phenomena. The degree to which the resulting effects can be quantified and predicted depends upon the extent to which certain fundamental aspects of sorption are understood, and upon the accuracy with which these phenomena can be characterized and modeled in complex subsurface systems. Current levels of understanding of the reactions and processes comprising sorption phenomena are discussed in this paper, as are the forms and utilities of different models used to describe them. Emphasis is placed on concept development, on the translation of these concepts into functional models for characterizing sorption rates and equilibria, and on the application of these concepts and models for explaining contaminant behavior in subsurface systems. Examples are provided to illustrate the impacts of sorption phenomena on contaminant transport.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/29367/1/0000437.pd

The Secondary Bile acids, Ursodeoxycholic acid and Lithocholic Acid, Protect Against Intestinal Inflammation by Inhibition of Epithelial Apoptosis

Increased epithelial permeability is a key feature of IBD pathogenesis and it has been proposed that agents which promote barrier function may be of therapeutic benefit. We have previously reported the secondary bile acid, ursodeoxycholic acid (UDCA), to be protective in a mouse model of colonic inflammation and that its bacterial metabolism is required for its beneficial effects. The current study aimed to compare the effects of UDCA, LCA, and a non-metabolizable analog of UDCA, 6-methyl-UDCA (6-MUDCA), on colonic barrier function and mucosal inflammation in a mouse model of colonic inflammation. Bile acids were administered daily to C57Bl6 mice by intraperitoneal injection. Colonic inflammation, induced by addition of DSS (2.5%) to the drinking water, was measured as disease activity index (DAI) and histological score. Epithelial permeability and apoptosis were assessed by measuring FITC-dextran uptake and caspase-3 cleavage, respectively. Cecal bile acids were measured by HPLC-MS/MS. UDCA and LCA, but not 6-MUDCA, were protective against DSS-induced increases in epithelial permeability and colonic inflammation. Furthermore, UDCA and LCA inhibited colonic epithelial caspase-3 cleavage both in DSS-treated mice and in an in vitro model of cytokine-induced epithelial injury. HPLC-MS/MS analysis revealed UDCA administration to increase colonic LCA levels, whereas LCA administration did not alter UDCA levels. UDCA, and its primary metabolite, LCA, protect against intestinal inflammation in vivo, at least in part, by inhibition of epithelial apoptosis and promotion of barrier function. These data suggest that clinical trials of UDCA in IBD patients are warranted

Canine babesiosis and tick activity monitored using companion animal electronic health records in the UK

Recent publications highlighting autochthonous Babesia canis infection in dogs from Essex that have not travelled outside the UK are a powerful reminder of the potential for pathogen emergence in new populations. Here the authors use electronic health data collected from two diagnostic laboratories and a network of 392 veterinary premises to describe canine Babesia cases and levels of Babesia concern from January 2015 to March 2016, and the activity of ticks during December 2015–March 2016. In most areas of the UK, Babesia diagnosis in this population was rare and sporadic. In addition, there was a clear focus of Babesia cases in the affected area in Essex. Until February 2016, analysis of health records indicated only sporadic interest in Babesia largely in animals coming from overseas. Following media coverage in March 2016, there was a spike in owner concern that was geographically dispersed beyond the at-risk area. Tick activity (identified as ticks being removed from animals in veterinary consultations) was consistent but low during the period preceding the infections (<5 ticks/10,000 consultations), but increased in March. This highlights the use of electronic health data to describe rapidly evolving risk and concern that follows the emergence of a pathogen