Association of C3435T (rs1045642) polymorphism of the MDR1 gene with the increased risk of colorectal cancer in russian females from Central Russia

The objective of this study was to examine associations of single nucleotide polymorphisms, rs1045642 within the MDR1 gene and rs1799930 within the NAT2 gene, with the risk of colorectal cancer (CRC) in the population of Central Russi

Bioethics in Russia: a Complex Scientific Discipline at the Intersection of Philosophy, Biology, Culture and Law

The article describes the formation of bioethics — a complex scientific discipline — in our country. The emergence of bioethics in the world dates back to the 1970s of the 20th century, in the Soviet Union it began to take shape during the period of perestroika as part of the activities of Academician Ivan Timofeevich Frolov (1929–1999). Bioethics deals with ethical issues that arise during the introduction of the latest biomedical technologies into practice, the solution of which can have various medical, cultural, legal, and financial consequences. The latest biomedical technologies open up new possibilities for a person (heredity management, genetic passport, etc.) and put a person in a situation of choice. Their future depends on what choice people will make, and this choice is determined by the state of the culture of society. In the field of bioethics, regulation is crucial and should be comprehensive and multi-stage. Philosophers, culturologists, and ethicists formulate general principles of regulation based on the ideology of humanism. Further on, scientists consider these recommendations and develop appropriate codes of conduct, while lawyers consolidate the new understanding within the legal system. The formation of such an integrated approach in Russia took place within the framework of scientific structures created by Academician I. T. Frolov, — the AllUnion Interdepartmental Center for Human Sciences, the Institute of Man of the Russian Academy of Sciences, the Russian National Committee on Bioethics (RNKB), the Russian Committee on Bioethics under the Commission of the Russian Federation for UNESCO. In these structures, expert work was carried out on the main documents related to the interaction of the Russian Federation with the Council of Europe in the field of bioethics. RNKB prepared expert opinions for the Department of European Cooperation of the Ministry of Foreign Affairs of the Russian Federation and the Department for International Humanitarian Cooperation and Human Rights of the Ministry of Foreign Affairs of the Russian Federation regarding Russia's accession to international conventions on bioethics

Instructions for laboratory training in General Microbiology & Immunology for students of medical faculty

МИКРОБИОЛОГИЯАЛЛЕРГОЛОГИЯ И ИММУНОЛОГИЯЛАБОРАТОРНЫЕ РАБОТЫИНОСТРАННЫЕ СТУДЕНТЫПРАКТИЧЕСКИЕ НАВЫКИПРАКТИЧЕСКИЕ ЗАНЯТИЯИНСТРУКЦИИИММУНОЛОГИЯВ издании содержатся план, программа практических занятий и основные практические навыки по общей микробиологии и иммунологии

Geostatistical and geospatial assessment of soil pollution with heavy metals in Pavlograd city (Ukraine)

This paper presents the result of studies of the geostatistical and geospatial assessment heavy metal pollution in soils caused by various technogenic sources to assess the environmental impact of industrial agglomeration activitiy in the Pavlograd city. The main sources of technogenic pollution in the Pavlograd city and suburban areas are industrial enterprises, coal-fired plants, mine dumps and other. The vast number of analyzed soil samples with weakly acidic pH values from 6.3 to 6.8 is characterized as loam (63 %), the rest are sand (33 %) and clay soils (4 %). The correlation matrix of metals data indicates positive correlation with correlation coefficient r 2 > 0.5 among texture – Ni (0.705) and Pb – Zn (0.695) within the Pavlograd city area. In the case of Cu (2.73) and Cd (4.27), the geoaccumulation index indicated “moderately to strongly” and “strongly to extremely” polluted soils accordingly. Means of pollution index (PI) for heavy metals were between uncontaminated ( PI ≤ 1) and of moderate contaminated soils ( PI ≥ 1).The results of this study show that industrial enterprises’ activities lead to heavy metal contamination in the topsoil of the studied sites and close to the background values. Similar properties were confirmed by principal component analysis (PCA) and cluster analysis. The PCA and cluster analysis results indicate that Cd, Ni, Pb, Cu, Zn and Mn in topsoil were affected by the technogenic activity. The spatial distribution characteristics of Cu, Pb, Cd and Zn in the geochemical maps are also similar. Highlighted are some of the halos in Pavlograd of high density of total soil contamination with heavy metals. The metals come from anthropogenic sources, mainly produced with four industrial enterprises in Pavlograd city. Mostly they are concentrated in the Southeast and West of the city and suburban areas of the Pavlograd district. Therefore, it is necessary arrangements to predict of the development of ecologically dangerous state of environmental pollution within the area of industrial enterprises of city

IFIT1 differentially interferes with translation and replication of alphavirus genomes and promotes induction of type I interferon

Alphaviruses are a group of widely distributed human and animal pathogens. It is well established that their replication is sensitive to type I IFN treatment, but the mechanism of IFN inhibitory function remains poorly understood. Using a new experimental system, we demonstrate that in the presence of IFN-β, activation of interferon-stimulated genes (ISGs) does not interfere with either attachment of alphavirus virions to the cells, or their entry and nucleocapsid disassembly. However, it strongly affects translation of the virion-delivered virus-specific RNAs. One of the ISG products, IFIT1 protein, plays a major role in this translation block, although an IFIT1-independent mechanism is also involved. The 5'UTRs of the alphavirus genomes were found to differ significantly in their ability to drive translation in the presence of increased concentration of IFIT1. Prior studies have shown that adaptation of naturally circulating alphaviruses to replication in tissue culture results in accumulation of mutations in the 5'UTR, which increase the efficiency of the promoter located in the 5'end of the genome. Here, we show that these mutations also decrease resistance of viral RNA to IFIT1-induced translation inhibition. In the presence of higher levels of IFIT1, alphaviruses with wt 5'UTRs became potent inducers of type I IFN, suggesting a new mechanism of type I IFN induction. We applied this knowledge of IFIT1 interaction with alphaviruses to develop new attenuated variants of Venezuelan equine encephalitis and chikungunya viruses that are more sensitive to the antiviral effects of IFIT1, and thus could serve as novel vaccine candidates

The study of the functionality of cardiomyocytes obtained from induced pluripotent stem cells for the modeling of cardiac arrhythmias based on long QT syndrome

There are risk factors that lead the normal conduction of excitation in the heart into a chaotic one. These factors include hereditary and acquired channelopathies. Many dangerous changes in the work of the heart can be identified using the patient’s electrocardiogram. Such relatively easily detectable changes include the long QT interval syndrome (LQTS). Despite a relatively high prevalence of hereditary LQTS, to which it is necessary to add both hereditary and induced LQTS as well as the ease of detection on the ECG, the mechanism of reentry formation in this syndrome is still un­known. What should be noted is a high variability of the hereditary syndrome and the fact of the connection between the increase in the heart rate and the risk of cardiac arrest. After an electrophysiological study on individual cardiac cells from patients with the LQT syndrome, it became apparent that the search for a mechanism for the transition of the normal heart rhythm to chaotic and fibrillation cannot be limited to recording ion currents in single cells. To solve this problem, we need a model of the behavior of cardiac tissue which reflects the relationship of various factors and the risk of reentry. In order to create an experimental model of LQTS in our work, the iPSC of a pati­ent-specific line from a healthy patient was differentiated into a monolayer of cardiac cells and the parameters of the excitation propagation were studied depending on the stage of differentiation. It was shown that a stable value of the propagation velocity and the response to periodic stimulation in the range of physiological values, are reached after the 30th day of dif­ferentiation

Management factors and conditions of higher education students professional mobility formation

© 2016, Econjournals. All rights reserved. In contemporary terms of globalization and modernization of higher professional education the tasks of those specialists’ training are relevant, which not only can easily learn, but also adapt quickly to changing conditions and to the content of their professional activities. In this context, this article is aimed at the characteristics’ identifying of students’ professional mobility formation. The leading methods in the study of this problem are diagnostic, observational, proxy-metrical methods, study and generalization of the advanced pedagogical experience, methods of data statistical treatment using the package SPSS. The article reveals the students’ attitude to the phenomenon of professional mobility; the basic reasons that may lead to occupation or specialty change by the graduates after university graduation are studied; educational and management conditions are offered contributing to the efficiency of the process; dynamics of students’ professional mobility formation during their study at the university is defined. The contents of this article can be useful for faculty members of higher educational institutions in the selection and structuring of learning content and the implementation of career guidance with graduates of schools and colleges

The risk of developing autonomic dysreflexia during urodynamic testing in patients after spinal cord injury

Introduction. Autonomic dysreflexia (AD) is a life-threatening dangerous condition in patients with spinal cord injury (SCI) above the T6 segment level. It is characterized by a sudden rise in systolic blood pressure more than 20 mmHg, and unpredictable reactions from the autonomic nervous system. An episode of autonomic dysreflexia can lead to several cardiovascular catastrophes – heart attack and/or acute cerebrovascular accident up to a lethal outcome. Currently, there is no diagnostic algorithm and no way to determine risk factors for the occurrence of autonomic dysreflexia.Objective. To search for the most informative diagnostic criteria for autonomic dysreflexia in patients with spinal cord injury.Materials and methods. The study included 40 patients with SCI above the T6 segment. Depending on the SCI degree, two groups were distinguished. Group 1 (n = 14) included patients with complete spinal cord injury, advising category A on the ASIA scale. Group 2 (n = 26) included patients with incomplete spinal cord injury, classified as ASIA-B, C, D. For a preliminary assessment of the risk factors for the development of AD, an ADFSCI questionnaire was used, then a urodynamic study was conducted with simultaneous registration of systolic / diastolic blood pressure (SBP / DBP), and heart rate, which confirmed or denied the presence of AD in patients.Results. According to the ADFSCI questionnaire, most patients showed a high degree of severity of autonomic disorders, suggesting the presence of AD. Subsequently, this assumption was confirmed by the results of a urodynamic testing with simultaneous monitoring of SBP / DBP and heart rate. When comparing the groups, statistically significant differences were found in the parameters of the ADFSCI questionnaire, as well as in the indicators of SBP / DBP (at the points of maximum detrusor pressure and when the cystometric capacity is reached).Conclusion. The obtained results allow us to draw conclusions about the high incidence of AD in patients with SCI above the T6 segment and the need for a preliminary assessment of the risks of developing this condition based on the ADFSCI questionnaire before conducting a urodynamic study. Considering the possible complications of AD, the urodynamic testing should be accompanied by continuous monitoring of the indicators of the cardiovascular system