Towards a combined use of geophysics and remote sensing techniques for the characterization of a singular building: “El Torreón” (the tower) at Ulaca oppidum (Solosancho, Ávila, Spain)

This research focuses on the study of the ruins of a large building known as “El Torreón” (the Tower), belonging to the Ulaca oppidum (Solosancho, Province of Ávila, Spain). Different remote sensing and geophysical approaches have been used to fulfil this objective, providing a better understanding of the building’s functionality in this town, which belongs to the Late Iron Age (ca. 300–50 BCE). In this sense, the outer limits of the ruins have been identified using photogrammetry and convergent drone flights. An additional drone flight was conducted in the surrounding area to find additional data that could be used for more global interpretations. Magnetometry was used to analyze the underground bedrock structure and ground penetrating radar (GPR) was employed to evaluate the internal layout of the ruins. The combination of these digital methodologies (surface and underground) has provided a new perspective for the improved interpretation of “El Torreón” and its characteristics. Research of this type presents additional guidelines for better understanding of the role of this structure with regards to other buildings in the Ulaca oppidum. The results of these studies will additionally allow archaeologists to better plan future interventions while presenting new data that can be used for the interpretation of this archaeological complex on a larger scale

Metagenomics: DNA sequencing of environmental samples

While genomics has classically focused on pure, easy-to-obtain samples, such as microbes that grow readily in culture or large animals and plants, these organisms represent but a fraction of the living or once living organisms of interest. Many species are difficult to study in isolation, because they fail to grow in laboratory culture, depend on other organisms for critical processes, or have become extinct. DNA sequence-based methods circumvent these obstacles, as DNA can be directly isolated from live or dead cells in a variety of contexts, and have led to the emergence of a new field referred to as metagenomics

Phytoplasmas: genetics, diagnosis and relationships with the plant and insect host

Phytoplasmas cannot be cultivated in vitro, and remain the most poorly understood plant pathogens Despite this limitation, the investigation of their nature with the aid of modern tools has produced noteworthy results during the last 20 years. Using biochemical and molecular approaches, the phylogeny of the phytoplasmas has been described, their chromosomal and extrachromosomal components are being studied, and information on the localization, movement, and metabolic interference occurring in their insect and plant hosts accumulated. At the same time, the application of the new findings in phylogeny and genetics has aided the development of powerful diagnostic tools that have improved the ability to manage diseases which are induced by phytoplasma

PCR Mediated Whole genome amplification of phytoplasmas

A method was developed for genome analysis of phytoplasmas, bacterial plant pathogens that cannot be cultivated in vitro in cell-free media. The procedure includes a CsCl-bisbenzimide gradient buoyant centrifugation followed by polymerase chain reaction (PCR)-mediated whole genome amplification. The latter step involves digestion of the DNA by a restriction enzyme with an A/T-rich recognition sequence. Due to the different A/T content in the DNA of the pathogen and its plant host, the fragments originating from phytoplasma are shorter and are preferentially amplified in the PCR reaction. Products obtained were cloned and screened by dot-blot hybridization. Results showed that about 90% of recombinant clones appeared to harbor phytoplasma specific DNA inserts. Sequencing of randomly selected clones was carried out and comparison with the NCBI database confirmed the bacterial origin for the sequences, which have been assigned a putative function. The origin of the recombinant clones was further confirmed by the generation of specific amplicons from the phytoplasma-infected plant and not from the healthy control, using PCR primers devised from the sequences of the recombinant clones. This method could be used for genome-wide comparisons between phytoplasmas

Aplicación de la imagen multiespectral al estudio y conservación del arte rupestre postpaleolítico

Rock art deteriorates because of both environmental factors and visitor erosion which affect its conservation and, therefore, its study. Graphic recording carried out in the past is sometimes the only recorded evidence for what once existed. However, the graphic record also presents problems of preservation on the long-term, a problem particularly highlighted on modern color photographic materials. In this paper we deal with new approaches to solve this problem. We illustrate our work with the photographic collection about Levantine rock art, which belongs to the CSIC, and is held in the Department of Prehistory of Centro de Estudios Históricos. Two methods are highlighted: the use of the new Kodak Photo CD system, and the multiband Digital Imaging analysis.<br><br>El deterioro que padece el arte rupestre tanto por factores ambientales como antrópicos atañe a su conservación e investigación en el futuro. La documentación gráfica, en algunos casos el único vestigio disponible, se ve limitada también por problemas de conservación a largo plazo, en especial cuando se trata de fotografía en color. En este trabajo se plantean nuevas líneas de investigación para solventar estos problemas a partir de una investigación en curso en el Departamento de Prehistoria del Centro de Estudios Históricos (CSIC). Se trata de la conversión del importante archivo fotográfico del Corpus de Pintura Rupestre Levantina a formatos digitales (Kodak Photo CD) y la aplicación del tratamiento digital de imagen multiespectral a la investigación de estas manifestaciones artísticas

ECO4RUPA: 5G-IoT Inclusive and Intelligent Routing Ecosystem with Low-Cost Air Quality Monitoring

The increase and diversity of low-cost air quality (AQ) sensors, as well as their flexibility and low power consumption, offers us the opportunity to integrate them into broad AQ wireless sensor networks, with the aim of enabling real-time monitoring and higher spatial sampling density of pollution in all parts of cities. Considering that the vast majority of the population lives in cities and the increase in respiratory/allergic problems in a large part of the population, it is of great interest to offer services and applications to improve their quality of life by avoiding pollution exposure in their movements in the open air. In the ECO4RUPA project, we focus on this kind of service, proposing an inclusive and intelligent routing ecosystem carried out using a network of low-cost AQ sensors with the support of 5G communications along with official AQ monitoring stations, using spatial interpolation techniques to enhance its spatial resolution. The goal of this service is to calculate healthy walking and/or cycling routes according to the particular citizen’s profile and needs. We provide and analyse the results of the proposed route planner under different scenarios (different timetables, congestion road traffic, and routes) and different user profiles, with a special interest in citizens with asthma and pregnant women, since both have special needs. In summary, our approach can lead to an approximately average reduction in pollution exposure of 17.82% while experiencing an approximately average increase in distance travelled of 9.8%