50 research outputs found
A Model for the Development of the Rhizobial and Arbuscular Mycorrhizal Symbioses in Legumes and Its Use to Understand the Roles of Ethylene in the Establishment of these two Symbioses
We propose a model depicting the development of nodulation and arbuscular mycorrhizae. Both processes are dissected into many steps, using Pisum sativum L. nodulation mutants as a guideline. For nodulation, we distinguish two main developmental programs, one epidermal and one cortical. Whereas Nod factors alone affect the cortical program, bacteria are required to trigger the epidermal events. We propose that the two programs of the rhizobial symbiosis evolved separately and that, over time, they came to function together. The distinction between these two programs does not exist for arbuscular mycorrhizae development despite events occurring in both root tissues. Mutations that affect both symbioses are restricted to the epidermal program. We propose here sites of action and potential roles for ethylene during the formation of the two symbioses with a specific hypothesis for nodule organogenesis. Assuming the epidermis does not make ethylene, the microsymbionts probably first encounter a regulatory level of ethylene at the epidermisâoutermost cortical cell layer interface. Depending on the hormone concentrations there, infection will either progress or be blocked. In the former case, ethylene affects the cortex cytoskeleton, allowing reorganization that facilitates infection; in the latter case, ethylene acts on several enzymes that interfere with infection thread growth, causing it to abort. Throughout this review, the difficulty of generalizing the roles of ethylene is emphasized and numerous examples are given to demonstrate the diversity that exists in plants
Arbuscular mycorrhizal community structure on co-existing tropical legume trees in French Guiana
Aims
We aimed to characterise the arbuscular mycorrhizal fungal (AMF) community structure and potential edaphic determinants in the dominating, but poorly described, root-colonizing Paris-type AMF community on co-occurring Amazonian leguminous trees.
Methods
Three highly productive leguminous trees (Dicorynia guianensis, Eperua falcata and Tachigali melinonii were targeted) in species-rich forests on contrasting soil types at the Nouragues Research Station in central French Guiana. Abundant AMF SSU rRNA amplicons (NS31-AM1 & AML1-AML2 primers) from roots identified via trnL profiling were subjected to denaturing gradient gel electrophoresis (DGGE), clone library sequencing and phylogenetic analysis.
Results
Classical approaches targeting abundant SSU amplicons highlighted a diverse root-colonizing symbiotic AMF community dominated by members of the Glomeraceae. DGGE profiling indicated that, of the edaphic factors investigated, soil nitrogen was most important in influencing the AMF community and this was more important than any host tree species effect.
Conclusions
Dominating Paris-type mycorrhizal leguminous trees in Amazonian soils host diverse and novel taxa within the Glomeraceae that appear under edaphic selection in the investigated tropical forests. Linking symbiotic diversity of identified AMF taxa to ecological processes is the next challenge ahead
Une methode magnetique radiofrequence de localisation des defauts= dans les structures en composite carbone
Communication to : Congres COFREND 97, Nantes (France), 22-26 septembre 1997Available from INIST (FR), Document Supply Service, under shelf-number : 22419, issue : a.1997 n.157 / INIST-CNRS - Institut de l'Information Scientifique et TechniqueSIGLEFRFranc
Collaborative innovation and open innovation
Consumersâ demand for products has moved, since the eighties, toward products customized to personal needs. This change has incited firms to work closely with other organizations and especially customers, in order to anticipate better the future needs, inducing a growth in external relationships of firms. This trend is still at work in most of industries in which lead users impulse evolution in products. In consequence, the collaborative process continues to increase. At the same time, the efficiency of the production by in-house R&D of firmsâ decreases to such an extent that following Chesbrough (2003), many scholars advise firms to access knowledge externally and to develop innovation with an open process of development: the open innovation model. They incite firms to use all the external sources which are available to be the first to introduc ..