Dynein/dynactin regulate metaphase spindle length by targeting depolymerizing activities to spindle poles

During cell division metaphase spindles maintain constant length, whereas spindle microtubules continuously flux polewards, requiring addition of tubulin subunits at microtubule plus-ends, polewards translocation of the microtubule lattice, and removal of tubulin subunits from microtubule minus-ends near spindle poles. How these processes are coordinated is unknown. Here, we show that dynein/dynactin, a multi-subunit microtubule minus-end–directed motor complex, and NuMA, a microtubule cross-linker, regulate spindle length. Fluorescent speckle microscopy reveals that dynactin or NuMA inhibition suppresses microtubule disassembly at spindle poles without affecting polewards microtubule sliding. The observed uncoupling of these two components of flux indicates that microtubule depolymerization is not required for the microtubule transport associated with polewards flux. Inhibition of Kif2a, a KinI kinesin known to depolymerize microtubules in vitro, results in increased spindle microtubule length. We find that dynein/dynactin contribute to the targeting of Kif2a to spindle poles, suggesting a model in which dynein/dynactin regulate spindle length and coordinate flux by maintaining microtubule depolymerizing activities at spindle poles

Chandra X-ray Observatory Arcsecond Imaging of the Young, Oxygen-rich Supernova Remnant 1E0102.2-7219

We present observations of the young, Oxygen-rich supernova remnant 1E0102.2-7219 taken by the Chandra X-ray Observatory during Chandra's Orbital Activation and Checkout phase. The boundary of the blast wave shock is clearly seen for the first time, allowing the diameter of the remnant and the mean blast wave velocity to be determined accurately. The prominent X-ray bright ring of material may be the result of the reverse shock encountering ejecta; the radial variation of O VII vs. O VIII emission indicates an ionizing shock propagating inwards, possibly through a strong density gradient in the ejecta. We compare the X-ray emission to Australia Telescope Compact Array 6 cm radio observations (Amy and Ball) and to archival Hubble Space Telescope [O III] observations. The ring of radio emission is predominantly inward of the outer blast wave, consistent with an interpretation as synchrotron radiation originating behind the blast wave, but outward of the bright X-ray ring of emission. Many (but not all) of the prominent optical filaments are seen to correspond to X-ray bright regions. We obtain an upper limit of ~9e33 erg/s (3 sigma) on any potential pulsar X-ray emission from the central region.Comment: Accepted for pulication in Ap. J. Letters. 4 pages, 6 figures (one color figure). Formatted with emulateapj5. Revised to incorporate copyediting changes. High-resolution postscript (3.02MB) and tiff versions of the color figure are available from http://chandra.harvard.edu/photo/cycle1/0015multi/index.htm

Magnetic Field Effects on the Head Structure of Protostellar Jets

We present the results of 3-D SPMHD numerical simulations of supermagnetosonic, overdense, radiatively cooling jets. Two initial magnetic configurations are considered: (i) a helical and (ii) a longitudinal field. We find that magnetic fields have important effects on the dynamics and structure of radiative cooling jets, especially at the head. The presence of a helical field suppresses the formation of the clumpy structure which is found to develop at the head of purely hydrodynamical jets. On the other hand, a cooling jet embedded in a longitudinal magnetic field retains clumpy morphology at its head. This fragmented structure resembles the knotty pattern commonly observed in HH objects behind the bow shocks of HH jets. This suggests that a strong (equipartition) helical magnetic field configuration is ruled out at the jet head. Therefore, if strong magnetic fields are present, they are probably predominantly longitudinal in those regions. In both magnetic configurations, we find that the confining pressure of the cocoon is able to excite short-wavelength MHD K-H pinch modes that drive low-amplitude internal shocks along the beam. These shocks are not strong however, and it likely that they could only play a secondary role in the formation of the bright knots observed in HH jets.Comment: 14 pages, 2 Gif figures, uses aasms4.sty. Also available on the web page http://www.iagusp.usp.br/preprints/preprint.html. To appear in The Astrophysical Journal Letter

M 33 X-7: ChASeM33 reveals the first eclipsing black hole X-ray binary

The first observations conducted as part of the Chandra ACIS survey of M 33 (ChASeM33) sampled the eclipsing X-ray binary M 33 X-7 over a large part of the 3.45 d orbital period and have resolved eclipse ingress and egress for the first time. The occurrence of the X-ray eclipse allows us to determine an improved ephemeris of mid-eclipse and binary period as HJD (2453639.119+-0.005) +- N x (3.453014+-0.000020) and constrain the eclipse half angle to (26.5+-1.1) degree. There are indications for a shortening of the orbital period. The X-ray spectrum is best described by a disk blackbody spectrum typical for black hole X-ray binaries in the Galaxy. We find a flat power density spectrum and no significant regular pulsations were found in the frequency range of 10^{-4} to 0.15 Hz. HST WFPC2 images resolve the optical counterpart, which can be identified as an O6III star with the help of extinction and colour corrections derived from the X-ray absorption. Based on the optical light curve, the mass of the compact object in the system most likely exceeds 9 M_sun. This mass, the shape of the X-ray spectrum and the short term X-ray time variability identify M 33 X-7 as the first eclipsing black hole high mass X-ray binary.Comment: 14 pages, 5 figures, ApJ accepte

An X-ray upper limit on the presence of a Neutron Star for the Small Magellanic Cloud and Supernova Remnant 1E0102.2-7219

We present Chandra X-ray Observatory archival observations of the supernova remnant 1E0102.2-7219, a young Oxygen-rich remnant in the Small Magellanic Cloud. Combining 28 ObsIDs for 324 ks of total exposure time, we present an ACIS image with an unprecedented signal-to-noise ratio (mean S/N ~ sqrt(S) ~6; maximum S/N > 35) . We search within the remnant, using the source detection software {\sc wavdetect}, for point sources which may indicate a compact object. Despite finding numerous detections of high significance in both broad and narrow band images of the remnant, we are unable to satisfactorily distinguish whether these detections correspond to emission from a compact object. We also present upper limits to the luminosity of an obscured compact stellar object which were derived from an analysis of spectra extracted from the high signal-to-noise image. We are able to further constrain the characteristics of a potential neutron star for this remnant with the results of the analysis presented here, though we cannot confirm the existence of such an object for this remnant.Comment: Accepted to the Astrophysical Journa

High-resolution x-ray telescopes

High-energy astrophysics is a relatively young scientific field, made possible by space-borne telescopes. During the half-century history of x-ray astronomy, the sensitivity of focusing x-ray telescopes-through finer angular resolution and increased effective area-has improved by a factor of a 100 million. This technological advance has enabled numerous exciting discoveries and increasingly detailed study of the high-energy universe-including accreting (stellar-mass and super-massive) black holes, accreting and isolated neutron stars, pulsar-wind nebulae, shocked plasma in supernova remnants, and hot thermal plasma in clusters of galaxies. As the largest structures in the universe, galaxy clusters constitute a unique laboratory for measuring the gravitational effects of dark matter and of dark energy. Here, we review the history of high-resolution x-ray telescopes and highlight some of the scientific results enabled by these telescopes. Next, we describe the planned next-generation x-ray-astronomy facility-the International X-ray Observatory (IXO). We conclude with an overview of a concept for the next next-generation facility-Generation X. The scientific objectives of such a mission will require very large areas (about 10000 m2) of highly-nested lightweight grazing-incidence mirrors with exceptional (about 0.1-arcsecond) angular resolution. Achieving this angular resolution with lightweight mirrors will likely require on-orbit adjustment of alignment and figure.Comment: 19 pages, 11 figures, SPIE Conference 7803 "Adaptive X-ray Optics", part of SPIE Optics+Photonics 2010, San Diego CA, 2010 August 2-