36 research outputs found
Complex symplectic lie algebras with large abelian subalgebras
We present two constructions of complex symplectic structures on Lie algebras with large Abelian ideals. In particular, we completely classify complex symplectic structures on almost Abelian Lie algebras. By considering compact quotients of their corresponding connected, simply connected Lie groups we obtain many examples of complex symplectic manifolds which do not carry (hyper)kähler metrics. We also produce examples of compact complex symplectic manifolds endowed with a fibration whose fibers are Lagrangian tori
Closed forms and multi-moment maps
We extend the notion of multi-moment map to geometries defined by closed
forms of arbitrary degree. We give fundamental existence and uniqueness results
and discuss a number of essential examples, including geometries related to
special holonomy. For forms of degree four, multi-moment maps are guaranteed to
exist and are unique when the symmetry group is (3,4)-trivial, meaning that the
group is connected and the third and fourth Lie algebra Betti numbers vanish.
We give a structural description of some classes of (3,4)-trivial algebras and
provide a number of examples.Comment: 36 page
Isochronal annealing effects on local structure, crystalline fraction, and undamaged region size of radiation damage in Ga-stabilized -Pu
The effects on the local structure due to self-irradiation damage of Ga
stabilized -Pu stored at cryogenic temperatures have been examined
using extended x-ray absorption fine structure (EXAFS) experiments. Extensive
damage, seen as a loss of local order, was evident after 72 days of storage
below 15 K. The effect was observed from both the Pu and Ga sites, although
less pronounced around Ga. Isochronal annealing was performed on this sample to
study the annealing processes that occur between cryogenic and room temperature
storage conditions, where damage is mostly reversed. Damage fractions at
various points along the annealing curve have been determined using an
amplitude-ratio method, standard EXAFS fitting, and a spherical crystallite
model, and provide information complementary to previous electrical
resistivity- and susceptibility-based isochronal annealing studies. The use of
a spherical crystallite model accounts for the changes in EXAFS spectra using
just two parameters, namely, the crystalline fraction and the particle radius.
Together, these results are discussed in terms of changes to the local
structure around Ga and Pu throughout the annealing process and highlight the
unusual role of Ga in the behavior of the lowest temperature anneals.Comment: 13 pages, 10 figure
Complex symplectic structures on Lie algebras
We investigate Lie algebras endowed with a complex symplectic structure and develop a method, called complex symplectic oxidation, to construct certain complex symplectic Lie algebras of dimension 4n+4 from those of dimension 4n. We specialize this construction to the nilpotent case and apply complex symplectic oxidation to classify eight-dimensional nilpotent complex symplectic Lie algebras
The mitochondrial Hsp70 chaperone Ssq1 facilitates Fe/S cluster transfer from Isu1 to Grx5 by complex formation
Systematic identification of metabolites controlling gene expression in E. coli
Metabolism controls gene expression through allosteric interactions between metabolites and transcription factors. These interactions are usually measured with in vitro assays, but there are no methods to identify them at a genome-scale in vivo. Here we show that dynamic transcriptome and metabolome data identify metabolites that control transcription factors in E. coli. By switching an E. coli culture between starvation and growth, we induce strong metabolite concentration changes and gene expression changes. Using Network Component Analysis we calculate the activities of 209 transcriptional regulators and correlate them with metabolites. This approach captures, for instance, the in vivo kinetics of CRP regulation by cyclic-AMP. By testing correlations between all pairs of transcription factors and metabolites, we predict putative effectors of 71 transcription factors, and validate five interactions in vitro. These results show that combining transcriptomics and metabolomics generates hypotheses about metabolism-transcription interactions that drive transitions between physiological states
Molecular architecture and electron transfer pathway of the Stn family transhydrogenase
Abstract The challenge of endergonic reduction of NADP+ using NADH is overcome by ferredoxin-dependent transhydrogenases that employ electron bifurcation for electron carrier adjustments in the ancient Wood-Ljungdahl pathway. Recently, an electron-bifurcating transhydrogenase with subunit compositions distinct from the well-characterized Nfn-type transhydrogenase was described: the Stn complex. Here, we present the single-particle cryo-EM structure of the Stn family transhydrogenase from the acetogenic bacterium Sporomusa ovata and functionally dissect its electron transfer pathway. Stn forms a tetramer consisting of functional heterotrimeric StnABC complexes. Our findings demonstrate that the StnAB subunits assume the structural and functional role of a bifurcating module, homologous to the HydBC core of the electron-bifurcating HydABC complex. Moreover, StnC contains a NuoG-like domain and a GltD-like NADPH binding domain that resembles the NfnB subunit of the NfnAB complex. However, in contrast to NfnB, StnC lost the ability to bifurcate electrons. Structural comparison allows us to describe how the same fold on one hand evolved bifurcation activity on its own while on the other hand combined with an associated bifurcating module, exemplifying modular evolution in anaerobic metabolism to produce activities critical for survival at the thermodynamic limit of life
Cell Reports
The stringent response enables metabolic adaptation of bacteria under stress conditions and is governed by RelA/SpoT Homolog (RSH)-type enzymes. Long RSH-type enzymes encompass an N-terminal domain (NTD) harboring the second messenger nucleotide (p)ppGpp hydrolase and synthetase activity and a stress-perceiving and regulatory C-terminal domain (CTD). CTD-mediated binding of Rel to stalled ribosomes boosts (p)ppGpp synthesis. However, how the opposing activities of the NTD are controlled in the absence of stress was poorly understood. Here, we demonstrate on the RSH-type protein Rel that the critical regulative elements reside within the TGS (ThrRS, GTPase, and SpoT) subdomain of the CTD, which associates to and represses the synthetase to concomitantly allow for activation of the hydrolase. Furthermore, we show that Rel forms homodimers, which appear to control the interaction with deacylated-tRNA, but not the enzymatic activity of Rel. Collectively, our study provides a detailed molecular view into the mechanism of stringent response repression in the absence of stress