High night temperatures during grain number determination reduce wheat and barley grain yield: a field study

Warm nights are a widespread predicted feature of climate change. This study investigated the impact of high nighttemperatures during the critical period for grain yield determination in wheat and barley crops under field conditions,assessing the effects on development, growth and partitioning crop-level processes driving grain number perunit area (GN). Experiments combined: (i) two contrasting radiation and temperature environments: late sowing in2011 and early sowing in 2013, (ii) two well-adapted crops with similar phenology: bread wheat and two-row maltingbarley and (iii) two temperature regimes: ambient and high night temperatures. The night temperature increase (ca.3.9 °C in both crops and growing seasons) was achieved using purpose-built heating chambers placed on the crop at19:000 hours and removed at 7:00 hours every day from the third detectable stem node to 10 days post-flowering.Across growing seasons and crops, the average minimum temperature during the critical period ranged from 11.2 to17.2 °C. Wheat and barley grain yield were similarly reduced under warm nights (ca. 7% °C1), due to GN reductions(ca. 6% °C1) linked to a lower number of spikes per m2. An accelerated development under high night temperaturesled to a shorter critical period duration, reducing solar radiation capture with negative consequences forbiomass production, GN and therefore, grain yield. The information generated could be used as a starting point todesign management and/or breeding strategies to improve crop adaptation facing climate change.Fil: García, Guillermo Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal. Cátedra de Cerealicultura; ArgentinaFil: Dreccer, M. Fernanda. University of Queensland. Cooper Laboratory. CSIRO Plant Industry; AustraliaFil: Miralles, Daniel Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal. Cátedra de Cerealicultura; ArgentinaFil: Serrago, Roman Augusto. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal. Cátedra de Cerealicultura; Argentin

Yielding to the image: how phenotyping reproductive growth can assist crop improvement and production

Reproductive organs are the main reason we grow and harvest most plant species as crops, yet they receive less attention from phenotyping due to their complexity and inaccessibility for analysis. This review highlights recent progress towards the quantitative high-throughput phenotyping of reproductive development, focusing on three impactful areas that are pivotal for plant breeding and crop production. First, we look at phenotyping phenology, summarizing the indirect and direct approaches that are available. This is essential for analysis of genotype by environment, and to enable effective management interpretation and agronomy and physiological interventions. Second, we look at pollen development and production, in addition to anther characteristics, these are critical points of vulnerability for yield loss when stress occurs before and during flowering, and are of particular interest for hybrid technology development. Third, we elaborate on phenotyping yield components, indirectly or directly during the season, with a numerical or growth related approach and post-harvest processing. Finally, we summarise the opportunities and challenges ahead for phenotyping reproductive growth and their feasibility and impact, with emphasis on plant breeding applications and targeted yield increases

Multi-donor × elite-based populations reveal QTL for low-lodging wheat

Low-lodging high-yielding wheat germplasm and SNP-tagged novel alleles for lodging were identified in a process that involved selecting donors through functional phenotyping for underlying traits with a designed phenotypic screen, and a crossing strategy involving multiple-donor × elite populations

Meeting the challenges facing wheat production: The strategic research agenda of the Global Wheat Initiative

Wheat occupies a special role in global food security since, in addition to providing 20% of our carbohydrates and protein, almost 25% of the global production is traded internationally. The importance of wheat for food security was recognised by the Chief Agricultural Scientists of the G20 group of countries when they endorsed the establishment of the Wheat Initiative in 2011. The Wheat Initiative was tasked with supporting the wheat research community by facilitating collaboration, information and resource sharing and helping to build the capacity to address challenges facing production in an increasingly variable environment. Many countries invest in wheat research. Innovations in wheat breeding and agronomy have delivered enormous gains over the past few decades, with the average global yield increasing from just over 1 tonne per hectare in the early 1960s to around 3.5 tonnes in the past decade. These gains are threatened by climate change, the rapidly rising financial and environmental costs of fertilizer, and pesticides, combined with declines in water availability for irrigation in many regions. The international wheat research community has worked to identify major opportunities to help ensure that global wheat production can meet demand. The outcomes of these discussions are presented in this paper

Adaptation of wheat, barley, canola, field pea and chickpea to the thermal environments of Australia

Warming trends involve two agronomically relevant aspects: a gradual increase in long-term mean temperature with the primary effect of shifting phenological patterns, and an increasing incidence of heat waves. Depending on timing, intensity and duration, heat can reduce crop growth and disrupt reproduction. Agronomic and breeding adaptations to elevated temperature have been listed but there is an overall lack of frameworks for systematic analysis. This paper provides agronomic and physiological background for the quantitative assessment of spatial patterns of the thermal regimes for wheat, barley, canola, field pea and chickpea. First, we revise the notion that Australian agriculture is ‘European’ and ill-adapted to the local environments. By showing that Australian agriculture in the southern and western regions is rather Levantine, we advance a more accurate and relevant framework to the thermal regimes of winter crops. Second, we outline the direct and indirect effects of temperature on crop traits and highlight the limitations of different approaches to investigate crop responses to temperature. This is important to make explicit the assumptions of studies dealing with crop responses to temperature; for example, indirect effects of temperature on crops mediated by effects on weeds, pathogens or herbivores could be important. Third, we compare the cardinal temperatures (including base, optimal, and critical thresholds) of our target crops. Cardinal temperatures respond to both natural and agronomic selection and are relevant for crop adaptation. Fourth, we develop a conceptual framework to assess thermal effects on crop yield and adaptive practices and traits, based on the notions of yield being a primary function of seed number, the species-specific critical window for the determination of seed number, and two complementary perspectives involving the photothermal quotient and crop growth rate in the critical window. The framework accounts for both aspects of warming: non-stressful elevated temperature and heat stress. Testable propositions are advanced that inform future research on crop adaptation to elevated temperature

Quantitative dynamics of stem water soluble carbohydrates in wheat can be monitored in the field using hyperspectral reflectance

The capacity of wheat to store water soluble carbohydrates (WSC) in the stem is regarded as a promising trait to buffer yield in environments with limited water availability. A high throughput, field-applicable, phenotyping technique would not only benefit agronomy/physiology applications but also help its quantification in wheat breeding programmes. The aim of this study was to evaluate if it was possible to estimate the concentration (WSCc, mgg-1) and amount (WSCa, gm-2) of stem WSC non-destructively and in situ using hyperspectral data obtained in wheat canopies, as opposed to currently available labour intensive laboratory methods. Hyperspectral reflectance data were obtained proximally at varying developmental stages from the canopy of wheat trials with a limited number of related genotypes growing under a range of management treatments, in two successive years. Data were calibrated, firstly independently for each year and then jointly, to provide a measure of stem WSC using partial least squares regression on wavelengths in the range of 350-1290nm. Pre-treated spectra (second derivative) enabled calibrations for the combined years with concentration (WSCc, mgg-1) (r2=0.90) and amount (WSCa, gm-2) (r2=0.88) of water soluble carbohydrate in the stems. In addition, from the same measurement, other canopy properties, leaf area index and canopy water content, could be simultaneously predicted. This study has shown that calibration models from canopy level data can robustly predict the dynamics of stem WSC throughout crop stages and treatments, while at the same time including variation in indices diagnostic of crop water and cover status, such as the Water Index and Enhanced Vegetation Index. Promising WSC prediction using spectral data below 1000nm needs to be investigated further, in order to harness the potential for impact using low cost silicon detectors

Pot size matters revisited: Does container size affect the response to elevated CO2 and our ability to detect genotypic variability in this response in wheat?

Many studies have investigated the effect of elevated CO2 (eCO2) in wheat, although few have evaluated the potential of genotypic variability in the response. Such studies are the next logical step in wheat climate change adaptation research, and they will require the evaluation of large numbers of genotypes. For practical reasons the preliminary studies are most likely to be conducted in controlled environments. There have been concerns that the root restriction related to container-grown plants can influence (1) the response to eCO2, (2) the detection of genotypic variability for various traits of interest, and (3) the ability to find the genotypes most responsive to eCO2. In the present study we evaluated two sizes of container - 1.4L pots and 7.5L columns - side-by side in a glasshouse environment and found that for 14 of 23 traits observed environment effects (ambient CO2, eCO2 or eCO2 and high temperature) were not consistent between plants grown in pots and in columns. More importantly, of the 21 traits showing genotypic variability, only 8 showed consistent genotype differences and rankings across both container types. Statistical analyses conducted separately for plants grown in pots or in columns showed different cultivars as being the most responsive to elevated CO2 and would thus, have led to different conclusions. This study is intended as a message of caution to controlled environment experimenters: using small containers can artificially create conditions that could either hide or overly express genotypic variability in some traits in response to eCO2 compared with what might be expected in larger containers

Vernalisation and photoperiod sensitivity in wheat: Impact on canopy development and yield components

Genetic variation in the VERNALIZATION1 (VRN1) and PHOTOPERIOD1 (PPD1) genes, which control the vernalisation and photoperiod response, underpin wheat adaptation to different environments. Near isogenic lines were used to investigate the role of allelic combinations of VRN1 and PPD-D1, including new alleles for VRN1-A1, on the length of developmental phases, dynamics of leaf and tiller appearance and yield components in complementary irrigated field trials relevant to low latitude wheat growing areas and controlled conditions. Allelic differences in VRN1 had a stronger effect on the duration of the vegetative phase, while photoperiod sensitivity at PPD-D1 lengthened the stem elongation phase (SE) by up to 23%. If a phase was lengthened, flowering was delayed. The level of response to daylength during stem elongation (SE) introduced by photoperiod sensitive alleles was dependent on the VRN1 composition and vernalisation status. A longer SE under short days was achieved by PPD1 sensitive genotypes when one VRN1 spring allele was present and plants were vernalised. The duration of SE was weakly related to spike dry weight m−2 at DC65 in the field but did not translate into higher grain number m−2. In the field, lines with two to three VRN1 spring alleles had shortest development phases, including SE, close flowering dates, sampled similar temperature environments at different stages, and achieved high yields. Yield advantage was explained by higher biomass, harvest index, grain number m−2 and thousand kernel weight. Genotypes with three winter VRN1 alleles were comparatively disadvantaged, with a longer vegetative phase placing SE under higher temperatures. Allelic differences in both genes caused large variation in leaf and tiller number generation but also in tiller mortality and individual leaf size, lessening the impact on leaf area. Changes in plant morphology and yield components that did not seem mediated via the influence of development genes on the duration of different stages and their impact on resource capture deserve further investigation

Genotypic variability in the response to elevated CO2 of wheat lines differing in adaptive traits

Atmospheric CO2 levels have increased from ∼280ppm in the pre-industrial era to 391ppm in 2012. High CO2 concentrations stimulate photosynthesis in C3 plants such as wheat, but large variations have been reported in the literature in the response of yield and other traits to elevated CO2 (eCO2). Few studies have investigated genotypic variation within a species to address issues related to breeding for specific adaptation to eCO2. The objective of this study was to determine the response to eCO2 of 20 wheat lines which were chosen for their contrasting expression in tillering propensity, water soluble carbohydrate (WSC) accumulation in the stem, early vigour and transpiration efficiency. Experiments were performed in control environment chambers and in a glasshouse with CO2 levels controlled at either 420ppm (local ambient) or 700ppm (elevated). The results showed no indication of a differential response to eCO2 for any of these lines and adaptive traits were expressed in a consistent manner in ambient and elevated CO2 environments. This implies that for these traits, breeders could expect consistent rankings in the future, assuming these results are validated under field conditions. Additional climate change impacts related to drought and high temperature are also expected to interact with these traits such that genotype rankings may differ from the unstressed condition