Oxidative stress induced by the Fe2+/ascorbic acid system or model ischemia in vitro: effect of carvedilol and pyridoindole antioxidant SMe1EC2 in young and adult rat brain tissue

New effective strategies and new highly effective neuroprotective agents are being searched for the therapy of human stroke and cerebral ischemia. The compound SMe1EC2 is a new derivative of stobadine, with enhanced antioxidant properties compared to the maternal drug. Carvedilol, a non-selective beta-blocker, possesses besides its cardioprotective and vasculoprotective properties also an antioxidant effect. We compared the effect of carvedilol and SMe1EC2, antioxidants with a similar chemical structure, in two experimental models of oxidative stress in young and adult rat brain tissue. SMe1EC2 was found to improve the resistance of hippocampal neurons to ischemia in vitro in young and even in 18-month-old rats and inhibited formation of protein carbonyl groups induced by the Fe2+/ascorbic acid pro-oxidative system in brain cortex homogenates of young rats. Carvedilol exerted a protective effect only in the hippocampus of 2-month-old rats and that at the concentration 10-times higher than did SMe1EC2. The inhibitory effect of carvedilol on protein carbonyl formation induced by the pro-oxidative system was not proved in the cortex of either young or adult rats. An increased baseline level of the content of protein carbonyl groups in the adult versus young rat brain cortex confirmed age-related changes in neuronal tissue and may be due to increased production of reactive oxygen species and low antioxidant defense mechanisms in the adult rat brain. The results revealed the new pyridoindole SMe1EC2 to be more effective than carvedilol in neuroprotection of rat brain tissue in both experimental models involving oxidative stress

COVID-19 infection in adult patients with hematological malignancies: a European Hematology Association Survey (EPICOVIDEHA)

Background: Patients with hematological malignancies (HM) are at high risk of mortality from SARS-CoV-2 disease 2019 (COVID-19). A better understanding of risk factors for adverse outcomes may improve clinical management in these patients. We therefore studied baseline characteristics of HM patients developing COVID-19 and analyzed predictors of mortality. Methods: The survey was supported by the Scientific Working Group Infection in Hematology of the European Hematology Association (EHA). Eligible for the analysis were adult patients with HM and laboratory-confirmed COVID-19 observed between March and December 2020. Results: The study sample includes 3801 cases, represented by lymphoproliferative (mainly non-Hodgkin lymphoma n = 1084, myeloma n = 684 and chronic lymphoid leukemia n = 474) and myeloproliferative malignancies (mainly acute myeloid leukemia n = 497 and myelodysplastic syndromes n = 279). Severe/critical COVID-19 was observed in 63.8% of patients (n = 2425). Overall, 2778 (73.1%) of the patients were hospitalized, 689 (18.1%) of whom were admitted to intensive care units (ICUs). Overall, 1185 patients (31.2%) died. The primary cause of death was COVID-19 in 688 patients (58.1%), HM in 173 patients (14.6%), and a combination of both COVID-19 and progressing HM in 155 patients (13.1%). Highest mortality was observed in acute myeloid leukemia (199/497, 40%) and myelodysplastic syndromes (118/279, 42.3%). The mortality rate significantly decreased between the first COVID-19 wave (March–May 2020) and the second wave (October–December 2020) (581/1427, 40.7% vs. 439/1773, 24.8%, p value < 0.0001). In the multivariable analysis, age, active malignancy, chronic cardiac disease, liver disease, renal impairment, smoking history, and ICU stay correlated with mortality. Acute myeloid leukemia was a higher mortality risk than lymphoproliferative diseases. Conclusions: This survey confirms that COVID-19 patients with HM are at high risk of lethal complications. However, improved COVID-19 prevention has reduced mortality despite an increase in the number of reported cases.EPICOVIDEHA has received funds from Optics COMMITTM (COVID-19 Unmet Medical Needs and Associated Research Extension) COVID-19 RFP program by GILEAD Science, United States (Project 2020-8223)

Energy absorbing 4D printed meta-sandwich structures: load cycles and shape recovery

The present study investigates the behavior of solid cellular structures in polylactic acid (PLA) achieved by FDM technology (fusion deposition modelling). The geometries are permanently deformed by compressive stress and then subjected to shape recovery through the application of a thermal stimulus. The structures are submitted to medium-high and medium-low compression stresses, evaluating the mechanical properties and the absorption energy as the number of cycles varies. The study shows that the ability to absorb energy is related to the density of the model, as well as the degree of damage observed, which increases with increasing number of load cycles. The strongest geometry is the lozenge grid, which is the most reliable. It shows no damage with increasing compression cycles and keeps its capability to absorb energy almost constant. The increase in lozenge grid density leads to an improvement in both mechanical strength and absorption energy, as well as a lower incidence of microcracks in the geometry itself due to the repeated load cycles. These results open up a broad spectrum of applications of custom-designed solid cellular structures in the field of energy absorption and damping

Life Cycle Assessment (LCA) and Multi Criteria Decision Analysis (MCDA) of eco-friendly packaging for dairy products and fourth range

In the current European regulatory environment, there is a growing emphasis on the need to develop fourth range and dairy packaging materials. which are more environmentally sustainable. In response to this need, a Life Cycle Analysis (LCA) was conducted to assess the suitability of replacing traditional polystyrene foam boxes and polypropylene (PP) and polyethylene terephthalate (PET) trays with packaging made of corrugated cardboard and coated with bioplastic. Life cycle analysis shows that bioplastic coated cardboard packaging has a lower environmental impact, despite the impact of corn and sugar cane bioplastic production. In the case of dairy packaging, an improved disposal scenario or scenario has been considered, in particular in terms of recycling. In the second case, raw materials that are totally or partially recyclable were considered. For the cardboard tray, recyclability is 100%, while for PET and PP respectively 50% and 4%, due to insufficient recycling and decontamination processes. In this scenario, the impact of fossil plastics decreases substantially, although corrugated and bioplastic packaging is again the best choice. To confirm these results, a multi-criteria decision analysis (multi-criteria Decision Analysis) was conducted, which corroborated the conclusions obtained through life cycle analysis

Life Cycle Assessment (LCA) of ceramic sanitaryware: focus on the production process and analysis of scenario

The reduction of environmental impact is today the main challenge of the ceramic industry that is always more focusing on materials in line with the principles of economic and environmental sustainability. In this context, this study addresses the implementation of a Life Cycle Assessment (LCA) on the production of ceramic sanitaryware, based on a cradle-to-grave analysis. Specifically, the process was considered from raw materials until the product is manufactured, excluding the disposal phase except for process waste. The analysis of the impact assessment considers three different scenarios: (i) The first examines the current state; (ii) the second considers the recovery of fired waste and water as well as the replacement of firing and annealing ovens with new generation ovens; (iii) the third, in addition to the technologies used in the second, proposes the use of a photovoltaic system to produce green energy and, additionally, a "plant" energy recovery system. The results show how production processes have a considerable impact on the environment, in terms of energy consumption and materials. Moreover, the use of a photovoltaic system together with the recovery of water allows a significant reduction of environmental impacts. In contrast, the crushing processes for the recovery of fired waste worsen the environmental performance of the plant, because of the high consumption of electricity. Therefore, by improving the waste recovery system and adopting the solutions of the third scenario in terms of energy savings, it would be possible to reduce the environmental burden of the production system considerably. At the same time, the use of additional equipment and production processes increases the costs of the manufacturing and has a significant impact on maintenance

Life cycle assessment (LCA) of PET and PLA bottles for the packaging of fresh pasteurised milk. The role of the manufacturing process and the disposal scenario

Environmental protection issues are at the forefront of the vast majority of the media and public opinion. The study in question revealed the life cycle of a product commonly used in the home, the bottle of fresh pasteurised milk. The materials used for the manufacturing of the bottles are PET (PolyEthylene Terephthalate), plastic derived from fossil resources, and PLA (PolyLactic Acid), bioplastic derived from sugar cane, therefore from renewable resources. The life cycle of the bottles was carried out by highlighting the extraction phase of raw materials, the production of polymer, bottle, other packaging and distribution of milk and lastly, final disposal, excluding the phase of use, not significant for the purposes of this study. The studies were focused on two disposal scenarios, the current scenario and the one that is likely to take place in 10 years. In the current disposal scenario, there is no clear reduction in environmental impact from the comparison of the materials examined. With reference to the 2030 disposal scenario, the PLA turns out to be much more environmentally friendly

On the Mechanism of Levosimendan-Induced Dopamine Release in the Striatum of Freely Moving Rats

The Ca2+ sensitizer levosimendan (LEV) improves myocardial contractility by enhancing the sensitivity of the contractile apparatus to Ca2+. In addition, LEV promotes Ca2+ entry through L-type channels in human cardiac myocytes. In this study, which was performed using microdialysis, infusion of LEV at 0.25 μM for 160 min increased dopamine (DA) concentrations (up to fivefold baseline) in dialysates from the striatum of freely moving rats. Ca2+ omission from the perfusion fluid abolished baseline DA release and greatly decreased LEV-induced DA release. Reintroduction of Ca2+ in the perfusion fluid restored LEV-induced DA release. Chelation of intracellular Ca2+ by co-infusing 1,2-bis (o-amino-phenoxy)ethane-N,N,N′,N′-tetraacetic acid tetra (acetoxymethyl) ester (BAPTA-AM, 0.2 mM) did not affect basal DA release and scarcely affected LEV-induced increases in dialysate DA. In addition, co-infusion of the L-type (Cav 1.1 – 1.3) voltage-sensitive Ca2+-channel inhibitor nifedipine failed to inhibit LEV-induced increases in dialysate DA, which, in contrast, was inhibited by co-infusion of the N-type (Cav 2.2) voltage-sensitive Ca2+-channel inhibitor ω-conotoxin GVIA. We conclude that LEV promotes striatal extracellular Ca2+ entry through N-type Ca2+ channels with a consequent increase in DA release. Keywords:: levosimendan, microdialysis, striatal dopamine, calcium entr