Production of carbonized micro-patterns by photolithography and pyrolysis

The preparation of carbon micro-patterns is reported in this paper. Different carbon micro-patterns were created using photolithography of the epoxy-based negative photoresist SU-8. Photoresist patterns were optimized in terms of resolution and aspect ratio and subsequently subjected to pyrolysis to obtain carbonized and conductive 3D structures. The latter step requires the optimization of the resist cross-linking time as well as the temperature and time of the resist post-bake. This step is crucial in order to avoid any severe modification of the geometry of the patterns produced during the actual pyrolysis. By observing optical and scanning electron microscope images, the morphology of the structures before and after pyrolysis was studied and the same patterns were also characterized by a laser probe profilometer. Finally, the thus obtained carbon patterns on Si wafers were used to carry out cell culture tests with Neural Stem Cells (NSC). The adhesion and the arrangement of the stem cells were analyzed to verify the ability of the patterned substrates to guide the orientation and, therefore, the differentiation of the cells

Process parameters effect on mechanical properties and fatigue behaviour of friction stir weld AA6060 joints

Friction stir welding (FSW) is the most remarkable welding technology that has been invented and developed in the last decade. It is a solid-state welding process in which a rotating tool is driven into the material and translated along the interface of two or more plates. This technology has been successfully used to join materials that are considered difficult to be welded by fusion welding methods. FSW has potentially significant applications in many industrial fields such as aerospace, automotive, and naval industry. Anyway, FSW technology requires a meticulous understanding of the process and consequent mechanical properties of the welds in order to be used in the production of high performance components. The present work deals with an experimental campaign aimed at the evaluation of the mechanical properties of AA6060 T6 friction stir welded joints. The butt joints obtained using two different tool geometries (standard and threaded) were performed by varying the welding parameters, namely, tool rotating speed and feed rate. The standard tool was a very simple device fabricated using AISI 1040 steel, with a flat shoulder and a cylindrical pin. The threaded tool was a more complex device based on two main components: a tool holder, with a flat shoulder, and a threaded probe obtained using a commercial thread forming tap. The quality of the joints was evaluated in terms of both tensile strength (UTS) and fatigue behavior. The study of axial pulsing fatigue properties required the fabrication of a specific testing device able to avoid parasite bending moments. In order to estimate the more efficient and effective tool type, the welding forces (axial and longitudinal) were also measured

Influence of Temperature on Mutagenicity in Plants Exposed to Surface Disinfected Drinking Water.

Disinfection of surface drinking water, particularly water chlorination, produces by-products with potential genotoxic and/or carcinogenic activity. A study carried out at a pilot plant for drinking water disinfection of lake water revealed mutagenic activity of three different disinfectants (sodium hypochlorite, chlorine dioxide and peracetic acid) in different seasons using in situ mutagenicity assays, both in animal (micronucleus test) and in plant organisms (anaphase chro- mosomal aberration and micronucleus tests). The effects of the disinfectants appeared to be modulated by the season of exposure. In this study, we tried to understand if (and to what extent) the temperature parameter could actually play an independent role in the registered seasonal variation of mutagenic effects, neglecting the variation of other parameters, e.g. physical conditions and chemical composition of the lake water. Therefore plants (Allium cepa for chromosomal aberration test and Vicia faba for micronucleus test) were exposed to the same disinfected lake-water samples at differ- ent temperatures (10°C, 20°C and 30°C), according the ones registered during the in situ experiment. Long-term expo- sure at the temperatures of 20°C (both Vicia faba and Allium cepa) and 30°C (Vicia faba only) to disinfected waters in- duced clear mutagenic effects. These results show that temperature is an important variable which should be taken into account when in situ exposure of plants is planned for mutagenicity testing. Also, different plant systems clearly show specific temperature ranges suitable for their growth, thereby indicating the need for an accurate selection of the test organism for a specific experimental plan

Transition metal b-site substitutions in laalo3 perovskites reorient bio-ethanol conversion reactions

LaAlO3 perovskites, as such and with 25% molar Al substitution by Cu, Co, or Ga, have been prepared by sol-gel methods and tested as heterogeneous catalysts in the gas-phase conversion of ethanol. LaAlO3 presented a significant acidic character, with high formation of ethylene by ethanol dehydration. B-site substitutions increased the basicity of the catalysts, favoring the dehydrogenation of ethanol to acetaldehyde. The most reducible Cu-and Co-substituted materials, characterized by easier formation of surface oxygen vacancies, promoted the self-condensation of acetaldehyde by the Tishchenko mechanism, with formation of acetone and odd-carbon number products. Aldol coupling of acetaldehyde, favored on pure and Ga-substituted LaAlO3, led to the formation of butadiene and hexadiene. The role of Ga insertion, favoring both dehydrogenation of ethylene and dehydration of higher alcohols, corresponds to an amphoteric character. The formation of olefins and diolefins on all catalysts suggests that LaAl-based materials present the most acidic character among La-perovskites

Laparoscopic splenectomy for splenic hamartoma : a case report

Hamartoma is a rare splenic benign tumor usually accidentally detected as a radiologic finding. Preoperative diagnosis poses a challenge and thus surgery becomes necessary to confirm the clinical suspicion. Laparoscopic splenectomy has gained consensus as a standard surgical procedure particularly for autoimmune hematological diseases. This former experience has allowed this technique to be extended to other splenic pathologies. Here we report a case of total laparoscopic splenectomy for a bulky splenic hamartoma in a young male patien

The Great Pretender : Rectal Syphilis Mimic a Cancer

Rectal syphilis is a rare expression of the widely recognised sexual transmitted disease, also known as the great imitator for its peculiarity of being confused with mild anorectal diseases because of its vague symptoms or believed rectal malignancy, with the concrete risk of overtreatment. We present the case of a male patient with primary rectal syphilis, firstly diagnosed as rectal cancer; the medical, radiological, and endoscopic features are discussed below

Laparoscopic colonic resection for splenic flexure cancer: our experience

Background: The treatment of colon cancer located in splenic flexure is not standardized. Laparoscopic approach is still considered a challenging procedure. This study reviews two Institutions experience in laparoscopic treatment of left colonic flexure cancer. Intraoperative, pathologic and postoperative data from patients undergoing laparoscopic splenic flexure resection were analyzed to assess oncological safety as well as early and medium-term outcomes. Methods: From October 2005 to May 2014 laparoscopic splenic flexure resection was performed in 23 patients. Results: Conversion rate was nihil. In 7 cases the anastomosis was performed intracorporeally. Specimen mean length was 21.2 cm, while the distance of distal and proximal resection margin from tumor site was 6.5 and 11.5 respectively. The mean number of harvested lymph nodes was 20.8. Mean operative time was 190 min and mean estimated blood loss was equal to 55 ml. As regard major postoperative complications, one case of postoperative acute pancreatitis and one case of postoperative bleeding from the anastomotic suture line were reported. Conclusions: Although our experience is limited and appropriate indications must be set by future randomized studies, we believe that laparoscopic resection with intracorporeal anastomosis appears feasible and safe for patients affected by splenic flexure cancer

Laser decoating of DLC films for tribological applications

Damaged DLC coatings usually require remanufacturing of the entire coated components starting from an industrial chemical de-coating step. Alternatively, a complete or local coating repair can be considered. To pursue this approach, however, a local coating removal is needed as first operation. In this context, controlled decoating based on laser sources can be a suitable and clean alternative to achieve a pre-fixed decoating depth with high accuracy. In the present study, we investigated a laser-based decoating process executed on multilayered DLC films for advanced tribological applications (deposited via a hybrid PVD/PE-CVD technique). The results were acquired via multifocal optical digital microscopy (MF-ODM), which allowed high-resolution 3D surface reconstruction as well as digital profilometry of the lasered and unlasered surface. The study identifies the most critical process parameters which influence the effective decoating depth and the post-decoating surface roughness. In particular, the role of pulse overlap (decomposed along orthogonal directions), laser fluence, number of lasing passes and assist gas is discussed in text. A first experimental campaign was designed to identify the best conditions to obtain full decoating of the DLC + DLC:Cr layers. It was observed that decreasing the marking speed to 200 mm/s was necessary to obtain a sufficient pulse overlap and a nearly planar ablation profile. By operating with microsecond pulses and 1 J/cm2 (fairly above the ablation threshold), less than 10 passes were needed to obtain full decoating of the lasered area with an etching rate of 1.1 μm/loop. Further experiments were then executed in order to minimise the roughness of the rest surface with the best value found at around 0.2 μm. Limited oxidation but higher Ra values were observed in Ar atmosphere