910 research outputs found
MicroRNA-222 regulates muscle alternative splicing through Rbm24 during differentiation of skeletal muscle cells
A number of microRNAs have been shown to regulate skeletal muscle development and differentiation. MicroRNA-222 is downregulated during myogenic differentiation and its overexpression leads to alteration of muscle differentiation process and specialized structures. By using RNA-induced silencing complex (RISC) pulldown followed by RNA sequencing, combined with in silico microRNA target prediction, we have identified two new targets of microRNA-222 involved in the regulation of myogenic differentiation, Ahnak and Rbm24. Specifically, the RNA-binding protein Rbm24 is a major regulator of muscle-specific alternative splicing and its downregulation by microRNA-222 results in defective exon inclusion impairing the production of muscle-specific isoforms of Coro6, Fxr1 and NACA transcripts. Reconstitution of normal levels of Rbm24 in cells overexpressing microRNA-222 rescues muscle-specific splicing. In conclusion, we have identified a new function of microRNA-222 leading to alteration of myogenic differentiation at the level of alternative splicing, and we provide evidence that this effect is mediated by Rbm24 protei
Preliminary study of feasibility of an experiment looking for excited state double beta transitions in tin
An attempt to study the feasibility of a new experiment to search for double
beta decay in Sn and Sn was carried out by using ultra-low
background HPGe detector (244 cm) inside the Gran Sasso National
Laboratory (LNGS) of the INFN (Italy). A small sample of natural Sn was
examined for 2367.5 h. The radioactive contamination of the sample has been
estimated. The data has also been considered to calculate the present
sensitivity for the proposed search; half-life limits years for EC and EC-EC processes in Sn and
years for transition in Sn were
measured. In the last section of the paper the enhancement of the sensitivity
for a proposed experiment with larger mass to reach theoretically estimated
values of half-lives is discussed.Comment: 20 pages, 11 figures, 4 tables, accepted for publication in NIMA (in
press
New limits on , and decay of Ce and Ce with deeply purified cerium sample
A search for double electron capture (), electron capture with
positron emission (), and double positron emission
) in Ce and Ce was realized with a 465 cm
ultra-low background HP Ge spectrometer over 2299 h at the Gran Sasso
underground laboratory. A 627 g sample of cerium oxide deeply purified by
liquid-liquid extraction method was used as a source of quanta
expected in double decay of the cerium isotopes. New improved half-life
limits were set on different modes and channels of double decay of
Ce and Ce at the level of yr.Comment: 19 pages, 6 figures, 2 table
DAMA/NaI results
The DAMA/NaI set-up of the DAMA experiment has been operative during seven
annual cycles and has investigated several rare processes. In particular, it
has been realised in order to investigate the model independent annual
modulation signature for Dark Matter particles in the galactic halo. With the
total exposure collected in the seven annual cycles (107731 kg day) a model
independent evidence for the presence of a Dark Matter particle component in
the galactic halo has been pointed out at 6.3 sigma C.L.. Some of the many
possible corollary model dependent quests for the candidate particle have been
presented as well.Comment: Contributed paper to the Rencontres de Moriond "Electroweak
Interactions and Unified Theories", La Thuile, Aosta Valley, Italy, March
200
Histomorphometric analysis of osteocyte lacunae in human and pig: exploring its potential for species discrimination
In recent years, several studies have focused on species discrimination of bone fragments by histological analysis. According to literature, the most consistent distinguishing features are Haversian canal and Haversian system areas. Nonetheless, there is a consistent overlap between human and non-human secondary osteon dimensions. One of the features that have never been analyzed for the purpose of species discrimination is the osteocyte lacuna, a small oblong cavity in which the osteocyte is locked in. The aim of this study is to verify whether there are significant quantitative differences between human and pig lacunae within secondary osteons with similar areas. Study sample comprises the midshaft of long bones (humerus, radius, ulna, femur, tibia, and fibula) of a medieval human adult and a juvenile pig. Sixty-eight secondary osteons with similar areas have been selected for each species and a total of 1224 osteocyte lacunae have been measured. For each osteon, the total number of lacunae was counted, and the following measurements were taken: minimum and maximum diameter, area, perimeter, and circularity of nine lacunae divided between inner, intermediate, and outer lacunae. Statistical analysis showed minimal differences between human and pig in the number of lacunae per osteons and in the minimum diameter (P > 0.05). On the contrary, a significant difference (P < 0.001) has been observed in the maximum diameter, perimeter, area, and circularity. Although there is the need for further research on different species and larger sample, these results highlighted the potential for the use of osteocyte lacunae as an additional parameter for species discrimination. Concerning the difference between the dimensions of osteocyte lacunae based on their position within the osteon (inner, intermediate, and outer lacunae), results showed that their size decreases from the cement line towards the Haversian canal both in human and pig
The Many Facets of Genetic Literacy: Assessing the Scalability of Multiple Measures for Broad Use in Survey Research
Objectives
To determine how three dimensions of genetic literacy (familiarity, skills, and factual knowledge) fit the hierarchy of knowledge outlined in E.M. Rogers’ Diffusion of Innovations to better conceptualize lay understandings of genomics. Methods
A consumer panel representing the US adult population (N = 1016) completed an electronic survey in November 2013. Adjusting for education, we used correlations, principle components analysis, Mokken Scale tests, and linear regressions to assess how scores on the three genetic literacy sub-dimensions fit an ordered scale. Results
The three scores significantly loaded onto one factor, even when adjusting for education. Analyses revealed moderate strength in scaling (0.416, p\u3c0.001) and a difficulty ordering that matched Rogers’ hierarchy (knowledge more difficult than skills, followed by familiarity). Skills scores partially mediated the association between familiarity and knowledge with a significant indirect effect (0.241, p\u3c0.001). Conclusion
We established an ordering in genetic literacy sub-dimensions such that familiarity with terminology precedes skills using information, which in turn precedes factual knowledge. This ordering is important to contextualizing previous findings, guiding measurement in future research, and identifying gaps in the understanding of genomics relevant to the demands of differing applications
- …