2,356 research outputs found
Cellular and mucosal immune reactions to mental and cold stress: Associations with gender and cardiovascular reactivity
To examine gender differences in immune reactions to stress and relationships between immune and cardiovascular reactivity, measures of cellular and mucosal immunity and cardiovascular activity were recorded in 77 men and 78 women at rest and in response to active (mental arithmetic) and passive (cold pressor) stress tasks. Both tasks reduced CD4+ T cells and the CD4/8 ratio. Total lymphocytes, NK cells, CD8+ T cells, and secretory immunoglobulin A (sIgA) increased with active stress. Passive stress decreased sIgA. At rest, men had more NK cells, less CD4+ T cells, and fewer neutrophils than women. Mental stress increased sIgA in men but not women. Cardiovascular reactivity to active stress was associated with increases in NK cells. The data support the hypothesis that stress-related increases in lymphocytes are beta-adrenergically mediated, and suggest that the fall in CD4+ T cells may be alpha-adrenergically driven. Mechanisms underlying sIgA reactions are more difficult to determine. Men and women differed in some cell counts, but not in reactivity, although gender influenced sIgA reactions to arithmetic
Moved by the tears of others: emotion networking in the heritage sphere
There is no heritage without emotional sharing and clashing. This article explores the involvement of divergent emotions in heritage making by discussing the debate series of Imagine IC and the Reinwardt Academy and zooming in on the commemoration of slavery and imagery of ‘Black Pete’ in the Netherlands. We introduce ‘emotion networking’ as a methodology to approach present-day heritage production, aiming for a novel approach to engage with ‘the collective’
Analysis of medium resolution spectra by automated methods - application to M55 and omega Centauri
We have employed feedforward neural networks trained on synthetic spectra in
the range 3800 to 5600 AA with resolutions of 2-3 AA to determine metallicities
from spectra of about 1000 main-sequence turn-off, subgiant and red giant stars
in the globular clusters M55 and omega Cen. The overall metallicity accuracies
are of the order of 0.15 to 0.2 dex. In addition, we tested how well the
stellar parameters logg and Teff can be retrieved from such data without
additional colour or photometric information. We find overall uncertainties of
0.3 to 0.4 dex for logg and 140 to 190 K for Teff. In order to obtain some
measure of uncertainty for the determined values of [Fe/H], logg and Teff, we
applied the bootstrap method for the first time to neural networks for this
kind of parametrization problem. The distribution of metallicities for stars in
omega Cen clearly shows a large spread in agreement with the well known
multiple stellar populations in this cluster.Comment: 13 pages, 9 figures, accepted for publication in A&
Critical State Flux Penetration and Linear Microwave Vortex Response in YBa_2Cu_3O_{7-x} Films
The vortex contribution to the dc field (H) dependent microwave surface
impedance Z_s = R_s+iX_s of YBa_2Cu_3O_{7-x} thin films was measured using
suspended patterned resonators. Z_s(H) is shown to be a direct measure of the
flux density B(H) enabling a very precise test of models of flux penetration.
Three regimes of field-dependent behavior were observed: (1) Initial flux
penetration occurs on very low field scales H_i(4.2K) 100Oe, (2) At moderate
fields the flux penetration into the virgin state is in excellent agreement
with calculations based upon the field-induced Bean critical state for thin
film geometry, parametrized by a field scale H_s(4.2K) J_c*d 0.5T, (3) for very
high fields H >>H_s, the flux density is uniform and the measurements enable
direct determination of vortex parameters such as pinning force constants
\alpha_p and vortex viscosity \eta. However hysteresis loops are in
disagreement with the thin film Bean model, and instead are governed by the low
field scale H_i, rather than by H_s. Geometric barriers are insufficient to
account for the observed results.Comment: 20 pages, LaTeX type, Uses REVTeX style files, Submitted to Physical
Review B, 600 dpi PostScript file with high resolution figures available at
http://sagar.physics.neu.edu/preprints.htm
Protein methylation is required to maintain optimal HIV-1 infectivity
BACKGROUND: Protein methylation is recognized as a major protein modification pathway regulating diverse cellular events such as protein trafficking, transcription, and signal transduction. More recently, protein arginine methyltransferase activity has been shown to regulate HIV-1 transcription via Tat. In this study, adenosine periodate (AdOx) was used to globally inhibit protein methyltransferase activity so that the effect of protein methylation on HIV-1 infectivity could be assessed. RESULTS: Two cell culture models were used: HIV-1-infected CEM T-cells and HEK293T cells transfected with a proviral DNA plasmid. In both models, AdOx treatment of cells increased the levels of virion in culture supernatant. However, these viruses had increased levels of unprocessed or partially processed Gag-Pol, significantly increased diameter, and displayed reduced infectivity in a MAGI X4 assay. AdOx reduced infectivity equally in both dividing and non-dividing cells. However, infectivity was further reduced if Vpr was deleted suggesting virion proteins, other than Vpr, were affected by protein methylation. Endogenous reverse transcription was not inhibited in AdOx-treated HIV-1, and infectivity could be restored by pseudotyping HIV with VSV-G envelope protein. These experiments suggest that AdOx affects an early event between receptor binding and uncoating, but not reverse transcription. CONCLUSION: Overall, we have shown for the first time that protein methylation contributes towards maximal virus infectivity. Furthermore, our results also indicate that protein methylation regulates HIV-1 infectivity in a complex manner most likely involving the methylation of multiple viral or cellular proteins and/or multiple steps of replication
Alternative Markers of Performance in Simulation: Where We Are and Where We Need To Go
This article on alternative markers of performance in simulation is the product of a session held during the 2017 Academic Emergency Medicine Consensus Conference â Catalyzing System Change Through Health Care Simulation: Systems, Competency, and Outcomes.â There is a dearth of research on the use of performance markers other than checklists, holistic ratings, and behaviorally anchored rating scales in the simulation environment. Through literature review, group discussion, and consultation with experts prior to the conference, the working group defined five topics for discussion: 1) establishing a working definition for alternative markers of performance, 2) defining goals for using alternative performance markers, 3) implications for measurement when using alternative markers, identifying practical concerns related to the use of alternative performance markers, and 5) identifying potential for alternative markers of performance to validate simulation scenarios. Five research propositions also emerged and are summarized.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/142535/1/acem13321_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/142535/2/acem13321.pd
Owning an overweight or underweight body: distinguishing the physical, experienced and virtual body
Our bodies are the most intimately familiar objects we encounter in our perceptual environment. Virtual reality provides a unique method to allow us to experience having a very different body from our own, thereby providing a valuable method to explore the plasticity of body representation. In this paper, we show that women can experience ownership over a whole virtual body that is considerably smaller or larger than their physical body. In order to gain a better understanding of the mechanisms underlying body ownership, we use an embodiment questionnaire, and introduce two new behavioral response measures: an affordance estimation task (indirect measure of body size) and a body size estimation task (direct measure of body size). Interestingly, after viewing the virtual body from first person perspective, both the affordance and the body size estimation tasks indicate a change in the perception of the size of the participant’s experienced body. The change is biased by the size of the virtual body (overweight or underweight). Another novel aspect of our study is that we distinguish between the physical, experienced and virtual bodies, by asking participants to provide affordance and body size estimations for each of the three bodies separately. This methodological point is important for virtual reality experiments investigating body ownership of a virtual body, because it offers a better understanding of which cues (e.g. visual, proprioceptive, memory, or a combination thereof) influence body perception, and whether the impact of these cues can vary between different setups
Microwave Current Imaging in Passive HTS Components by Low-Temperature Laser Scanning Microscopy (LTLSM)
We have used the LTLSM technique for a spatially resolved investigation of
the microwave transport properties, nonlinearities and material inhomogeneities
in an operating coplanar waveguide YBa_2Cu_3O_{7-\delta} (YBCO) microwave
resonator on an LaAlO_3 (LAO) substrate. The influence of twin-domain blocks,
in-plane rotated grains, and micro-cracks in the YBCO film on the nonuniform rf
current distribution were measured with a micrometer-scale spatial resolution.
The impact of the peaked edge currents and rf field penetration into weak links
on the linear device performance were studied as well. The LTLSM capabilities
and its future potential for non-destructive characterization of the microwave
properties of superconducting circuits are discussed.Comment: 8 pages, 9 figures, 2-column format, presented at High Temperature
Superconductors in High Frequency Fields 2004, Journal of Superconductivity
(in press
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