Decolonizing Ecology Through Rerooting Epistemologies

My project is centered around a community garden in Upland, California called the People and Their Plants garden. This garden represents a five hundred year living history designed to show the changes in the ecological landscape of Southern California caused by colonization. This autoethnographic thesis works towards personal, interpersonal, and community-wide decolonization through building reciprocal relationships with Indigenous Elders. I explore, critique and problematize research and ethnography by examining the politics of knowledge, language, history, and ecology. I interrogate my own learned knowledge systems as well as colonial/capitalist food systems—and recognize how those systems/relations have worked to render Indigenous ways of knowing as invisible. Furthermore, I examine the connection between colonialism, gender, and capitalist food systems. I explain how the People and Their Plants garden is an act of resistance to colonial/capitalist food systems as it creates space for alternative economic practices and decolonial food practices. As part of this project, I co-authored a brochure about the garden with a Tongva Elder

Engineering Yeast to Evaluate Human Proteins Involved in Selective RNA Packaging During HIV Particle Production

Despite recent advances in antiretroviral therapy, nearly 37 million people continue to live with human immunodeficiency virus (HIV). Basic and applied research on the assembly of HIV could be enhanced by using a genetically tractable organism, such as yeast, rather than mammalian cells. While previous studies showed that expression of the HIV Gag polyprotein in Saccharomyces cerevisiae spheroplasts resulted in the production of virus-like particles (VLPs), many questions regarding the utility of yeast in HIV assembly remain uninvestigated. Here, we report use of S. cerevisiae for both the production of VLPs with selectively packaged RNA and to evaluate the human Y-box-binding protein 1 (YB-1) in selective RNA packaging into VLPs. Our data reveal: (1) When co-expressed alongside HIV-1 Gag, an RNA mammalian expression cassette is selectively encapsidated and released in VLPs produced from spheroplasts; (2) Inclusion of the 5’UTR-5’Gag RNA upstream of the mammalian expression cassette greatly increased the selectivity to which non-viral RNA was packaged into VLPs; and (3) heterologous expression of the human YB-1 protein in S. cerevisiae did not facilitate the selective packaging of viral RNA into VLPs, likely due to inability to bind upstream elements in the HIV-1 viral RNA. Overall, this research provides a key first step in the use of yeast for the production of viral vectors used in gene therapy, and lays a foundation for further experiments investigating the role of YB-1 and other host proteins in selective RNA packaging

Generation of monochromatic electrostatic waves of large amplitude in a bounded beam-plasma system

Monochromatic electrostatic waves of large amplitude were excited by the interaction of an electron beam with a bounded plasma. These waves were identified as resonant beam modes, which are amplified by multiple reflexion in a cavity. Nonlinear effects, such as the generation of harmonies and sidebands, were observe

Supporting knowledge elicitation for learning in virtual teams

The growing complexity and dynamics of professional work increasingly require teamwork. Continuous learning while working will be obligatory to meet the performance requirements of the workplace. In this context asynchronous collaboration becomes more common and poses new educational design questions. Many questions regarding these new ways of working and learning are yet to be resolved. One pivotal issue is how to effectively support eliciting and sharing available but not yet articulated knowledge residing in the minds of individual team members. Suggestions derived from literature about knowledge elicitation point in different directions. In order to investigate knowledge elicitation support for professionals in virtual teams, an electronic Delphi study was executed. The objective was to gain insights regarding knowledge elicitation from a group of 16 representative experts. The results reveal the importance of customising multiple aspects to the specific situation. Each context requires a mixture of team, knowledge awareness and task related prompts. Based upon generic know-how with respect to enabling virtual team dynamics and community formation, social and task related knowledge prompts should be dedicated to the constraints and dynamics of the organisational context

Structure and Function of the Mycobacterial Type VII Secretion Systems

Bacteria have evolved intricate secretion machineries for the successful delivery of large molecules across their cell envelopes. Such specialized secretion systems allow a variety of bacteria to thrive in specific host environments. In mycobacteria, type VII secretion systems (T7SSs) are dedicated protein transport machineries that fulfill diverse and crucial roles, ranging from metabolite uptake to immune evasion and subversion to conjugation. Since the discovery of mycobacterial T7SSs about 15 y ago, genetic, structural, and functional studies have provided insight into the roles and functioning of these secretion machineries. Here, we focus on recent advances in the elucidation of the structure and mechanism of mycobacterial T7SSs in protein secretion. As many of these systems are essential for mycobacterial growth or virulence, they provide opportunities for the development of novel therapies to combat a number of relevant mycobacterial diseases

Alternative optical concept for electron cyclotron emission imaging

The implementation of advanced electron cyclotron emission imaging (ECEI) systems on tokamak experiments has revolutionized the diagnosis of magnetohydrodynamic (MHD) activities and improved our understanding of instabilities, which lead to disruptions. It is therefore desirable to have an ECEI system on the ITER tokamak. However, the large size of optical components in presently used ECEI systems have, up to now, precluded the implementation of an ECEI system on ITER. This paper describes a new optical ECEI concept that employs a single spherical mirror as the only optical component and exploits the astigmatism of such a mirror to produce an image with one-dimensional spatial resolution on the detector. Since this alternative approach would only require a thin slit as the viewing port to the plasma, it would make the implementation of an ECEI system on ITER feasible. The results obtained from proof-of-principle experiments with a 125 GHz microwave system are presented. (C) 2014 AIP Publishing LLC

Iron impregnation on the amorphous shell of vapor grown carbon fibers and the catalytic growth of secondary nanofibers

Vapor grown carbon fibers (VGCFs) with diameters of several microns were synthesized and investigated by high resolution transmission electron microscopy. It was found that the shell of the VGCFs consisted of densely-packed domains embedded in loosely-packed matrix, and both were highly amorphous. Regular edge planes as observed on the surface of fishbone nanofibers do not exist on VGCFs. Hence, surface treatment is more important for the deposition of catalysts. Ammonium ferric citrate (AFC) was employed for the impregnation of iron, where the high viscosity of the aqueous solution of AFC is beneficial. Calcination was found to be a key step to improve the dispersion of the iron particles, which can be attributed to enhanced interactions between iron and carbon due to the gasification of carbon occurring at the iron-carbon interface. Quantitative analysis by X-ray photoelectron spectroscopy showed that the calcination of the supported AFC led to a higher atomic concentration of iron on the surface, indicating smaller particle size and higher dispersion. Secondary carbon nanofibers were grown subsequently on the VGCFs from cyclohexane. The specific surface area was enhanced considerably, from less than 1 m2 g-1 to 106 m2 g-1 after the growth of the secondary nanofibers. The obtained composites are promising materials as structured support in heterogeneous catalysis

Coherent Ro-vibrational Revivals in a Thermal Molecular Ensemble

We report an experimental and theoretical study of the evolution of vibrational coherence in a thermal ensemble of nitrogen molecules. Rotational dephasing and rephasing of the vibrational coherence is detected by coherent anti-Stokes Raman scattering. The existence of ro-vibrational coupling and the discrete energy spectrum of the rotational bath lead to a whole new class of full and fractional ro-vibrational revivals. Following the rich ro-vibrational dynamics on a nanosecond time scale with sub-picosecond time resolution enables us to determine the second-order ro-vibrational constant $gamma_e$ and assess new possibilities of controlling decoherence.Comment: submitted at Physical Review