Another Look at Confidence Intervals: Proposal for a More Relevant and Transparent Approach

The behaviors of various confidence/credible interval constructions are explored, particularly in the region of low statistics where methods diverge most. We highlight a number of challenges, such as the treatment of nuisance parameters, and common misconceptions associated with such constructions. An informal survey of the literature suggests that confidence intervals are not always defined in relevant ways and are too often misinterpreted and/or misapplied. This can lead to seemingly paradoxical behaviours and flawed comparisons regarding the relevance of experimental results. We therefore conclude that there is a need for a more pragmatic strategy which recognizes that, while it is critical to objectively convey the information content of the data, there is also a strong desire to derive bounds on models and a natural instinct to interpret things this way. Accordingly, we attempt to put aside philosophical biases in favor of a practical view to propose a more transparent and self-consistent approach that better addresses these issues.Comment: 23 pages, 11 figure

Detection of Bacterial Lipopolysaccharides of Escherichia coli by Direct Analysis in Real Time/Time-of-Flight Mass Spectrometry

Lipopolysaccharides (LPS) are a major component of the outer membrane in Gram-negative bacteria species and are responsible for toxicity caused by organisms such as Escherichia coli (E.coli). Conventional methods for characterizing LPS utilize mass spectrometry, particularly matrix assisted laser desorption ionization mass spectrometry (MALDI-MS) and liquid chromatography mass spectrometry (LC-MS). Although these are successful in analyzing intact LPS molecules, they can be time-consuming. Other mass spectrometry techniques, such as direct analysis in real time/time-of-flight mass spectrometry (DART-TOF-MS) could also be helpful in the rapid analysis of LPS molecules without sample extraction procedures. This research focuses on the application of a DART-TOF-MS method to rapidly identify LPS molecules from E. coli as well as an analysis of LPS extracts generated using a commercial LPS extraction kit by gas chromatography-flame ionization detection (GC-FID). It was determined that DART-TOF-MS allows for the direct detection of LPS-specific fatty acids from solutions containing intact E.coli cells. Finally, the GC-FID based evaluation of the commercial LPS extraction kit showed that resulting extracts were enriched in LPS-specific fatty acids compared to intracellular/membrane fatty acids

The First Direct Distance and Luminosity Determination for a Self-Luminous Giant Exoplanet: The Trigonometric Parallax to 2MASS1207334-393254Ab

We present the first trigonometric parallax and distance for a young planetary mass object. A likely TW Hya cluster member, 2MASSW J1207334-393254Ab (hereafter 2M1207Ab) is an M8 brown dwarf with a mid to late L type planetary mass companion. Recent observations of spectral variability have uncovered clear signs of disk accretion and outflow, constraining the age of the system to <10 Myr. Because of its late spectral type and the clearly youthful nature of the system, 2M1207b is very likely a planetary mass object. We have measured the first accurate distance and luminosity for a self-luminous planetary mass object. Our parallax measurements are accurate to <2 mas (1sigma) for 2M1207Ab. With 11 total epochs of data taken from January 2006 through April 2 007 (475 images for 2M1207Ab), we determine a distance of 58.8+-7.0 pc (17.0{+2.3}{-1.8} mas, 1.28sigma) to 2M1207Ab and a calculated luminosity of 0.68-2.2x10^-5 Lsun for 2M1207b. Hence 2M1207Ab is a clear member of the TW Hya cluster in terms of its distance, proper motions, and youthful nature. However, as previously noted by Mohanty and co-workers, 2M1207b's luminosity appears low compared to its temperature according to evolutionary models.Comment: 12 pages, 3 figures, accepted to ApJ Letter

Informed Consent - Balance zwischen Patientenschutz und Forschungsinteressen

Forschungsethische Debatten haben sich in den letzten Jahren häufig der Frage gewidmet, ob Ausnahmen bei der strikten Forderung nach individueller Einwilligung des Patienten zulässig oder gar geboten sind. Einige Studiendesigns mit solchen Ausnahmeregelungen werden im Folgenden zur Diskussion vorgestellt

The very nearby M/T dwarf binary SCR 1845-6357

The recently discovered star SCR 1845-6357 is the first late M/T dwarf binary discovered. SCR 1845 is a particular object due to its tight orbit (currently around 4 AU) and its proximity to the Sun (3.85 pc). We present spatially resolved VLT/NACO images and low resolution spectra of SCR 1845 in the J, H and K near-infrared bands. Since the T dwarf companion, SCR 1845B, is so close to the primary SCR 1845A, orbital motion is evident even within a year. Following the orbital motion, the binary's mass can be measured accurately within a decade, making SCR 1845B a key T-dwarf mass-luminosity calibrator. The NIR spectra allow for accurate determination of spectral type and also for rough estimates of the object's physical parameters. The spectral type of SCR 1845B is determined by direct comparison of the flux calibrated JHK spectra with T dwarf standard template spectra and also by NIR spectral indices obtained from synthetic photometry. Constrained values for surface gravity, effective temperature and metallicity are derived by comparison with model spectra. Our data prove that SCR 1845B is a brown dwarf of spectral type T6 that is co-moving with and therefore gravitationally bound to the M8.5 primary. Fitting the NIR spectrum of SCR 1845B to model spectra yields an effective temperature of about 950K and a surface gravity log(g)=5.1 (cgs) assuming solar metallicity. Mass and age of SCR 1845B are in the range 40 to 50 Jupiter masses and 1.8 to 3.1 Gyr.Comment: 5 pages, 4 figures, accepted for publication in Astronomy & Astrophysic

Complications of Cushing's syndrome: state of the art

Cushing's syndrome is a serious endocrine disease caused by chronic, autonomous, and excessive secretion of cortisol. The syndrome is associated with increased mortality and impaired quality of life because of the occurrence of comorbidities. These clinical complications include metabolic syndrome, consisting of systemic arterial hypertension, visceral obesity, impairment of glucose metabolism, and dyslipidaemia; musculoskeletal disorders, such as myopathy, osteoporosis, and skeletal fractures; neuropsychiatric disorders, such as impairment of cognitive function, depression, or mania; impairment of reproductive and sexual function; and dermatological manifestations, mainly represented by acne, hirsutism, and alopecia. Hypertension in patients with Cushing's syndrome has a multifactorial pathogenesis and contributes to the increased risk for myocardial infarction, cardiac failure, or stroke, which are the most common causes of death; risks of these outcomes are exacerbated by a prothrombotic diathesis and hypokalaemia. Neuropsychiatric disorders can be responsible for suicide. Immune disorders are common; immunosuppression during active disease causes susceptibility to infections, possibly complicated by sepsis, an important cause of death, whereas immune rebound after disease remission can exacerbate underlying autoimmune diseases. Prompt treatment of cortisol excess and specific treatments of comorbidities are crucial to prevent serious clinical complications and reduce the mortality associated with Cushing's syndrome

Memory Loss, Behavioral Changes, and Slurred Speech in a 49-Year-Old Man

A 49-year-old man presented with slurred speech, memory loss, and behavioral changes. His clinical course was marked by decline in functional status and cognition. He had poorly controlled hypertension and hyperlipidemia. Neuroimaging was remarkable for multiple subcortical white matter lesions. We discuss the diagnostic and therapeutic approach of rapidly progressing cognitive decline in the given clinical setting