AGENTES ASOCIADOS Y SU PAPEL EN LA DECLINACIÓN Y MUERTE DE ENCINOS (QUERCUS, FAGACEAE) EN EL CENTRO-OESTE DE MÉXICO

This study was carried out to identify bioticand abiotic agents and to determine theirrole in oak decline and death in five statesof the Mexican Republic: Aguascalientes,Colima, Guanajuato, Jalisco and Nayarit.Two 0.1-ha permanent plots wereestablished at the most representativeforested areas with tree death, diebackand bleeding cankers in these statesand sampled during 2003, 2004 and 2005(10 plots in total). Field observations weremade, and foliage, branch, trunk and soilsamples were collected to identify theassociated agents in each plot. Isolates frombleeding cankers were made in situ. Themost commonly found biotic agents were:Phytophthora cinnamomi, Pythium sp.,Hypoxylon antropunctatum, Ganodermaspp., Armillaria sp., Apiognomoniaquercina, the dwarf mistletoe(Phoradendron villosum), bark borers,insect defoliators and livestock overgrazing.Among the abiotic stress factors commonlyfound were the effects due to lowtemperature-lack of water and fire. Basedon their incidence, P. cinnamomi, H.antropunctatum and low temperature-lackof water were considered the most importantcontributors to the decline and death of theoaks. P. cinnamomi was identified in four ofthe 10 plots, mainly in Colima and Jalisco;H. antropunctatum was detected in sixplots, particularly in Nayarit andAguascalientes. Stem cankers (cracks) dueto low temperature-lack of water wereobserved in four plots, damaging 52% of the trees in Aguascalientes and 24% inGuanajuato. It was concluded that P.cinnamomi is killing some oak species, H.antropunctatum is colonizing weakenedtrees, and low temperature-lack of water iscausing stress and in some cases death inoaks.Este estudio tuvo como objetivo identificary determinar el papel tanto de los factoresbióticos como de los abióticos asociados ala declinación y muerte del encino en cincoestados de la República mexicana: Aguascalientes, Colima, Guanajuato, Jalisco yNayarit. Para esto, se establecieron dossitios permanentes de 0.1 ha en cada una delas áreas más representativas (con arboladomuerto, con muerte regresiva y cancros conexudado) de los estados mencionados (10sitios en total), los cuales fueron muestreados durante 2003, 2004 y 2005. Sehicieron observaciones y colectas en campode follaje, ramas, tronco y suelo, paraidentificar a los diferentes agentes de dañoen cada sitio. Se realizaron aislamientosin situ de cancros con exudado en mediosespecíficos. Los agentes bióticos máscomunes fueron: Phytophthora cinnamomi,Pythium sp., Hypoxylon antropunctatum,Ganoderma sp., Armillaria sp.,Apiognomonia quercina, el muérdagoenano (Phoradendron villosum), barrenadores, defoliadores y sobrepastoreo. Entre los abióticos causantes de estrés ymuerte, destacaron los daños ocasionadospor las bajas temperaturas-falta de aguay fuego. Con base en su incidencia, P.cinnamomi, H. antropunctatum y las bajastemperaturas-falta de agua son considerados los factores de mayor importanciaen la declinación y muerte del encino. P.cinnamomi fue identificado en cuatro delos diez sitios, particularmente de Colimay Jalisco; H. antropunctatum fué detectadoen seis de diez, principalmente en losrodales de Nayarit y Aguascalientes. Loscancros de troncos (rajaduras), debido a lasbajas temperaturas-falta de agua, seobservaron en cuatro sitios, afectando al52% de los árboles en Aguascalientes y24% en Guanajuato. Se concluye que P.cinnamomi está ocasionando la muertede algunas especies de encino, H.antropunctatum está coloni-zando árbolesdebilitados y las bajas temperaturas-faltade agua están participando como agentescausantes de estrés y muerte en algunoscasos

Carbapenem-resistant Citrobacter spp. isolated in Spain from 2013 to 2015 produced a variety of carbapenemases including VIM-1, OXA-48, KPC-2, NDM-1 and VIM-2

Objectives: There is little information about carbapenemase-producing (CP) Citrobacter spp.We studied the molecular epidemiology and microbiological features of CP Citrobacter spp. isolates collected in Spain (2013-15). Methods: In total, 119 isolates suspected of being CP by the EUCAST screening cut-off values were analysed. Carbapenemases and ESBLs were characterized using PCR and sequencing. The genetic relationship among Citrobacter freundii isolates was studied by PFGE. Results: Of the 119 isolates, 63 (52.9%) produced carbapenemases, of which 37 (58.7%) produced VIM-1, 20 (31.7%) produced OXA-48, 12 (19%) produced KPC-2, 2 (3.2%) produced NDM-1 and 1 (1.6%) produced VIM- 2; 9 C. freundii isolates co-produced VIM-1 plus OXA-48. Fourteen isolates (22.2%) also carried ESBLs: 8 CTX-M-9 plus SHV-12, 2 CTX-M-9, 2 SHV-12 and 2 CTX-M-15. Fifty-seven isolates (90.5%) were C. freundii, 4 (6.3%) were Citrobacter koseri, 1 (1.6%) was Citrobacter amalonaticus and 1 (1.6%) was Citrobacter braakii. By EUCAST breakpoints, eight (12.7%) of the CP isolates were susceptible to the four carbapenems tested. In the 53 CP C. freundii analysed by PFGE, a total of 44 different band patterns were observed. Four PFGE clusters were identified: cluster 1 included eight isolates co-producing VIM-1 and OXA-48; blaVIM-1 was carried in a class 1 integron (intI-blaVIM-1 - aacA4-dfrB1-aadA1-catB2-qacE¿1/sul1) and blaOXA-48 was carried in a Tn1999.2 transposon. Conclusions: We observed the clonal and polyclonal spread of CP Citrobacter spp. across several Spanish geographical areas. Four species of Citrobacter spp. produced up to five carbapenemase types, including coproduction of VIM-1 plus OXA-48. Some CP Citrobacter spp. isolates were susceptible to the four carbapenems tested, a finding with potential clinical implications

Effect of the COVID-19 pandemic on surgery for indeterminate thyroid nodules (THYCOVID): a retrospective, international, multicentre, cross-sectional study

Background Since its outbreak in early 2020, the COVID-19 pandemic has diverted resources from non-urgent and elective procedures, leading to diagnosis and treatment delays, with an increased number of neoplasms at advanced stages worldwide. The aims of this study were to quantify the reduction in surgical activity for indeterminate thyroid nodules during the COVID-19 pandemic; and to evaluate whether delays in surgery led to an increased occurrence of aggressive tumours.Methods In this retrospective, international, cross-sectional study, centres were invited to participate in June 22, 2022; each centre joining the study was asked to provide data from medical records on all surgical thyroidectomies consecutively performed from Jan 1, 2019, to Dec 31, 2021. Patients with indeterminate thyroid nodules were divided into three groups according to when they underwent surgery: from Jan 1, 2019, to Feb 29, 2020 (global prepandemic phase), from March 1, 2020, to May 31, 2021 (pandemic escalation phase), and from June 1 to Dec 31, 2021 (pandemic decrease phase). The main outcomes were, for each phase, the number of surgeries for indeterminate thyroid nodules, and in patients with a postoperative diagnosis of thyroid cancers, the occurrence of tumours larger than 10 mm, extrathyroidal extension, lymph node metastases, vascular invasion, distant metastases, and tumours at high risk of structural disease recurrence. Univariate analysis was used to compare the probability of aggressive thyroid features between the first and third study phases. The study was registered on ClinicalTrials.gov, NCT05178186.Findings Data from 157 centres (n=49 countries) on 87 467 patients who underwent surgery for benign and malignant thyroid disease were collected, of whom 22 974 patients (18 052 [78 center dot 6%] female patients and 4922 [21 center dot 4%] male patients) received surgery for indeterminate thyroid nodules. We observed a significant reduction in surgery for indeterminate thyroid nodules during the pandemic escalation phase (median monthly surgeries per centre, 1 center dot 4 [IQR 0 center dot 6-3 center dot 4]) compared with the prepandemic phase (2 center dot 0 [0 center dot 9-3 center dot 7]; p<0 center dot 0001) and pandemic decrease phase (2 center dot 3 [1 center dot 0-5 center dot 0]; p<0 center dot 0001). Compared with the prepandemic phase, in the pandemic decrease phase we observed an increased occurrence of thyroid tumours larger than 10 mm (2554 [69 center dot 0%] of 3704 vs 1515 [71 center dot 5%] of 2119; OR 1 center dot 1 [95% CI 1 center dot 0-1 center dot 3]; p=0 center dot 042), lymph node metastases (343 [9 center dot 3%] vs 264 [12 center dot 5%]; OR 1 center dot 4 [1 center dot 2-1 center dot 7]; p=0 center dot 0001), and tumours at high risk of structural disease recurrence (203 [5 center dot 7%] of 3584 vs 155 [7 center dot 7%] of 2006; OR 1 center dot 4 [1 center dot 1-1 center dot 7]; p=0 center dot 0039).Interpretation Our study suggests that the reduction in surgical activity for indeterminate thyroid nodules during the COVID-19 pandemic period could have led to an increased occurrence of aggressive thyroid tumours. However, other compelling hypotheses, including increased selection of patients with aggressive malignancies during this period, should be considered. We suggest that surgery for indeterminate thyroid nodules should no longer be postponed even in future instances of pandemic escalation.Funding None.Copyright (c) 2023 Published by Elsevier Ltd. All rights reserved

On the sensitivity of the HAWC observatory to gamma-ray bursts

We present the sensitivity of HAWC to Gamma Ray Bursts (GRBs). HAWC is a very high-energy gamma-ray observatory currently under construction in Mexico at an altitude of 4100 m. It will observe atmospheric air showers via the water Cherenkov method. HAWC will consist of 300 large water tanks instrumented with 4 photomultipliers each. HAWC has two data acquisition (DAQ) systems. The main DAQ system reads out coincident signals in the tanks and reconstructs the direction and energy of individual atmospheric showers. The scaler DAQ counts the hits in each photomultiplier tube (PMT) in the detector and searches for a statistical excess over the noise of all PMTs. We show that HAWC has a realistic opportunity to observe the high-energy power law components of GRBs that extend at least up to 30 GeV, as it has been observed by Fermi LAT. The two DAQ systems have an energy threshold that is low enough to observe events similar to GRB 090510 and GRB 090902b with the characteristics observed by Fermi LAT. HAWC will provide information about the high-energy spectra of GRBs which in turn could help to understanding about e-pair attenuation in GRB jets, extragalactic background light absorption, as well as establishing the highest energy to which GRBs accelerate particles

Revisión. Ventajas y desventajas de los sistemas de control climático aplicados en agricultura de precisión

Today agriculture is changing in response to the requirements of modern society, where ensuring food supply through practices such as water conservation, reduction of agrochemicals and the required planted surface, which guarantees high quality crops are in demand. Greenhouses have proven to be a reliable solution to achieve these goals; however, a greenhouse as a means for protected agriculture has the potential to lead to serious problems. The most of these are related to the inside greenhouse climate conditions where controlling the temperature and relative humidity (RH) are the main objectives of engineering. Achieving appropriate climate conditions to ensure high yield and quality crops reducing energy consumption have been the objective of investigations for some time. Different schemes in control theories have been applied in this field to solve the aforementioned problems. Therefore, the objective of this paper is to present a review of different control techniques applied in protected agriculture to manage greenhouse climate conditions, presenting advantages and disadvantages of developed control platforms in order to suggest a design methodology according to results obtained from different investigations.Hoy en día la agricultura está cambiando de acuerdo a las necesidades de la nueva sociedad. Las nuevas tendencias son asegurar la producción de alimentos a través de prácticas tales como ahorro de agua, reducción en el uso de agroquímicos y el espacio requerido para sembrar los cultivos mientras se garantiza la alta calidad de los cultivos. Los invernaderos han demostrado ser una solución viable para garantizar estos objetivos. Sin embargo, el uso de un invernadero conlleva serios problemas. Los más importantes están relacionados con las condiciones del microclima dentro del invernadero, donde el objetivo de la ingeniería es controlar la temperatura y humedad relativa (RH). Alcanzar las condiciones adecuadas del microclima para garantizar la alta productividad y calidad de los cultivos mientras se reducen los consumos de energía ha sido el objetivo de diversos investigadores a través del tiempo. Diversos esquemas de teoría de control han sido aplicados con el objetivo de resolver los problemas antes mencionados. Por lo tanto, el objetivo de este artículo es presentar una revisión de las diferentes técnicas de control aplicadas en agricultura de precisión para manejar las condiciones del microclima del invernadero, presentando las ventajas y desventajas de los sistemas desarrollados con la finalidad de proponer una metodología de diseño de acuerdo a los resultados obtenidos de las diferentes investigaciones

Dengue virus in Mexican bats

Individuals belonging to five families, 12 genera, and 19 different species of bats from dengue endemic areas in the Gulf and Pacific coasts of Mexico were examined by ELISA, RT–PCR, and for the presence of dengue virus (DV) NS1 protein. Nine individuals from four species were seropositive by ELISA: three insectivorous, Myotis nigricans (four positives/12 examined), Pteronotus parnellii (3/19), and Natalus stramineus (1/4), and one frugivorous Artibeus jamaicensis (1/35) (12·86% seroprevalence in positive species). DV serotype 2 was detected by RT–PCR in four samples from three species (all from the Gulf coast – rainy season): two frugivorous, A. jamaicensis (2/9), and Carollia brevicauda (1/2), and one insectivorous, M. nigricans (1/11). The latter was simultaneously positive for NS1 protein. DV RT–PCR positive animals were all antibody seronegative. M. nigricans showed positive individuals for all three tests. This is the first evidence suggesting the presence of DV in bats from Mexico

Review. Advantages and disadvantages of control theories applied in greenhouse climate control systems

Today agriculture is changing in response to the requirements of modern society, where ensuring food supply through practices such as water conservation, reduction of agrochemicals and the required planted surface, which guarantees high quality crops are in demand. Greenhouses have proven to be a reliable solution to achieve these goals; however, a greenhouse as a means for protected agriculture has the potential to lead to serious problems. The most of these are related to the inside greenhouse climate conditions where controlling the temperature and relative humidity (RH) are the main objectives of engineering. Achieving appropriate climate conditions to ensure high yield and quality crops reducing energy consumption have been the objective of investigations for some time. Different schemes in control theories have been applied in this field to solve the aforementioned problems. Therefore, the objective of this paper is to present a review of different control techniques applied in protected agriculture to manage greenhouse climate conditions, presenting advantages and disadvantages of developed control platforms in order to suggest a design methodology according to results obtained from different investigations

Quadratic Non-Linear Optical Properties of the poly(2,5-bis(but-2-ynyloxy) Benzoate Containing the 2-(ethyl(4-((4-nitrophenyl)buta-1,3-diynyl)phenyl)amino)ethanol) Chromophore

Excellent quadratic non-linear optical (ONL-2) properties of the poly(2,5-bis(but-2-ynyloxy) benzoate, containing a polar diacetylene as a chromophore, were found. According with the Maker fringes method, oriented polymer films showing an order parameter of ∼0.23 can display outstanding and stable Second Harmonic Generation (SHG) effects under off-resonant conditions (SHG-532 nm). Also, the macroscopic non-linear optical (NLO)-coefficients were evaluated under the rod-like molecular approximation, obtaining: χzzz(2) and χzxx(2) in the order of 280 ± 10 and 100 ± 10 pm V−1, respectively. The mechanical and chemical properties, in addition to the large ONL-2 coefficients exhibited by this polymer, make it a promising organic material in the development of optoelectronic/photonic devices

Agentes asociados y su papel en la declinación y muerte de encinos (quercus, fagaceae) en el centro-oeste de México

ABSTRACT This study was carried out to identify biotic and abiotic agents and to determine their role in oak decline and death in five states of the Mexican Republic: Aguascalientes, Colima, Guanajuato, Jalisco and Nayarit. Two 0.1-ha permanent plots were established at the most representative forested areas with tree death, dieback and bleeding cankers in these states and sampled during 2003, 2004 and 2005 (10 plots in total). Field observations were made, and foliage, branch, trunk and soil samples were collected to identify the associated agents in each plot. Isolates from bleeding cankers were made in situ. The most commonly found biotic agents were: Phytophthora cinnamomi, Pythium sp., Hypoxylon antropunctatum, Ganoderma spp., Armillaria sp., Apiognomonia quercina, the dwarf mistletoe (Phoradendron villosum), bark borers, insect defoliators and livestock overgrazing. Among the abiotic stress factors commonly found were the effects due to low temperature-lack of water and fire. Based on their incidence, P. cinnamomi, H. antropunctatum and low temperature-lack of water were considered the most important contributors to the decline and death of the oaks. P. cinnamomi was identified in four of the 10 plots, mainly in Colima and Jalisco; H. antropunctatum was detected in six plots, particularly in Nayarit and Aguascalientes. Stem cankers (cracks) due to low temperature-lack of water were observed in four plots, damaging 52% of the trees in Aguascalientes and 24% in Guanajuato. It was concluded that P. cinnamomi is killing some oak species, H. antropunctatum is colonizing weakened trees, and low temperature-lack of water is causing stress and in some cases death in oaks.RESUMEN Este estudio tuvo como objetivo identificar y determinar el papel tanto de los factores bióticos como de los abióticos asociados a la declinación y muerte del encino en cinco estados de la República mexicana: Aguascalientes, Colima, Guanajuato, Jalisco y Nayarit. Para esto, se establecieron dos sitios permanentes de 0.1 ha en cada una de las áreas más representativas (con arbolado muerto, con muerte regresiva y cancros con exudado) de los estados mencionados (10 sitios en total), los cuales fueron muestreados durante 2003, 2004 y 2005. Se hicieron observaciones y colectas en campo de follaje, ramas, tronco y suelo, para identificar a los diferentes agentes de daño en cada sitio. Se realizaron aislamientos in situ de cancros con exudado en medios específicos. Los agentes bióticos más comunes fueron: Phytophthora cinnamomi, Pythium sp., Hypoxylon antropunctatum, Ganoderma sp., Armillaria sp., Apiognomonia quercina, el muérdago enano (Phoradendron villosum), barrenadores, defoliadores y sobrepastoreo. Entre los abióticos causantes de estrés y muerte, destacaron los daños ocasionados por las bajas temperaturas-falta de agua y fuego. Con base en su incidencia, P. cinnamomi, H. antropunctatum y las bajas temperaturas-falta de agua son considerados los factores de mayor importancia en la declinación y muerte del encino. P. cinnamomi fue identificado en cuatro de los diez sitios, particularmente de Colima y Jalisco; H. antropunctatum fué detectado en seis de diez, principalmente en los rodales de Nayarit y Aguascalientes. Los cancros de troncos (rajaduras), debido a las bajas temperaturas-falta de agua, se observaron en cuatro sitios, afectando al 52% de los árboles en Aguascalientes y 24% en Guanajuato. Se concluye que P. cinnamomi está ocasionando la muerte de algunas especies de encino, H. antropunctatum está coloni-zando árboles debilitados y las bajas temperaturas-falta de agua están participando como agentes causantes de estrés y muerte en algunos casos

Interaction of Metallothionein-2 with Platinum-Modified 5′-Guanosine Monophosphate and DNA

Human metallothioneins (MTs), a family of cysteine- and metal-rich metalloproteins, play an important role in the acquired resistance to platinum drugs. MTs occur in the cytosol and the nucleus of the cells and sequester platinum drugs through interaction with their zinc–thiolate clusters. Herein, we investigate the ability of human Zn7MT-2 to form DNA–Pt–MT cross-links using the cisplatin- and the transplatinmodified plasmid DNA pSP73. Immunochemical analysis of MT-2 showed that the monofunctional platinum–DNA adducts formed DNA–cis/trans-Pt–MT cross-links and that platinated MT-2 was released from the DNA–trans-Pt–MT cross-links with time. The DNA–cis-/trans-Pt–MT cross-links were also formed in the presence of 2 mM glutathione, a strong S-donor ligand. Independently, we used 5′-guanosine monophosphate (5′-GMP) platinated at N7 position as a model of monofunctional platinum-DNA adducts. Comparison of reaction kinetics revealed that the formation of ternary complexes between Zn7MT-2 and cis-Pt–GMP was faster than that of the trans isomer. The analysis of the reaction products with time showed that while the formation of ternary GMP–trans-Pt–MT complex(es) is accompanied by 5'-GMP release, a stable ternary GMP–cis-Pt–MT complex is formed. In the latter complex, a fast initial formation of two Pt–S bonds was followed by a slow formation of an additional Pt–S bond yielding an unusual Pt(II)S3N coordination with N7-GMP as the only N-donor ligand. The ejection of negligible zinc from the zinc–thiolate clusters implies the initial formation of Zn–(μ-SCys)–Pt bridges involving the terminal thiolate ligands. The biological implications of these studies are discussed