Partial sequencing of the bottle gourd genome reveals markers useful for phylogenetic analysis and breeding

<p>Abstract</p> <p>Background</p> <p>Bottle gourd [<it>Lagenaria siceraria </it>(Mol.) Standl.] is an important cucurbit crop worldwide. Archaeological research indicates that bottle gourd was domesticated more than 10,000 years ago, making it one of the earliest plants cultivated by man. In spite of its widespread importance and long history of cultivation almost nothing has been known about the genome of this species thus far.</p> <p>Results</p> <p>We report here the partial sequencing of bottle gourd genome using the 454 GS-FLX Titanium sequencing platform. A total of 150,253 sequence reads, which were assembled into 3,994 contigs and 82,522 singletons were generated. The total length of the non-redundant singletons/assemblies is 32 Mb, theoretically covering ~ 10% of the bottle gourd genome. Functional annotation of the sequences revealed a broad range of functional types, covering all the three top-level ontologies. Comparison of the gene sequences between bottle gourd and the model cucurbit cucumber (<it>Cucumis sativus</it>) revealed a 90% sequence similarity on average. Using the sequence information, 4395 microsatellite-containing sequences were identified and 400 SSR markers were developed, of which 94% amplified bands of anticipated sizes. Transferability of these markers to four other cucurbit species showed obvious decline with increasing phylogenetic distance. From analyzing polymorphisms of a subset of 14 SSR markers assayed on 44 representative China bottle gourd varieties/landraces, a principal coordinates (PCo) analysis output and a UPGMA-based dendrogram were constructed. Bottle gourd accessions tended to group by fruit shape rather than geographic origin, although in certain subclades the lines from the same or close origin did tend to cluster.</p> <p>Conclusions</p> <p>This work provides an initial basis for genome characterization, gene isolation and comparative genomics analysis in bottle gourd. The SSR markers developed would facilitate marker assisted breeding schemes for efficient introduction of desired traits.</p

A design reuse technology to increase productivity through automated corporate memory system

Increasing competitiveness in engineering industry pressurizes companies to improve productivity of every single element in processes. As a result of this pressure, increasing productivity of design staff and design systems is systematically investigated by industry professionals and researchers. Design reuse appears as an effective approach to increase productivity levels. It has been already used personally by designers consciously or subconsciously. This paper proposes a computer-based design reuse methodology developed with the purpose of efficient corporate memory usage. The paper focused on steel construction infrastructure design process; however, its approach can be easily implemented in different engineering design processes. 2016, The Natural Computing Applications Forum.Scopu

Correlation of PET-CT with bone marrow biopsy to evaluate bone marrow infiltration in HL case series

10th Eurasian Hematology Oncology Congress -- OCT 08-11, 2019 -- Istanbul, TURKEYWOS: 000489290100100

Collection and morphological characterization of Lagenaria siceraria germplasm from the Mediterranean region of Turkey

The landraces of Lagenaria siceraria in Turkey show great diversity for morphologic traits, particularly in fruit size and shape even though Turkey is not centre of genetic diversity for L. siceraria. In Turkey, L. siceraria is used as food, musical instruments and containers, according to the type and shape of their fruits. Its diversity has been gradually declining over the last 25 years. With the aim of assessing variations in plant, fruit and seed morphology among the L. siceraria landraces, 15 field trips for collection of germplasm to southern parts of Turkey (Mediterranean region) were carried out in the period of 2003-2004. The study also aimed at developing a representative core collection of the material to guide future studies and uses concerning its existing genetic diversity in Turkey. A total of 182 accessions (fruits and/or seeds) were collected. The seeds from all the sample material were sown in green house conditions at the experimental station of the Faculty of Agriculture of the Mustafa Kemal University in Hatay (Antakya), (36A degrees 18' 22'' N, 36A degrees 13' 33'' E, 82 m). In the spring of 2004, 162 out of the 182 sown seeds germinated and developed into viable seedlings. These were further transplanted in the field in 10 replicates. The morpho-agronomic characterization was carried out following the international standards for crop descriptors set by Bioversity International. The data were subject to both descriptive statistics and multivariate analysis by Principle Component Analysis. The descriptive statistics revealed that the whole collection exhibits a great deal of morphological diversity and that the subset core collection represents most of the variability. The first three principle components, calculated by using six quantitative traits, explained 26%, 21% and 17% (a total of 64%) of the total variation. Among the studied accessions, no apparently distinct patterns such as geographical origin were detected. This may suggest that the accessions have been introduced to Turkey from multiple locations and/or their diversity had been distributed almost evenly across the Mediterranean region of this country. Based on our results from the morphological characterization, 30 genotypes were selected to develop a subgroup (core) collection in order to represent most of the genetic diversity of all accessions. The work presented here is the first report about morphological characterization of Turkish L. siceraria germplasm