Regresión tumoral espontánea. A propósito de dos casos

Two cases of spontaneous tumor regression (STR) occurring in a patient with non Hodgkin lymphoma and in another patient with squamous carcinoma of the lung are presented. Both cases fulfill the criteria of STR defined by Everson and Cole. Recent results obtained in basic and clinical studies have indicated that immunological mechanisms could play an important role in STR. The mediator effects more frequently referred are: 1) generation of antineoplastic cytotoxic cells; 2) production of immunoregulatory cytokines by lymphocytes and monocytes, and 3) possible cross reaction between tumor and bacterial antigens. These mechanisms of action are discussed in relation to the presented case

A MIQE-Compliant Real-Time PCR Assay for Aspergillus Detection

PMCID: PMC3393739This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

Multiple cycles of dose-intensive chemotherapy with repeated stem cell support as induction treatment in metastatic breast cancer: a feasibility study

The purpose of this trial was to study feasibility and tolerance of a dose-intensive multicyclic alternating induction chemotherapy with repeated stem cell support in a series of 43 metastatic breast cancer patients. Anthracycline-naive patients (n = 21) received cyclophosphamide 2.5 g/m2 plus doxorubicin 80 mg/m2 alternating every 14 days with paclitaxel 200-350 mg/m2 plus cisplatin 120 mg/m2. Patients who had previously received anthracyclines (n = 22) received cisplatin 120 mg/m2 plus etoposide 600 mg/m2 alternating with paclitaxel 200-350 mg/m2 plus ifosfamide 8 g/m2. Peripheral blood stem cells were infused after every course except the first, with a median CD34+ dose of 2.1 ´ 106/kg per cycle. Positive selection of CD34+ cells was performed in good mobilizers. The median number of cycles administered was six (4-8), and the time interval between them was 17 days. Median summation dose intensities (SDI) actually administered for the CA-TP and PE-TI protocol were 4.95 and 4.69, respectively (87% of scheduled SDI). There were 15 complete (35%) and 21 partial responses (49%), for an overall response rate of 84% (95% CI, 73%-95%). Infection or neutropenic fever occurred in 50% of the cycles. There was one treatment-related death. After a median follow-up of 26 months, the median event-free-survival was 12 months (95% CI: 10-14) and overall survival was 31 months. These high dose-intensity induction treatments seem to be feasible with sequential stem cell support

Los bebés prematuros y su desarrollo visual

Las autoras señalan la incidencia de la prematuridad en el desarrollo visual del recién nacido y la importancia de la detección precoz de las alteraciones visuales. Describen la intervención del Equipo de Atención Temprana del Centro de Recursos Educativos de la ONCE en Barcelona, en el marco del acuerdo suscrito entre esta entidad y cuatro hospitales barceloneses de referencia para la detección precoz en unidades de Cuidados Intensivos de Neonatología. Es fundamental que el niño sea valorado inmediatamente por un equipo multidisciplinar, y que este asesore a los padres para asegurar una adecuada intervención parental, basada en el conocimiento de la importancia de los vínculos afectivos. El Equipo de Atención Temprana de la ONCE lleva a cabo la valoración de la visión funcional del niño, que, una vez recibida el alta hospitalaria, es derivado al Centro de Recursos Educativos, donde se efectuará el seguimiento con arreglo al plan de intervención individualizado, de acuerdo con la situación del bebé y de su familia

Una experiencia de trabajo en grupo desde la atención temprana

Las autoras de este artículo, integrantes del Equipo de Atención Temprana del Centro de Recursos Educativos de la ONCE en Barcelona, resaltan la importancia del trabajo en grupo dentro del proceso asistencial, y presentan su experiencia con un grupo de madres y niños con discapacidad visual y deficiencias múltiples. Describen la metodología adoptada, así como la evolución de la experiencia y los objetivos alcanzados

Validating scanned foot images and designing customized insoles on the cloud

© 2016 IEEE.People with foot problems need special healthcare: foot care. Customized insoles can provide this care. They are inserts that are placed in the shoes. They correct biomechanical and postural inaccuracies in foot. Insole production contains four phases: foot image scanning, image validation, insole design and insole manufacturing. Currently, image scanning and validation is separated in location and time, i.e. podiatrists take images and insole designers validate them at different location and at different time. A cloud-based solution, the CloudSME one-stop shop simulation platform, enables remote access to image validation and insole design service deployed and running on the Cloud. The remote access allows podiatrists validating scanned image while the patient is in their offices. The simulation platform also supports remote design of customized insoles

Single instrument for hemostatic control in laparoscopic partial nephrectomy in a porcine model without renal vascular clamping

This is a copy of an article published in the Journal of Endourology ©2011 copyright Mary Ann Liebert, Inc.; Journal of Endourology is available online at: http://www.liebertpub.com/end[EN] Materials and Methods: We performed a comparative experimental study between a new radiofrequency (RF)-assisted device consisting of a handheld instrument that simultaneously conducts coagulation and cutting tasks without hilar clamping vs a standard technique with hilar clamping. A porcine model was used (10 animals per group) with survival of 17 days. Results: The estimated blood loss with the new device was significantly lower than with the standard technique (15.5 +/- 23.7 vs 79.4 +/- 76.3 mL). Although transection time was longer with the new device (10.7 +/- 13.7 vs 2.1 +/- 1.2 min), the total operative time was significantly shorter (35.3 +/- 13.7 vs 60.2 +/- 10.5 min). Evidence of localized urinary extravasation (urinoma) was identical in both groups (five cases). The group subjected to the new device, however, showed a significantly higher number of cases of leakage after conducting the methylene-blue test: eight (80%) cases vs only one (11%) with the standard technique. Necrosis depth was significantly greater with the new device (6.6 +/- 0.9 vs <1 mm). Conclusions: The experimental results suggest that the proposed RF-assisted device provides adequate hemostatic control during transection of the renal parenchyma without additional instruments or surgical maneuvers and could therefore be a valuable adjunct for LPN without vascular clamping. The device was unsuccessful in effectively sealing the collecting system.This work received financial support from the Spanish "Plan Nacional de I + D + I del Ministerio de Ciencia e Innovacion," Grant No. TEC2008-01369/TEC. The translation of this paper was funded by the Universidad Politecnica de Valencia, Valencia, Spain.Subira Rios, J.; Sanchez Zalabardo, JM.; Burdio, F.; Berjano, E.; Moros, M.; Gonzalez, A.; Navarro, A.... (2011). Single instrument for hemostatic control in laparoscopic partial nephrectomy in a porcine model without renal vascular clamping. Journal of Endourology and Part B, Videourology. 25(6):1005-1011. https://doi.org/10.1089/end.2010.0557S1005101125

Specific patterns of brain alterations underlie distinct clinical profiles in Huntington's disease

Huntington's disease (HD) is a genetic neurodegenerative disease which involves a triad of motor, cognitive and psychiatric disturbances. However, there is great variability in the prominence of each type of symptom across individuals. The neurobiological basis of such variability remains poorly understood but would be crucial for better tailored treatments. Multivariate multimodal neuroimaging approaches have been successful in disentangling these profiles in other disorders. Thus we applied for the first time such approach to HD. We studied the relationship between HD symptom domains and multimodal measures sensitive to grey and white matter structural alterations. Forty-three HD gene carriers (23 manifest and 20 premanifest individuals) were scanned and underwent behavioural assessments evaluating motor, cognitive and psychiatric domains. We conducted a multimodal analysis integrating different structural neuroimaging modalities measuring grey matter volume, cortical thickness and white matter diffusion indices - fractional anisotropy and radial diffusivity. All neuroimaging measures were entered into a linked independent component analysis in order to obtain multimodal components reflecting common inter-subject variation across imaging modalities. The relationship between multimodal neuroimaging independent components and behavioural measures was analysed using multiple linear regression. We found that cognitive and motor symptoms shared a common neurobiological basis, whereas the psychiatric domain presented a differentiated neural signature. Behavioural measures of different symptom domains correlated with different neuroimaging components, both the brain regions involved and the neuroimaging modalities most prominently associated with each type of symptom showing differences. More severe cognitive and motor signs together were associated with a multimodal component consisting in a pattern of reduced grey matter, cortical thickness and white matter integrity in cognitive and motor related networks. In contrast, depressive symptoms were associated with a component mainly characterised by reduced cortical thickness pattern in limbic and paralimbic regions. In conclusion, using a multivariate multimodal approach we were able to disentangle the neurobiological substrates of two distinct symptom profiles in HD: one characterised by cognitive and motor features dissociated from a psychiatric profile. These results open a new view on a disease classically considered as a uniform entity and initiates a new avenue for further research considering these qualitative individual differences

La nécropole néolithique de la Feixa del Moro (Juberri, Andorre): Examen et nouvelles données

At the beginning of the 1980s, a series of archaeological interventions carried out by what was previously called the 'Servei d'Investigacions Arqueológiques del Patrimoni Artistic Nacional d'Andorra' in a Pyrenean valley in Andorra allowed the investigation of the Feixa del Moro site. In a high-altitude area below a series of abandoned terraces, several dwellings and burial structures were located, all of them with chronologies ranging between the Early and the Middle Neolithic (from the mid 5th millennium to the early 4th millennium cal. BC). The distinctiveness of this site does not only lie in its geographical location, nor in the kind of structures discovered, but also in the very good state of preservation of the human bone material recovered from the burials, making Feixa del Moro one of the reference sites for the Neolithic in the Pyrenees and, in general, the Western Mediterranean. So far, sites with a similar conservation of both bones and burial structures are really uncommon. Moreover, the concentration in so small an area, and in the same stratigraphic unit, of such a diversity of evidence, including burials, silos and hearths, is yet more unusual. There are no similar sites in Andorra, or even in the entire Pyrenees. The only other burial site of comparable chronology discovered in the area is the Segudet site, and only a few high-altitude Neolithic dwelling sites are known. Even if cist burials are quite common in the northeast of the Iberian Peninsula and in Southern France, Feixa del Moro is the first that has been found at high altitude. The archaeological work undertaken between 1983 and 1985 provided a picture of a farming community belonging to the so-called 'Sepulcros de fosa' Culture, established in the very heart of the Pyrenees and, thus, highlighted the complexity of Neolithic settlement patterns, even in mountainous zones. At the same time, several analyses of the archaeological materials were already carried out, making Feixa del Moro a reference site for archaeological research even now. Nevertheless, three decades later, new methodologies and the technical advances available are allowing archaeologists to refine old interpretations, to reopen old debates and to carry out new analyses that can improve our understanding of the past. In this respect, since 2011, within the research project 'Aproximación a las primeras comunidades neolíticas del NE peninsular a través de sus prácticas funerarias' (HAR2011-23149), funded by the Spanish Ministry for the Economy and Competitiveness, a group of interdisciplinary researchers have begun to study several Neolithic burial contexts in the northeast of the Iberian Peninsula, among which Feixa del Moro. Following this perspective, in this paper, we present the outcome of the new analyses carried out on the burial goods and of the biochemistry and radiocarbon analyses carried out on the human bone material from the three cist burials of Feixa del Moro, with the aim of better understanding the early farming communities who settled in the Pyrenees. Since the last archaeological work carried out in the 1990s, large quantities of data have been lost. This has produced a certain degree of confusion and misunderstanding that has been repeated in other studies undertaken a posteriori on the site by other scholars. Some of these interpretations need to be revised. That it is why, within the current research project, we are not only bringing in new analyses, but also re-examining all the old written and graphic information available, as well as the state of the conserved archaeological material. The data presented in this paper resume all the available information on the Feixa del Moro site, correcting old mistakes and bias, updating the 1980s archaeological registers and presenting new analyses as well. Our aim is to ensure that Feixa del Moro remains a reference site for the Pyrenean and Western Mediterranean Neolithic. At the same time, we wish to encourage other researchers to undertake new analyses and to embrace new perspectives in order to improve our understanding of Neolithic societies

Role of flow cytometry immunophenotyping in the diagnosis of leptomeningeal carcinomatosis

PURPOSE: To explore the contribution of flow cytometry immunophenotyping (FCI) in detecting leptomeningeal disease in patients with solid tumors. EXPERIMENTAL DESIGN: Cerebrospinal fluid (CSF) samples from 78 patients who received a diagnosis of epithelial-cell solid tumors and had clinical data suggestive of leptomeningeal carcinomatosis (LC) were studied. A novel FCI protocol was used to identify cells expressing the epithelial cell antigen EpCAM and their DNA content. Accompanying inflammatory cells were also described. FCI results (positive or negative for malignancy) were compared with those from CSF cytology and with the diagnosis established by the clinicians: patients with LC (n = 49), without LC (n = 26), and undetermined (n = 3). RESULTS: FCI described a wide range of EpCAM-positive cells with a hyperdiploid DNA content in the CSF of patients with LC. Compared with cytology, FCI showed higher sensitivity (75.5 vs 65.3) and negative predictive value (67.6 vs 60.5), and similar specificity (96.1 vs 100) and positive predictive value (97.4 vs 100). Concordance between cytology and FCI was high (Kp = 0.83), although misdiagnosis of LC did not show differences between evaluating the CSF with 1 or 2 techniques (P = .06). Receiver-operator characteristic curve analyses showed that lymphocytes and monocytes had a different distribution between patients with and without LC. CONCLUSION: FCI seems to be a promising new tool for improving the diagnostic examination of patients with suspicion of LC. Detection of epithelial cells with a higher DNA content is highly specific of LC, but evaluation of the nonepithelial cell compartment of the CSF might also be useful for supporting this diagnosis