Automated Teller Machine (ATM)- a Pathogen City a Surveillance Report From Locations in and Around Madurai City, Tamil Nadu, India

ATM is used by millions of people in a day. It is meant to be a public utility device. Hence the microorganisms plays a major role in accommodating the safer place, ATM. Hence to this account an elaborate survey was taken for complete assessment of microbiology in and around Madurai city. Swabs were collected from each ATM screen, buttons, floor, users hand, exposure of plates and also extended the work in relation with microorganisms prevalent in ladies toilet the samples collected from ATM were plated in nutrient agar plates. The results showed the presence of increased bacterial count subsequently, most pathogens on characterization extended revealed the genus of the particular organism E-coli, Pseudomonas, Staphylococcus aures, Klebsiella, Micrococcus, Salmonella, Serratia and fungal species included Aspergillus sp, Mucor sp and Fusarium. Antibiogram study of bacteria also provides us information about the antibiotic resistance pattern of the bacterial isolates

Automated Teller Machine (ATM)- A “Pathogen City” – A surveillance Report from Locations in and around Madurai City, Tamil Nadu, India

ATM is used by millions of people in a day. It is meant to be a public utility device.Hence the microorganism’s plays a major role in accommodating the safer place, ATM.Hence to this account an elaborate survey was taken for complete assessment of microbiology in and around Madurai city. Swabs were collected from each ATM screen, buttons, floor, user’s hand, exposure of plates and also extended the work in relation with microorganisms prevalent in ladies toilet the samples collected from ATM were plated in nutrient agar plates. The results showed the presence of increased bacterial count subsequently, most pathogens on characterization extended revealed the genus of the particular organism E-coli, Pseudomonas, Staphylococcus aures, Klebsiella, Micrococcus, Salmonella, Serratia and fungal species included Aspergillus sp, Mucor sp and Fusarium. Antibiogram study of bacteria also provides us information about the antibiotic resistance pattern of the bacterial isolates.

Bio prospecting of Riboflavin producing bacteria from different riboflavin enriched food sources

Riboflavin is an essential, water-soluble vitamin (B2) and a component of basic cellular metabolism. The aim of the present study is to isolate and characterize riboflavin producing bacteria from different food sources. Ten different riboflavin enriched food sources were collected from Vellore district. Totally 72 bacterial strains were isolated and cultured on nutrient agar plates. Out of these, 43 strains were identified as riboflavin producers. Isolated bacterial strains HDS27, HDS07, HDS14, HDS18, HDS38 and HDS54 isolated from milk, mushroom, spinach, lamb kidney, beef liver and mackerel fish were found to be potent riboflavin producers. Based on morphological, biochemical and molecular characterization, the potent strains were identified as Lactobacillus plantarum (HDS27), Bacillus cereus (HDS07), Delftia tsuruhatensis (HDS14), Citrobacter freundii (HDS18), Enterobacter cloacae (HDS38) and Bacillus cereus (HDS54). The selected potent isolates HDS27 from milk and HDS07 from mushroom showed a maximum riboflavin production of 3.69 mg/L and 2.9mg/L respectively. The present study explores the riboflavin producing novel bacteria from different food sources. This is the first report that the Enterobacter cloacae isolated from beef liver, Delftia tsuruhatensis from spinach and Citrobacter freundii from lamb kidney has the ability to produce riboflavin. These potent strains could be a better starter for substituting the conventional bacteria for large scale production of riboflavin in industry

Traditional Zardosi Embroidery in the Design and Development of Custom Wall Decor

oai:ojs2.glim.vyomhansjournals.com:article/2Background: Zardozi embroidery is a traditional Indian method of surface decoration and needlework. This ancient embroidery uses metal wires, thread, beads, sequins, and stones. Once used to adorn royal attire with gold wires, zardozi now employs more affordable metals. It is commonly found in sarees, dupattas, and lehengas, with a significant market in wedding attire for its regal appeal. Purpose: Every year, Zardozi’s local and foreign markets grow. The government provides programs and incentives to support Zardozi craftsmen. However, Zardozi items are expensive, reserved for special occasions, and not affordable for all. As a result, machine-made imitations have emerged. Modern machinery affects traditional artisans, prompting new research to reduce costs and improve theirquality of life.Methods: Zardosi\u27s works are done on clothing to enhance its appearance. However, there is a need to make the effort to teach the general public these procedures and make the Aari stitched sample available to everyone. Therefore, we must create a product that is both economical and accessible to everyone. A few research studies on motif selection, frame size, and product pricing were examined, and the items were prepared. Wall-hanging production has been scheduled.Results: A fresh attempt at creating the zardozi wall hanging was created using the appropriate fabric, and its appearance, cost, and client acceptability will all be favorable. In contrast to the clothing, the item was reasonably priced and looked good hanging on the wall. Therefore, the majority of clients might be drawn to it. Conclusions: Craft training was undergone by the artisan, and by adopting the learned experience, a new product of embroidered wall hanging was prepared successfully.  Keywords: Zardosi Embroidery, Wall Decor, Zardhosi craftsmen, Zardosi wall hangin

Conversion of Citrus Fruit Peel into a Value-Added Product, Bio-Oil

The present study aimed to investigate the bio-oil from the blended citrus fruit peel by hydrothermal liquefaction process. Huge amounts of fruit peel waste are disposed of in the open environment without any proper management. Such fruit peels are considered a potential bio-resource to be converted into economically valuable products like bio-oil. Since the citrus fruit peel is a rich source of moisture content, a hydrothermal liquefaction process was introduced to produce bio-oil from cellulose, and lignocellulose. The experimental design against temperature, time, and biomass concentration optimization was carried out which was confirmed by the ANOVA f and p test that reveals time and temperature influenced the bio-oil yield drastically. As the time and temperature rise more than 60 min and 280°C, the volatile substance present in the biomass converts itself into solid residue which has a negative impact on bio-oil production, compared with biomass concentration. The maximum yield of bio-oil was recorded as 29.4% at 280°C at 60min reaction time and 80g/200mL concentration as optimized parameters. The GCMS reveals the presence of hydrocarbons and alkanethiol which are flammable and hold the standards of commercial transportation fuel but hold nitrogen and oxygen-containing compounds to pull down the fuel standards. Thus, the produced bio-oil can be blended with the transportation fuel after the upgradation process for efficient results

A High Gain DC-DC Converter with Grey Wolf Optimizer Based MPPT Algorithm for PV Fed BLDC Motor Drive

Photovoltaic (PV) water pumping systems are becoming popular these days. In PV water pumping, the role of the converter is most important, especially in the renewable energy-based PV systems case. This study focuses on one such application. In this proposed work, direct current (DC) based intermediate DC-DC power converter, i.e., a modified LUO (M-LUO) converter is used to extricate the availability of power in the high range from the PV array. The M-LUO converter is controlled efficiently by utilizing the Grey Wolf Optimizer (GWO)-based maximum power point tracking algorithm, which aids the smooth starting of a brushless DC (BLDC) motor. The voltage source inverter’s (VSI) fundamental switching frequency is achieved in the BLDC motor by electronic commutation. Hence, the occurrence of VSI losses due to a high switching frequency is eliminated. The GWO optimized algorithm is compared with the perturb and observe (P&O) and fuzzy logic based maximum power point tracking (MPPT) algorithms. However, by sensing the position of the rotor and comparing the reference speed with the actual speed, the speed of the BLDC motor is controlled by the proportional-integral (PI) controller. The recent advancement in motor drives based on distributed sources generates more demand for highly efficient permanent magnet (PM) motor drives, and this was the beginning of interest in BLDC motors. Thus, in this paper, the design of a high-gain boost converter optimized by a GWO algorithm is proposed to drive the BLDC-based pumping motor. The proposed work is simulated in MATLAB-SIMULINK, and the experimental results are verified using the dsPIC30F2010 controller

No difference in clinical outcomes for African American and White patients hospitalized with SARS-CoV-2 pneumonia in Louisville, Kentucky

Introduction: Current literature indicates that African American individuals are at increased risk of becoming infected with the SARS-CoV-2 virus and suffer higher SARS-CoV-2-related mortality rates. However, there is a lack of consensus as to how the clinical outcomes of African American patients differ from those of other groups. The objective of this study was to define the clinical outcomes of African American and White hospitalized patients with SARS-CoV-2 community-acquired pneumonia (CAP) in Louisville, Kentucky. Methods: This was a retrospective cohort study of hospitalized patients with SARS-CoV-2 CAP at eight hospitals in Louisville, Kentucky. Severity of CAP at time of hospitalization was evaluated using the pneumonia severity index (PSI), CURB-65 score and SARS-CoV-2 viral load. The following thirteen clinical outcomes were compared: discharge alive to home, time to home discharge, admission to the ICU, length of ICU stay, need for invasive mechanical ventilation (IMV), duration of IMV, development of acute respiratory distress syndrome (ARDS), development of septic shock, need for vasopressors, development of cardiovascular events, time to cardiovascular events, in-hospital mortality, and time to death. Results: A total of 541 patients were eligible for this study, 343 White (63%) and 198 African American (37%). None of the thirteen clinical outcomes were statistically significantly different between the two groups. Conclusions: This study indicates that African American and White patients do not have different clinical outcomes after the point of hospitalization due to SARS-CoV-2 CAP

Sphingomyelin Synthases Regulate Protein Trafficking and Secretion

Sphingomyelin synthases (SMS1 and 2) represent a class of enzymes that transfer a phosphocholine moiety from phosphatidylcholine onto ceramide thus producing sphingomyelin and diacylglycerol (DAG). SMS1 localizes at the Golgi while SMS2 localizes both at the Golgi and the plasma membrane. Previous studies from our laboratory showed that modulation of SMS1 and, to a lesser extent, of SMS2 affected the formation of DAG at the Golgi apparatus. As a consequence, down-regulation of SMS1 and SMS2 reduced the localization of the DAG-binding protein, protein kinase D (PKD), to the Golgi. Since PKD recruitment to the Golgi has been implicated in cellular secretion through the trans golgi network (TGN), the effect of down-regulation of SMSs on TGN-to-plasma membrane trafficking was studied. Down regulation of either SMS1 or SMS2 significantly retarded trafficking of the reporter protein vesicular stomatitis virus G protein tagged with GFP (VSVG-GFP) from the TGN to the cell surface. Inhibition of SMSs also induced tubular protrusions from the trans Golgi network reminiscent of inhibited TGN membrane fission. Since a recent study demonstrated the requirement of PKD activity for insulin secretion in beta cells, we tested the function of SMS in this model. Inhibition of SMS significantly reduced insulin secretion in rat INS-1 cells. Taken together these results provide the first direct evidence that both enzymes (SMS1 and 2) are capable of regulating TGN-mediated protein trafficking and secretion, functions that are compatible with PKD being a down-stream target for SMSs in the Golgi

Role of Sphingomyelin Synthase in Controlling the Antimicrobial Activity of Neutrophils against Cryptococcus neoformans

The key host cellular pathway(s) necessary to control the infection caused by inhalation of the environmental fungal pathogen Cryptococcus neoformans are still largely unknown. Here we have identified that the sphingolipid pathway in neutrophils is required for them to exert their killing activity on the fungus. In particular, using both pharmacological and genetic approaches, we show that inhibition of sphingomyelin synthase (SMS) activity profoundly impairs the killing ability of neutrophils by preventing the extracellular release of an antifungal factor(s). We next found that inhibition of protein kinase D (PKD), which controls vesicular sorting and secretion and is regulated by diacylglycerol (DAG) produced by SMS, totally blocks the extracellular killing activity of neutrophils against C. neoformans. The expression of SMS genes, SMS activity and the levels of the lipids regulated by SMS (namely sphingomyelin (SM) and DAG) are up-regulated during neutrophil differentiation. Finally, tissue imaging of lungs infected with C. neoformans using matrix-assisted laser desorption-ionization mass spectrometry (MALDI-MS), revealed that specific SM species are associated with neutrophil infiltration at the site of the infection. This study establishes a key role for SMS in the regulation of the killing activity of neutrophils against C. neoformans through a DAG-PKD dependent mechanism, and provides, for the first time, new insights into the protective role of host sphingolipids against a fungal infection