MHC class II complexes sample intermediate states along the peptide exchange pathway

The presentation of peptide-MHCII complexes (pMHCIIs) for surveillance by T cells is a well-known immunological concept in vertebrates, yet the conformational dynamics of antigen exchange remain elusive. By combining NMR- detected H/D exchange with Markov modelling analysis of an aggregate of 275 microseconds molecular dynamics simulations, we reveal that a stable pMHCII spontaneously samples intermediate conformations relevant for peptide exchange. More specifically, we observe two major peptide exchange pathways: the kinetic stability of a pMHCII’s ground state defines its propensity for intrinsic peptide exchange, while the population of a rare, intermediate conformation correlates with the propensity of the HLA-DM-catalysed pathway. Helix-destabilizing mutants designed based on our model shift the exchange behaviour towards the HLA-DM-catalysed pathway and further allow us to conceptualize how allelic variation can shape an individual’s MHC restricted immune response

The Biological Standard of Living in the two Germanies.

Physical stature is used as a proxy for the biological standard of living in the two Germanies before and after unification in an analysis of a cross-sectional sample (1998) of adult heights, as well as among military recruits of the 1990s. West Germans tended to be taller than East Germans throughout the period under consideration. Contrary to official proclamations of a classless society, there were substantial social differences in physical stature in East-Germany. Social differences in height were greater in the East among females, and less among males than in the West. The difficulties experienced by the East-German population after 1961 is evident in the increase in social inequality of physical stature thereafter, as well as in the increasing gap relative to the height of the West-German population. After unification, however, there is a tendency for East-German males, but not of females, to catch up with their West-German counterparts

Memorandum on Mississippi House Bill 1523

As legal scholars with expertise in matters of religious freedom, civil rights, and the interaction between those fields, we offer our opinion on the scope and meaning of Mississippi House Bill 1523, which was signed into law today by Governor Phil Bryant. Specifically, we wish to call attention to language in the law that we believe conflicts with the Establishment Clause of the U.S. Constitution. We share the view of Justice Kennedy when he expressed that “a bare . . . desire to harm a politically unpopular group cannot constitute a legitimate governmental interest,” and would add that neither can such a desire be justified in the name of religious liberty. HB 1523 presents a conflict with First Amendment religious freedom doctrine by providing for religious exemptions that will meaningfully harm the rights of others, particularly LGBT Mississippians

Creating nearly Heisenberg-limited matter-waves exploiting tunable interactions

The wave nature of matter implies wavepackets with minimal combined uncertainty in position and momentum, a limit which can hardly be reached for clouds of large atom numbers of interacting particles. Here, we report on a high-flux source of ultra-cold atoms realizing near-Heisenberg-limited expansion rates upon release from the trap. Depending on the value of the scattering length, we model our system either with a scaling approach based on the Thomas-Fermi approximation, or with a variational approach based on a Gaussian atomic density approximation, observing the transition between the weak and strong interaction regimes. Finally, we discuss applications of our methods to test foundational principles of quantum mechanics such as the superposition principle or their extension to other atomic species

Matter-wave collimation to picokelvin energies with scattering length and potential shape control

We study the impact of atomic interactions on an in-situ collimation method for matter-waves. Building upon an earlier study with $^{87}$Rb, we apply a lensing protocol to $^{39}$K where the atomic scattering length can be tailored by means of magnetic Feshbach resonances. Minimizing interactions, we show an enhancement of the collimation compared to the strong interaction regime, realizing ballistic 2D expansion energies of 438(77) pK in our experiment. Our results are supported by an accurate simulation, describing the ensemble dynamics, which we further use to study the behavior of various trap configurations for different interaction strengths. Based on our findings we propose an advanced scenario which allows for 3D expansion energies below 16 pK by implementing an additional pulsed delta-kick collimation directly after release from the trapping potential. Our results pave the way to achieve state-of-the-art quantum state in typical dipole trap setups required to perform ultra-precise measurements without the need of complex micro-gravity or long baselines environments

Counting Complex Disordered States by Efficient Pattern Matching: Chromatic Polynomials and Potts Partition Functions

Counting problems, determining the number of possible states of a large system under certain constraints, play an important role in many areas of science. They naturally arise for complex disordered systems in physics and chemistry, in mathematical graph theory, and in computer science. Counting problems, however, are among the hardest problems to access computationally. Here, we suggest a novel method to access a benchmark counting problem, finding chromatic polynomials of graphs. We develop a vertex-oriented symbolic pattern matching algorithm that exploits the equivalence between the chromatic polynomial and the zero-temperature partition function of the Potts antiferromagnet on the same graph. Implementing this bottom-up algorithm using appropriate computer algebra, the new method outperforms standard top-down methods by several orders of magnitude, already for moderately sized graphs. As a first application, we compute chromatic polynomials of samples of the simple cubic lattice, for the first time computationally accessing three-dimensional lattices of physical relevance. The method offers straightforward generalizations to several other counting problems.Comment: 7 pages, 4 figure