591 research outputs found
Enzymatic reduction and oxidation of fibre-bound azo-dyes
A new customer and environmental friendly method of hair bound dye decolouration was developed. Biotransformation of the azo-dyes Flame Orange and Ruby Red was studied using different oxidoreductases. The pathways of azo dye conversion by these enzymes were investigated and the intermediates and metabolites were identified and characterised using UV–vis spectroscopy, high-performance liquid chromatography (HPLC) and mass spectrometry (MS). Laccase from Pycnoporus cinnabarinus, manganese peroxidase (MnP) from Nematoloma frowardii and the novel Agrocybe aegerita peroxidase (AaP) were found to use a similar mechanism to convert azo dyes. They N-demethylated the dyes and concomitantly polymerized them to some extent. On the other hand the mechanism for cleavage of the azo bond by azo-reductases of Bacillus cereus and B. subtilis was based on reduction of the azo bond at the expense of NAD(P)H
Coherent resonant tunneling in ac fields
We have analyzed the tunneling transmission probability and electronic
current density through resonant heterostructures in the presence of an
external electromagnetic field. In this work, we compare two different models
for a double barrier : In the first case the effect of the external field is
taken into account by spatially dependent AC voltages and in the second one the
electromagnetic field is described in terms of a photon field that irradiates
homogeneously the whole sample. While in the first description the tunneling
takes place mainly through photo sidebands in the case of homogeneous
illumination the main effective tunneling channels correspond to the coupling
between different electronic states due to photon absorption and emission. The
difference of tunneling mechanisms between these configurations is strongly
reflected in the transmission and current density which present very different
features in both cases. In order to analyze these effects we have obtained,
within the Transfer Hamiltonian framework, a general expression for the
transition probability for coherent resonant tunneling in terms of the Green's
function of the system.Comment: 16 pages,Figures available upon request,to appear in Phys.Rev B (15
April 1996
Coherent and sequential photoassisted tunneling through a semiconductor double barrier structure
We have studied the problem of coherent and sequential tunneling through a
double barrier structure, assisted by light considered to be present All over
the structure, i,e emitter, well and collector as in the experimental evidence.
By means of a canonical transformation and in the framework of the time
dependent perturbation theory, we have calculated the transmission coefficient
and the electronic resonant current. Our calculations have been compared with
experimental results turning out to be in good agreement. Also the effect on
the coherent tunneling of a magnetic field parallel to the current in the
presence of light, has been considered.Comment: Revtex3.0, 8figures uuencoded compressed tar-fil
Dynamical description of the buildup process in resonant tunneling: Evidence of exponential and non-exponential contributions
The buildup process of the probability density inside the quantum well of a
double-barrier resonant structure is studied by considering the analytic
solution of the time dependent Schr\"{o}dinger equation with the initial
condition of a cutoff plane wave. For one level systems at resonance condition
we show that the buildup of the probability density obeys a simple charging up
law, where is the
stationary wave function and the transient time constant is exactly
two lifetimes. We illustrate that the above formula holds both for symmetrical
and asymmetrical potential profiles with typical parameters, and even for
incidence at different resonance energies. Theoretical evidence of a crossover
to non-exponential buildup is also discussed.Comment: 4 pages, 2 figure
Non Linear Current Response of a Many-Level Tunneling System: Higher Harmonics Generation
The fully nonlinear response of a many-level tunneling system to a strong
alternating field of high frequency is studied in terms of the
Schwinger-Keldysh nonequilibrium Green functions. The nonlinear time dependent
tunneling current is calculated exactly and its resonance structure is
elucidated. In particular, it is shown that under certain reasonable conditions
on the physical parameters, the Fourier component is sharply peaked at
, where is the spacing between
two levels. This frequency multiplication results from the highly nonlinear
process of photon absorption (or emission) by the tunneling system. It is
also conjectured that this effect (which so far is studied mainly in the
context of nonlinear optics) might be experimentally feasible.Comment: 28 pages, LaTex, 7 figures are available upon request from
[email protected], submitted to Phys.Rev.
Outcomes and outcome measures used in evaluation of communication training in oncology - a systematic literature review, an expert workshop, and recommendations for future research
BACKGROUND: Communication between health care provider and patients in oncology presents challenges. Communication skills training have been frequently developed to address those. Given the complexity of communication training, the choice of outcomes and outcome measures to assess its effectiveness is important. The aim of this paper is to 1) perform a systematic review on outcomes and outcome measures used in evaluations of communication training, 2) discuss specific challenges and 3) provide recommendations for the selection of outcomes in future studies.
METHODS: To identify studies and reviews reporting on the evaluation of communication training for health care professionals in oncology, we searched seven databases (Ovid MEDLINE, CENTRAL, CINAHL, EMBASE, PsychINFO, PsychARTICLES and Web of Science). We extracted outcomes assessed and the respective assessment methods. We held a two-day workshop with experts (n = 16) in communication theory, development and evaluation of generic or cancer-specific communication training and/or outcome measure development to identify and address challenges in the evaluation of communication training in oncology. After the workshop, participants contributed to the development of recommendations addressing those challenges.
RESULTS: Out of 2181 references, we included 96 publications (33 RCTs, 2 RCT protocols, 4 controlled trials, 36 uncontrolled studies, 21 reviews) in the review. Most frequently used outcomes were participants\u27 training evaluation, their communication confidence, observed communication skills and patients\u27 overall satisfaction and anxiety. Outcomes were assessed using questionnaires for participants (57.3%), patients (36.0%) and observations of real (34.7%) and simulated (30.7%) patient encounters. Outcomes and outcome measures varied widely across studies. Experts agreed that outcomes need to be precisely defined and linked with explicit learning objectives of the training. Furthermore, outcomes should be assessed as broadly as possible on different levels (health care professional, patient and interaction level).
CONCLUSIONS: Measuring the effects of training programmes aimed at improving health care professionals\u27 communication skills presents considerable challenges. Outcomes as well as outcome measures differ widely across studies. We recommended to link outcome assessment to specific learning objectives and to assess outcomes as broadly as possible
Mutations in protocadherin 15 and cadherin 23 affect tip links and mechanotransduction in mammalian sensory hair cells
Immunocytochemical studies have shown that protocadherin-15 (PCDH15) and cadherin-23 (CDH23) are associated with tip links, structures thought to gate the mechanotransducer channels of hair cells in the sensory epithelia of the inner ear. The present report describes functional and structural analyses of hair cells from Pcdh15av3J (av3J), Pcdh15av6J (av6J) and Cdh23v2J (v2J) mice. The av3J and v2J mice carry point mutations that are predicted to introduce premature stop codons in the transcripts for Pcdh15 and Cdh23, respectively, and av6J mice have an in-frame deletion predicted to remove most of the 9th cadherin ectodomain from PCDH15. Severe disruption of hair-bundle morphology is observed throughout the early-postnatal cochlea in av3J/av3J and v2J/v2J mice. In contrast, only mild-to-moderate bundle disruption is evident in the av6J/av6J mice. Hair cells from av3J/av3J mice are unaffected by aminoglycosides and fail to load with [3H]-gentamicin or FM1-43, compounds that permeate the hair cell's mechanotransducer channels. In contrast, hair cells from av6J/av6J mice load with both FM1-43 and [3H]-gentamicin, and are aminoglycoside sensitive. Transducer currents can be recorded from hair cells of all three mutants but are reduced in amplitude in all mutants and have abnormal directional sensitivity in the av3J/av3J and v2J/v2J mutants. Scanning electron microscopy of early postnatal cochlear hair cells reveals tip-link like links in av6J/av6J mice, substantially reduced numbers of links in the av3J/av3J mice and virtually none in the v2J/v2J mice. Analysis of mature vestibular hair bundles reveals an absence of tip links in the av3J/av3J and v2J/v2J mice and a reduction in av6J/av6J mice. These results therefore provide genetic evidence consistent with PCDH15 and CDH23 being part of the tip-link complex and necessary for normal mechanotransduction
AC-conductance of a quantum wire with electron-electron interaction
The complex ac-response of a quasi-one dimensional electron system in the
one-band approximation with an interaction potential of finite range is
investigated. It is shown that linear response is exact for this model. The
influence of the screening of the electric field is discussed. The complex
absorptive conductance is analyzed in terms of resistive, capacitive and
inductive behaviors.Comment: 13 pages, REVTeX, 7 eps figures, to appear in Phys. Rev.
Giant Electroresistance in Edge Metal-Insulator-Metal Tunnel Junctions Induced by Ferroelectric Fringe Fields
An enormous amount of research activities has been devoted to developing new types of non-volatile memory devices as the potential replacements of current flash memory devices. Theoretical device modeling was performed to demonstrate that a huge change of tunnel resistance in an Edge Metal-Insulator-Metal (EMIM) junction of metal crossbar structure can be induced by the modulation of electric fringe field, associated with the polarization reversal of an underlying ferroelectric layer. It is demonstrated that single three-terminal EMIM/Ferroelectric structure could form an active memory cell without any additional selection devices. This new structure can open up a way of fabricating all-thin-film-based, high-density, high-speed, and low-power non-volatile memory devices that are stackable to realize 3D memory architectureope
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