36 research outputs found
Variations in DNA methylation of interferon gamma and programmed death 1 in allograft rejection after kidney transplantation
Identification of a long non-coding RNA-associated RNP complex regulating metastasis at the translational step
Analysis of Intraspecies Genetic Variability among Collections of Medicinal Red Belt Conk Mushroom, Fomitopsis pinicola (Agaricomycetes)
Antiproliferative Activity and Cytotoxicity of Some Medicinal Wood-Destroying Mushrooms from Russia
Genetic Resources and Mycelial Characteristics of Several Medicinal Polypore Mushrooms (Polyporales, Basidiomycetes)
Mycelial characteristics of dikaryotic collections of 6 medicinal polypore mushrooms (Fomes fomentarius, Fomitopsis pinicola, Ganoderma adspersum, G. applanatum, G. lucidum, and G. resinaceum) with different geographical origins (Armenia, China, France, Iran, Italy, and Russia) were screened. A total of 42 polypore collections were molecularly identified by sequencing the internal transcribed spacer region of the ribosomal RNA genes' cluster, and a phylogenetic tree was constructed. Morphological characteristics of 37 cultures were observed on agar media (malt extract agar, potato dextrose agar) at different temperatures (25, 30, 35, and 38\ub0C) at a pH of 6.0. Colony morphology, pigmentation of mycelium and agar, mycelial growth rate, in vitro teleomorph formation, and other macromorphological characteristics were thoroughly described and illustrated. Micromorphological features of mycelia, such as different hyphal structures, clamp cells, presence and type of asexual sporulation, chlamydospores, and others were observed. The taxonomic significance of the mycelial characteristics revealed was estimated. The obtained results will assist further biotechnological cultivation of medicinal polypore mushrooms to develop novel health care biotechnological products
Tandem organization and highly disparate expression of the two laccase genes lcc1 and lcc2 in the cultivated mushroom Agaricus bisporus
Permission to Proceed: Jak3 and STAT5 Signaling Molecules Give the Green Light for T Helper 1 Cell Differentiation
Evolutionarily conserved sequence elements that positively regulate IFN-γ expression in T cells
Our understanding of mechanisms by which the expression of IFN-γ is regulated is limited. Herein, we identify two evolutionarily conserved noncoding sequence elements (IFNgCNS1 and IFNg CNS2) located ≈5 kb upstream and ≈18 kb downstream of the initiation codon of the murine Ifng gene. When linked to the murine Ifng gene (–3.4 to +5.6 kb) and transiently transfected into EL-4 cells, these elements clearly enhanced IFN-γ expression in response to ionomycin and phorbol 12-myristate 13-acetate and weakly enhanced expression in response to T-bet. A DNase I hypersensitive site and extragenic transcripts at IFNgCNS2 correlated positively with the capacity of primary T cell subsets to produce IFN-γ. Transcriptionally favorable histone modifications in the Ifng promoter, intronic regions, IFNgCNS2, and, although less pronounced, IFNgCNS1 increased as naïve T cells differentiated into IFN-γ-producing effector CD8(+) and T helper (TH) 1 T cells, but not into TH2 T cells. Like IFN-γ expression, these histone modifications were T-bet-dependent in CD4(+) cells, but not CD8(+) T cells. These findings define two distal regulatory elements associated with T cell subset-specific IFN-γ expression