Primer informe de Aelurostrongylus abstrusus en el caracol de tierra Rumina decollata, en la Ciudad Autónoma de Buenos Aires

Aelurostrongylus abstrusus (Railliet, 1898) is a worldwide distributed lungworm that affects wild and domestic cats, causing bronchopneumonia of varying intensity. Cats became infected by eating slugs and snails with third infective stage larvae (L3). The aim of the study was to describe the presence of A. abstrusus in R. decollate snails. R. decollata specimens and samples of cats’ faeces were collected from the open spaces of a public institution of Buenos Aires city, inhabited by a stray cat population. Cats’ faeces were processed by Baermman´s technique and snails were digested in pool, by artificial digestion method. First stage larvae of A. abstrusus were recovered from 35.30 % (6/17) of the sampled faeces. An 80 % (20/25) snails pools were positive for the second and third larval stages. Mean value of total larvae recovered per pool was 150.64 and mean value of L3/pool was 93.89. This is the first report of the development of A. abstrusus infective larvae in R. decollate snail as intermediate host, since the relationship between high levels of infection in snails and in cats’ faeces could be demonstrated in cats’ habitat.Aelurostrongylus abstrusus (Railliet, 1898) es un helminto pulmonar mundialmente distribuido que afecta a los gatos, causando bronconeumonias de variada intensidad. La infección se produce por ingestión de babosas y caracoles terrestres con larvas infectantes (L3). El objetivo del estudio fue describir la presencia de A. abstrusus en el caracol R. decollata. Se recolectaron muestras de heces felinas y caracoles presentes en una institución pública de la Ciudad Autónoma de Buenos Aires, habitada por una población de gatos sin propietario. Las heces fueron procesadas mediante la técnica de Baermman y los caracoles fueron digeridos en pool por digestión artificial enzimática. Larvas de primer estadio (L1) de A. abstrusus fueron recuperadas en el 35,30% (6/17) de las heces. El 80% (20/25) de los pooles de caracoles presentó larvas de segundo y tercer estadio. El promedio de larvas totales recuperado por pool fue de 150,64 y el valor medio de L3/pool fue de 93.89. Este es el primer hallazgo del desarrollo de larvas infectivas de A. abstrusus en el caracol doméstico R. decollata. Los altos niveles de infección encontrados en los caracoles y en las heces de los gatos demuestran el potencial de R. decollata como hospedador intermediario de A. abstrusus.Fil: Cardillo, Natalia Marina. Universidad de Buenos Aires. Facultad de Cs.veterinarias. Area de Parasitología y Enfermedades Parasitarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Clemente, A.. Universidad de Buenos Aires. Facultad de Cs.veterinarias. Area de Parasitología y Enfermedades Parasitarias; ArgentinaFil: Pasqualetti, M.. Universidad de Buenos Aires. Facultad de Cs.veterinarias. Area de Parasitología y Enfermedades Parasitarias; ArgentinaFil: Borrás, P.. Universidad de Buenos Aires. Facultad de Cs.veterinarias. Area de Parasitología y Enfermedades Parasitarias; ArgentinaFil: Rosa, A.. Universidad de Buenos Aires. Facultad de Cs.veterinarias. Area de Parasitología y Enfermedades Parasitarias; ArgentinaFil: Ribicich, M.. Universidad de Buenos Aires. Facultad de Cs.veterinarias. Area de Parasitología y Enfermedades Parasitarias; Argentin

“Know to prevent” in Northern Patagonia, Argentina

Fil: Winter, Marina. Universidad Nacional de Río Negro. Sede Atlántica; ArgentinaFil: Winter, Marina. Centro de Investigaciones y Transferencia Río Negro (CONICET-UNRN); ArgentinaFil: Abate, Sergio D. Centro de Investigaciones y Transferencia Río Negro (CONICET-UNRN); ArgentinaFil: Abate, Sergio D. Universidad Nacional de Río Negro. Sede Atlántica; ArgentinaFil: Pasqualetti, Mariana I. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pasqualetti, Mariana I. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Parasitología y Enfermedades Parasitarias; ArgentinaFil: Pasqualetti, Mariana I. Instituto de Investigaciones en Producción Animal (INPA); ArgentinaFil: Fariña, Fernando A. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fariña, Fernando A. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Parasitología y Enfermedades Parasitarias; ArgentinaFil: Fariña, Fernando A. Instituto de Investigaciones en Producción Animal (INPA); ArgentinaFil: Ribicich, Mabel M. Instituto de Investigaciones en Producción Animal (INPA); ArgentinaFil: Ribicich, Mabel M. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Parasitología y Enfermedades Parasitarias; ArgentinaFil: Ribicich, Mabel M. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaTrichinellosis is endemic in Argentina and an important public health problem because of its high morbidity rates. In Patagonia Argentina, despite not being an important pig production area, there are outbreaks of trichinellosis due to the consumption without bromatological control of pigs meat produced domestically and wild animals meat obtained by hunting activity. Also, the production of sausages (“chacinados’’) for family consumption and informal sale contributes to the transmission. Even though one way to prevent trichinellosis is to cook meat to safe temperatures, the consumption of raw sausages should only be made after the artificial digestion method was performed on animal carcasses and a negative result was given. In this context, the aim of this study was to make workshops for children between 6 and 12 years old, tending to provide tools that reduce the appearance of outbreaks of trichinellosis in a rural area of the northern Patagonia Argentina. Under the concept of "know to prevent", the different aspects of the parasite-host-environment triad were worked on. Between August 2016 and December 2017, seven groups of students between 6 and 12 years old of two primary rural schools took part in workshops. To stimulate learning, parasites were observed through a microscope and magnifying glass. They were developed theoretical content and practical, creative and playful activities. In addition, attractive and easy-to-understand brochures were designed for children and families. As a result, 176 children from two rural primary schools in a rural area of the northern Patagonia Argentina learned for the first time what trichinellosis is, how it is transmitted and how it is prevented. To promote a necessary change of attitude tending to prevent trichinellosis, the active participation of children is essential

Primer informe de Aelurostrongylus abstrusus en el caracol de tierra Rumina decollata, en la Ciudad Autónoma de Buenos Aires

Aelurostrongylus abstrusus (Railliet, 1898) is a worldwide distributed lungworm that affects wild\nand domestic cats, causing bronchopneumonia of varying intensity. Cats became infected by\neating slugs and snails with third infective stage larvae (L3). The aim of the study was to describe\nthe presence of A. abstrusus in R. decollate snails. R. decollata specimens and samples of cats?\nfaeces were collected from the open spaces of a public institution of Buenos Aires city, inhabited\nby a stray cat population. Cats? faeces were processed by Baermman´s technique and snails were\ndigested in pool, by artificial digestion method. First stage larvae of A. abstrusus were recovered\nfrom 35.30 % (6/17) of the sampled faeces. An 80 % (20/25) snails pools were positive for the\nsecond and third larval stages. Mean value of total larvae recovered per pool was 150.64 and\nmean value of L3/pool was 93.89. This is the first report of the development of A. abstrusus\ninfective larvae in R. decollate snail as intermediate host, since the relationship between high\nlevels of infection in snails and in cats? faeces could be demonstrated in cats? habitat.Fil: Cardillo, N. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Parasitología y Enfermedades Parasitarias; ArgentinaFil: Clemente, A. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Parasitología y Enfermedades Parasitarias; ArgentinaFil: Pasqualetti, M. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Parasitología y Enfermedades Parasitarias; ArgentinaFil: Borrás, P. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Parasitología y Enfermedades Parasitarias; ArgentinaFil: Rosa, A. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Parasitología y Enfermedades Parasitarias; ArgentinaFil: Ribicich, M. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Parasitología y Enfermedades Parasitarias; ArgentinaAelurostrongylus abstrusus (Railliet, 1898) es un helminto pulmonar mundialmente distribuido\nque afecta a los gatos, causando bronconeumonias de variada intensidad. La infección se produce\npor ingestión de babosas y caracoles terrestres con larvas infectantes (L3). El objetivo del estudio\nfue describir la presencia de A. abstrusus en el caracol R. decollata. Se recolectaron muestras de\nheces felinas y caracoles presentes en una institución pública de la Ciudad Autónoma de Buenos\nAires, habitada por una población de gatos sin propietario. Las heces fueron procesadas mediante\nla técnica de Baermman y los caracoles fueron digeridos en pool por digestión artificial enzimática.\nLarvas de primer estadio (L1) de A. abstrusus fueron recuperadas en el 35,30% (6/17) de las\nheces. El 80% (20/25) de los pooles de caracoles presentó larvas de segundo y tercer estadio.\nEl promedio de larvas totales recuperado por pool fue de 150,64 y el valor medio de L3/pool\nfue de 93.89. Este es el primer hallazgo del desarrollo de larvas infectivas de A. abstrusus en el\ncaracol doméstico R. decollata. Los altos niveles de infección encontrados en los caracoles y en\nlas heces de los gatos demuestran el potencial de R. decollata como hospedador intermediario\nde A. abstrusus

Trichinellosis surveillance in wildlife in northeastern argentine patagonia

Fil: Winter, Marina. Universidad Nacional de Río Negro, Sede Atlántica; ArgentinaFil: Winter, Marina. Centro de Investigaciones y Transferencia Río Negro (CONICET-UNRN); ArgentinaFil: Pasqualetti, Mariana I. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Parasitología y Enfermedades Parasitarias; ArgentinaFil: Pasqualetti, Mariana I. Instituto de Investigaciones en Producción Animal (INPA); ArgentinaFil: Pasqualetti, Mariana I. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fariña, Fernando A. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Parasitología y Enfermedades Parasitarias; ArgentinaFil: Fariña, Fernando A. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fariña, Fernando A. Instituto de Investigaciones en Producción Animal (INPA); ArgentinaFil: Ercole, Mariano E. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Parasitología y Enfermedades Parasitarias; ArgentinaFil: Failla, Mauricio. Proyecto Patagonia Noreste; ArgentinaFil: Failla, Mauricio. Museo Provincial Patagónico de Ciencias Naturales Juan Carlos Salgado; ArgentinaFil: Perello, Mario C. Fundación Félix de Azara; ArgentinaFil: Birochio, Diego E. Universidad Nacional de Río Negro, Sede Atlántica; ArgentinaFil: Birochio, Diego E. Centro de Investigaciones y Transferencia Río Negro; ArgentinaFil: Abate, Sergio D. Universidad Nacional de Río Negro, Sede Atlántica; ArgentinaFil: Abate, Sergio D. Centro de Investigaciones y Transferencia; ArgentinaFil: Soricetti, Mariano. Universidad Nacional de Río Negro, Sede Atlántica; ArgentinaFil: Soricetti, Mariano. Centro de Investigaciones y Transferencia; ArgentinaFil: Ribicich, Mabel M. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Parasitología y Enfermedades Parasitarias; ArgentinaFil: Ribicich, Mabel M. Instituto de Investigaciones en Producción Animal (INPA); ArgentinaFil: Ribicich, Mabel M. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaTrichinellosis is a food-borne parasitic disease produced by different nematodes of the genus Trichinella. In Argentina, it is an endemic zoonosis and an important public health problem. The infection has been detected in domestic and wild animals. Trichinella spp. muscle larvae have anaerobic metabolism, which allows their survival in decaying tissues. The aim of this study was to evaluate the presence of Trichinella spp. in carnivorous and/or scavenger wild vertebrates - birds, mammals and reptiles - in northeastern Argentine Patagonia. Skeletal muscle samples from 141 animals, which were found killed on northeastern Argentine Patagonia roads, were analyzed by the artificial digestion method. None of the 141 samples were positive for larvae of Trichinella. These results suggest that Trichinella does not use these species to complete its cycle in this region of the continent and the absence of a significant alteration in the study area makes it difficult to transmit parasitic diseases. However, due to the limited number of samples assessed for some species, this could not be confirmed. The relevance of this study resides in the fact that it is the first systematic study in South America that considers birds, reptiles and mammals as potential hosts for Trichinella.Trichinellosis is a food-borne parasitic disease produced by different nematodes of the genus Trichinella. In Argentina, it is an endemic zoonosis and an important public health problem. The infection has been detected in domestic and wild animals. Trichinella spp. muscle larvae have anaerobic metabolism, which allows their survival in decaying tissues. The aim of this study was to evaluate the presence of Trichinella spp. in carnivorous and/or scavenger wild vertebrates - birds, mammals and reptiles - in northeastern Argentine Patagonia. Skeletal muscle samples from 141 animals, which were found killed on northeastern Argentine Patagonia roads, were analyzed by the artificial digestion method. None of the 141 samples were positive for larvae of Trichinella. These results suggest that Trichinella does not use these species to complete its cycle in this region of the continent and the absence of a significant alteration in the study area makes it difficult to transmit parasitic diseases. However, due to the limited number of samples assessed for some species, this could not be confirmed. The relevance of this study resides in the fact that it is the first systematic study in South America that considers birds, reptiles and mammals as potential hosts for Trichinella

Trichinella spiralis natural infection in Otaria flasvecens from Patagonia, Argentina

Fil: Pasqualetti, Mariana I. Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Cátedra de Parasitología y Enfermedades Parasitarias; ArgentinaFil: Pasqualetti, Mariana I. Instituto de Investigaciones en Producción Animal (INPA); ArgentinaFil: Pasqualetti, Mariana I. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fariña, Fernando A. Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Cátedra de Parasitología y Enfermedades Parasitarias; ArgentinaFil: Fariña, Fernando A. Instituto de Investigaciones en Producción Animal (INPA); ArgentinaFil: Fariña, Fernando A. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Krivokapich, Silvio J. Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"; ArgentinaFil: Gatti, Genoveva M. Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"; ArgentinaFil: Daneri, Gustavo A. Laboratorio de Sistemática, Anatomía y Bioecología de Mamíferos Marinos, Division Mastozoología, Museo Argentino de CienciasNaturales,“Bernardino Rivadavia”; ArgentinaFil: Varela, Esperanza A. Laboratorio de Sistemática, Anatomía y Bioecología de Mamíferos Marinos, Division Mastozoología, Museo Argentino de CienciasNaturales,“Bernardino Rivadavia”; ArgentinaFil: Lucero, Sergio. Laboratorio de Sistemática, Anatomía y Bioecología de Mamíferos Marinos, Division Mastozoología, Museo Argentino de CienciasNaturales,“Bernardino Rivadavia”; ArgentinaFil: Ercole, Mariano E. Universidad de Buenos Aires, Facultad de Ciencias Veterinarias,Cátedra de Parasitología y Enfermedades Parasitarias; ArgentinaFil: Bessi, Clara. Universidad de Buenos Aires, Facultad de Ciencias Veterinarias,Cátedra de Parasitología y Enfermedades Parasitarias; ArgentinaFil: Bessi, Clara. Instituto de Investigaciones en Producción Animal (INPA); ArgentinaFil: Bessi, Clara. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Winter, Marina. Universidad Nacional de Río Negro. Sede Atlántica; ArgentinaFil: Winter, Marina. Centro de Investigación y Transferencia Río Negro; ArgentinaFil: Ribicich, Mabel M. Instituto de Investigaciones en Producción Animal (INPA); ArgentinaFil: Ribicich, Mabel M. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Parasitología y Enfermedades Parasitarias; ArgentinaFil: Ribicich, Mabel M. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaIn Argentina trichinellosis is an endemic disease representing an important risk for human health due to its high rates of morbidity, mainly transmitted by the consumption of raw or undercooked pork. Nevertheless, the discovery of new Trichinella species have led to a change in the study of the epidemiology of the disease with the addition of new sources of infection. Moreover, Trichinella infection has been detected in a wide range of marine mammals around the world. Until the present time, Trichinella spp. infection has not been detected in marine mammals of South America. Four South American sea lions were found dead in the rookeries of Caleta de los Loros (Lat. 41° 00′ S; 64° 12′ W; n = 1), Promontorio Belén (Lat. 41° 09′ S; Long. 63° 48′ O; n = 1) and Punta Bermeja (Lat. 41° 09′ S; Long. 63° 09′ O; n = 2) in Rio Negro, Argentina. Muscle samples were taken from the tongue and diaphragm and were stored at 4 °C until examination at the Parasitology Laboratory of the Facultad de Ciencias Veterinarias, Universidad de Buenos Aires. The total muscle samples from each animal were analyzed by artificial digestión. Identification at the species level was made by nested multiplex chain reaction (nested multiplex PCR) based on nuclear ribosomal DNA sequences, using six pair of primers. Trichinella spp. larvae were found in one of the four South American sea lions. Based on their morphology, the recovered larvae were suggestive of Trichinella spp. Trichinella larvae generated a fragment of 173 bp corresponding to T. spiralis expansion segment V (ESV) region of the ribosomal DNA. This is the first report of a Trichinella species infecting marine mammals from South America. The inclusion of Otaria flavescens in the wide range of Trichinella hosts adds new questions to the epidemiology of Trichinella in marine animals. We thank the Secretaría de Medio Ambiente y Desarrollo Sustentable (SAyDS) of Río Negro Province for giving us permission to get sample from the rookeries. This work was supported by Universidad de Buenos Aires, Secretaria de Ciencia y Técnica Subsidio UBACyT20020130100336BA and Ministerio de Ciencia y Tecnología, FONCyT Subsidio PICT-2015-235

A review on the inactivation methods of parasites present in fish intended for human consumption

La prevención y el control de las parasitosis constituyen eslabones fundamentales en las diferentes etapas del proceso productivo de la industria pesquera, debido a que estos agentes pueden causar importantes problemas económicos y riesgos para la salud. Existen ciertas especies de parásitos que poseen un efecto devastador en la producción acuícola, ya que encuentran en las estaciones de cría, el lugar ideal para reproducirse. En consecuencia, es necesario utilizar tratamientos de inactivación adecuados para garantizar la calidad del producto alimenticio, conservar sus características organolépticas y evitar el contagio de las personas por parásitos zoonóticos. Se realizó una búsqueda bibliográfica con el objetivo de describir los principales métodos de inactivación de parásitos zoonóticos reportadosen Argentina. La congelación, cocción, salazón, marinado, ahumado e irradiación constituyen tratamientos postulados para asegurar la inocuidad de diversos productos y subproductos pesqueros cuando son aplicados bajo determinadas condiciones. Las regulaciones sobre parasitosis, guías y documentos para la industria y educación de los consumidores constituyen estrategias fundamentales para producir alimentos seguros para la población.The prevention and control of parasites are essential tasks in the fishing industry that tend to avoid significant economic problems and health risks. There exists some parasites that have a devastating effect on aquaculture, since they find the ideal place to reproduce in the breeding stations. Consequently, it is necessary to use appropriate inactivation methods on fish to guarantee the quality of the food product, preserve its organoleptic characteristics and avoid the contagion of people by zoonotic parasites. To this end, a bibliographic search was carried out in order to describe the main methods of inactivation of zoonotic fish borne parasites reported in Argentina. Freezing, cooking, salting, marinating, smoking and irradiation are postulated as possible treatments to ensure the safety of various fish products and by-products when they are applied under certain conditions. Regulations on parasites, guides and documents for industry and consumer education constitute fundamental strategies to produce safe food for the population.Fil: Fariña, Fernando Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Área de Parasitología y Enfermedades Parasitarias; ArgentinaFil: Pasqualetti, Mariana Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Área de Parasitología y Enfermedades Parasitarias; ArgentinaFil: Aronowicz, Tatiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Área de Parasitología y Enfermedades Parasitarias; ArgentinaFil: Ercole, Mariano Emmanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; ArgentinaFil: Bessi, Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Área de Parasitología y Enfermedades Parasitarias; ArgentinaFil: Alvarez, M.. Ministerio de Agricultura Ganadería y Pesca de la Nación; Argentina. Universidad Nacional de Moreno; ArgentinaFil: Lopez, F.. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Volpedo, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; ArgentinaFil: Ribicich, Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Área de Parasitología y Enfermedades Parasitarias; Argentin

Toxoplasma gondii and <i>Trichinella</i> infections in wild boars (<i>Sus scrofa</i>) from Northeastern Patagonia, Argentina

Wild boar (Sus scrofa) was introduced in many countries of the world and is recognized as carrier of many infectious diseases. Wild game meat consumption is recognized as a source of transmission of Toxoplasma gondii and Trichinella spp. The aim of the present study was to evaluate the prevalence of antibodies to T. gondii and Trichinella spp. in free-range wild boars in Northeastern Argentine Patagonia. Between 2014 and 2018, 144 blood samples and 423 muscle samples from 423 carcasses were collected. To detect T. gondii IgG, 144 sera were processed by an immunofluorescent antibody test, and to detect anti-Trichinella IgG, 125 sera and 304 muscle juice samples were processed by ELISA. Detection of first stage larvae in muscle was performed by artificial digestion . A total of 423 wild boars muscle samples were negative to Trichinella spp. by artificial digestion. Antibodies to Trichinella spp. were detected in 2.4% (3/125) of serum samples and in 1.64% (5/304) of meat juice samples. Antibodies to T. gondii infection were detected in 12.5% (18/144) of the serum samples. This is the first study to reveal the presence of antibodies to T. gondii in wild boars from Argentina. The present results suggest that consumption of raw or undercooked wild boar meat could represent a potential source risk for toxoplasmosis in humans and that Trichinella spp. is infrequent and/or that it circulates in low burdens among wild boars in Northeastern Patagonia.Laboratorio de Inmunoparasitologí

Toxoplasma gondii and <i>Trichinella</i> infections in wild boars (<i>Sus scrofa</i>) from Northeastern Patagonia, Argentina

Wild boar (Sus scrofa) was introduced in many countries of the world and is recognized as carrier of many infectious diseases. Wild game meat consumption is recognized as a source of transmission of Toxoplasma gondii and Trichinella spp. The aim of the present study was to evaluate the prevalence of antibodies to T. gondii and Trichinella spp. in free-range wild boars in Northeastern Argentine Patagonia. Between 2014 and 2018, 144 blood samples and 423 muscle samples from 423 carcasses were collected. To detect T. gondii IgG, 144 sera were processed by an immunofluorescent antibody test, and to detect anti-Trichinella IgG, 125 sera and 304 muscle juice samples were processed by ELISA. Detection of first stage larvae in muscle was performed by artificial digestion . A total of 423 wild boars muscle samples were negative to Trichinella spp. by artificial digestion. Antibodies to Trichinella spp. were detected in 2.4% (3/125) of serum samples and in 1.64% (5/304) of meat juice samples. Antibodies to T. gondii infection were detected in 12.5% (18/144) of the serum samples. This is the first study to reveal the presence of antibodies to T. gondii in wild boars from Argentina. The present results suggest that consumption of raw or undercooked wild boar meat could represent a potential source risk for toxoplasmosis in humans and that Trichinella spp. is infrequent and/or that it circulates in low burdens among wild boars in Northeastern Patagonia.Laboratorio de Inmunoparasitologí

Localization of Trichinella spiralis in muscles of commercial and parasitologic interest in pork

Trichinellosis is widespread around the world with different representatives of the genus Trichinella found in almost every continent. In Argentina the main source of transmission for the disease to humans is pig meat infected with Trichinella spiralis. The object of this work was to determine the distribution of Trichinella larvae in fresh meat cuts which are sold for human consumption and in the muscles traditionally used for the disease diagnosis at meat-packing plants. Cranial muscles to the last rib showed more Trichinella spiralis larvae than those with a caudal location (p 0.05) between bilateral left and right muscles. Significant larval concentrations were found in the neck muscles, even in carcasses with a low parasitic load; these muscles are used to prepare cold meats (boston butt). Commercial cuts of meat had a substantial larval burdens in animals experimentally infected with 500 to 5,000 Trichinella spiralis larvae, with parasite burdens similar to infection levels in muscles evaluated at the meat packing plant

Localization of

Trichinellosis is widespread around the world with different representatives of the genus Trichinella found in almost every continent. In Argentina the main source of transmission for the disease to humans is pig meat infected with Trichinella spiralis. The object of this work was to determine the distribution of Trichinella larvae in fresh meat cuts which are sold for human consumption and in the muscles traditionally used for the disease diagnosis at meat-packing plants. Cranial muscles to the last rib showed more Trichinella spiralis larvae than those with a caudal location (p 0.05) between bilateral left and right muscles. Significant larval concentrations were found in the neck muscles, even in carcasses with a low parasitic load; these muscles are used to prepare cold meats (boston butt). Commercial cuts of meat had a substantial larval burdens in animals experimentally infected with 500 to 5,000 Trichinella spiralis larvae, with parasite burdens similar to infection levels in muscles evaluated at the meat packing plant