62 research outputs found
Ichemic injury on early post-transplant endomyocardial Biopsy: Immediate and long-term clinical significance
The Amino-Terminus of Nitric Oxide Sensitive Guanylyl Cyclase α1 Does Not Affect Dimerization but Influences Subcellular Localization
BACKGROUND: Nitric oxide sensitive guanylyl cyclase (NOsGC) is a heterodimeric enzyme formed by an α- and a ÎČâ-subunit. A splice variant (C-αâ) of the αâ-subunit, lacking at least the first 236 amino acids has been described by Sharina et al. 2008 and has been shown to be expressed in differentiating human embryonic cells. Wagner et al. 2005 have shown that the amino acids 61-128 of the αâ-subunit are mandatory for quantitative heterodimerization implying that the C-αâ-splice variant should lose its capacity to dimerize quantitatively. METHODOLOGY/PRINCIPAL FINDINGS: In the current study we demonstrate preserved quantitative dimerization of the C-αâ-splice by co-purification with the ÎČâ-subunit. In addition we used fluorescence resonance energy transfer (FRET) based on fluorescence lifetime imaging (FLIM) using fusion proteins of the ÎČâ-subunit and the αâ-subunit or the C-αâ variant with ECFP or EYFP. Analysis of the respective combinations in HEK-293 cells showed that the fluorescence lifetime was significantly shorter (â0.3 ns) for αâ/ÎČâ and C-αâ/ÎČâ than the negative control. In addition we show that lack of the amino-terminus in the αâ splice variant directs it to a more oxidized subcellular compartment. CONCLUSIONS/SIGNIFICANCE: We conclude that the amino-terminus of the αâ-subunit is dispensable for dimerization in-vivo and ex-vivo, but influences the subcellular trafficking
Experimental traumatic brain injury
Traumatic brain injury, a leading cause of death and disability, is a result of an outside force causing mechanical disruption of brain tissue and delayed pathogenic events which collectively exacerbate the injury. These pathogenic injury processes are poorly understood and accordingly no effective neuroprotective treatment is available so far. Experimental models are essential for further clarification of the highly complex pathology of traumatic brain injury towards the development of novel treatments. Among the rodent models of traumatic brain injury the most commonly used are the weight-drop, the fluid percussion, and the cortical contusion injury models. As the entire spectrum of events that might occur in traumatic brain injury cannot be covered by one single rodent model, the design and choice of a specific model represents a major challenge for neuroscientists. This review summarizes and evaluates the strengths and weaknesses of the currently available rodent models for traumatic brain injury
In-line filtration minimizes organ dysfunction: New aspects from a prospective, randomized, controlled trial
Sildenafil, a cyclic GMP phosphodiesterase inhibitor, induces microglial modulation after focal ischemia in the neonatal mouse brain
Studentsâ informal statistical inferences through data modeling with a large multivariate dataset
This is the author accepted manuscript. The final version is available from Routledge via the DOI in this recordIn todayâs age of information, the use of data is very powerful in making informed decisions. Data analytics is a field that is interested in identifying and interpreting trends and patterns within big data to make data-driven decisions. We focus on informal statistical inference and data modeling as a means of developing studentsâ data analytics skills in school. In this study, we examine how students apply the data modeling process to draw informal inferences when exploring trends, patterns and relationships in a real dataset using technological tools, such as CODAP and Excel. We analyzed 17â18-year-old studentsâ written reports on their explorations of data supplied by third parties. Students used a variety of statistical measures and visualizations to account for variability in analyzing data. They tended to make statements with certainty in their inferences and predictions beyond the data. When the pattern in the data was uncertain, they were inclined to use contextual knowledge to remain certain in their claims.European Commissio
Neuropsychological performance in depressed and non-depressed patients with obsessive-compulsive disorder
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