Structure determination of a fluorescent product of the alkaline hydrolysis of pralidoxime

Ispitivanjem raznih svojstava stanica nakon tretmana rafinatom nafte nisu nađene jednoznačne promjene, koje bi upućivale na to da su tretirane stanice poprimile nove elemente malignog ponašanja. Tretirane stanice nisu pokazivale smanjenu kontaktnu lnhlblciju, niti im se signifikantno povećala sposobnost stvaranja tumora nakon injiciranja u ozračene miševe. Brzina rasta i trajanje rasta stanica u jednoslojnim kulturama ostali su također nepromijenjeni. Pozitivni nalazi odnosili su se na uočljive morfološke promjene svih tretiranih stanica, te na stečenu sposobnost C3Hf stanica da nakon tretmana rastu u mekom agaru i stvaraju tumore u miševima. Budući da je radiosenzitivnost stanica znatnim dijelom određena genetski kontroliranim reparacijskim procesima, to su također tražene i promjene u radiosenzitivnosti onih stanica koje su proistekle iz kultura ranije izlaganih navedenom naftnom derivatu. Uočene su promjene oblika krivulja preživljenja stanica tretiranih kultura, što se pripisuje djelovanju rafinata nafte na reparacijske procese.In the examination of various cellular properties after the treatment of cells with a crude oil distillate unambiguous changes which would indicate that the treated cells adopted new elements of malignant behaviour were not found. The treated cells demonstrated neither a decreased contact inhibition nor an increased ability to develop tumours after they had been injected to irradiated mice. The rate and duration of cell growth in mono-layer cultures remained unchanged too. Positive findings related to the morphological changes observed in all treated cells, and to the acquired ability of C3Hf cells after the treatment to grow in soft agar and to develop tumours in mice. Since cellular radiosensitivity is largely determined by genetically reparative processes we also looked for changes in radiosensitivity of the cells which had been earlier exposed to the crude oil distillate. In the treated cultures changes in the shape of the survival curves were observed, what is attributed to the effect of the crude oil distillate on reparative processes

Anti-cancer potential of MAPK pathway inhibition in paragangliomas-effect of different statins on mouse pheochromocytoma cells.

To date, malignant pheochromocytomas and paragangliomas (PHEOs/PGLs) cannot be effectively cured and thus novel treatment strategies are urgently needed. Lovastatin has been shown to effectively induce apoptosis in mouse PHEO cells (MPC) and the more aggressive mouse tumor tissue-derived cells (MTT), which was accompanied by decreased phosphorylation of mitogen-activated kinase (MAPK) pathway players. The MAPK pathway plays a role in numerous aggressive tumors and has been associated with a subgroup of PHEOs/PGLs, including K-RAS-, RET-, and NF1-mutated tumors. Our aim was to establish whether MAPK signaling may also play a role in aggressive, succinate dehydrogenase (SDH) B mutation-derived PHEOs/PGLs. Expression profiling and western blot analysis indicated that specific aspects of MAPK-signaling are active in SDHB PHEOs/PGLs, suggesting that inhibition by statin treatment could be beneficial. Moreover, we aimed to assess whether the anti-proliferative effect of lovastatin on MPC and MTT differed from that exerted by fluvastatin, simvastatin, atorvastatin, pravastatin, or rosuvastatin. Simvastatin and fluvastatin decreased cell proliferation most effectively and the more aggressive MTT cells appeared more sensitive in this respect. Inhibition of MAPK1 and 3 phosphorylation following treatment with fluvastatin, simvastatin, and lovastatin was confirmed by western blot. Increased levels of CASP-3 and PARP cleavage confirmed induction of apoptosis following the treatment. At a concentration low enough not to affect cell proliferation, spontaneous migration of MPC and MTT was significantly inhibited within 24 hours of treatment. In conclusion, lipophilic statins may present a promising therapeutic option for treatment of aggressive human paragangliomas by inducing apoptosis and inhibiting tumor spread

MODERN TRENDS IN FERTILITY PATTERNS IN UKRAINE

The paper describes the characteristics of a modern model of fertility and family planning in Ukraine. The transformation of the age model of fertility and changes in the structure of fertility by birth order have been describes. Modern features of the birth of children in Ukraine and some European countries have been compared