Expression of infectious bovine rhinotracheitis virus glycoprotein D in bacterial cell

Bovine Herpesvirus 1 (BHV-1) belongs to the genus of Varicellovirus and the family of Herpesviridae which contains three main gB, gC and gD genes. In order to cloning of the coding region of gD gene of IBR virus , PCR product of the open reading frame of the gene from IBR virus isolated in Iran was amplified by PCR. A 1047bp PCR product of the gD gene with EcoRI, HindIII restriction sites were subcloned of pTZ57R/T and digested by the mentioned endonucleases. Digested insert cloned in to pET-32a and transfered in E.coli cells. For the expression of gD protein, the pET-32a recombinant vector was transformed and then induced in BL21 (DE3) strain of E.coli competent cells using IPTG. The presence of gD expressed protein was shown in immunoblotting and SDS-PAGE system. With respect to the remarkable frequency of infection to IBR in Iran and the necessity of controlling it through vaccination with recombinant vaccines of thymidine kinase, manufacturing and applying the recombinant gD protein are vital goals in recognition and distinction between infection and responsescaused by vaccine

Histomorphometric study of the effect of methionine on small intestine parameters in rat: an applied histologic study

Background: Assessment of morphological changes has more often been used in the diagnosis and assessment of intestinal pathology and development. Since methionine is widely used in nutritional and sports supplements and also there is not enough information about the effect of this amino acid on the gastrointestinal histomorphometry, the aim of this study was to assess the effect of methionine on the small intestine histomorphometry. Materials and methods: Thirty male Wistar rats were randomly divided to three equal groups. Two treatment groups received 100 and 200 mg/kg L-methionine solution respectively via intraperitoneal injection while the control group received normal saline. On day 21, all rats were euthanised and segments from three parts of small intestine were taken to histomorphometrical study. Paraffin sections were stained with haematoxylin and eosin, alcian blue (AB) and periodic acid Schiff (PAS) methods separately. In order to analyse histomorphometric features of each segment, villus height, width, area, crypt depth, villus height to crypt depth ratio, goblet cell number, and muscle layer thickness were measured. Results and conclusions: Obtained results revealed that methionine may change the histomorphometric parameters of small intestine

Anatomical transverse magnetic resonance imaging study of ligaments in palmar surface of metacarpus in Miniature donkey: identification of a new ligament

Background: Palmar region of metacarpus in the horses and donkeys is an important region because of its tendons and ligaments which contribute to stay apparatus. This study was done on forelimbs of 6 healthy Miniature donkeys to detect the tendons, ligaments and their accessories on the palmar surface of metacarpus in this animal. Materials and methods: Based on that magnetic resonance imaging (MRI) is a good technique to evaluate the soft tissues such as tendons and ligaments, palmar aspects of metacarpus in 6 euthanatised Miniature donkeys were prepared for anatomical and trans-sectional MRI studies to determine the tendons and ligaments in this region. Results: Suspensory ligament, deep digital flexor tendon and its inferior check ligament were similar to them in the horse. Superficial digital flexor tendon (SDFT) in this animal had superior check ligament that was present before the carpal joint. On the other hand in the Miniature donkey there was a second accessory ligament for the SDFT that originated from the proximal of palmar surface of the large metacarpal bone which we named it second accessory ligament of SDFT. This ligament was determined in the MRI images too. Conclusions: It seems that this ligament helps the Miniature donkey to stay apparatus, supporting more weight and load for a longer period of time and distance which is a specific morphological feature in this animal.

H-adaptive finite element analysis of consolidation problems in geomechanics

In this paper, a computational framework based on the h-adaptive finite element strategy is presented for the solution of consolidation problems in geomechanics. The efficiency and performance of alternative error estimation techniques is demonstrated via the analysis of a slope stability problem

E-learning adoption during COVID-19 crisis and its effect on achieving students’ performance: Evidence from business collages – Jordanian universities

This study aimed to know the effect of E-Learning with all its components on students’ performance at Business Colleges-Jordanian Universities during at COVID-19 crisis. To achieve study goals, a questionnaire was distributed to the students at Jordanian Universities, (870) valid questionnaires were recovered. The study found that: The level of E-Learning and its components at Jordanian Universities were at middle rates, students’ performance also comes at a moderate level. E-Learning and its components (Technological facilities readiness, Electronic applications readiness, Databases & E-resources readiness, People readiness, E-Learning management system readiness, Evaluation system readiness, teaching methods diversity, Educational curricula and electronic content readiness) have a significant effect on students’ performance at Jordanian Universities at significance (α ≤ 0.05). Whereas sub components of (E-Learning organizational environment readiness and legislations and regulations readiness) have no effect separately on students’ performance. The study recommended Jordanian Universities to improve E-Learning in all its components in the Universities, reinforce using E-Learning in education, and exploit the benefits of E-Learning to improve students’ performance because it is the main alternative to face emerging crises like CoronavirusPeer Reviewe

Radiological anatomy of distal phalanx of front foot in the pure Iranian Arabian horse

Background: Among the different breeds of horses, Asil horses with a five thousand year history have played an important role in human life. This study was designed as a result of the lack of information about the normal radiographic anatomy of this breed. Materials and methods: Radiography of the distal phalanx and associated soft tissue structures of the front feet of 10 healthy pure Iranian Arabian horses was performed on lateromedial radiographic projection to determine normal radiographic morphometry of this part. There were no problems in their limbs and in their history. Results: Obtained radiograph of each front distal phalanx was used to measure important distances, angles and ratios of the hoof wall. There was no significant difference between left and right digits for any radiographic determination. Conclusions: Measurements of this study differed from those reported in other breeds, so it can be used in the future as reference values for diagnosis of laminitis in front feet of Iranian Arabian horses.

Histomorphometric evaluation of tibial subchondral bone after moderate running in male and female Wistar rats

Background: Exercise has been shown to be beneficial to the skeleton, in both humans and animals. This study was done to test the sex-related difference in the risk of osteoarthritis (OA) of the knee joint and also on the subchondral bone after moderate running exercise. Materials and methods: Forty male and female Wistar rats were randomly assigned to four equal groups (2 male and 2 female groups) in the same condition. Ten animals of each sex were selected as control groups, while running exercises were performed in remaining 20 male and female rats, using a motor treadmill to motivate rats to run daily distances of 1 km at 5 days/week within 6 weeks. On day 43, all animals were sacrificed and the knee articular cartilage and also histomorphometric parameters of subchondral bone and mid shaft of tibia were evaluated. Results: Results showed mild OA in both male and female runner groups. Results in male runner rats were significantly lesser than that in female runners. On the other hand, the difference in female runner group showed significant changes in comparison with other groups in histomorphometric parameters in tibia. Conclusions: Obtained results showed that the development of knee OA and subchondral bone changes may be related to the sex differences. Although there was no synovitis in male runners, female runner group showed mild hyperaemia dropsy with a moderate synovitis in this region

Rodent models of neuroinflammation for Alzheimer\u27s disease

Alzheimer\u27s disease remains incurable, and the failures of current disease-modifying strategies for Alzheimer\u27s disease could be attributed to a lack of in vivo models that recapitulate the underlying etiology of late-onset Alzheimer\u27s disease. The etiology of late-onset Alzheimer\u27s disease is not based on mutations related to amyloid-beta (A beta) or tau production which are currently the basis of in vivo models of Alzheimer\u27s disease. It has recently been suggested that mechanisms like chronic neuroinflammation may occur prior to amyloid-beta and tau pathologies in late-onset Alzheimer\u27s disease. The aim of this study is to analyze the characteristics of rodent models of neuroinflammation in late-onset Alzheimer\u27s disease. Our search criteria were based on characteristics of an idealistic disease model that should recapitulate causes, symptoms, and lesions in a chronological order similar to the actual disease. Therefore, a model based on the inflammation hypothesis of late-onset Alzheimer\u27s disease should include the following features: (i) primary chronic neuroinflammation, (ii) manifestations of memory and cognitive impairment, and (iii) late development of tau and A beta pathologies. The following models fit the pre-defined criteria: lipopolysaccharide-and PolyI:C-induced models of immune challenge; streptozotocin-, okadaic acid-, and colchicine neurotoxin-induced neuroinflammation models, as well as interleukin-1 beta, anti-nerve growth factor and p25 transgenic models. Among these models, streptozotocin, PolyI:C-induced, and p25 neuroinflammation models are compatible with the inflammation hypothesis of Alzheimer\u27s disease